Improved Models of Mini Anion Exchange Centrifugation Technique (mAECT) and Modified Single Centrifugation (MSC) for sleeping sickness diagnosis and staging
Human African trypanosomiasis (HAT), or sleeping sickness, is caused by two subspecies of the protozoan parasite Trypanosoma brucei (T.b.) and transmitted through tsetse flies (Glossina sp.). Capillary tube centrifugation (CTC, WOO) [5], quantitative buffy coat (QBC) [6], and mini anion exchange cen...
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| creator | Büscher, Philippe Mumba Ngoyi, Dieudonné Kaboré, Jacques Lejon, Veerle Robays, Jo Jamonneau, Vincent Bebronne, Nicolas Van der Veken, Wim Biéler, Sylvain |
| description | Human African trypanosomiasis (HAT), or sleeping sickness, is caused by two subspecies of the protozoan parasite Trypanosoma brucei (T.b.) and transmitted through tsetse flies (Glossina sp.). Capillary tube centrifugation (CTC, WOO) [5], quantitative buffy coat (QBC) [6], and mini anion exchange centrifugation technique (mAECT) [7] are applied on blood. mAECT is the most sensitive method for trypanosome detection in blood and is based on a purification technique first described by Lanham et al. and later adapted for diagnosis of sleeping sickness and of animal infections with T. brucei and T. evansi [7]-[10]. [...]quality control is performed internally at INRB and externally at ITM before a batch is released. |
| doi_str_mv | 10.1371/journal.pntd.0000471 |
| format | Article |
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Capillary tube centrifugation (CTC, WOO) [5], quantitative buffy coat (QBC) [6], and mini anion exchange centrifugation technique (mAECT) [7] are applied on blood. mAECT is the most sensitive method for trypanosome detection in blood and is based on a purification technique first described by Lanham et al. and later adapted for diagnosis of sleeping sickness and of animal infections with T. brucei and T. evansi [7]-[10]. 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This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Büscher P, Mumba Ngoyi D, Kaboré J, Lejon V, Robays J, et al. (2009) Improved Models of Mini Anion Exchange Centrifugation Technique (mAECT) and Modified Single Centrifugation (MSC) for Sleeping Sickness Diagnosis and Staging. 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Capillary tube centrifugation (CTC, WOO) [5], quantitative buffy coat (QBC) [6], and mini anion exchange centrifugation technique (mAECT) [7] are applied on blood. mAECT is the most sensitive method for trypanosome detection in blood and is based on a purification technique first described by Lanham et al. and later adapted for diagnosis of sleeping sickness and of animal infections with T. brucei and T. evansi [7]-[10]. [...]quality control is performed internally at INRB and externally at ITM before a batch is released.</description><subject>Anion exchange</subject><subject>Blood</subject><subject>Blood - parasitology</subject><subject>Centrifugation</subject><subject>Centrifugation - instrumentation</subject><subject>Centrifugation - methods</subject><subject>Cerebrospinal Fluid - parasitology</subject><subject>Chromatography, Ion Exchange - instrumentation</subject><subject>Chromatography, Ion Exchange - methods</subject><subject>From Innovation to Application</subject><subject>Health facilities</subject><subject>Humans</subject><subject>Infectious Diseases/Neglected Tropical Diseases</subject><subject>Infectious Diseases/Protozoal Infections</subject><subject>Infectious Diseases/Tropical and Travel-Associated Diseases</subject><subject>Insects</subject><subject>Parasites</subject><subject>Quality control</subject><subject>Tropical diseases</subject><subject>Trypanosoma brucei brucei - chemistry</subject><subject>Trypanosoma brucei brucei - 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Capillary tube centrifugation (CTC, WOO) [5], quantitative buffy coat (QBC) [6], and mini anion exchange centrifugation technique (mAECT) [7] are applied on blood. mAECT is the most sensitive method for trypanosome detection in blood and is based on a purification technique first described by Lanham et al. and later adapted for diagnosis of sleeping sickness and of animal infections with T. brucei and T. evansi [7]-[10]. [...]quality control is performed internally at INRB and externally at ITM before a batch is released.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>19936296</pmid><doi>10.1371/journal.pntd.0000471</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Anion exchange Blood Blood - parasitology Centrifugation Centrifugation - instrumentation Centrifugation - methods Cerebrospinal Fluid - parasitology Chromatography, Ion Exchange - instrumentation Chromatography, Ion Exchange - methods From Innovation to Application Health facilities Humans Infectious Diseases/Neglected Tropical Diseases Infectious Diseases/Protozoal Infections Infectious Diseases/Tropical and Travel-Associated Diseases Insects Parasites Quality control Tropical diseases Trypanosoma brucei brucei - chemistry Trypanosoma brucei brucei - isolation & purification Trypanosomiasis, African - diagnosis Trypanosomiasis, African - parasitology Vector-borne diseases |
| title | Improved Models of Mini Anion Exchange Centrifugation Technique (mAECT) and Modified Single Centrifugation (MSC) for sleeping sickness diagnosis and staging |
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