Expression of chicken DEC205 reflects the unique structure and function of the avian immune system
The generation of appropriate adaptive immune responses relies critically on dendritic cells, about which relatively little is known in chickens, a vital livestock species, in comparison with man and mouse. We cloned and sequenced chicken DEC205 cDNA and used this knowledge to produce quantitative P...
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| creator | Staines, Karen Young, John R Butter, Colin |
| description | The generation of appropriate adaptive immune responses relies critically on dendritic cells, about which relatively little is known in chickens, a vital livestock species, in comparison with man and mouse. We cloned and sequenced chicken DEC205 cDNA and used this knowledge to produce quantitative PCR assays and monoclonal antibodies to study expression of DEC205 as well as CD83. The gene structure of DEC205 was identical to those of other species. Transcripts of both genes were found at higher levels in lymphoid tissues and the expression of DEC205 in normal birds had a characteristic distribution in the primary lymphoid organs. In spleen, DEC205 was seen on cells ideally located to trap antigen. In thymus it was found on cells thought to participate in the education of T cells, and in the bursa on cells that may be involved in presentation of antigen to B cells and regulation of B cell migration. The expression of DEC205 on cells other than antigen presenting cells (APC) is also described. Isolated splenocytes strongly expressing DEC205 but not the KUL01 antigen have morphology similar to mammalian dendritic cells and the distinct expression of DEC205 within the avian-specific Bursa of Fabricius alludes to a unique function in this organ of B cell diversification. |
| doi_str_mv | 10.1371/journal.pone.0051799 |
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We cloned and sequenced chicken DEC205 cDNA and used this knowledge to produce quantitative PCR assays and monoclonal antibodies to study expression of DEC205 as well as CD83. The gene structure of DEC205 was identical to those of other species. Transcripts of both genes were found at higher levels in lymphoid tissues and the expression of DEC205 in normal birds had a characteristic distribution in the primary lymphoid organs. In spleen, DEC205 was seen on cells ideally located to trap antigen. In thymus it was found on cells thought to participate in the education of T cells, and in the bursa on cells that may be involved in presentation of antigen to B cells and regulation of B cell migration. The expression of DEC205 on cells other than antigen presenting cells (APC) is also described. Isolated splenocytes strongly expressing DEC205 but not the KUL01 antigen have morphology similar to mammalian dendritic cells and the distinct expression of DEC205 within the avian-specific Bursa of Fabricius alludes to a unique function in this organ of B cell diversification.</description><identifier>ISSN: 1932-6203</identifier><identifier>EISSN: 1932-6203</identifier><identifier>DOI: 10.1371/journal.pone.0051799</identifier><identifier>PMID: 23326318</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adaptive immunity ; Adaptive systems ; Amino Acid Sequence ; Analysis ; Animal tissues ; Animals ; Antigen presentation ; Antigen-presenting cells ; Antigens ; Antigens, CD - genetics ; Antigens, CD - metabolism ; Avian Proteins - genetics ; Avian Proteins - metabolism ; B cells ; Base Sequence ; Biology ; Birds ; Bursa of Fabricius ; Bursa of Fabricius - cytology ; Bursa of Fabricius - metabolism ; Butter ; CD83 Antigen ; Cell migration ; Cells, Cultured ; Chickens ; Chickens - genetics ; Chlorocebus aethiops ; Cloning ; Cloning, Molecular ; COS Cells ; Dendritic cells ; DNA, Complementary - chemistry ; DNA, Complementary - genetics ; Gene Expression ; Humans ; Immune response ; Immune system ; Immune System - metabolism ; Immunoglobulins ; Immunoglobulins - genetics ; Immunoglobulins - metabolism ; Immunohistochemistry ; Immunology ; Knowledge management ; Laboratories ; Lectins, C-Type - genetics ; Lectins, C-Type - metabolism ; Livestock ; Lymphocytes ; Lymphocytes B ; Lymphocytes T ; Lymphoid tissue ; Mammals ; Medicine ; Membrane Glycoproteins - genetics ; Membrane Glycoproteins - metabolism ; Microscopy, Confocal ; Minor Histocompatibility Antigens ; Molecular Sequence Data ; Monoclonal antibodies ; Organs ; Poultry ; Proteins ; Receptors, Cell Surface - genetics ; Receptors, Cell Surface - metabolism ; Reverse Transcriptase Polymerase Chain Reaction ; Sequence Analysis, DNA ; Spleen ; Splenocytes ; Structure-function relationships ; T cells ; Thymus ; Veterinary Science</subject><ispartof>PloS one, 2013-01, Vol.8 (1), p.e51799-e51799</ispartof><rights>COPYRIGHT 2013 Public Library of Science</rights><rights>2013 Staines et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License: https://creativecommons.org/licenses/by/4.0/ (the “License”), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License.</rights><rights>2013 Staines et al 2013 Staines et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c692t-70eca87eeeed16fb13c3ab93fcbbd8b7e147d5d1880d248b64e73b42a2718eba3</citedby><cites>FETCH-LOGICAL-c692t-70eca87eeeed16fb13c3ab93fcbbd8b7e147d5d1880d248b64e73b42a2718eba3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541370/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3541370/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/23326318$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>May, Robin Charles</contributor><creatorcontrib>Staines, Karen</creatorcontrib><creatorcontrib>Young, John R</creatorcontrib><creatorcontrib>Butter, Colin</creatorcontrib><title>Expression of chicken DEC205 reflects the unique structure and function of the avian immune system</title><title>PloS one</title><addtitle>PLoS One</addtitle><description>The generation of appropriate adaptive immune responses relies critically on dendritic cells, about which relatively little is known in chickens, a vital livestock species, in comparison with man and mouse. We cloned and sequenced chicken DEC205 cDNA and used this knowledge to produce quantitative PCR assays and monoclonal antibodies to study expression of DEC205 as well as CD83. The gene structure of DEC205 was identical to those of other species. Transcripts of both genes were found at higher levels in lymphoid tissues and the expression of DEC205 in normal birds had a characteristic distribution in the primary lymphoid organs. In spleen, DEC205 was seen on cells ideally located to trap antigen. In thymus it was found on cells thought to participate in the education of T cells, and in the bursa on cells that may be involved in presentation of antigen to B cells and regulation of B cell migration. The expression of DEC205 on cells other than antigen presenting cells (APC) is also described. 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genetics</subject><subject>Chlorocebus aethiops</subject><subject>Cloning</subject><subject>Cloning, Molecular</subject><subject>COS Cells</subject><subject>Dendritic cells</subject><subject>DNA, Complementary - chemistry</subject><subject>DNA, Complementary - genetics</subject><subject>Gene Expression</subject><subject>Humans</subject><subject>Immune response</subject><subject>Immune system</subject><subject>Immune System - metabolism</subject><subject>Immunoglobulins</subject><subject>Immunoglobulins - genetics</subject><subject>Immunoglobulins - metabolism</subject><subject>Immunohistochemistry</subject><subject>Immunology</subject><subject>Knowledge management</subject><subject>Laboratories</subject><subject>Lectins, C-Type - genetics</subject><subject>Lectins, C-Type - metabolism</subject><subject>Livestock</subject><subject>Lymphocytes</subject><subject>Lymphocytes B</subject><subject>Lymphocytes T</subject><subject>Lymphoid tissue</subject><subject>Mammals</subject><subject>Medicine</subject><subject>Membrane Glycoproteins - genetics</subject><subject>Membrane Glycoproteins - metabolism</subject><subject>Microscopy, Confocal</subject><subject>Minor Histocompatibility Antigens</subject><subject>Molecular Sequence Data</subject><subject>Monoclonal antibodies</subject><subject>Organs</subject><subject>Poultry</subject><subject>Proteins</subject><subject>Receptors, Cell Surface - genetics</subject><subject>Receptors, Cell Surface - metabolism</subject><subject>Reverse Transcriptase Polymerase Chain Reaction</subject><subject>Sequence Analysis, DNA</subject><subject>Spleen</subject><subject>Splenocytes</subject><subject>Structure-function relationships</subject><subject>T cells</subject><subject>Thymus</subject><subject>Veterinary Science</subject><issn>1932-6203</issn><issn>1932-6203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2013</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>GNUQQ</sourceid><sourceid>DOA</sourceid><recordid>eNqNk11v0zAUhiMEYmPwDxBEQkJw0eKv2s4N0lQKVJo0ia9by3ac1iWxi-1M27_HWbOpQbsgvohlP-977ONziuIlBHOIGfyw831wsp3vvTNzABaQVdWj4hRWGM0oAvjx0fykeBbjLkOYU_q0OEEYI4ohPy3U6nofTIzWu9I3pd5a_du48tNqicCiDKZpjU6xTFtT9s7-6U0ZU-h16oMppavLpnc6jeIBkldWutJ2Xe8yehOT6Z4XTxrZRvNi_J8VPz-vfiy_zi4uv6yX5xczTSuUZgwYLTkz-ashbRTEGktV4UYrVXPFDCSsXtSQc1AjwhUlhmFFkEQMcqMkPiteH3z3rY9iTE8UEPEKUAApycT6QNRe7sQ-2E6GG-GlFbcLPmyEDMnq1gjJkG6ahlBVQ0I4qxQmGHDJFWkI0CZ7fRyj9aoztTYuBdlOTKc7zm7Fxl8JvCD5_UA2eDcaBJ_zGpPobNSmbaUzvh_OzTBFlN-e-80_6MO3G6mNzBewrvE5rh5MxTlhHC84pEPY-QNUHrXprM611Ni8PhG8nwgyk8x12sg-RrH-_u3_2ctfU_btEbs1sk3b6Nt-qKY4BckB1MHHmEvyPskQiKEV7rIhhlYQYytk2avjB7oX3dU-_guVvgRs</recordid><startdate>20130109</startdate><enddate>20130109</enddate><creator>Staines, Karen</creator><creator>Young, John R</creator><creator>Butter, Colin</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>IOV</scope><scope>ISR</scope><scope>3V.</scope><scope>7QG</scope><scope>7QL</scope><scope>7QO</scope><scope>7RV</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TG</scope><scope>7TM</scope><scope>7U9</scope><scope>7X2</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8AO</scope><scope>8C1</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>ARAPS</scope><scope>ATCPS</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>D1I</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>H94</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>KB.</scope><scope>KB0</scope><scope>KL.</scope><scope>L6V</scope><scope>LK8</scope><scope>M0K</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>NAPCQ</scope><scope>P5Z</scope><scope>P62</scope><scope>P64</scope><scope>PATMY</scope><scope>PDBOC</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PRINS</scope><scope>PTHSS</scope><scope>PYCSY</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20130109</creationdate><title>Expression of chicken DEC205 reflects the unique structure and function of the avian immune system</title><author>Staines, Karen ; Young, John R ; Butter, Colin</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c692t-70eca87eeeed16fb13c3ab93fcbbd8b7e147d5d1880d248b64e73b42a2718eba3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2013</creationdate><topic>Adaptive immunity</topic><topic>Adaptive systems</topic><topic>Amino Acid Sequence</topic><topic>Analysis</topic><topic>Animal tissues</topic><topic>Animals</topic><topic>Antigen presentation</topic><topic>Antigen-presenting cells</topic><topic>Antigens</topic><topic>Antigens, CD - genetics</topic><topic>Antigens, CD - metabolism</topic><topic>Avian Proteins - genetics</topic><topic>Avian Proteins - metabolism</topic><topic>B cells</topic><topic>Base Sequence</topic><topic>Biology</topic><topic>Birds</topic><topic>Bursa of Fabricius</topic><topic>Bursa of Fabricius - cytology</topic><topic>Bursa of Fabricius - metabolism</topic><topic>Butter</topic><topic>CD83 Antigen</topic><topic>Cell migration</topic><topic>Cells, Cultured</topic><topic>Chickens</topic><topic>Chickens - genetics</topic><topic>Chlorocebus aethiops</topic><topic>Cloning</topic><topic>Cloning, Molecular</topic><topic>COS Cells</topic><topic>Dendritic cells</topic><topic>DNA, Complementary - chemistry</topic><topic>DNA, Complementary - genetics</topic><topic>Gene Expression</topic><topic>Humans</topic><topic>Immune response</topic><topic>Immune system</topic><topic>Immune System - metabolism</topic><topic>Immunoglobulins</topic><topic>Immunoglobulins - genetics</topic><topic>Immunoglobulins - metabolism</topic><topic>Immunohistochemistry</topic><topic>Immunology</topic><topic>Knowledge management</topic><topic>Laboratories</topic><topic>Lectins, C-Type - 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Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PloS one</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Staines, Karen</au><au>Young, John R</au><au>Butter, Colin</au><au>May, Robin Charles</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of chicken DEC205 reflects the unique structure and function of the avian immune system</atitle><jtitle>PloS one</jtitle><addtitle>PLoS One</addtitle><date>2013-01-09</date><risdate>2013</risdate><volume>8</volume><issue>1</issue><spage>e51799</spage><epage>e51799</epage><pages>e51799-e51799</pages><issn>1932-6203</issn><eissn>1932-6203</eissn><abstract>The generation of appropriate adaptive immune responses relies critically on dendritic cells, about which relatively little is known in chickens, a vital livestock species, in comparison with man and mouse. We cloned and sequenced chicken DEC205 cDNA and used this knowledge to produce quantitative PCR assays and monoclonal antibodies to study expression of DEC205 as well as CD83. The gene structure of DEC205 was identical to those of other species. Transcripts of both genes were found at higher levels in lymphoid tissues and the expression of DEC205 in normal birds had a characteristic distribution in the primary lymphoid organs. In spleen, DEC205 was seen on cells ideally located to trap antigen. In thymus it was found on cells thought to participate in the education of T cells, and in the bursa on cells that may be involved in presentation of antigen to B cells and regulation of B cell migration. The expression of DEC205 on cells other than antigen presenting cells (APC) is also described. Isolated splenocytes strongly expressing DEC205 but not the KUL01 antigen have morphology similar to mammalian dendritic cells and the distinct expression of DEC205 within the avian-specific Bursa of Fabricius alludes to a unique function in this organ of B cell diversification.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>23326318</pmid><doi>10.1371/journal.pone.0051799</doi><tpages>e51799</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 1932-6203 |
| ispartof | PloS one, 2013-01, Vol.8 (1), p.e51799-e51799 |
| issn | 1932-6203 1932-6203 |
| language | eng |
| recordid | cdi_plos_journals_1289060164 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; Public Library of Science (PLoS); PubMed Central; Free Full-Text Journals in Chemistry |
| subjects | Adaptive immunity Adaptive systems Amino Acid Sequence Analysis Animal tissues Animals Antigen presentation Antigen-presenting cells Antigens Antigens, CD - genetics Antigens, CD - metabolism Avian Proteins - genetics Avian Proteins - metabolism B cells Base Sequence Biology Birds Bursa of Fabricius Bursa of Fabricius - cytology Bursa of Fabricius - metabolism Butter CD83 Antigen Cell migration Cells, Cultured Chickens Chickens - genetics Chlorocebus aethiops Cloning Cloning, Molecular COS Cells Dendritic cells DNA, Complementary - chemistry DNA, Complementary - genetics Gene Expression Humans Immune response Immune system Immune System - metabolism Immunoglobulins Immunoglobulins - genetics Immunoglobulins - metabolism Immunohistochemistry Immunology Knowledge management Laboratories Lectins, C-Type - genetics Lectins, C-Type - metabolism Livestock Lymphocytes Lymphocytes B Lymphocytes T Lymphoid tissue Mammals Medicine Membrane Glycoproteins - genetics Membrane Glycoproteins - metabolism Microscopy, Confocal Minor Histocompatibility Antigens Molecular Sequence Data Monoclonal antibodies Organs Poultry Proteins Receptors, Cell Surface - genetics Receptors, Cell Surface - metabolism Reverse Transcriptase Polymerase Chain Reaction Sequence Analysis, DNA Spleen Splenocytes Structure-function relationships T cells Thymus Veterinary Science |
| title | Expression of chicken DEC205 reflects the unique structure and function of the avian immune system |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-19T12%3A36%3A38IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Expression%20of%20chicken%20DEC205%20reflects%20the%20unique%20structure%20and%20function%20of%20the%20avian%20immune%20system&rft.jtitle=PloS%20one&rft.au=Staines,%20Karen&rft.date=2013-01-09&rft.volume=8&rft.issue=1&rft.spage=e51799&rft.epage=e51799&rft.pages=e51799-e51799&rft.issn=1932-6203&rft.eissn=1932-6203&rft_id=info:doi/10.1371/journal.pone.0051799&rft_dat=%3Cgale_plos_%3EA478358160%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1289060164&rft_id=info:pmid/23326318&rft_galeid=A478358160&rft_doaj_id=oai_doaj_org_article_a72cfff46bd144879b34308a8b4f40ce&rfr_iscdi=true |