Ultrasound-mediated DNA transformation in thermophilic gram-positive anaerobes

Thermophilic, Gram-positive, anaerobic bacteria (TGPAs) are generally recalcitrant to chemical and electrotransformation due to their special cell-wall structure and the low intrinsic permeability of plasma membranes. Here we established for any Gram-positive or thermophiles an ultrasound-based sono...

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Veröffentlicht in:	PloS one 2010-09, Vol.5 (9), p.e12582-e12582
Hauptverfasser:	Lin, Lu, Song, Houhui, Ji, Yuetong, He, Zhili, Pu, Yunting, Zhou, Jizhong, Xu, Jian
Format:	Artikel
Sprache:	eng
Schlagworte:	Anaerobes Anaerobic bacteria Bacteria Bacteria, Anaerobic - genetics Bioprocessing Biotechnology/Applied Microbiology Biotechnology/Bioengineering Biotechnology/Environmental Microbiology Cell walls Cellulase Cellulose Clostridium Clostridium botulinum Clostridium thermocellum Consolidation Deoxyribonucleic acid Dextran Dextrans DNA DNA, Bacterial - genetics Efficiency Ethanol Fluorescence Gene therapy Gene Transfer Techniques - instrumentation Genes Genetic research Genetic transformation Genomics Green fluorescent protein Hexose Hot Temperature Kanamycin Laboratories Membrane permeability Membranes Metabolism Monosaccharides Mutagenesis Organisms Pentose Permeability Plasma membranes Plasmids Plasmids - genetics Proteins Shuttle vectors Thermoanaerobacter Thermoanaerobacter - genetics Thermophiles Thermophilic bacteria Transformation Transformation, Bacterial Ultrasonic imaging Ultrasonic methods Ultrasonic testing Ultrasonics Ultrasound
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