Disordered microbial communities in asthmatic airways
A rich microbial environment in infancy protects against asthma [1], [2] and infections precipitate asthma exacerbations [3]. We compared the airway microbiota at three levels in adult patients with asthma, the related condition of COPD, and controls. We also studied bronchial lavage from asthmatic...
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| creator | Hilty, Markus Burke, Conor Pedro, Helder Cardenas, Paul Bush, Andy Bossley, Cara Davies, Jane Ervine, Aaron Poulter, Len Pachter, Lior Moffatt, Miriam F Cookson, William O C |
| description | A rich microbial environment in infancy protects against asthma [1], [2] and infections precipitate asthma exacerbations [3]. We compared the airway microbiota at three levels in adult patients with asthma, the related condition of COPD, and controls. We also studied bronchial lavage from asthmatic children and controls.
We identified 5,054 16S rRNA bacterial sequences from 43 subjects, detecting >70% of species present. The bronchial tree was not sterile, and contained a mean of 2,000 bacterial genomes per cm(2) surface sampled. Pathogenic Proteobacteria, particularly Haemophilus spp., were much more frequent in bronchi of adult asthmatics or patients with COPD than controls. We found similar highly significant increases in Proteobacteria in asthmatic children. Conversely, Bacteroidetes, particularly Prevotella spp., were more frequent in controls than adult or child asthmatics or COPD patients.
The results show the bronchial tree to contain a characteristic microbiota, and suggest that this microbiota is disturbed in asthmatic airways. |
| doi_str_mv | 10.1371/journal.pone.0008578 |
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We identified 5,054 16S rRNA bacterial sequences from 43 subjects, detecting >70% of species present. The bronchial tree was not sterile, and contained a mean of 2,000 bacterial genomes per cm(2) surface sampled. Pathogenic Proteobacteria, particularly Haemophilus spp., were much more frequent in bronchi of adult asthmatics or patients with COPD than controls. We found similar highly significant increases in Proteobacteria in asthmatic children. Conversely, Bacteroidetes, particularly Prevotella spp., were more frequent in controls than adult or child asthmatics or COPD patients.
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We identified 5,054 16S rRNA bacterial sequences from 43 subjects, detecting >70% of species present. The bronchial tree was not sterile, and contained a mean of 2,000 bacterial genomes per cm(2) surface sampled. Pathogenic Proteobacteria, particularly Haemophilus spp., were much more frequent in bronchi of adult asthmatics or patients with COPD than controls. We found similar highly significant increases in Proteobacteria in asthmatic children. Conversely, Bacteroidetes, particularly Prevotella spp., were more frequent in controls than adult or child asthmatics or COPD patients.
The results show the bronchial tree to contain a characteristic microbiota, and suggest that this microbiota is disturbed in asthmatic airways.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Alcohol</subject><subject>Asthma</subject><subject>Asthma - microbiology</subject><subject>Bacteria</subject><subject>Bacteria - classification</subject><subject>Bacteria - genetics</subject><subject>Bacteria - isolation & purification</subject><subject>Bacterial infections</subject><subject>Bioinformatics</subject><subject>Biopsy</subject><subject>Bronchi</subject><subject>Bronchoalveolar Lavage Fluid</subject><subject>Bronchus</subject><subject>Case-Control Studies</subject><subject>Child</subject><subject>Childhood asthma</subject><subject>Children</subject><subject>Chronic obstructive pulmonary disease</subject><subject>Comparative analysis</subject><subject>Disease</subject><subject>Female</subject><subject>Genes</subject><subject>Genomes</subject><subject>Genomics</subject><subject>Haplotypes</subject><subject>Health aspects</subject><subject>Heart</subject><subject>Hospitals</subject><subject>Humans</subject><subject>Male</subject><subject>Medical research</subject><subject>Microbial activity</subject><subject>Microbiology</subject><subject>Microbiota (Symbiotic organisms)</subject><subject>Microorganisms</subject><subject>Middle Aged</subject><subject>Neisseria</subject><subject>Pathogenesis</subject><subject>Patients</subject><subject>Personality traits</subject><subject>Phylogenetics</subject><subject>Respiratory Medicine/Asthma</subject><subject>Respiratory Medicine/COPD and Allied Disorders</subject><subject>Respiratory Medicine/Respiratory Pediatrics</subject><subject>Respiratory tract</subject><subject>RNA</subject><subject>RNA, Ribosomal, 16S - 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We compared the airway microbiota at three levels in adult patients with asthma, the related condition of COPD, and controls. We also studied bronchial lavage from asthmatic children and controls.
We identified 5,054 16S rRNA bacterial sequences from 43 subjects, detecting >70% of species present. The bronchial tree was not sterile, and contained a mean of 2,000 bacterial genomes per cm(2) surface sampled. Pathogenic Proteobacteria, particularly Haemophilus spp., were much more frequent in bronchi of adult asthmatics or patients with COPD than controls. We found similar highly significant increases in Proteobacteria in asthmatic children. Conversely, Bacteroidetes, particularly Prevotella spp., were more frequent in controls than adult or child asthmatics or COPD patients.
The results show the bronchial tree to contain a characteristic microbiota, and suggest that this microbiota is disturbed in asthmatic airways.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>20052417</pmid><doi>10.1371/journal.pone.0008578</doi><tpages>e8578</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Adolescent Adult Alcohol Asthma Asthma - microbiology Bacteria Bacteria - classification Bacteria - genetics Bacteria - isolation & purification Bacterial infections Bioinformatics Biopsy Bronchi Bronchoalveolar Lavage Fluid Bronchus Case-Control Studies Child Childhood asthma Children Chronic obstructive pulmonary disease Comparative analysis Disease Female Genes Genomes Genomics Haplotypes Health aspects Heart Hospitals Humans Male Medical research Microbial activity Microbiology Microbiota (Symbiotic organisms) Microorganisms Middle Aged Neisseria Pathogenesis Patients Personality traits Phylogenetics Respiratory Medicine/Asthma Respiratory Medicine/COPD and Allied Disorders Respiratory Medicine/Respiratory Pediatrics Respiratory tract RNA RNA, Ribosomal, 16S - genetics rRNA 16S Species Specificity Streptococcus infections Taxonomy Viral infections Young Adult |
| title | Disordered microbial communities in asthmatic airways |
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