Resurrection of a Bull by Cloning from Organs Frozen without Cryoprotectant in a −80°C Freezer for a Decade

Frozen animal tissues without cryoprotectant have been thought to be inappropriate for use as a nuclear donor for somatic cell nuclear transfer (SCNT). We report the cloning of a bull using cells retrieved from testicles that had been taken from a dead animal and frozen without cryoprotectant in a −...

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Veröffentlicht in:	PloS one 2009-01, Vol.4 (1), p.e4142
Hauptverfasser:	Hoshino, Yoichiro, Hayashi, Noboru, Taniguchi, Shunji, Kobayashi, Naohiko, Sakai, Kenji, Otani, Tsuyoshi, Iritani, Akira, Saeki, Kazuhiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Animal tissues Animals Biotechnology Bos gaurus Bos javanicus bulls Calves Cattle Cell culture Cloning cloning (animals) Cords cryopreservation Cryoprotectants Cryoprotectors Developmental Biology/Cell Differentiation Developmental Biology/Embryology embryo transfer embryogenesis Embryos Endangered & extinct species Experiments Extinction Genetic engineering Genomes Genomics Livestock Nuclear transfer nuclear transplantation Organs Paternity pregnancy Rodents Somatic cell nuclear transfer spermatic cord testes
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