Protein C-terminal labeling and biotinylation using synthetic peptide and split-intein

Site-specific protein labeling or modification can facilitate the characterization of proteins with respect to their structure, folding, and interaction with other proteins. However, current methods of site-specific protein labeling are few and with limitations, therefore new methods are needed to s...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	PloS one 2009-12, Vol.4 (12), p.e8381-e8381
Hauptverfasser:	Volkmann, Gerrit, Liu, Xiang-Qin
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Amino acids Animals Biochemistry Biochemistry/Membrane Proteins and Energy Transduction Biochemistry/Protein Chemistry Biochemistry/Protein Folding Biotechnology/Protein Chemistry and Proteomics Biotin Biotinylation Cell Survival Cells (Biology) Chemical synthesis CHO Cells Cricetinae Cricetulus Enzymes Fluorescein Fluorescence Fluorescent Dyes - metabolism Folding Humans Immobilization Immobilized Proteins - metabolism Integrated circuits Inteins Labeling Labelling Labels Life sciences Ligands Maltose-Binding Proteins Mammalian cells Mass Spectrometry Methods Microchips Molecular biology Molecular Sequence Data Peptides Peptides - metabolism Periplasmic Binding Proteins - chemistry Polypeptides Protein C Protein engineering Protein folding Protein purification Protein transport Proteins Proteins - chemistry Proteins - isolation & purification Proteins - metabolism Receptors, Transferrin - metabolism Recombinant proteins Splicing Staining and Labeling - methods Streptavidin Synechocystis Trans-Splicing Transferrin Transferrins Vibrio harveyi
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Schreiben Sie den ersten Kommentar!