Effective preparation of Plasmodium vivax field isolates for high-throughput whole genome sequencing

Whole genome sequencing (WGS) of Plasmodium vivax is problematic due to the reliance on clinical isolates which are generally low in parasitaemia and sample volume. Furthermore, clinical isolates contain a significant contaminating background of host DNA which confounds efforts to map short read seq...

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Veröffentlicht in:	PloS one 2013-01, Vol.8 (1), p.e53160-e53160
Hauptverfasser:	Auburn, Sarah, Marfurt, Jutta, Maslen, Gareth, Campino, Susana, Ruano Rubio, Valentin, Manske, Magnus, Machunter, Barbara, Kenangalem, Enny, Noviyanti, Rintis, Trianty, Leily, Sebayang, Boni, Wirjanata, Grennady, Sriprawat, Kanlaya, Alcock, Daniel, Macinnis, Bronwyn, Miotto, Olivo, Clark, Taane G, Russell, Bruce, Anstey, Nicholas M, Nosten, François, Kwiatkowski, Dominic P, Price, Ric N
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis Bioinformatics Biology Blood Blood cells Cell culture Clinical isolates Clinical medicine Community development Contamination Copy number Councils Deoxyribonucleic acid DNA DNA sequencing DNA, Protozoan - genetics DNA, Protozoan - isolation & purification Erythrocytes Filtration Gene sequencing Genetic aspects Genetic testing Genome, Protozoan Genomes Genomics High-Throughput Nucleotide Sequencing - methods Humans Leukocytes Malaria Malaria, Vivax - diagnosis Malaria, Vivax - parasitology Medical research Medicine Molecular biology Nucleotide sequence Parasites Plasmodium Plasmodium falciparum Plasmodium vivax Plasmodium vivax - genetics Plasmodium vivax - isolation & purification Real-Time Polymerase Chain Reaction Red blood cells Single-nucleotide polymorphism Travellers Tropical diseases White blood cells
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