Gene expression study of monocytes/macrophages during early foreign body reaction and identification of potential precursors of myofibroblasts
Foreign body reaction (FBR), initiated by adherence of macrophages to biomaterials, is associated with several complications. Searching for mechanisms potentially useful to overcome these complications, we have established the signaling role of monocytes/macrophages in the development of FBR and the...
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| Veröffentlicht in: | PloS one 2010-09, Vol.5 (9), p.e12949-e12949 |
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| creator | Mesure, Lindsay De Visscher, Geofrey Vranken, Ilse Lebacq, An Flameng, Willem |
| description | Foreign body reaction (FBR), initiated by adherence of macrophages to biomaterials, is associated with several complications. Searching for mechanisms potentially useful to overcome these complications, we have established the signaling role of monocytes/macrophages in the development of FBR and the presence of CD34(+) cells that potentially differentiate into myofibroblasts. Therefore, CD68(+) cells were in vitro activated with fibrinogen and also purified from the FBR after 3 days of implantation in rats. Gene expression profiles showed a switch from monocytes and macrophages attracted by fibrinogen to activated macrophages and eventually wound-healing macrophages. The immature FBR also contained a subpopulation of CD34(+) cells, which could be differentiated into myofibroblasts. This study showed that macrophages are the clear driving force of FBR, dependent on milieu, and myofibroblast deposition and differentiation. |
| doi_str_mv | 10.1371/journal.pone.0012949 |
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Searching for mechanisms potentially useful to overcome these complications, we have established the signaling role of monocytes/macrophages in the development of FBR and the presence of CD34(+) cells that potentially differentiate into myofibroblasts. Therefore, CD68(+) cells were in vitro activated with fibrinogen and also purified from the FBR after 3 days of implantation in rats. Gene expression profiles showed a switch from monocytes and macrophages attracted by fibrinogen to activated macrophages and eventually wound-healing macrophages. The immature FBR also contained a subpopulation of CD34(+) cells, which could be differentiated into myofibroblasts. This study showed that macrophages are the clear driving force of FBR, dependent on milieu, and myofibroblast deposition and differentiation.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0012949</identifier><identifier>PMID: 20886081</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Abdomen ; Animals ; Antibiotics ; Asthma ; Biological products ; Biomaterials ; Biomedical materials ; CD34 antigen ; Cell Biology/Cell Growth and Division ; Cell Biology/Leukocyte Signaling and Gene Expression ; Cell Differentiation ; Cells, Cultured ; Cloning ; Complications ; Disease Models, Animal ; Fibrin ; Fibrinogen ; Fibrinogen - immunology ; Fibroblasts ; Foreign-Body Reaction - genetics ; Foreign-Body Reaction - immunology ; Foreign-Body Reaction - physiopathology ; Gene Expression ; Gene Expression Profiling ; Genetic research ; Genetics and Genomics/Genetics of the Immune System ; Growth factors ; Heart surgery ; Humans ; Immunology/Genetics of the Immune System ; Implantation ; Ischemia ; Kinases ; Laboratory animals ; Macrophages ; Macrophages - cytology ; Macrophages - immunology ; Male ; Monocytes ; Monocytes - cytology ; Monocytes - immunology ; Myofibroblasts - cytology ; Myofibroblasts - immunology ; Precursors ; Rats ; Rats, Wistar ; Rodents ; Signaling ; Stem cells ; Stress response ; Surgical implants ; Transplants & implants ; Wound healing</subject><ispartof>PloS one, 2010-09, Vol.5 (9), p.e12949-e12949</ispartof><rights>COPYRIGHT 2010 Public Library of Science</rights><rights>2010 Mesure et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Mesure et al. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c757t-fdacd98d6e128ce12b202e7437fd230b7fb0d72ab37ffe31611b74800930f0173</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2944875/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2944875/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79472,79473</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20886081$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Gonçalves, Raquel</contributor><creatorcontrib>Mesure, Lindsay</creatorcontrib><creatorcontrib>De Visscher, Geofrey</creatorcontrib><creatorcontrib>Vranken, Ilse</creatorcontrib><creatorcontrib>Lebacq, An</creatorcontrib><creatorcontrib>Flameng, Willem</creatorcontrib><title>Gene expression study of monocytes/macrophages during early foreign body reaction and identification of potential precursors of myofibroblasts</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Foreign body reaction (FBR), initiated by adherence of macrophages to biomaterials, is associated with several complications. Searching for mechanisms potentially useful to overcome these complications, we have established the signaling role of monocytes/macrophages in the development of FBR and the presence of CD34(+) cells that potentially differentiate into myofibroblasts. Therefore, CD68(+) cells were in vitro activated with fibrinogen and also purified from the FBR after 3 days of implantation in rats. Gene expression profiles showed a switch from monocytes and macrophages attracted by fibrinogen to activated macrophages and eventually wound-healing macrophages. The immature FBR also contained a subpopulation of CD34(+) cells, which could be differentiated into myofibroblasts. 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cytology</subject><subject>Macrophages - immunology</subject><subject>Male</subject><subject>Monocytes</subject><subject>Monocytes - cytology</subject><subject>Monocytes - immunology</subject><subject>Myofibroblasts - cytology</subject><subject>Myofibroblasts - immunology</subject><subject>Precursors</subject><subject>Rats</subject><subject>Rats, Wistar</subject><subject>Rodents</subject><subject>Signaling</subject><subject>Stem cells</subject><subject>Stress response</subject><subject>Surgical implants</subject><subject>Transplants & implants</subject><subject>Wound healing</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9tq3DAQhk1padK0b1BaQ6GlF7vRwZbsm0IIbRoIBHq6FSNp5FXwWlvJLtmX6DNXTjYhW3pRDLYZffOP9GumKF5SsqRc0uOrMMUB-uUmDLgkhLK2ah8Vh7TlbCEY4Y8f_B8Uz1K6IqTmjRBPiwNGmkaQhh4Wv89wwBKvNxFT8mEo0zjZbRlcuQ5DMNsR0_EaTAybFXSYSjtFP3QlQuy3pQsRfTeUOuSUiGDGWQEGW3qLw-idN3ATynKbMM4h6MtcykwxhZhuymyD8zoG3UMa0_PiiYM-4Yvd96j4_unjt9PPi4vLs_PTk4uFkbUcF86CsW1jBVLWmPzSjDCUFZfOMk60dJpYyUDngENOBaVaVg0hLSeOUMmPite3ups-JLWzMqlsIuNV3YomE-e3hA1wpTbRryFuVQCvbgIhdgri6E2PStRCQwPooIKqBqMrClaLyiEwUWmetT7sqk16jdZkIyL0e6L7K4NfqS78UvlOq0bWWeDdTiCGnxOmUa19Mtj3MGCYkpK1EILRZt72m7_Ifx9uR3WQ9-8HF3JZM2uqk0ryppYtZZla_oPKj8W1N7ntnM_xvYT3ewmZGfF67GBKSZ1__fL_7OWPffbtA3aF0I-rFPpp7q20D1a3YG7YlCK6e48pUfPU3Lmh5qlRu6nJaa8e3s990t2Y8D_SVBbd</recordid><startdate>20100923</startdate><enddate>20100923</enddate><creator>Mesure, Lindsay</creator><creator>De Visscher, Geofrey</creator><creator>Vranken, Ilse</creator><creator>Lebacq, An</creator><creator>Flameng, Willem</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20100923</creationdate><title>Gene expression study of monocytes/macrophages during early foreign body reaction and identification of potential precursors of myofibroblasts</title><author>Mesure, Lindsay ; De Visscher, Geofrey ; Vranken, Ilse ; Lebacq, An ; Flameng, Willem</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c757t-fdacd98d6e128ce12b202e7437fd230b7fb0d72ab37ffe31611b74800930f0173</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Abdomen</topic><topic>Animals</topic><topic>Antibiotics</topic><topic>Asthma</topic><topic>Biological products</topic><topic>Biomaterials</topic><topic>Biomedical materials</topic><topic>CD34 antigen</topic><topic>Cell Biology/Cell Growth and Division</topic><topic>Cell Biology/Leukocyte Signaling and Gene Expression</topic><topic>Cell Differentiation</topic><topic>Cells, Cultured</topic><topic>Cloning</topic><topic>Complications</topic><topic>Disease Models, Animal</topic><topic>Fibrin</topic><topic>Fibrinogen</topic><topic>Fibrinogen - 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Searching for mechanisms potentially useful to overcome these complications, we have established the signaling role of monocytes/macrophages in the development of FBR and the presence of CD34(+) cells that potentially differentiate into myofibroblasts. Therefore, CD68(+) cells were in vitro activated with fibrinogen and also purified from the FBR after 3 days of implantation in rats. Gene expression profiles showed a switch from monocytes and macrophages attracted by fibrinogen to activated macrophages and eventually wound-healing macrophages. The immature FBR also contained a subpopulation of CD34(+) cells, which could be differentiated into myofibroblasts. This study showed that macrophages are the clear driving force of FBR, dependent on milieu, and myofibroblast deposition and differentiation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>20886081</pmid><doi>10.1371/journal.pone.0012949</doi><tpages>e12949</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Abdomen Animals Antibiotics Asthma Biological products Biomaterials Biomedical materials CD34 antigen Cell Biology/Cell Growth and Division Cell Biology/Leukocyte Signaling and Gene Expression Cell Differentiation Cells, Cultured Cloning Complications Disease Models, Animal Fibrin Fibrinogen Fibrinogen - immunology Fibroblasts Foreign-Body Reaction - genetics Foreign-Body Reaction - immunology Foreign-Body Reaction - physiopathology Gene Expression Gene Expression Profiling Genetic research Genetics and Genomics/Genetics of the Immune System Growth factors Heart surgery Humans Immunology/Genetics of the Immune System Implantation Ischemia Kinases Laboratory animals Macrophages Macrophages - cytology Macrophages - immunology Male Monocytes Monocytes - cytology Monocytes - immunology Myofibroblasts - cytology Myofibroblasts - immunology Precursors Rats Rats, Wistar Rodents Signaling Stem cells Stress response Surgical implants Transplants & implants Wound healing |
| title | Gene expression study of monocytes/macrophages during early foreign body reaction and identification of potential precursors of myofibroblasts |
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