The ELG1 clamp loader plays a role in sister chromatid cohesion
Mutations in the ELG1 gene of yeast lead to genomic instability, manifested in high levels of genetic recombination, chromosome loss, and gross chromosomal rearrangements. Elg1 shows similarity to the large subunit of the Replication Factor C clamp loader, and forms a RFC-like (RLC) complex in conju...
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| creator | Parnas, Oren Zipin-Roitman, Adi Mazor, Yuval Liefshitz, Batia Ben-Aroya, Shay Kupiec, Martin |
| description | Mutations in the ELG1 gene of yeast lead to genomic instability, manifested in high levels of genetic recombination, chromosome loss, and gross chromosomal rearrangements. Elg1 shows similarity to the large subunit of the Replication Factor C clamp loader, and forms a RFC-like (RLC) complex in conjunction with the 4 small RFC subunits. Two additional RLCs exist in yeast: in one of them the large subunit is Ctf18, and in the other, Rad24. Ctf18 has been characterized as the RLC that functions in sister chromatid cohesion. Here we present evidence that the Elg1 RLC (but not Rad24) also plays an important role in this process. A genetic screen identified the cohesin subunit Mcd1/Scc1 and its loader Scc2 as suppressors of the synthetic lethality between elg1 and ctf4. We describe genetic interactions between ELG1 and genes encoding cohesin subunits and their accessory proteins. We also show that defects in Elg1 lead to higher precocious sister chromatid separation, and that Ctf18 and Elg1 affect cohesion via a joint pathway. Finally, we localize both Ctf18 and Elg1 to chromatin and show that Elg1 plays a role in the recruitment of Ctf18. Our results suggest that Elg1, Ctf4, and Ctf18 may coordinate the relative movement of the replication fork with respect to the cohesin ring. |
| doi_str_mv | 10.1371/journal.pone.0005497 |
| format | Article |
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Elg1 shows similarity to the large subunit of the Replication Factor C clamp loader, and forms a RFC-like (RLC) complex in conjunction with the 4 small RFC subunits. Two additional RLCs exist in yeast: in one of them the large subunit is Ctf18, and in the other, Rad24. Ctf18 has been characterized as the RLC that functions in sister chromatid cohesion. Here we present evidence that the Elg1 RLC (but not Rad24) also plays an important role in this process. A genetic screen identified the cohesin subunit Mcd1/Scc1 and its loader Scc2 as suppressors of the synthetic lethality between elg1 and ctf4. We describe genetic interactions between ELG1 and genes encoding cohesin subunits and their accessory proteins. We also show that defects in Elg1 lead to higher precocious sister chromatid separation, and that Ctf18 and Elg1 affect cohesion via a joint pathway. Finally, we localize both Ctf18 and Elg1 to chromatin and show that Elg1 plays a role in the recruitment of Ctf18. 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This is an open-access article distributed under the terms of the Creative Commons Attribution License (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. 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| subjects | Baking yeast Blotting, Western Carrier Proteins - genetics Carrier Proteins - metabolism Carrier Proteins - physiology Cell Cycle Proteins - genetics Cell Cycle Proteins - metabolism Chromatids - physiology Chromatin Chromatin - metabolism Chromosomal Proteins, Non-Histone - genetics Chromosomal Proteins, Non-Histone - metabolism Chromosome rearrangements Chromosome Segregation Chromosomes Cohesin Cohesion DNA damage DNA-Binding Proteins - genetics DNA-Binding Proteins - metabolism Genetic aspects Genetic screening Genetics and Genomics/Chromosome Biology Genetics and Genomics/Disease Models Genetics and Genomics/Medical Genetics Genetics and Genomics/Nuclear Structure and Function Genomic instability Lethality Mutation Plasmids - genetics Protein Binding Proteins Recombination Recruitment Replication Replication factor C Saccharomyces cerevisiae Saccharomyces cerevisiae - growth & development Saccharomyces cerevisiae - metabolism Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism Saccharomyces cerevisiae Proteins - physiology Sister Chromatid Exchange Stability Suppressors Yeast |
| title | The ELG1 clamp loader plays a role in sister chromatid cohesion |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T05%3A40%3A25IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20ELG1%20clamp%20loader%20plays%20a%20role%20in%20sister%20chromatid%20cohesion&rft.jtitle=PloS%20one&rft.au=Parnas,%20Oren&rft.date=2009-05-11&rft.volume=4&rft.issue=5&rft.spage=e5497&rft.epage=e5497&rft.pages=e5497-e5497&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0005497&rft_dat=%3Cgale_plos_%3EA473168265%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1289181315&rft_id=info:pmid/19430531&rft_galeid=A473168265&rft_doaj_id=oai_doaj_org_article_50ec61e212b54c31927a891be1fbce54&rfr_iscdi=true |