Transcriptional activation of the adenoviral genome is mediated by capsid protein VI
Gene expression of DNA viruses requires nuclear import of the viral genome. Human Adenoviruses (Ads), like most DNA viruses, encode factors within early transcription units promoting their own gene expression and counteracting cellular antiviral defense mechanisms. The cellular transcriptional repre...
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| Veröffentlicht in: | PLoS pathogens 2012-02, Vol.8 (2), p.e1002549-e1002549 |
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| creator | Schreiner, Sabrina Martinez, Ruben Groitl, Peter Rayne, Fabienne Vaillant, Remi Wimmer, Peter Bossis, Guillaume Sternsdorf, Thomas Marcinowski, Lisa Ruzsics, Zsolt Dobner, Thomas Wodrich, Harald |
| description | Gene expression of DNA viruses requires nuclear import of the viral genome. Human Adenoviruses (Ads), like most DNA viruses, encode factors within early transcription units promoting their own gene expression and counteracting cellular antiviral defense mechanisms. The cellular transcriptional repressor Daxx prevents viral gene expression through the assembly of repressive chromatin remodeling complexes targeting incoming viral genomes. However, it has remained unclear how initial transcriptional activation of the adenoviral genome is achieved. Here we show that Daxx mediated repression of the immediate early Ad E1A promoter is efficiently counteracted by the capsid protein VI. This requires a conserved PPxY motif in protein VI. Capsid proteins from other DNA viruses were also shown to activate the Ad E1A promoter independent of Ad gene expression and support virus replication. Our results show how Ad entry is connected to transcriptional activation of their genome in the nucleus. Our data further suggest a common principle for genome activation of DNA viruses by counteracting Daxx related repressive mechanisms through virion proteins. |
| doi_str_mv | 10.1371/journal.ppat.1002549 |
| format | Article |
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Human Adenoviruses (Ads), like most DNA viruses, encode factors within early transcription units promoting their own gene expression and counteracting cellular antiviral defense mechanisms. The cellular transcriptional repressor Daxx prevents viral gene expression through the assembly of repressive chromatin remodeling complexes targeting incoming viral genomes. However, it has remained unclear how initial transcriptional activation of the adenoviral genome is achieved. Here we show that Daxx mediated repression of the immediate early Ad E1A promoter is efficiently counteracted by the capsid protein VI. This requires a conserved PPxY motif in protein VI. Capsid proteins from other DNA viruses were also shown to activate the Ad E1A promoter independent of Ad gene expression and support virus replication. Our results show how Ad entry is connected to transcriptional activation of their genome in the nucleus. Our data further suggest a common principle for genome activation of DNA viruses by counteracting Daxx related repressive mechanisms through virion proteins.</description><identifier>ISSN: 1553-7374</identifier><identifier>ISSN: 1553-7366</identifier><identifier>EISSN: 1553-7374</identifier><identifier>DOI: 10.1371/journal.ppat.1002549</identifier><identifier>PMID: 22427750</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adaptor Proteins, Signal Transducing - genetics ; Adaptor Proteins, Signal Transducing - physiology ; Adenoviridae - genetics ; Adenoviruses ; Amino Acid Motifs - genetics ; Amino Acid Motifs - physiology ; Apoptosis ; Biochemistry, Molecular Biology ; Biology ; Capsid Proteins - chemistry ; Capsid Proteins - genetics ; Capsid Proteins - metabolism ; Capsid Proteins - physiology ; Cells, Cultured ; Cytoplasm ; Gene expression ; Gene Expression Regulation, Viral ; Genes, Viral - physiology ; Genetic aspects ; Genetic Fitness - physiology ; Genetic transcription ; Genome, Viral - genetics ; Genomes ; Genomics ; Health aspects ; Humans ; Life Sciences ; Microbiology and Parasitology ; Mutant Proteins - chemistry ; Mutant Proteins - genetics ; Mutant Proteins - metabolism ; Mutant Proteins - physiology ; Nuclear Proteins - genetics ; Nuclear Proteins - physiology ; Physiological aspects ; Proteins ; Transcription Factors - genetics ; Transcription Factors - metabolism ; Transcription Factors - physiology ; Transcriptional Activation - genetics ; Transfection ; Viral proteins ; Viral Proteins - chemistry ; Viral Proteins - metabolism ; Viral Proteins - physiology ; Virology ; Virus Replication - genetics ; Viruses</subject><ispartof>PLoS pathogens, 2012-02, Vol.8 (2), p.e1002549-e1002549</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>2012 Schreiner et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Schreiner S, Martinez R, Groitl P, Rayne F, Vaillant R, et al. (2012) Transcriptional Activation of the Adenoviral Genome Is Mediated by Capsid Protein VI. 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Human Adenoviruses (Ads), like most DNA viruses, encode factors within early transcription units promoting their own gene expression and counteracting cellular antiviral defense mechanisms. The cellular transcriptional repressor Daxx prevents viral gene expression through the assembly of repressive chromatin remodeling complexes targeting incoming viral genomes. However, it has remained unclear how initial transcriptional activation of the adenoviral genome is achieved. Here we show that Daxx mediated repression of the immediate early Ad E1A promoter is efficiently counteracted by the capsid protein VI. This requires a conserved PPxY motif in protein VI. Capsid proteins from other DNA viruses were also shown to activate the Ad E1A promoter independent of Ad gene expression and support virus replication. Our results show how Ad entry is connected to transcriptional activation of their genome in the nucleus. Our data further suggest a common principle for genome activation of DNA viruses by counteracting Daxx related repressive mechanisms through virion proteins.</description><subject>Adaptor Proteins, Signal Transducing - genetics</subject><subject>Adaptor Proteins, Signal Transducing - physiology</subject><subject>Adenoviridae - genetics</subject><subject>Adenoviruses</subject><subject>Amino Acid Motifs - genetics</subject><subject>Amino Acid Motifs - physiology</subject><subject>Apoptosis</subject><subject>Biochemistry, Molecular Biology</subject><subject>Biology</subject><subject>Capsid Proteins - chemistry</subject><subject>Capsid Proteins - genetics</subject><subject>Capsid Proteins - metabolism</subject><subject>Capsid Proteins - physiology</subject><subject>Cells, Cultured</subject><subject>Cytoplasm</subject><subject>Gene expression</subject><subject>Gene Expression Regulation, Viral</subject><subject>Genes, Viral - physiology</subject><subject>Genetic aspects</subject><subject>Genetic Fitness - 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Human Adenoviruses (Ads), like most DNA viruses, encode factors within early transcription units promoting their own gene expression and counteracting cellular antiviral defense mechanisms. The cellular transcriptional repressor Daxx prevents viral gene expression through the assembly of repressive chromatin remodeling complexes targeting incoming viral genomes. However, it has remained unclear how initial transcriptional activation of the adenoviral genome is achieved. Here we show that Daxx mediated repression of the immediate early Ad E1A promoter is efficiently counteracted by the capsid protein VI. This requires a conserved PPxY motif in protein VI. Capsid proteins from other DNA viruses were also shown to activate the Ad E1A promoter independent of Ad gene expression and support virus replication. Our results show how Ad entry is connected to transcriptional activation of their genome in the nucleus. Our data further suggest a common principle for genome activation of DNA viruses by counteracting Daxx related repressive mechanisms through virion proteins.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22427750</pmid><doi>10.1371/journal.ppat.1002549</doi><oa>free_for_read</oa></addata></record> |
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| subjects | Adaptor Proteins, Signal Transducing - genetics Adaptor Proteins, Signal Transducing - physiology Adenoviridae - genetics Adenoviruses Amino Acid Motifs - genetics Amino Acid Motifs - physiology Apoptosis Biochemistry, Molecular Biology Biology Capsid Proteins - chemistry Capsid Proteins - genetics Capsid Proteins - metabolism Capsid Proteins - physiology Cells, Cultured Cytoplasm Gene expression Gene Expression Regulation, Viral Genes, Viral - physiology Genetic aspects Genetic Fitness - physiology Genetic transcription Genome, Viral - genetics Genomes Genomics Health aspects Humans Life Sciences Microbiology and Parasitology Mutant Proteins - chemistry Mutant Proteins - genetics Mutant Proteins - metabolism Mutant Proteins - physiology Nuclear Proteins - genetics Nuclear Proteins - physiology Physiological aspects Proteins Transcription Factors - genetics Transcription Factors - metabolism Transcription Factors - physiology Transcriptional Activation - genetics Transfection Viral proteins Viral Proteins - chemistry Viral Proteins - metabolism Viral Proteins - physiology Virology Virus Replication - genetics Viruses |
| title | Transcriptional activation of the adenoviral genome is mediated by capsid protein VI |
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