QTL analysis using SNP markers developed by next-generation sequencing for identification of candidate genes controlling 4-methylthio-3-butenyl glucosinolate contents in roots of radish, Raphanus sativus L

SNP markers for QTL analysis of 4-MTB-GSL contents in radish roots were developed by determining nucleotide sequences of bulked PCR products using a next-generation sequencer. DNA fragments were amplified from two radish lines by multiplex PCR with six primer pairs, and those amplified by 2,880 prim...

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Veröffentlicht in:	PloS one 2013-01, Vol.8 (1), p.e53541
Hauptverfasser:	Zou, Zhongwei, Ishida, Masahiko, Li, Feng, Kakizaki, Tomohiro, Suzuki, Sho, Kitashiba, Hiroyasu, Nishio, Takeshi
Format:	Artikel
Sprache:	eng
Schlagworte:	2-Isopropylmalate Synthase - genetics 2-Isopropylmalate Synthase - metabolism Agriculture Aliphatic compounds Amino acids Arabidopsis Arabidopsis - genetics Arabidopsis - metabolism Arabidopsis thaliana Base Sequence Biology Biosynthesis Branched chain amino acids Brassica Brassica rapa Brassica rapa - genetics Brassica rapa - metabolism Brassicaceae Chain branching Chromosome Mapping Chromosomes, Plant Cultivars Cytochrome Deoxyribonucleic acid DNA DNA, Plant - genetics Expressed Sequence Tags Fragments Gene expression Gene sequencing Genes Genomes Genomics Genotype & phenotype Glucosinolates Glucosinolates - biosynthesis High-Throughput Nucleotide Sequencing Leucine Malate Dehydrogenase - genetics Malate Dehydrogenase - metabolism Markers Molecular Sequence Data Multiplex Polymerase Chain Reaction Multiplexing Nucleotide sequence Plant Proteins - genetics Plant Proteins - metabolism Plant Roots - genetics Plant Roots - metabolism Polymerase chain reaction Polymorphism, Single Nucleotide Quantitative genetics Quantitative Trait Loci Raphanus Raphanus - genetics Raphanus - metabolism Raphanus sativus Roots Science Seeds Single nucleotide polymorphisms Single-nucleotide polymorphism Synteny Transaminases - genetics Transaminases - metabolism
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