Long-term imaging of Caenorhabditis elegans using nanoparticle-mediated immobilization

One advantage of the nematode Caenorhabditis elegans as a model organism is its suitability for in vivo optical microscopy. Imaging C. elegans often requires animals to be immobilized to avoid movement-related artifacts. Immobilization has been performed by application of anesthetics or by introduci...

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Veröffentlicht in:	PloS one 2013-01, Vol.8 (1), p.e53419
Hauptverfasser:	Kim, Eric, Sun, Lin, Gabel, Christopher V, Fang-Yen, Christopher
Format:	Artikel
Sprache:	eng
Schlagworte:	Anesthetics Animals Automation Axotomy Axotomy - instrumentation Axotomy - methods Beads Biocompatibility Bioengineering Biology Biophysics Caenorhabditis elegans Caenorhabditis elegans - metabolism Calcium Calcium Signaling Femtosecond Femtosecond lasers Immobilization Immobilization - instrumentation Immobilization - methods Light microscopy Materials Science Medical research Microfluidic Analytical Techniques Microfluidics Microscopy Microscopy - methods Movement Nanoparticles Nanoparticles - chemistry Nanotechnology - methods Nematodes Neurons Neurons - metabolism Optical microscopy Optics Physiological aspects Physiology Polystyrene Polystyrene resins Polystyrenes - chemistry Regrowth Roundworms Sepharose - chemistry Studies Toxic substances Worms
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