The impact of mouse passaging of Mycobacterium tuberculosis strains prior to virulence testing in the mouse and guinea pig aerosol models
It has been hypothesized that the virulence of lab-passaged Mycobacterium tuberculosis and recombinant M. tuberculosis mutants might be reduced due to multiple in vitro passages, and that virulence might be augmented by passage of these strains through mice before quantitative virulence testing in t...
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| Veröffentlicht in: | PloS one 2010-04, Vol.5 (4), p.e10289 |
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| creator | Converse, Paul J Eisenach, Kathleen D Theus, Sue A Nuermberger, Eric L Tyagi, Sandeep Ly, Lan H Geiman, Deborah E Guo, Haidan Nolan, Scott T Akar, Nicole C Klinkenberg, Lee G Gupta, Radhika Lun, Shichun Karakousis, Petros C Lamichhane, Gyanu McMurray, David N Grosset, Jacques H Bishai, William R |
| description | It has been hypothesized that the virulence of lab-passaged Mycobacterium tuberculosis and recombinant M. tuberculosis mutants might be reduced due to multiple in vitro passages, and that virulence might be augmented by passage of these strains through mice before quantitative virulence testing in the mouse or guinea pig aerosol models.
By testing three M. tuberculosis H37Rv samples, one deletion mutant, and one recent clinical isolate for survival by the quantitative organ CFU counting method in mouse or guinea pig aerosol or intravenous infection models, we could discern no increase in bacterial fitness as a result of passaging of M. tuberculosis strains in mice prior to quantitative virulence testing in two animal models. Surface lipid expression as assessed by neutral red staining and thin-layer chromatography for PDIM analysis also failed to identify virulence correlates.
These results indicate that animal passaging of M. tuberculosis strains prior to quantitative virulence testing in mouse or guinea pig models does not enhance or restore potency to strains that may have lost virulence due to in vitro passaging. It is critical to verify virulence of parental strains before genetic manipulations are undertaken and comparisons are made. |
| doi_str_mv | 10.1371/journal.pone.0010289 |
| format | Article |
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By testing three M. tuberculosis H37Rv samples, one deletion mutant, and one recent clinical isolate for survival by the quantitative organ CFU counting method in mouse or guinea pig aerosol or intravenous infection models, we could discern no increase in bacterial fitness as a result of passaging of M. tuberculosis strains in mice prior to quantitative virulence testing in two animal models. Surface lipid expression as assessed by neutral red staining and thin-layer chromatography for PDIM analysis also failed to identify virulence correlates.
These results indicate that animal passaging of M. tuberculosis strains prior to quantitative virulence testing in mouse or guinea pig models does not enhance or restore potency to strains that may have lost virulence due to in vitro passaging. It is critical to verify virulence of parental strains before genetic manipulations are undertaken and comparisons are made.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0010289</identifier><identifier>PMID: 20422019</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aerosol models ; Aerosols ; Analysis ; Animal models ; Animals ; Bacteria ; Bacteriological Techniques - standards ; Cattle ; Chromatography ; Deletion mutant ; Drug resistance ; Fitness ; Genetic engineering ; Genetic Fitness ; Guinea Pigs ; Infectious Diseases ; Infectious Diseases/Bacterial Infections ; Infectious Diseases/Respiratory Infections ; Intravenous administration ; Laboratories ; Lipids ; Lipids - analysis ; Medicine ; Mice ; Microbiology/Cellular Microbiology and Pathogenesis ; Microbiology/Microbial Growth and Development ; Models, Animal ; Mortality ; Mutagenesis ; Mutants ; Mycobacterium tuberculosis ; Mycobacterium tuberculosis - cytology ; Mycobacterium tuberculosis - genetics ; Mycobacterium tuberculosis - pathogenicity ; Nobel prizes ; Pathogenesis ; Permeability ; Reproductive fitness ; Research Design - standards ; Sequence Deletion ; Serial Passage ; Strains (organisms) ; Swine ; Thin layer chromatography ; Tuberculosis ; Tuberculosis - microbiology ; Virulence ; Virulence (Microbiology)</subject><ispartof>PloS one, 2010-04, Vol.5 (4), p.e10289</ispartof><rights>COPYRIGHT 2010 Public Library of Science</rights><rights>2010. This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c691t-78a187b2a8102187353015e9466bfd7843fc02d651ff093ed6327bf41095076f3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858211/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2858211/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79569,79570</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20422019$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Converse, Paul J</creatorcontrib><creatorcontrib>Eisenach, Kathleen D</creatorcontrib><creatorcontrib>Theus, Sue A</creatorcontrib><creatorcontrib>Nuermberger, Eric L</creatorcontrib><creatorcontrib>Tyagi, Sandeep</creatorcontrib><creatorcontrib>Ly, Lan H</creatorcontrib><creatorcontrib>Geiman, Deborah E</creatorcontrib><creatorcontrib>Guo, Haidan</creatorcontrib><creatorcontrib>Nolan, Scott T</creatorcontrib><creatorcontrib>Akar, Nicole C</creatorcontrib><creatorcontrib>Klinkenberg, Lee G</creatorcontrib><creatorcontrib>Gupta, Radhika</creatorcontrib><creatorcontrib>Lun, Shichun</creatorcontrib><creatorcontrib>Karakousis, Petros C</creatorcontrib><creatorcontrib>Lamichhane, Gyanu</creatorcontrib><creatorcontrib>McMurray, David N</creatorcontrib><creatorcontrib>Grosset, Jacques H</creatorcontrib><creatorcontrib>Bishai, William R</creatorcontrib><title>The impact of mouse passaging of Mycobacterium tuberculosis strains prior to virulence testing in the mouse and guinea pig aerosol models</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>It has been hypothesized that the virulence of lab-passaged Mycobacterium tuberculosis and recombinant M. tuberculosis mutants might be reduced due to multiple in vitro passages, and that virulence might be augmented by passage of these strains through mice before quantitative virulence testing in the mouse or guinea pig aerosol models.
By testing three M. tuberculosis H37Rv samples, one deletion mutant, and one recent clinical isolate for survival by the quantitative organ CFU counting method in mouse or guinea pig aerosol or intravenous infection models, we could discern no increase in bacterial fitness as a result of passaging of M. tuberculosis strains in mice prior to quantitative virulence testing in two animal models. Surface lipid expression as assessed by neutral red staining and thin-layer chromatography for PDIM analysis also failed to identify virulence correlates.
These results indicate that animal passaging of M. tuberculosis strains prior to quantitative virulence testing in mouse or guinea pig models does not enhance or restore potency to strains that may have lost virulence due to in vitro passaging. It is critical to verify virulence of parental strains before genetic manipulations are undertaken and comparisons are made.</description><subject>Aerosol models</subject><subject>Aerosols</subject><subject>Analysis</subject><subject>Animal models</subject><subject>Animals</subject><subject>Bacteria</subject><subject>Bacteriological Techniques - standards</subject><subject>Cattle</subject><subject>Chromatography</subject><subject>Deletion mutant</subject><subject>Drug resistance</subject><subject>Fitness</subject><subject>Genetic engineering</subject><subject>Genetic Fitness</subject><subject>Guinea Pigs</subject><subject>Infectious Diseases</subject><subject>Infectious Diseases/Bacterial Infections</subject><subject>Infectious Diseases/Respiratory Infections</subject><subject>Intravenous administration</subject><subject>Laboratories</subject><subject>Lipids</subject><subject>Lipids - analysis</subject><subject>Medicine</subject><subject>Mice</subject><subject>Microbiology/Cellular Microbiology and Pathogenesis</subject><subject>Microbiology/Microbial Growth and Development</subject><subject>Models, Animal</subject><subject>Mortality</subject><subject>Mutagenesis</subject><subject>Mutants</subject><subject>Mycobacterium tuberculosis</subject><subject>Mycobacterium tuberculosis - cytology</subject><subject>Mycobacterium tuberculosis - genetics</subject><subject>Mycobacterium tuberculosis - pathogenicity</subject><subject>Nobel prizes</subject><subject>Pathogenesis</subject><subject>Permeability</subject><subject>Reproductive fitness</subject><subject>Research Design - standards</subject><subject>Sequence Deletion</subject><subject>Serial Passage</subject><subject>Strains (organisms)</subject><subject>Swine</subject><subject>Thin layer chromatography</subject><subject>Tuberculosis</subject><subject>Tuberculosis - microbiology</subject><subject>Virulence</subject><subject>Virulence (Microbiology)</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqNk12L1DAUhoso7rr6D0QDguDFjPlo0_RGWBY_BlYWdPU2pPnoZGiTmqSL-xP812ac7jIFBelFwznPeZPzck5RPEdwjUiN3u78FJzo16N3eg0hgpg1D4pT1BC8ohiSh0fnk-JJjDsIK8IofVycYFhiDFFzWvy63mpgh1HIBLwBg5-iBqOIUXTWdfvQ51vp25zWwU4DSFOrg5x6H20EMQVhXQRjsD6A5MGNDVOvndQg6Zj2AtaBlG846AqnQDdZpwUYbQeEDj76PieV7uPT4pERfdTP5v9Z8e3D--uLT6vLq4-bi_PLlaQNSquaCcTqFguWO84nUhGIKt2UlLZG1awkRkKsaIWMgQ3RihJct6ZEsKlgTQ05K14edMfcBJ9djBxl-8qyYbTMxOZAKC92PDc3iHDLvbD8T8CHjouQrOx1roISQ8pqIXWpJGyVgtIo3JBaVIahrPVuvm1qB62kdtmzfiG6zDi75Z2_4ZhVDKO9wKtZIPgfU3b1H0-eqU7kV1lnfBaTg42Sn5c1YU1NCczU-i9U_pQerMxzZGyOLwreLAoyk_TP1IkpRr75-uX_2avvS_b1EbvVok_bPAlTst7FJVgeQJlHJQZt7p1DkO_X4M4Nvl8DPq9BLntx7Pp90d3ck9998ARH</recordid><startdate>20100421</startdate><enddate>20100421</enddate><creator>Converse, Paul J</creator><creator>Eisenach, Kathleen D</creator><creator>Theus, Sue A</creator><creator>Nuermberger, Eric L</creator><creator>Tyagi, Sandeep</creator><creator>Ly, Lan H</creator><creator>Geiman, Deborah E</creator><creator>Guo, Haidan</creator><creator>Nolan, Scott T</creator><creator>Akar, Nicole C</creator><creator>Klinkenberg, Lee G</creator><creator>Gupta, Radhika</creator><creator>Lun, Shichun</creator><creator>Karakousis, Petros C</creator><creator>Lamichhane, Gyanu</creator><creator>McMurray, David N</creator><creator>Grosset, Jacques H</creator><creator>Bishai, William R</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20100421</creationdate><title>The impact of mouse passaging of Mycobacterium tuberculosis strains prior to virulence testing in the mouse and guinea pig aerosol models</title><author>Converse, Paul J ; Eisenach, Kathleen D ; Theus, Sue A ; Nuermberger, Eric L ; Tyagi, Sandeep ; Ly, Lan H ; Geiman, Deborah E ; Guo, Haidan ; Nolan, Scott T ; Akar, Nicole C ; Klinkenberg, Lee G ; Gupta, Radhika ; Lun, Shichun ; Karakousis, Petros C ; Lamichhane, Gyanu ; McMurray, David N ; Grosset, Jacques H ; Bishai, William R</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c691t-78a187b2a8102187353015e9466bfd7843fc02d651ff093ed6327bf41095076f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aerosol models</topic><topic>Aerosols</topic><topic>Analysis</topic><topic>Animal models</topic><topic>Animals</topic><topic>Bacteria</topic><topic>Bacteriological Techniques - standards</topic><topic>Cattle</topic><topic>Chromatography</topic><topic>Deletion mutant</topic><topic>Drug resistance</topic><topic>Fitness</topic><topic>Genetic engineering</topic><topic>Genetic Fitness</topic><topic>Guinea Pigs</topic><topic>Infectious Diseases</topic><topic>Infectious Diseases/Bacterial Infections</topic><topic>Infectious Diseases/Respiratory Infections</topic><topic>Intravenous administration</topic><topic>Laboratories</topic><topic>Lipids</topic><topic>Lipids - analysis</topic><topic>Medicine</topic><topic>Mice</topic><topic>Microbiology/Cellular Microbiology and Pathogenesis</topic><topic>Microbiology/Microbial Growth and Development</topic><topic>Models, Animal</topic><topic>Mortality</topic><topic>Mutagenesis</topic><topic>Mutants</topic><topic>Mycobacterium tuberculosis</topic><topic>Mycobacterium tuberculosis - cytology</topic><topic>Mycobacterium tuberculosis - genetics</topic><topic>Mycobacterium tuberculosis - pathogenicity</topic><topic>Nobel prizes</topic><topic>Pathogenesis</topic><topic>Permeability</topic><topic>Reproductive fitness</topic><topic>Research Design - 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By testing three M. tuberculosis H37Rv samples, one deletion mutant, and one recent clinical isolate for survival by the quantitative organ CFU counting method in mouse or guinea pig aerosol or intravenous infection models, we could discern no increase in bacterial fitness as a result of passaging of M. tuberculosis strains in mice prior to quantitative virulence testing in two animal models. Surface lipid expression as assessed by neutral red staining and thin-layer chromatography for PDIM analysis also failed to identify virulence correlates.
These results indicate that animal passaging of M. tuberculosis strains prior to quantitative virulence testing in mouse or guinea pig models does not enhance or restore potency to strains that may have lost virulence due to in vitro passaging. It is critical to verify virulence of parental strains before genetic manipulations are undertaken and comparisons are made.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>20422019</pmid><doi>10.1371/journal.pone.0010289</doi><tpages>e10289</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aerosol models Aerosols Analysis Animal models Animals Bacteria Bacteriological Techniques - standards Cattle Chromatography Deletion mutant Drug resistance Fitness Genetic engineering Genetic Fitness Guinea Pigs Infectious Diseases Infectious Diseases/Bacterial Infections Infectious Diseases/Respiratory Infections Intravenous administration Laboratories Lipids Lipids - analysis Medicine Mice Microbiology/Cellular Microbiology and Pathogenesis Microbiology/Microbial Growth and Development Models, Animal Mortality Mutagenesis Mutants Mycobacterium tuberculosis Mycobacterium tuberculosis - cytology Mycobacterium tuberculosis - genetics Mycobacterium tuberculosis - pathogenicity Nobel prizes Pathogenesis Permeability Reproductive fitness Research Design - standards Sequence Deletion Serial Passage Strains (organisms) Swine Thin layer chromatography Tuberculosis Tuberculosis - microbiology Virulence Virulence (Microbiology) |
| title | The impact of mouse passaging of Mycobacterium tuberculosis strains prior to virulence testing in the mouse and guinea pig aerosol models |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-14T02%3A42%3A14IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20impact%20of%20mouse%20passaging%20of%20Mycobacterium%20tuberculosis%20strains%20prior%20to%20virulence%20testing%20in%20the%20mouse%20and%20guinea%20pig%20aerosol%20models&rft.jtitle=PloS%20one&rft.au=Converse,%20Paul%20J&rft.date=2010-04-21&rft.volume=5&rft.issue=4&rft.spage=e10289&rft.pages=e10289-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0010289&rft_dat=%3Cgale_plos_%3EA473897630%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1289449864&rft_id=info:pmid/20422019&rft_galeid=A473897630&rft_doaj_id=oai_doaj_org_article_120c20687ace4dc0bdd0cfd2937a5f81&rfr_iscdi=true |