The Wnt gatekeeper SFRP4 modulates EMT, cell migration and downstream Wnt signalling in serous ovarian cancer cells
Aberrant Wnt signalling is implicated in numerous human cancers, and understanding the effects of modulation of pathway members may lead to the development of novel therapeutics. Expression of secreted frizzled related protein 4 (SFRP4), an extracellular modulator of the Wnt signalling pathway, is p...
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| creator | Ford, Caroline E Jary, Eve Ma, Sean Si Qian Nixdorf, Sheri Heinzelmann-Schwarz, Viola A Ward, Robyn L |
| description | Aberrant Wnt signalling is implicated in numerous human cancers, and understanding the effects of modulation of pathway members may lead to the development of novel therapeutics. Expression of secreted frizzled related protein 4 (SFRP4), an extracellular modulator of the Wnt signalling pathway, is progressively lost in more aggressive ovarian cancer phenotypes. Here we show that recombinant SFRP4 (rSFRP4) treatment of a serous ovarian cancer cell line results in inhibition of β-catenin dependent Wnt signalling as measured by TOP/FOP Wnt reporter assay and decreased transcription of Wnt target genes, Axin2, CyclinD1 and Myc. In addition, rSFRP4 treatment significantly increased the ability of ovarian cancer cells to adhere to collagen and fibronectin, and decreased their ability to migrate across an inflicted wound. We conclude that these changes in cell behaviour may be mediated via mesenchymal to epithelial transition (MET), as rSFRP4 treatment also resulted in increased expression of the epithelial marker E-cadherin, and reduced expression of Vimentin and Twist. Combined, these results indicate that modulation of a single upstream gatekeeper of Wnt signalling can have effects on downstream Wnt signalling and ovarian cancer cell behaviour, as mediated through epithelial to mesenchymal plasticity (EMP). This raises the possibility that SFRP4 may be used both diagnostically and therapeutically in epithelial ovarian cancer. |
| doi_str_mv | 10.1371/journal.pone.0054362 |
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Expression of secreted frizzled related protein 4 (SFRP4), an extracellular modulator of the Wnt signalling pathway, is progressively lost in more aggressive ovarian cancer phenotypes. Here we show that recombinant SFRP4 (rSFRP4) treatment of a serous ovarian cancer cell line results in inhibition of β-catenin dependent Wnt signalling as measured by TOP/FOP Wnt reporter assay and decreased transcription of Wnt target genes, Axin2, CyclinD1 and Myc. In addition, rSFRP4 treatment significantly increased the ability of ovarian cancer cells to adhere to collagen and fibronectin, and decreased their ability to migrate across an inflicted wound. We conclude that these changes in cell behaviour may be mediated via mesenchymal to epithelial transition (MET), as rSFRP4 treatment also resulted in increased expression of the epithelial marker E-cadherin, and reduced expression of Vimentin and Twist. Combined, these results indicate that modulation of a single upstream gatekeeper of Wnt signalling can have effects on downstream Wnt signalling and ovarian cancer cell behaviour, as mediated through epithelial to mesenchymal plasticity (EMP). This raises the possibility that SFRP4 may be used both diagnostically and therapeutically in epithelial ovarian cancer.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0054362</identifier><identifier>PMID: 23326605</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Aberration ; beta Catenin - genetics ; beta Catenin - metabolism ; Biology ; Cancer ; Cancer cells ; Carcinoma, Ovarian Epithelial ; Care and treatment ; Cell cycle ; Cell Line, Tumor ; Cell migration ; Cell Movement - genetics ; Children & youth ; Childrens health ; Collagen ; Downstream effects ; Drug development ; E-cadherin ; Epithelial-Mesenchymal Transition ; Female ; Fibronectin ; Fibronectins ; Frizzled protein ; Genes ; Genetic Vectors ; Humans ; Intermediate filament proteins ; Low density lipoprotein receptors ; Medical prognosis ; Medical research ; Medicine ; Mesenchyme ; Metastasis ; Modulation ; Molecular Targeted Therapy ; Myc protein ; Neoplasms, Glandular and Epithelial - genetics ; Neoplasms, Glandular and Epithelial - pathology ; Ovarian cancer ; Ovarian carcinoma ; Ovarian Neoplasms - genetics ; Ovarian Neoplasms - pathology ; Penicillin ; Prostate cancer ; Proteins ; Proto-Oncogene Proteins - genetics ; Proto-Oncogene Proteins - metabolism ; Recombinant Proteins - genetics ; Recombinant Proteins - metabolism ; Signal transduction ; Signaling ; Stem cells ; Studies ; Transcription ; Transcription (Genetics) ; Transfection ; Tumors ; Vimentin ; Wnt protein ; Wnt Signaling Pathway - genetics ; Wounds ; β-Catenin</subject><ispartof>PloS one, 2013-01, Vol.8 (1), p.e54362-e54362</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Ford et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Ford et al 2013 Ford et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-70278c69642b196d7104787b8cec4699e949d258b02080d1a1ac4a18fb0b76423</citedby><cites>FETCH-LOGICAL-c692t-70278c69642b196d7104787b8cec4699e949d258b02080d1a1ac4a18fb0b76423</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543420/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3543420/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23326605$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Samant, Rajeev</contributor><creatorcontrib>Ford, Caroline E</creatorcontrib><creatorcontrib>Jary, Eve</creatorcontrib><creatorcontrib>Ma, Sean Si Qian</creatorcontrib><creatorcontrib>Nixdorf, Sheri</creatorcontrib><creatorcontrib>Heinzelmann-Schwarz, Viola A</creatorcontrib><creatorcontrib>Ward, Robyn L</creatorcontrib><title>The Wnt gatekeeper SFRP4 modulates EMT, cell migration and downstream Wnt signalling in serous ovarian cancer cells</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>Aberrant Wnt signalling is implicated in numerous human cancers, and understanding the effects of modulation of pathway members may lead to the development of novel therapeutics. Expression of secreted frizzled related protein 4 (SFRP4), an extracellular modulator of the Wnt signalling pathway, is progressively lost in more aggressive ovarian cancer phenotypes. Here we show that recombinant SFRP4 (rSFRP4) treatment of a serous ovarian cancer cell line results in inhibition of β-catenin dependent Wnt signalling as measured by TOP/FOP Wnt reporter assay and decreased transcription of Wnt target genes, Axin2, CyclinD1 and Myc. In addition, rSFRP4 treatment significantly increased the ability of ovarian cancer cells to adhere to collagen and fibronectin, and decreased their ability to migrate across an inflicted wound. We conclude that these changes in cell behaviour may be mediated via mesenchymal to epithelial transition (MET), as rSFRP4 treatment also resulted in increased expression of the epithelial marker E-cadherin, and reduced expression of Vimentin and Twist. Combined, these results indicate that modulation of a single upstream gatekeeper of Wnt signalling can have effects on downstream Wnt signalling and ovarian cancer cell behaviour, as mediated through epithelial to mesenchymal plasticity (EMP). This raises the possibility that SFRP4 may be used both diagnostically and therapeutically in epithelial ovarian cancer.</description><subject>Aberration</subject><subject>beta Catenin - genetics</subject><subject>beta Catenin - metabolism</subject><subject>Biology</subject><subject>Cancer</subject><subject>Cancer cells</subject><subject>Carcinoma, Ovarian Epithelial</subject><subject>Care and treatment</subject><subject>Cell cycle</subject><subject>Cell Line, Tumor</subject><subject>Cell migration</subject><subject>Cell Movement - genetics</subject><subject>Children & youth</subject><subject>Childrens health</subject><subject>Collagen</subject><subject>Downstream effects</subject><subject>Drug development</subject><subject>E-cadherin</subject><subject>Epithelial-Mesenchymal Transition</subject><subject>Female</subject><subject>Fibronectin</subject><subject>Fibronectins</subject><subject>Frizzled protein</subject><subject>Genes</subject><subject>Genetic Vectors</subject><subject>Humans</subject><subject>Intermediate filament proteins</subject><subject>Low density lipoprotein receptors</subject><subject>Medical prognosis</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Mesenchyme</subject><subject>Metastasis</subject><subject>Modulation</subject><subject>Molecular Targeted Therapy</subject><subject>Myc protein</subject><subject>Neoplasms, Glandular and Epithelial - genetics</subject><subject>Neoplasms, Glandular and Epithelial - pathology</subject><subject>Ovarian cancer</subject><subject>Ovarian carcinoma</subject><subject>Ovarian Neoplasms - genetics</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Penicillin</subject><subject>Prostate cancer</subject><subject>Proteins</subject><subject>Proto-Oncogene Proteins - genetics</subject><subject>Proto-Oncogene Proteins - metabolism</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - metabolism</subject><subject>Signal transduction</subject><subject>Signaling</subject><subject>Stem cells</subject><subject>Studies</subject><subject>Transcription</subject><subject>Transcription (Genetics)</subject><subject>Transfection</subject><subject>Tumors</subject><subject>Vimentin</subject><subject>Wnt protein</subject><subject>Wnt Signaling Pathway - genetics</subject><subject>Wounds</subject><subject>β-Catenin</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk9Fu0zAUhiMEYqPwBggsISGQaLFjx3FukKZpg0pDQ1uBS8txTlKPxC52MuDtcdZsatEukC9sHX_nt_0fnyR5TvCC0Jy8v3KDt6pdbJyFBcYZozx9kBySgqZznmL6cGd9kDwJ4SpCVHD-ODlIKU05x9lhElZrQN9tjxrVww-ADXh0eXrxhaHOVUMbgwGdfF69QxraFnWm8ao3ziJlK1S5Xzb0HlR3oxBME-_TGtsgY1EA74aA3LXyRlmkldVRelQJT5NHtWoDPJvmWfL19GR1_Gl-dv5xeXx0Nte8SPt5jtNcxCVnaUkKXuUEs1zkpdCgGS8KKFhRpZkocYoFrogiSjNFRF3iMo9JdJa83OpuWhfk5FeQJBUF5jmnIhLLLVE5dSU33nTK_5FOGXkTcL6RyvdGtyArnnJGmKBVlrMSmNJ1BgAcSEWyTOio9WE6bSg7qDTY3qt2T3R_x5q1bNy1pLF0LBZplryZBLz7OUDoZWfCaJiyEK2M985jiQVlRURf_YPe_7qJalR8gLG1i-fqUVQeRScpxZTmkVrcQ8VRQWd0_Fy1ifG9hLd7CZHp4XffqCEEuby8-H_2_Ns--3qHXYNq-3Vw7TD-t7APsi2ovQvBQ31nMsFy7I1bN-TYG3LqjZj2YrdAd0m3zUD_AlONCB8</recordid><startdate>20130111</startdate><enddate>20130111</enddate><creator>Ford, Caroline E</creator><creator>Jary, Eve</creator><creator>Ma, Sean Si Qian</creator><creator>Nixdorf, Sheri</creator><creator>Heinzelmann-Schwarz, Viola A</creator><creator>Ward, Robyn L</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130111</creationdate><title>The Wnt gatekeeper SFRP4 modulates EMT, cell migration and downstream Wnt signalling in serous ovarian cancer cells</title><author>Ford, Caroline E ; Jary, Eve ; Ma, Sean Si Qian ; Nixdorf, Sheri ; Heinzelmann-Schwarz, Viola A ; Ward, Robyn L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-70278c69642b196d7104787b8cec4699e949d258b02080d1a1ac4a18fb0b76423</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Aberration</topic><topic>beta Catenin - genetics</topic><topic>beta Catenin - metabolism</topic><topic>Biology</topic><topic>Cancer</topic><topic>Cancer cells</topic><topic>Carcinoma, Ovarian Epithelial</topic><topic>Care and treatment</topic><topic>Cell cycle</topic><topic>Cell Line, Tumor</topic><topic>Cell migration</topic><topic>Cell Movement - genetics</topic><topic>Children & youth</topic><topic>Childrens health</topic><topic>Collagen</topic><topic>Downstream effects</topic><topic>Drug development</topic><topic>E-cadherin</topic><topic>Epithelial-Mesenchymal Transition</topic><topic>Female</topic><topic>Fibronectin</topic><topic>Fibronectins</topic><topic>Frizzled protein</topic><topic>Genes</topic><topic>Genetic Vectors</topic><topic>Humans</topic><topic>Intermediate filament proteins</topic><topic>Low density lipoprotein receptors</topic><topic>Medical prognosis</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Mesenchyme</topic><topic>Metastasis</topic><topic>Modulation</topic><topic>Molecular Targeted Therapy</topic><topic>Myc protein</topic><topic>Neoplasms, Glandular and Epithelial - genetics</topic><topic>Neoplasms, Glandular and Epithelial - pathology</topic><topic>Ovarian cancer</topic><topic>Ovarian carcinoma</topic><topic>Ovarian Neoplasms - genetics</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Penicillin</topic><topic>Prostate cancer</topic><topic>Proteins</topic><topic>Proto-Oncogene Proteins - genetics</topic><topic>Proto-Oncogene Proteins - metabolism</topic><topic>Recombinant Proteins - genetics</topic><topic>Recombinant Proteins - metabolism</topic><topic>Signal transduction</topic><topic>Signaling</topic><topic>Stem cells</topic><topic>Studies</topic><topic>Transcription</topic><topic>Transcription (Genetics)</topic><topic>Transfection</topic><topic>Tumors</topic><topic>Vimentin</topic><topic>Wnt protein</topic><topic>Wnt Signaling Pathway - genetics</topic><topic>Wounds</topic><topic>β-Catenin</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ford, Caroline E</creatorcontrib><creatorcontrib>Jary, Eve</creatorcontrib><creatorcontrib>Ma, Sean Si Qian</creatorcontrib><creatorcontrib>Nixdorf, Sheri</creatorcontrib><creatorcontrib>Heinzelmann-Schwarz, Viola A</creatorcontrib><creatorcontrib>Ward, Robyn L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Gale In Context: Opposing Viewpoints</collection><collection>Gale In Context: Science</collection><collection>ProQuest Central (Corporate)</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Biotechnology Research Abstracts</collection><collection>Nursing & Allied Health Database</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Meteorological & Geoastrophysical Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Agricultural Science Collection</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>ProQuest Pharma Collection</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>ProQuest SciTech Collection</collection><collection>ProQuest Technology Collection</collection><collection>ProQuest Natural Science Collection</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>Materials Science & Engineering Collection</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>Advanced Technologies & Aerospace Collection</collection><collection>Agricultural & Environmental Science Collection</collection><collection>ProQuest Central Essentials</collection><collection>Biological Science Collection</collection><collection>ProQuest Central</collection><collection>Technology Collection</collection><collection>Natural Science Collection</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Materials Science Collection</collection><collection>ProQuest Central Korea</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>ProQuest Central Student</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>SciTech Premium Collection</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Materials Science Database</collection><collection>Nursing & Allied Health Database (Alumni Edition)</collection><collection>Meteorological & Geoastrophysical Abstracts - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ford, Caroline E</au><au>Jary, Eve</au><au>Ma, Sean Si Qian</au><au>Nixdorf, Sheri</au><au>Heinzelmann-Schwarz, Viola A</au><au>Ward, Robyn L</au><au>Samant, Rajeev</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Wnt gatekeeper SFRP4 modulates EMT, cell migration and downstream Wnt signalling in serous ovarian cancer cells</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-01-11</date><risdate>2013</risdate><volume>8</volume><issue>1</issue><spage>e54362</spage><epage>e54362</epage><pages>e54362-e54362</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>Aberrant Wnt signalling is implicated in numerous human cancers, and understanding the effects of modulation of pathway members may lead to the development of novel therapeutics. Expression of secreted frizzled related protein 4 (SFRP4), an extracellular modulator of the Wnt signalling pathway, is progressively lost in more aggressive ovarian cancer phenotypes. Here we show that recombinant SFRP4 (rSFRP4) treatment of a serous ovarian cancer cell line results in inhibition of β-catenin dependent Wnt signalling as measured by TOP/FOP Wnt reporter assay and decreased transcription of Wnt target genes, Axin2, CyclinD1 and Myc. In addition, rSFRP4 treatment significantly increased the ability of ovarian cancer cells to adhere to collagen and fibronectin, and decreased their ability to migrate across an inflicted wound. We conclude that these changes in cell behaviour may be mediated via mesenchymal to epithelial transition (MET), as rSFRP4 treatment also resulted in increased expression of the epithelial marker E-cadherin, and reduced expression of Vimentin and Twist. Combined, these results indicate that modulation of a single upstream gatekeeper of Wnt signalling can have effects on downstream Wnt signalling and ovarian cancer cell behaviour, as mediated through epithelial to mesenchymal plasticity (EMP). This raises the possibility that SFRP4 may be used both diagnostically and therapeutically in epithelial ovarian cancer.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23326605</pmid><doi>10.1371/journal.pone.0054362</doi><tpages>e54362</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-01, Vol.8 (1), p.e54362-e54362 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1289067638 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS) Journals Open Access; PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Aberration beta Catenin - genetics beta Catenin - metabolism Biology Cancer Cancer cells Carcinoma, Ovarian Epithelial Care and treatment Cell cycle Cell Line, Tumor Cell migration Cell Movement - genetics Children & youth Childrens health Collagen Downstream effects Drug development E-cadherin Epithelial-Mesenchymal Transition Female Fibronectin Fibronectins Frizzled protein Genes Genetic Vectors Humans Intermediate filament proteins Low density lipoprotein receptors Medical prognosis Medical research Medicine Mesenchyme Metastasis Modulation Molecular Targeted Therapy Myc protein Neoplasms, Glandular and Epithelial - genetics Neoplasms, Glandular and Epithelial - pathology Ovarian cancer Ovarian carcinoma Ovarian Neoplasms - genetics Ovarian Neoplasms - pathology Penicillin Prostate cancer Proteins Proto-Oncogene Proteins - genetics Proto-Oncogene Proteins - metabolism Recombinant Proteins - genetics Recombinant Proteins - metabolism Signal transduction Signaling Stem cells Studies Transcription Transcription (Genetics) Transfection Tumors Vimentin Wnt protein Wnt Signaling Pathway - genetics Wounds β-Catenin |
| title | The Wnt gatekeeper SFRP4 modulates EMT, cell migration and downstream Wnt signalling in serous ovarian cancer cells |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T10%3A07%3A04IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=The%20Wnt%20gatekeeper%20SFRP4%20modulates%20EMT,%20cell%20migration%20and%20downstream%20Wnt%20signalling%20in%20serous%20ovarian%20cancer%20cells&rft.jtitle=PloS%20one&rft.au=Ford,%20Caroline%20E&rft.date=2013-01-11&rft.volume=8&rft.issue=1&rft.spage=e54362&rft.epage=e54362&rft.pages=e54362-e54362&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0054362&rft_dat=%3Cgale_plos_%3EA478330337%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1289067638&rft_id=info:pmid/23326605&rft_galeid=A478330337&rft_doaj_id=oai_doaj_org_article_d62641483d574be4acf5eee6e1d1558c&rfr_iscdi=true |