An improved cerulean fluorescent protein with enhanced brightness and reduced reversible photoswitching

Cyan fluorescent proteins (CFPs), such as Cerulean, are widely used as donor fluorophores in Förster resonance energy transfer (FRET) experiments. Nonetheless, the most widely used variants suffer from drawbacks that include low quantum yields and unstable flurorescence. To improve the fluorescence...

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Veröffentlicht in:	PloS one 2011-03, Vol.6 (3), p.e17896
Hauptverfasser:	Markwardt, Michele L, Kremers, Gert-Jan, Kraft, Catherine A, Ray, Krishanu, Cranfill, Paula J C, Wilson, Korey A, Day, Richard N, Wachter, Rebekka M, Davidson, Michael W, Rizzo, Megan A
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino acids Animals Biochemistry Biology Biotechnology Cell Biology Cell Survival Cells (biology) Chemical compounds Chemistry Chlorocebus aethiops Chromophores COS Cells Dwarfism Energy transfer Engineering Experiments Financial planners Fluorescence Fluorescence microscopy Fluorescence Resonance Energy Transfer Fluorophores Fusion protein Genetic engineering Genetic Vectors Genetics and Genomics HeLa Cells Humans Kinases Laboratories Lifetime Luminescent Proteins - metabolism Magnetic fields Medicine Microscopy Mutagenesis Mutation Neuroscience Ophthalmology Optimization Physics Physiology Protein Engineering - methods Proteins Recombinant Proteins - metabolism Site-directed mutagenesis Time constant Transgenic animals Yield
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