ADAM17 mediates MMP9 expression in lung epithelial cells

The purposes were to study the role of lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-α/nuclear factor-κB (NF-κB) signaling in matrix metalloproteinase 9 (MMP9) expression in A549 cells and to investigate the effects of lentivirus-mediated RNAi targeting of the disintegrin and metallop...

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Veröffentlicht in:	PloS one 2013-01, Vol.8 (1), p.e51701
Hauptverfasser:	Li, Ya-qing, Yan, Jian-ping, Xu, Wu-lin, Wang, Hong, Xia, Ying-jie, Wang, Hui-jun, Zhu, Yue-yan, Huang, Xiao-jun
Format:	Artikel
Sprache:	eng
Schlagworte:	ADAM Proteins - metabolism ADAM17 Protein Airway management Biology Cell Line Chronic obstructive pulmonary disease Cytokines Cytoplasm Deoxyribonucleic acid DNA Enzymes Epithelial cells Epithelial Cells - drug effects Epithelial Cells - enzymology Gastroenterology Gelatinase B Gene expression Gene Expression Regulation, Enzymologic - drug effects Genes Hospitals Humans I-kappa B Proteins - metabolism Kinases Laboratories Lentivirus - metabolism Lipopolysaccharides Lipopolysaccharides - pharmacology Lung - cytology Lungs Matrix metalloproteinase Matrix Metalloproteinase 9 - genetics Matrix Metalloproteinase 9 - metabolism Medicine Mitogens NF-kappa B - metabolism NF-KappaB Inhibitor alpha NF-κB protein Pathogenesis Phosphorylation Phosphorylation - drug effects Pyrrolidine Pyrrolidine dithiocarbamate Ribonucleic acid RNA RNA Interference - drug effects RNA, Messenger - genetics RNA, Messenger - metabolism RNA-mediated interference Rodents Signal Transduction - drug effects Signaling Synaptotagmin Time Factors Transcription Factor RelA - metabolism Transfection Tumor necrosis factor Tumor necrosis factor receptors Tumor Necrosis Factor-alpha - pharmacology Tumor necrosis factor-TNF Tumor necrosis factor-α
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Zusammenfassung:	The purposes were to study the role of lipopolysaccharide (LPS)-induced tumor necrosis factor (TNF)-α/nuclear factor-κB (NF-κB) signaling in matrix metalloproteinase 9 (MMP9) expression in A549 cells and to investigate the effects of lentivirus-mediated RNAi targeting of the disintegrin and metalloproteinase 17 (ADAM17) gene on LPS-induced MMP9 expression. MMP9 expression induced by LPS in A549 cells was significantly increased in a dose- and time-dependent manner (p
ISSN:	1932-6203 1932-6203
DOI:	10.1371/journal.pone.0051701