Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences
CTX-M-producing Escherichia coli strains are regarded as major global pathogens. The nucleotide sequence of three plasmids (pEC_B24: 73801-bp; pEC_L8: 118525-bp and pEC_L46: 144871-bp) from Escherichia coli isolates obtained from patients with urinary tract infections and one plasmid (pEC_Bactec: 92...
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| Veröffentlicht in: | PloS one 2010-06, Vol.5 (6), p.e11202-e11202 |
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| creator | Smet, Annemieke Van Nieuwerburgh, Filip Vandekerckhove, Tom T M Martel, An Deforce, Dieter Butaye, Patrick Haesebrouck, Freddy |
| description | CTX-M-producing Escherichia coli strains are regarded as major global pathogens.
The nucleotide sequence of three plasmids (pEC_B24: 73801-bp; pEC_L8: 118525-bp and pEC_L46: 144871-bp) from Escherichia coli isolates obtained from patients with urinary tract infections and one plasmid (pEC_Bactec: 92970-bp) from an Escherichia coli strain isolated from the joint of a horse with arthritis were determined. Plasmid pEC_Bactec belongs to the IncI1 group and carries two resistance genes: bla(TEM-1) and bla(CTX-M-15). It shares more than 90% homology with a previously published bla(CTX-M)-plasmid from E. coli of human origin. Plasmid pEC_B24 belongs to the IncFII group whereas plasmids pEC_L8 and pEC_L46 represent a fusion of two replicons of type FII and FIA. On the pEC_B24 backbone, two resistance genes, bla(TEM-1) and bla(CTX-M-15), were found. Six resistance genes, bla(TEM-1), bla(CTX-M-15), bla(OXA-1), aac6'-lb-cr, tetA and catB4, were detected on the pEC_L8 backbone. The same antimicrobial drug resistance genes, with the exception of tetA, were also identified on the pEC_L46 backbone. Genome analysis of all 4 plasmids studied provides evidence of a seemingly frequent transposition event of the bla(CTX-M-15)-ISEcp1 element. This element seems to have a preferred insertion site at the tnpA gene of a bla(TEM)-carrying Tn3-like transposon, the latter itself being inserted by a transposition event. The IS26-composite transposon, which contains the bla(OXA-1), aac6'-lb-cr and catB4 genes, was inserted into plasmids pEC_L8 and pEC_L46 by homologous recombination rather than a transposition event. Results obtained for pEC_L46 indicated that IS26 also plays an important role in structural rearrangements of the plasmid backbone and seems to facilitate the mobilisation of fragments from other plasmids.
Collectively, these data suggests that IS26 together with ISEcp1 could play a critical role in the evolution of diverse multiresistant plasmids found in clinical Enterobacteriaceae. |
| doi_str_mv | 10.1371/journal.pone.0011202 |
| format | Article |
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The nucleotide sequence of three plasmids (pEC_B24: 73801-bp; pEC_L8: 118525-bp and pEC_L46: 144871-bp) from Escherichia coli isolates obtained from patients with urinary tract infections and one plasmid (pEC_Bactec: 92970-bp) from an Escherichia coli strain isolated from the joint of a horse with arthritis were determined. Plasmid pEC_Bactec belongs to the IncI1 group and carries two resistance genes: bla(TEM-1) and bla(CTX-M-15). It shares more than 90% homology with a previously published bla(CTX-M)-plasmid from E. coli of human origin. Plasmid pEC_B24 belongs to the IncFII group whereas plasmids pEC_L8 and pEC_L46 represent a fusion of two replicons of type FII and FIA. On the pEC_B24 backbone, two resistance genes, bla(TEM-1) and bla(CTX-M-15), were found. Six resistance genes, bla(TEM-1), bla(CTX-M-15), bla(OXA-1), aac6'-lb-cr, tetA and catB4, were detected on the pEC_L8 backbone. The same antimicrobial drug resistance genes, with the exception of tetA, were also identified on the pEC_L46 backbone. Genome analysis of all 4 plasmids studied provides evidence of a seemingly frequent transposition event of the bla(CTX-M-15)-ISEcp1 element. This element seems to have a preferred insertion site at the tnpA gene of a bla(TEM)-carrying Tn3-like transposon, the latter itself being inserted by a transposition event. The IS26-composite transposon, which contains the bla(OXA-1), aac6'-lb-cr and catB4 genes, was inserted into plasmids pEC_L8 and pEC_L46 by homologous recombination rather than a transposition event. Results obtained for pEC_L46 indicated that IS26 also plays an important role in structural rearrangements of the plasmid backbone and seems to facilitate the mobilisation of fragments from other plasmids.
Collectively, these data suggests that IS26 together with ISEcp1 could play a critical role in the evolution of diverse multiresistant plasmids found in clinical Enterobacteriaceae.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0011202</identifier><identifier>PMID: 20585456</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Antibiotics ; Antimicrobial agents ; Arthritis ; Backbone ; Bacteria ; Bacteriology ; Base sequence ; beta-Lactamases - genetics ; Binding sites ; Biotechnology ; Biotechnology/Applied Microbiology ; Coliforms ; DNA Transposable Elements ; Drug resistance ; E coli ; Enterobacteriaceae ; Enzymes ; Escherichia coli ; Escherichia coli - genetics ; Genes ; Genetics and Genomics/Bioinformatics ; Genetics and Genomics/Comparative Genomics ; Genetics and Genomics/Microbial Evolution and Genomics ; Genomes ; Genomics ; Health aspects ; Homologous recombination ; Homology ; Infectious Diseases/Antimicrobials and Drug Resistance ; Infectious Diseases/Bacterial Infections ; Insertion ; Insertion sequences ; Microbiology/Applied Microbiology ; Microbiology/Microbial Evolution and Genomics ; Nucleotide sequence ; Pathology ; Plasmids ; Proteins ; Public Health and Epidemiology/Infectious Diseases ; TnpA gene ; Transposition ; Transposons ; Urinary tract ; Urinary tract diseases ; Urinary tract infections ; Urogenital system ; Veterinary medicine</subject><ispartof>PloS one, 2010-06, Vol.5 (6), p.e11202-e11202</ispartof><rights>COPYRIGHT 2010 Public Library of Science</rights><rights>2010 Smet et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Smet et al. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c723t-c4c7a6a4baa091497c993329d72e5bf1f79d843c4ff1eb41d01195ed5a7b60783</citedby><cites>FETCH-LOGICAL-c723t-c4c7a6a4baa091497c993329d72e5bf1f79d843c4ff1eb41d01195ed5a7b60783</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2887853/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2887853/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2101,2927,23865,27923,27924,53790,53792,79471,79472</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20585456$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>DeLeo, Frank R.</contributor><creatorcontrib>Smet, Annemieke</creatorcontrib><creatorcontrib>Van Nieuwerburgh, Filip</creatorcontrib><creatorcontrib>Vandekerckhove, Tom T M</creatorcontrib><creatorcontrib>Martel, An</creatorcontrib><creatorcontrib>Deforce, Dieter</creatorcontrib><creatorcontrib>Butaye, Patrick</creatorcontrib><creatorcontrib>Haesebrouck, Freddy</creatorcontrib><title>Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>CTX-M-producing Escherichia coli strains are regarded as major global pathogens.
The nucleotide sequence of three plasmids (pEC_B24: 73801-bp; pEC_L8: 118525-bp and pEC_L46: 144871-bp) from Escherichia coli isolates obtained from patients with urinary tract infections and one plasmid (pEC_Bactec: 92970-bp) from an Escherichia coli strain isolated from the joint of a horse with arthritis were determined. Plasmid pEC_Bactec belongs to the IncI1 group and carries two resistance genes: bla(TEM-1) and bla(CTX-M-15). It shares more than 90% homology with a previously published bla(CTX-M)-plasmid from E. coli of human origin. Plasmid pEC_B24 belongs to the IncFII group whereas plasmids pEC_L8 and pEC_L46 represent a fusion of two replicons of type FII and FIA. On the pEC_B24 backbone, two resistance genes, bla(TEM-1) and bla(CTX-M-15), were found. Six resistance genes, bla(TEM-1), bla(CTX-M-15), bla(OXA-1), aac6'-lb-cr, tetA and catB4, were detected on the pEC_L8 backbone. The same antimicrobial drug resistance genes, with the exception of tetA, were also identified on the pEC_L46 backbone. Genome analysis of all 4 plasmids studied provides evidence of a seemingly frequent transposition event of the bla(CTX-M-15)-ISEcp1 element. This element seems to have a preferred insertion site at the tnpA gene of a bla(TEM)-carrying Tn3-like transposon, the latter itself being inserted by a transposition event. The IS26-composite transposon, which contains the bla(OXA-1), aac6'-lb-cr and catB4 genes, was inserted into plasmids pEC_L8 and pEC_L46 by homologous recombination rather than a transposition event. Results obtained for pEC_L46 indicated that IS26 also plays an important role in structural rearrangements of the plasmid backbone and seems to facilitate the mobilisation of fragments from other plasmids.
Collectively, these data suggests that IS26 together with ISEcp1 could play a critical role in the evolution of diverse multiresistant plasmids found in clinical Enterobacteriaceae.</description><subject>Antibiotics</subject><subject>Antimicrobial agents</subject><subject>Arthritis</subject><subject>Backbone</subject><subject>Bacteria</subject><subject>Bacteriology</subject><subject>Base sequence</subject><subject>beta-Lactamases - genetics</subject><subject>Binding sites</subject><subject>Biotechnology</subject><subject>Biotechnology/Applied Microbiology</subject><subject>Coliforms</subject><subject>DNA Transposable Elements</subject><subject>Drug resistance</subject><subject>E coli</subject><subject>Enterobacteriaceae</subject><subject>Enzymes</subject><subject>Escherichia coli</subject><subject>Escherichia coli - genetics</subject><subject>Genes</subject><subject>Genetics and Genomics/Bioinformatics</subject><subject>Genetics and Genomics/Comparative Genomics</subject><subject>Genetics and Genomics/Microbial Evolution and Genomics</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Health aspects</subject><subject>Homologous recombination</subject><subject>Homology</subject><subject>Infectious Diseases/Antimicrobials and Drug Resistance</subject><subject>Infectious Diseases/Bacterial Infections</subject><subject>Insertion</subject><subject>Insertion sequences</subject><subject>Microbiology/Applied Microbiology</subject><subject>Microbiology/Microbial Evolution and Genomics</subject><subject>Nucleotide sequence</subject><subject>Pathology</subject><subject>Plasmids</subject><subject>Proteins</subject><subject>Public Health and Epidemiology/Infectious Diseases</subject><subject>TnpA gene</subject><subject>Transposition</subject><subject>Transposons</subject><subject>Urinary tract</subject><subject>Urinary tract diseases</subject><subject>Urinary tract infections</subject><subject>Urogenital 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Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Smet, Annemieke</au><au>Van Nieuwerburgh, Filip</au><au>Vandekerckhove, Tom T M</au><au>Martel, An</au><au>Deforce, Dieter</au><au>Butaye, Patrick</au><au>Haesebrouck, Freddy</au><au>DeLeo, Frank R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2010-06-18</date><risdate>2010</risdate><volume>5</volume><issue>6</issue><spage>e11202</spage><epage>e11202</epage><pages>e11202-e11202</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>CTX-M-producing Escherichia coli strains are regarded as major global pathogens.
The nucleotide sequence of three plasmids (pEC_B24: 73801-bp; pEC_L8: 118525-bp and pEC_L46: 144871-bp) from Escherichia coli isolates obtained from patients with urinary tract infections and one plasmid (pEC_Bactec: 92970-bp) from an Escherichia coli strain isolated from the joint of a horse with arthritis were determined. Plasmid pEC_Bactec belongs to the IncI1 group and carries two resistance genes: bla(TEM-1) and bla(CTX-M-15). It shares more than 90% homology with a previously published bla(CTX-M)-plasmid from E. coli of human origin. Plasmid pEC_B24 belongs to the IncFII group whereas plasmids pEC_L8 and pEC_L46 represent a fusion of two replicons of type FII and FIA. On the pEC_B24 backbone, two resistance genes, bla(TEM-1) and bla(CTX-M-15), were found. Six resistance genes, bla(TEM-1), bla(CTX-M-15), bla(OXA-1), aac6'-lb-cr, tetA and catB4, were detected on the pEC_L8 backbone. The same antimicrobial drug resistance genes, with the exception of tetA, were also identified on the pEC_L46 backbone. Genome analysis of all 4 plasmids studied provides evidence of a seemingly frequent transposition event of the bla(CTX-M-15)-ISEcp1 element. This element seems to have a preferred insertion site at the tnpA gene of a bla(TEM)-carrying Tn3-like transposon, the latter itself being inserted by a transposition event. The IS26-composite transposon, which contains the bla(OXA-1), aac6'-lb-cr and catB4 genes, was inserted into plasmids pEC_L8 and pEC_L46 by homologous recombination rather than a transposition event. Results obtained for pEC_L46 indicated that IS26 also plays an important role in structural rearrangements of the plasmid backbone and seems to facilitate the mobilisation of fragments from other plasmids.
Collectively, these data suggests that IS26 together with ISEcp1 could play a critical role in the evolution of diverse multiresistant plasmids found in clinical Enterobacteriaceae.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>20585456</pmid><doi>10.1371/journal.pone.0011202</doi><tpages>e11202</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2010-06, Vol.5 (6), p.e11202-e11202 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1292303503 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS); EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Antibiotics Antimicrobial agents Arthritis Backbone Bacteria Bacteriology Base sequence beta-Lactamases - genetics Binding sites Biotechnology Biotechnology/Applied Microbiology Coliforms DNA Transposable Elements Drug resistance E coli Enterobacteriaceae Enzymes Escherichia coli Escherichia coli - genetics Genes Genetics and Genomics/Bioinformatics Genetics and Genomics/Comparative Genomics Genetics and Genomics/Microbial Evolution and Genomics Genomes Genomics Health aspects Homologous recombination Homology Infectious Diseases/Antimicrobials and Drug Resistance Infectious Diseases/Bacterial Infections Insertion Insertion sequences Microbiology/Applied Microbiology Microbiology/Microbial Evolution and Genomics Nucleotide sequence Pathology Plasmids Proteins Public Health and Epidemiology/Infectious Diseases TnpA gene Transposition Transposons Urinary tract Urinary tract diseases Urinary tract infections Urogenital system Veterinary medicine |
| title | Complete nucleotide sequence of CTX-M-15-plasmids from clinical Escherichia coli isolates: insertional events of transposons and insertion sequences |
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