Simultaneous immunoassay analysis of plasma IL-6 and TNF-α on a microchip

Sandwich enzyme-linked immunosorbant assay (ELISA) using a 96-well plate is frequently employed for clinical diagnosis, but is time-and sample-consuming. To overcome these drawbacks, we performed a sandwich ELISA on a microchip. The microchip was made of cyclic olefin copolymer with 4 straight micro...

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Veröffentlicht in:	PloS one 2013, Vol.8 (1), p.e53620-e53620
Hauptverfasser:	Abe, Kaori, Hashimoto, Yoshiko, Yatsushiro, Shouki, Yamamura, Shohei, Bando, Mika, Hiroshima, Yuka, Kido, Jun-ichi, Tanaka, Masato, Shinohara, Yasuo, Ooie, Toshihiko, Baba, Yoshinobu, Kataoka, Masatoshi
Format:	Artikel
Sprache:	eng
Schlagworte:	Antigens Avidin Biology Blood Blood levels Cardiovascular disease Cytokines Dentistry Deposition Diagnosis Engineering Enzyme-Linked Immunosorbent Assay Enzymes Fixation Horseradish peroxidase Humans Immunoassay Immunoassay - methods Immunoglobulins Inkjet printing Interleukin 6 Interleukin-6 - blood Lab-On-A-Chip Devices Luminescence Luminescent Measurements Medical research Medicine Microchannels Peroxidase Piezoelectricity Plasma Polyolefins Printing Quantitative analysis Reagents Regression analysis Reproducibility Reproducibility of Results Sandwich structures Science Semiconductors Substrates Tumor Necrosis Factor-alpha - blood Tumor necrosis factor-TNF Tumor necrosis factor-α
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