Effect of labeling with iron oxide particles or nanodiamonds on the functionality of adipose-derived mesenchymal stem cells
Stem cells are increasingly the focus of translational research as well as having emerging roles in human cellular therapy. To support these uses there is a need for improved methods for in vivo cell localization and tracking. In this study, we examined the effects of cell labeling on the in vitro f...
Gespeichert in:
| Veröffentlicht in: | PloS one 2013-01, Vol.8 (1), p.e52997 |
|---|---|
| Hauptverfasser: | , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | |
|---|---|
| container_issue | 1 |
| container_start_page | e52997 |
| container_title | PloS one |
| container_volume | 8 |
| creator | Blaber, Sinead P Hill, Cameron J Webster, Rebecca A Say, Jana M Brown, Louise J Wang, Shih-Chang Vesey, Graham Herbert, Benjamin Ross |
| description | Stem cells are increasingly the focus of translational research as well as having emerging roles in human cellular therapy. To support these uses there is a need for improved methods for in vivo cell localization and tracking. In this study, we examined the effects of cell labeling on the in vitro functionality of human adipose-derived mesenchymal stem cells. Our results provide a basis for future in vivo studies investigating implanted cell fate and longevity. In particular, we investigated the effects of two different particles: micron-sized (~0.9 µm) fluorescently labeled (Dragon Green) superparamagnetic iron oxide particles (M-SPIO particles); and, carboxylated nanodiamonds of ~0.25 µm in size. The effects of labeling on the functionality of adipose-derived MSCs were assessed by in vitro morphology, osteogenic and adipogenic differentiation potential, CD marker expression, cytokine secretion profiling and quantitative proteomics of the intra-cellular proteome. The differentiation and CD marker assays for stem-like functionality were not altered upon label incorporation and no secreted or intra-cellular protein changes indicative of stress or toxicity were detected. These in vitro results indicate that the M-SPIO particles and nanodiamonds investigated in this study are biocompatible with MSCs and therefore would be suitable labels for cell localization and tracking in vivo. |
| doi_str_mv | 10.1371/journal.pone.0052997 |
| format | Article |
| fullrecord | <record><control><sourceid>gale_plos_</sourceid><recordid>TN_cdi_plos_journals_1289072316</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><galeid>A478439674</galeid><doaj_id>oai_doaj_org_article_007f4a430cb6446594926d88678bd338</doaj_id><sourcerecordid>A478439674</sourcerecordid><originalsourceid>FETCH-LOGICAL-c725t-3484ee61f8fbd32ac460389fb006d572a1bc31c34d9b56f991b2e820f8f864873</originalsourceid><addsrcrecordid>eNqNk1trFDEYhgdRbK3-A9GAIHqxa06bSW6EUqouFAqebkMmh92UmWQ7ydQu_nkz7rTsSi8kFzk935svb_JV1UsE54jU6MNVHPqg2vkmBjuHcIGFqB9Vx0gQPGMYksd746PqWUpXBSKcsafVESYEIojwcfX73DmrM4gOtKqxrQ8r8MvnNfB9DCDeemPBRvXZ69YmEHsQVIjGqy4GU-YB5LUFbgg6-1iy8Xk7SinjNzHZmbG9v7EGdDbZoNfbTrUgZdsBbds2Pa-eONUm-2LqT6ofn86_n32ZXVx-Xp6dXsx0jRd5Riin1jLkuGsMwUpTBgkXroGQmUWNFWo0QZpQI5oFc0KgBluOYeE5o7wmJ9Xrne6mjUlOviWJMBewxgSxQix3hInqSm5636l-K6Py8u9C7Fdy8kBCWDuqKIG6YZSyhaACM8M5q3nJjvCi9XE6bWg6a7QNuVftgejhTvBruYo3kiwI43AUeDcJ9PF6sCnLzqfRMBVsHEreRAgBKeekoG_-QR--3UStVLmADy6Wc_UoKk9pzSkRrKaFmj9AlWZs53X5ZM6X9YOA9wcBhcn2Nq_UkJJcfvv6_-zlz0P27R67tqrN6xTbYfxh6RCkO1D3MaXeunuTEZRjjdy5IccakVONlLBX-w90H3RXFOQPVyIMhg</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1289072316</pqid></control><display><type>article</type><title>Effect of labeling with iron oxide particles or nanodiamonds on the functionality of adipose-derived mesenchymal stem cells</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Public Library of Science (PLoS) Journals Open Access</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central</source><source>Free Full-Text Journals in Chemistry</source><creator>Blaber, Sinead P ; Hill, Cameron J ; Webster, Rebecca A ; Say, Jana M ; Brown, Louise J ; Wang, Shih-Chang ; Vesey, Graham ; Herbert, Benjamin Ross</creator><contributor>Rameshwar, Pranela</contributor><creatorcontrib>Blaber, Sinead P ; Hill, Cameron J ; Webster, Rebecca A ; Say, Jana M ; Brown, Louise J ; Wang, Shih-Chang ; Vesey, Graham ; Herbert, Benjamin Ross ; Rameshwar, Pranela</creatorcontrib><description>Stem cells are increasingly the focus of translational research as well as having emerging roles in human cellular therapy. To support these uses there is a need for improved methods for in vivo cell localization and tracking. In this study, we examined the effects of cell labeling on the in vitro functionality of human adipose-derived mesenchymal stem cells. Our results provide a basis for future in vivo studies investigating implanted cell fate and longevity. In particular, we investigated the effects of two different particles: micron-sized (~0.9 µm) fluorescently labeled (Dragon Green) superparamagnetic iron oxide particles (M-SPIO particles); and, carboxylated nanodiamonds of ~0.25 µm in size. The effects of labeling on the functionality of adipose-derived MSCs were assessed by in vitro morphology, osteogenic and adipogenic differentiation potential, CD marker expression, cytokine secretion profiling and quantitative proteomics of the intra-cellular proteome. The differentiation and CD marker assays for stem-like functionality were not altered upon label incorporation and no secreted or intra-cellular protein changes indicative of stress or toxicity were detected. These in vitro results indicate that the M-SPIO particles and nanodiamonds investigated in this study are biocompatible with MSCs and therefore would be suitable labels for cell localization and tracking in vivo.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0052997</identifier><identifier>PMID: 23301012</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adipocytes ; Adipocytes - cytology ; Animals ; Biocompatibility ; Biocompatible Materials - chemistry ; Biology ; Biomedical materials ; Carbon - chemistry ; Cell Adhesion ; Cell adhesion & migration ; Cell differentiation ; Cell fate ; Cell Lineage ; Cell Membrane - metabolism ; Cell Survival ; Cellular proteins ; Chemistry ; Coloring Agents - pharmacology ; Culture Media, Conditioned - pharmacology ; Dextrans - pharmacology ; Diamond - chemistry ; Diamonds ; Differentiation ; Ferric Compounds - pharmacology ; Ferric oxide ; Humans ; In vivo methods and tests ; Iron ; Iron oxides ; Labeling ; Labelling ; Labels ; Lipectomy ; Localization ; Magnetics ; Magnetite Nanoparticles ; Medicine ; Mesenchymal stem cells ; Mesenchymal Stem Cells - cytology ; Mesenchyme ; Nanoparticles - chemistry ; Nanostructure ; NMR ; Nuclear magnetic resonance ; Proteins ; Proteomics ; Proteomics - methods ; RNA polymerase ; Stem cell transplantation ; Stem cells ; Studies ; Surgical implants ; Toxicity ; Tracking</subject><ispartof>PloS one, 2013-01, Vol.8 (1), p.e52997</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Blaber et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Blaber et al 2013 Blaber et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c725t-3484ee61f8fbd32ac460389fb006d572a1bc31c34d9b56f991b2e820f8f864873</citedby><cites>FETCH-LOGICAL-c725t-3484ee61f8fbd32ac460389fb006d572a1bc31c34d9b56f991b2e820f8f864873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536808/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3536808/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23301012$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Rameshwar, Pranela</contributor><creatorcontrib>Blaber, Sinead P</creatorcontrib><creatorcontrib>Hill, Cameron J</creatorcontrib><creatorcontrib>Webster, Rebecca A</creatorcontrib><creatorcontrib>Say, Jana M</creatorcontrib><creatorcontrib>Brown, Louise J</creatorcontrib><creatorcontrib>Wang, Shih-Chang</creatorcontrib><creatorcontrib>Vesey, Graham</creatorcontrib><creatorcontrib>Herbert, Benjamin Ross</creatorcontrib><title>Effect of labeling with iron oxide particles or nanodiamonds on the functionality of adipose-derived mesenchymal stem cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Stem cells are increasingly the focus of translational research as well as having emerging roles in human cellular therapy. To support these uses there is a need for improved methods for in vivo cell localization and tracking. In this study, we examined the effects of cell labeling on the in vitro functionality of human adipose-derived mesenchymal stem cells. Our results provide a basis for future in vivo studies investigating implanted cell fate and longevity. In particular, we investigated the effects of two different particles: micron-sized (~0.9 µm) fluorescently labeled (Dragon Green) superparamagnetic iron oxide particles (M-SPIO particles); and, carboxylated nanodiamonds of ~0.25 µm in size. The effects of labeling on the functionality of adipose-derived MSCs were assessed by in vitro morphology, osteogenic and adipogenic differentiation potential, CD marker expression, cytokine secretion profiling and quantitative proteomics of the intra-cellular proteome. The differentiation and CD marker assays for stem-like functionality were not altered upon label incorporation and no secreted or intra-cellular protein changes indicative of stress or toxicity were detected. These in vitro results indicate that the M-SPIO particles and nanodiamonds investigated in this study are biocompatible with MSCs and therefore would be suitable labels for cell localization and tracking in vivo.</description><subject>Adipocytes</subject><subject>Adipocytes - cytology</subject><subject>Animals</subject><subject>Biocompatibility</subject><subject>Biocompatible Materials - chemistry</subject><subject>Biology</subject><subject>Biomedical materials</subject><subject>Carbon - chemistry</subject><subject>Cell Adhesion</subject><subject>Cell adhesion & migration</subject><subject>Cell differentiation</subject><subject>Cell fate</subject><subject>Cell Lineage</subject><subject>Cell Membrane - metabolism</subject><subject>Cell Survival</subject><subject>Cellular proteins</subject><subject>Chemistry</subject><subject>Coloring Agents - pharmacology</subject><subject>Culture Media, Conditioned - pharmacology</subject><subject>Dextrans - pharmacology</subject><subject>Diamond - chemistry</subject><subject>Diamonds</subject><subject>Differentiation</subject><subject>Ferric Compounds - pharmacology</subject><subject>Ferric oxide</subject><subject>Humans</subject><subject>In vivo methods and tests</subject><subject>Iron</subject><subject>Iron oxides</subject><subject>Labeling</subject><subject>Labelling</subject><subject>Labels</subject><subject>Lipectomy</subject><subject>Localization</subject><subject>Magnetics</subject><subject>Magnetite Nanoparticles</subject><subject>Medicine</subject><subject>Mesenchymal stem cells</subject><subject>Mesenchymal Stem Cells - cytology</subject><subject>Mesenchyme</subject><subject>Nanoparticles - chemistry</subject><subject>Nanostructure</subject><subject>NMR</subject><subject>Nuclear magnetic resonance</subject><subject>Proteins</subject><subject>Proteomics</subject><subject>Proteomics - methods</subject><subject>RNA polymerase</subject><subject>Stem cell transplantation</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Surgical implants</subject><subject>Toxicity</subject><subject>Tracking</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk1trFDEYhgdRbK3-A9GAIHqxa06bSW6EUqouFAqebkMmh92UmWQ7ydQu_nkz7rTsSi8kFzk935svb_JV1UsE54jU6MNVHPqg2vkmBjuHcIGFqB9Vx0gQPGMYksd746PqWUpXBSKcsafVESYEIojwcfX73DmrM4gOtKqxrQ8r8MvnNfB9DCDeemPBRvXZ69YmEHsQVIjGqy4GU-YB5LUFbgg6-1iy8Xk7SinjNzHZmbG9v7EGdDbZoNfbTrUgZdsBbds2Pa-eONUm-2LqT6ofn86_n32ZXVx-Xp6dXsx0jRd5Riin1jLkuGsMwUpTBgkXroGQmUWNFWo0QZpQI5oFc0KgBluOYeE5o7wmJ9Xrne6mjUlOviWJMBewxgSxQix3hInqSm5636l-K6Py8u9C7Fdy8kBCWDuqKIG6YZSyhaACM8M5q3nJjvCi9XE6bWg6a7QNuVftgejhTvBruYo3kiwI43AUeDcJ9PF6sCnLzqfRMBVsHEreRAgBKeekoG_-QR--3UStVLmADy6Wc_UoKk9pzSkRrKaFmj9AlWZs53X5ZM6X9YOA9wcBhcn2Nq_UkJJcfvv6_-zlz0P27R67tqrN6xTbYfxh6RCkO1D3MaXeunuTEZRjjdy5IccakVONlLBX-w90H3RXFOQPVyIMhg</recordid><startdate>20130103</startdate><enddate>20130103</enddate><creator>Blaber, Sinead P</creator><creator>Hill, Cameron J</creator><creator>Webster, Rebecca A</creator><creator>Say, Jana M</creator><creator>Brown, Louise J</creator><creator>Wang, Shih-Chang</creator><creator>Vesey, Graham</creator><creator>Herbert, Benjamin Ross</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130103</creationdate><title>Effect of labeling with iron oxide particles or nanodiamonds on the functionality of adipose-derived mesenchymal stem cells</title><author>Blaber, Sinead P ; Hill, Cameron J ; Webster, Rebecca A ; Say, Jana M ; Brown, Louise J ; Wang, Shih-Chang ; Vesey, Graham ; Herbert, Benjamin Ross</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c725t-3484ee61f8fbd32ac460389fb006d572a1bc31c34d9b56f991b2e820f8f864873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adipocytes</topic><topic>Adipocytes - cytology</topic><topic>Animals</topic><topic>Biocompatibility</topic><topic>Biocompatible Materials - chemistry</topic><topic>Biology</topic><topic>Biomedical materials</topic><topic>Carbon - chemistry</topic><topic>Cell Adhesion</topic><topic>Cell adhesion & migration</topic><topic>Cell differentiation</topic><topic>Cell fate</topic><topic>Cell Lineage</topic><topic>Cell Membrane - metabolism</topic><topic>Cell Survival</topic><topic>Cellular proteins</topic><topic>Chemistry</topic><topic>Coloring Agents - pharmacology</topic><topic>Culture Media, Conditioned - pharmacology</topic><topic>Dextrans - pharmacology</topic><topic>Diamond - chemistry</topic><topic>Diamonds</topic><topic>Differentiation</topic><topic>Ferric Compounds - pharmacology</topic><topic>Ferric oxide</topic><topic>Humans</topic><topic>In vivo methods and tests</topic><topic>Iron</topic><topic>Iron oxides</topic><topic>Labeling</topic><topic>Labelling</topic><topic>Labels</topic><topic>Lipectomy</topic><topic>Localization</topic><topic>Magnetics</topic><topic>Magnetite Nanoparticles</topic><topic>Medicine</topic><topic>Mesenchymal stem cells</topic><topic>Mesenchymal Stem Cells - cytology</topic><topic>Mesenchyme</topic><topic>Nanoparticles - chemistry</topic><topic>Nanostructure</topic><topic>NMR</topic><topic>Nuclear magnetic resonance</topic><topic>Proteins</topic><topic>Proteomics</topic><topic>Proteomics - methods</topic><topic>RNA polymerase</topic><topic>Stem cell transplantation</topic><topic>Stem cells</topic><topic>Studies</topic><topic>Surgical implants</topic><topic>Toxicity</topic><topic>Tracking</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blaber, Sinead P</creatorcontrib><creatorcontrib>Hill, Cameron J</creatorcontrib><creatorcontrib>Webster, Rebecca A</creatorcontrib><creatorcontrib>Say, Jana M</creatorcontrib><creatorcontrib>Brown, Louise J</creatorcontrib><creatorcontrib>Wang, Shih-Chang</creatorcontrib><creatorcontrib>Vesey, Graham</creatorcontrib><creatorcontrib>Herbert, Benjamin Ross</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - Academic</collection><collection>ProQuest Engineering Collection</collection><collection>ProQuest Biological Science Collection</collection><collection>Agricultural Science Database</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biological Science Database</collection><collection>Engineering Database</collection><collection>Nursing & Allied Health Premium</collection><collection>Advanced Technologies & Aerospace Database</collection><collection>ProQuest Advanced Technologies & Aerospace Collection</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Environmental Science Database</collection><collection>Materials Science Collection</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>ProQuest Central China</collection><collection>Engineering Collection</collection><collection>Environmental Science Collection</collection><collection>Genetics Abstracts</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blaber, Sinead P</au><au>Hill, Cameron J</au><au>Webster, Rebecca A</au><au>Say, Jana M</au><au>Brown, Louise J</au><au>Wang, Shih-Chang</au><au>Vesey, Graham</au><au>Herbert, Benjamin Ross</au><au>Rameshwar, Pranela</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Effect of labeling with iron oxide particles or nanodiamonds on the functionality of adipose-derived mesenchymal stem cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-01-03</date><risdate>2013</risdate><volume>8</volume><issue>1</issue><spage>e52997</spage><pages>e52997-</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Stem cells are increasingly the focus of translational research as well as having emerging roles in human cellular therapy. To support these uses there is a need for improved methods for in vivo cell localization and tracking. In this study, we examined the effects of cell labeling on the in vitro functionality of human adipose-derived mesenchymal stem cells. Our results provide a basis for future in vivo studies investigating implanted cell fate and longevity. In particular, we investigated the effects of two different particles: micron-sized (~0.9 µm) fluorescently labeled (Dragon Green) superparamagnetic iron oxide particles (M-SPIO particles); and, carboxylated nanodiamonds of ~0.25 µm in size. The effects of labeling on the functionality of adipose-derived MSCs were assessed by in vitro morphology, osteogenic and adipogenic differentiation potential, CD marker expression, cytokine secretion profiling and quantitative proteomics of the intra-cellular proteome. The differentiation and CD marker assays for stem-like functionality were not altered upon label incorporation and no secreted or intra-cellular protein changes indicative of stress or toxicity were detected. These in vitro results indicate that the M-SPIO particles and nanodiamonds investigated in this study are biocompatible with MSCs and therefore would be suitable labels for cell localization and tracking in vivo.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23301012</pmid><doi>10.1371/journal.pone.0052997</doi><tpages>e52997</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-01, Vol.8 (1), p.e52997 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1289072316 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adipocytes Adipocytes - cytology Animals Biocompatibility Biocompatible Materials - chemistry Biology Biomedical materials Carbon - chemistry Cell Adhesion Cell adhesion & migration Cell differentiation Cell fate Cell Lineage Cell Membrane - metabolism Cell Survival Cellular proteins Chemistry Coloring Agents - pharmacology Culture Media, Conditioned - pharmacology Dextrans - pharmacology Diamond - chemistry Diamonds Differentiation Ferric Compounds - pharmacology Ferric oxide Humans In vivo methods and tests Iron Iron oxides Labeling Labelling Labels Lipectomy Localization Magnetics Magnetite Nanoparticles Medicine Mesenchymal stem cells Mesenchymal Stem Cells - cytology Mesenchyme Nanoparticles - chemistry Nanostructure NMR Nuclear magnetic resonance Proteins Proteomics Proteomics - methods RNA polymerase Stem cell transplantation Stem cells Studies Surgical implants Toxicity Tracking |
| title | Effect of labeling with iron oxide particles or nanodiamonds on the functionality of adipose-derived mesenchymal stem cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-30T12%3A50%3A44IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Effect%20of%20labeling%20with%20iron%20oxide%20particles%20or%20nanodiamonds%20on%20the%20functionality%20of%20adipose-derived%20mesenchymal%20stem%20cells&rft.jtitle=PloS%20one&rft.au=Blaber,%20Sinead%20P&rft.date=2013-01-03&rft.volume=8&rft.issue=1&rft.spage=e52997&rft.pages=e52997-&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0052997&rft_dat=%3Cgale_plos_%3EA478439674%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1289072316&rft_id=info:pmid/23301012&rft_galeid=A478439674&rft_doaj_id=oai_doaj_org_article_007f4a430cb6446594926d88678bd338&rfr_iscdi=true |