Enhanced beetle luciferase for high-resolution bioluminescence imaging

We developed an enhanced green-emitting luciferase (ELuc) to be used as a bioluminescence imaging (BLI) probe. ELuc exhibits a light signal in mammalian cells that is over 10-fold stronger than that of the firefly luciferase (FLuc), which is the most widely used luciferase reporter gene. We showed t...

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Veröffentlicht in:	PloS one 2010-04, Vol.5 (4), p.e10011-e10011
Hauptverfasser:	Nakajima, Yoshihiro, Yamazaki, Tomomi, Nishii, Shigeaki, Noguchi, Takako, Hoshino, Hideto, Niwa, Kazuki, Viviani, Vadim R, Ohmiya, Yoshihiro
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Beetles Binding sites Bioluminescence Biotechnology Cell Biology/Gene Expression Cells (Biology) Circadian rhythm Cloning Coleoptera - enzymology Cytomegalovirus Diagnostic Imaging - methods Enzymes Gene expression Genes Humans Image resolution Karyopherins - analysis Karyopherins - metabolism Luciferase Luciferases Luminescent Measurements Luminescent Proteins Mammalian cells Microorganisms Molecular Biology Molecular Probe Techniques Molecular Probes Mutation Plasmids Protein Transport Proteins Proteins - analysis Reporter gene Science Temporal resolution Tissues Trends
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