Brain perihematoma genomic profile following spontaneous human intracerebral hemorrhage
Spontaneous intracerebral hemorrhage (ICH) represents about 15% of all strokes and is associated with high mortality rates. Our aim was to identify the gene expression changes and biological pathways altered in the brain following ICH. Twelve brain samples were obtained from four deceased patients w...
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| creator | Rosell, Anna Vilalta, Anna García-Berrocoso, Teresa Fernández-Cadenas, Israel Domingues-Montanari, Sophie Cuadrado, Eloy Delgado, Pilar Ribó, Marc Martínez-Sáez, Elena Ortega-Aznar, Arantxa Montaner, Joan |
| description | Spontaneous intracerebral hemorrhage (ICH) represents about 15% of all strokes and is associated with high mortality rates. Our aim was to identify the gene expression changes and biological pathways altered in the brain following ICH.
Twelve brain samples were obtained from four deceased patients who suffered an ICH including perihematomal tissue (PH) and the corresponding contralateral white (CW) and grey (CG) matter. Affymetrix GeneChip platform for analysis of over 47,000 transcripts was conducted. Microarray Analysis Suite 5.0 was used to process array images and the Ingenuity Pathway Analysis System was used to analyze biological mechanisms and functions of the genes. We identified 468 genes in the PH areas displaying a different expression pattern with a fold change between -3.74 and +5.16 when compared to the contralateral areas (291 overexpressed and 177 underexpressed). The top genes which appeared most significantly overexpressed in the PH areas codify for cytokines, chemokines, coagulation factors, cell growth and proliferation factors while the underexpressed codify for proteins involved in cell cycle or neurotrophins. Validation and replication studies at gene and protein level in brain samples confirmed microarray results.
The genomic responses identified in this study provide valuable information about potential biomarkers and target molecules altered in the perihematomal regions. |
| doi_str_mv | 10.1371/journal.pone.0016750 |
| format | Article |
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Twelve brain samples were obtained from four deceased patients who suffered an ICH including perihematomal tissue (PH) and the corresponding contralateral white (CW) and grey (CG) matter. Affymetrix GeneChip platform for analysis of over 47,000 transcripts was conducted. Microarray Analysis Suite 5.0 was used to process array images and the Ingenuity Pathway Analysis System was used to analyze biological mechanisms and functions of the genes. We identified 468 genes in the PH areas displaying a different expression pattern with a fold change between -3.74 and +5.16 when compared to the contralateral areas (291 overexpressed and 177 underexpressed). The top genes which appeared most significantly overexpressed in the PH areas codify for cytokines, chemokines, coagulation factors, cell growth and proliferation factors while the underexpressed codify for proteins involved in cell cycle or neurotrophins. Validation and replication studies at gene and protein level in brain samples confirmed microarray results.
The genomic responses identified in this study provide valuable information about potential biomarkers and target molecules altered in the perihematomal regions.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0016750</identifier><identifier>PMID: 21311749</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aged ; Aged, 80 and over ; Apoptosis ; Biology ; Biomarkers ; Brain ; Brain Diseases - etiology ; Brain Diseases - genetics ; Brain Diseases - metabolism ; Brain Diseases - pathology ; Brain research ; Cell cycle ; Cerebral Hemorrhage - complications ; Cerebral Hemorrhage - genetics ; Cerebral Hemorrhage - pathology ; Chemokines ; Coagulation ; Codification ; Cytokines ; DNA microarrays ; Edema ; Female ; Gene expression ; Gene Expression Profiling ; Genes ; Genome, Human ; Genomics ; Growth factors ; Health aspects ; Health care ; Hematoma - etiology ; Hematoma - genetics ; Hematoma - pathology ; Hemorrhage ; Humans ; Interleukin-8 - analysis ; Interleukin-8 - genetics ; Interleukin-8 - metabolism ; Intracerebral hemorrhage ; Laboratories ; Male ; Medicine ; Melatonin ; Mortality ; Multiple sclerosis ; Neurology ; Neuropathology ; Neurotrophic factors ; Neurotrophins ; Oligonucleotide Array Sequence Analysis ; Parkinson's disease ; Parkinsons disease ; Pathology ; Proteins ; Rodents ; Rupture, Spontaneous - complications ; Rupture, Spontaneous - genetics ; Rupture, Spontaneous - pathology ; Stroke ; Studies ; Traumatic brain injury ; Validation Studies as Topic</subject><ispartof>PloS one, 2011-02, Vol.6 (2), p.e16750-e16750</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Rosell et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>Rosell et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c723t-710871a4c781c071326bf4f29e9d9bf050edc56da1125c91236956577bc74f493</citedby><cites>FETCH-LOGICAL-c723t-710871a4c781c071326bf4f29e9d9bf050edc56da1125c91236956577bc74f493</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3032742/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3032742/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21311749$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Andreu, Antoni</contributor><creatorcontrib>Rosell, Anna</creatorcontrib><creatorcontrib>Vilalta, Anna</creatorcontrib><creatorcontrib>García-Berrocoso, Teresa</creatorcontrib><creatorcontrib>Fernández-Cadenas, Israel</creatorcontrib><creatorcontrib>Domingues-Montanari, Sophie</creatorcontrib><creatorcontrib>Cuadrado, Eloy</creatorcontrib><creatorcontrib>Delgado, Pilar</creatorcontrib><creatorcontrib>Ribó, Marc</creatorcontrib><creatorcontrib>Martínez-Sáez, Elena</creatorcontrib><creatorcontrib>Ortega-Aznar, Arantxa</creatorcontrib><creatorcontrib>Montaner, Joan</creatorcontrib><title>Brain perihematoma genomic profile following spontaneous human intracerebral hemorrhage</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Spontaneous intracerebral hemorrhage (ICH) represents about 15% of all strokes and is associated with high mortality rates. Our aim was to identify the gene expression changes and biological pathways altered in the brain following ICH.
Twelve brain samples were obtained from four deceased patients who suffered an ICH including perihematomal tissue (PH) and the corresponding contralateral white (CW) and grey (CG) matter. Affymetrix GeneChip platform for analysis of over 47,000 transcripts was conducted. Microarray Analysis Suite 5.0 was used to process array images and the Ingenuity Pathway Analysis System was used to analyze biological mechanisms and functions of the genes. We identified 468 genes in the PH areas displaying a different expression pattern with a fold change between -3.74 and +5.16 when compared to the contralateral areas (291 overexpressed and 177 underexpressed). The top genes which appeared most significantly overexpressed in the PH areas codify for cytokines, chemokines, coagulation factors, cell growth and proliferation factors while the underexpressed codify for proteins involved in cell cycle or neurotrophins. Validation and replication studies at gene and protein level in brain samples confirmed microarray results.
The genomic responses identified in this study provide valuable information about potential biomarkers and target molecules altered in the perihematomal regions.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Apoptosis</subject><subject>Biology</subject><subject>Biomarkers</subject><subject>Brain</subject><subject>Brain Diseases - etiology</subject><subject>Brain Diseases - genetics</subject><subject>Brain Diseases - metabolism</subject><subject>Brain Diseases - pathology</subject><subject>Brain research</subject><subject>Cell cycle</subject><subject>Cerebral Hemorrhage - complications</subject><subject>Cerebral Hemorrhage - genetics</subject><subject>Cerebral Hemorrhage - pathology</subject><subject>Chemokines</subject><subject>Coagulation</subject><subject>Codification</subject><subject>Cytokines</subject><subject>DNA microarrays</subject><subject>Edema</subject><subject>Female</subject><subject>Gene expression</subject><subject>Gene Expression 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one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rosell, Anna</au><au>Vilalta, Anna</au><au>García-Berrocoso, Teresa</au><au>Fernández-Cadenas, Israel</au><au>Domingues-Montanari, Sophie</au><au>Cuadrado, Eloy</au><au>Delgado, Pilar</au><au>Ribó, Marc</au><au>Martínez-Sáez, Elena</au><au>Ortega-Aznar, Arantxa</au><au>Montaner, Joan</au><au>Andreu, Antoni</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Brain perihematoma genomic profile following spontaneous human intracerebral hemorrhage</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2011-02-02</date><risdate>2011</risdate><volume>6</volume><issue>2</issue><spage>e16750</spage><epage>e16750</epage><pages>e16750-e16750</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Spontaneous intracerebral hemorrhage (ICH) represents about 15% of all strokes and is associated with high mortality rates. Our aim was to identify the gene expression changes and biological pathways altered in the brain following ICH.
Twelve brain samples were obtained from four deceased patients who suffered an ICH including perihematomal tissue (PH) and the corresponding contralateral white (CW) and grey (CG) matter. Affymetrix GeneChip platform for analysis of over 47,000 transcripts was conducted. Microarray Analysis Suite 5.0 was used to process array images and the Ingenuity Pathway Analysis System was used to analyze biological mechanisms and functions of the genes. We identified 468 genes in the PH areas displaying a different expression pattern with a fold change between -3.74 and +5.16 when compared to the contralateral areas (291 overexpressed and 177 underexpressed). The top genes which appeared most significantly overexpressed in the PH areas codify for cytokines, chemokines, coagulation factors, cell growth and proliferation factors while the underexpressed codify for proteins involved in cell cycle or neurotrophins. Validation and replication studies at gene and protein level in brain samples confirmed microarray results.
The genomic responses identified in this study provide valuable information about potential biomarkers and target molecules altered in the perihematomal regions.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21311749</pmid><doi>10.1371/journal.pone.0016750</doi><tpages>e16750</tpages><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1292255450 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aged Aged, 80 and over Apoptosis Biology Biomarkers Brain Brain Diseases - etiology Brain Diseases - genetics Brain Diseases - metabolism Brain Diseases - pathology Brain research Cell cycle Cerebral Hemorrhage - complications Cerebral Hemorrhage - genetics Cerebral Hemorrhage - pathology Chemokines Coagulation Codification Cytokines DNA microarrays Edema Female Gene expression Gene Expression Profiling Genes Genome, Human Genomics Growth factors Health aspects Health care Hematoma - etiology Hematoma - genetics Hematoma - pathology Hemorrhage Humans Interleukin-8 - analysis Interleukin-8 - genetics Interleukin-8 - metabolism Intracerebral hemorrhage Laboratories Male Medicine Melatonin Mortality Multiple sclerosis Neurology Neuropathology Neurotrophic factors Neurotrophins Oligonucleotide Array Sequence Analysis Parkinson's disease Parkinsons disease Pathology Proteins Rodents Rupture, Spontaneous - complications Rupture, Spontaneous - genetics Rupture, Spontaneous - pathology Stroke Studies Traumatic brain injury Validation Studies as Topic |
| title | Brain perihematoma genomic profile following spontaneous human intracerebral hemorrhage |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T14%3A00%3A11IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Brain%20perihematoma%20genomic%20profile%20following%20spontaneous%20human%20intracerebral%20hemorrhage&rft.jtitle=PloS%20one&rft.au=Rosell,%20Anna&rft.date=2011-02-02&rft.volume=6&rft.issue=2&rft.spage=e16750&rft.epage=e16750&rft.pages=e16750-e16750&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0016750&rft_dat=%3Cgale_plos_%3EA476906271%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1292255450&rft_id=info:pmid/21311749&rft_galeid=A476906271&rft_doaj_id=oai_doaj_org_article_051faeadb4ee4292912ae3ba1dc46c58&rfr_iscdi=true |