Identification of a novel polyomavirus from patients with acute respiratory tract infections
We report the identification of a novel polyomavirus present in respiratory secretions from human patients with symptoms of acute respiratory tract infection. The virus was initially detected in a nasopharyngeal aspirate from a 3-year-old child from Australia diagnosed with pneumonia. A random libra...
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| Veröffentlicht in: | PLoS pathogens 2007-05, Vol.3 (5), p.e64-e64 |
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| creator | Gaynor, Anne M Nissen, Michael D Whiley, David M Mackay, Ian M Lambert, Stephen B Wu, Guang Brennan, Daniel C Storch, Gregory A Sloots, Theo P Wang, David |
| description | We report the identification of a novel polyomavirus present in respiratory secretions from human patients with symptoms of acute respiratory tract infection. The virus was initially detected in a nasopharyngeal aspirate from a 3-year-old child from Australia diagnosed with pneumonia. A random library was generated from nucleic acids extracted from the nasopharyngeal aspirate and analyzed by high throughput DNA sequencing. Multiple DNA fragments were cloned that possessed limited homology to known polyomaviruses. We subsequently sequenced the entire virus genome of 5,229 bp, henceforth referred to as WU virus, and found it to have genomic features characteristic of the family Polyomaviridae. The genome was predicted to encode small T antigen, large T antigen, and three capsid proteins: VP1, VP2, and VP3. Phylogenetic analysis clearly revealed that the WU virus was divergent from all known polyomaviruses. Screening of 2,135 patients with acute respiratory tract infections in Brisbane, Queensland, Australia, and St. Louis, Missouri, United States, using WU virus-specific PCR primers resulted in the detection of 43 additional specimens that contained WU virus. The presence of multiple instances of the virus in two continents suggests that this virus is geographically widespread in the human population and raises the possibility that the WU virus may be a human pathogen. |
| doi_str_mv | 10.1371/journal.ppat.0030064 |
| format | Article |
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The virus was initially detected in a nasopharyngeal aspirate from a 3-year-old child from Australia diagnosed with pneumonia. A random library was generated from nucleic acids extracted from the nasopharyngeal aspirate and analyzed by high throughput DNA sequencing. Multiple DNA fragments were cloned that possessed limited homology to known polyomaviruses. We subsequently sequenced the entire virus genome of 5,229 bp, henceforth referred to as WU virus, and found it to have genomic features characteristic of the family Polyomaviridae. The genome was predicted to encode small T antigen, large T antigen, and three capsid proteins: VP1, VP2, and VP3. Phylogenetic analysis clearly revealed that the WU virus was divergent from all known polyomaviruses. Screening of 2,135 patients with acute respiratory tract infections in Brisbane, Queensland, Australia, and St. Louis, Missouri, United States, using WU virus-specific PCR primers resulted in the detection of 43 additional specimens that contained WU virus. The presence of multiple instances of the virus in two continents suggests that this virus is geographically widespread in the human population and raises the possibility that the WU virus may be a human pathogen.</description><identifier>ISSN: 1553-7374</identifier><identifier>ISSN: 1553-7366</identifier><identifier>EISSN: 1553-7374</identifier><identifier>DOI: 10.1371/journal.ppat.0030064</identifier><identifier>PMID: 17480120</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Acute Disease ; Amino acids ; Base Sequence ; Binding sites ; Deoxyribonucleic acid ; DNA ; DNA Probes ; Genetic aspects ; Genome, Viral ; Genomes ; Global Health ; Homo (Human) ; Humans ; Infections ; Infectious Diseases ; Microbiology ; Molecular Sequence Data ; Polymerase Chain Reaction ; Polyomavirus - genetics ; Polyomavirus - isolation & purification ; Proteins ; Respiratory tract infections ; Respiratory Tract Infections - virology ; Viral infections ; Viral Proteins ; Virology ; Viruses</subject><ispartof>PLoS pathogens, 2007-05, Vol.3 (5), p.e64-e64</ispartof><rights>COPYRIGHT 2007 Public Library of Science</rights><rights>2007 Gaynor et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Gaynor AM, Nissen MD, Whiley DM, Mackay IM, Lambert SB, et al. (2007) Identification of a Novel Polyomavirus from Patients with Acute Respiratory Tract Infections. PLoS Pathog 3(5): e64. doi:10.1371/journal.ppat.0030064</rights><rights>2007 Gaynor et al. 2007</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c659t-4cbc2e8fe3da183cec5946a76c46ed1ec072b19fa0c4733de130cfeac956405b3</citedby><cites>FETCH-LOGICAL-c659t-4cbc2e8fe3da183cec5946a76c46ed1ec072b19fa0c4733de130cfeac956405b3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1864993/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1864993/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23847,27903,27904,53769,53771,79346,79347</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/17480120$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Ganem, Donald E</contributor><creatorcontrib>Gaynor, Anne M</creatorcontrib><creatorcontrib>Nissen, Michael D</creatorcontrib><creatorcontrib>Whiley, David M</creatorcontrib><creatorcontrib>Mackay, Ian M</creatorcontrib><creatorcontrib>Lambert, Stephen B</creatorcontrib><creatorcontrib>Wu, Guang</creatorcontrib><creatorcontrib>Brennan, Daniel C</creatorcontrib><creatorcontrib>Storch, Gregory A</creatorcontrib><creatorcontrib>Sloots, Theo P</creatorcontrib><creatorcontrib>Wang, David</creatorcontrib><title>Identification of a novel polyomavirus from patients with acute respiratory tract infections</title><title>PLoS pathogens</title><addtitle>PLoS Pathog</addtitle><description>We report the identification of a novel polyomavirus present in respiratory secretions from human patients with symptoms of acute respiratory tract infection. 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Screening of 2,135 patients with acute respiratory tract infections in Brisbane, Queensland, Australia, and St. Louis, Missouri, United States, using WU virus-specific PCR primers resulted in the detection of 43 additional specimens that contained WU virus. The presence of multiple instances of the virus in two continents suggests that this virus is geographically widespread in the human population and raises the possibility that the WU virus may be a human pathogen.</description><subject>Acute Disease</subject><subject>Amino acids</subject><subject>Base Sequence</subject><subject>Binding sites</subject><subject>Deoxyribonucleic acid</subject><subject>DNA</subject><subject>DNA Probes</subject><subject>Genetic aspects</subject><subject>Genome, Viral</subject><subject>Genomes</subject><subject>Global Health</subject><subject>Homo (Human)</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious Diseases</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Polyomavirus - genetics</subject><subject>Polyomavirus - isolation & purification</subject><subject>Proteins</subject><subject>Respiratory tract infections</subject><subject>Respiratory Tract Infections - 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Screening of 2,135 patients with acute respiratory tract infections in Brisbane, Queensland, Australia, and St. Louis, Missouri, United States, using WU virus-specific PCR primers resulted in the detection of 43 additional specimens that contained WU virus. The presence of multiple instances of the virus in two continents suggests that this virus is geographically widespread in the human population and raises the possibility that the WU virus may be a human pathogen.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>17480120</pmid><doi>10.1371/journal.ppat.0030064</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acute Disease Amino acids Base Sequence Binding sites Deoxyribonucleic acid DNA DNA Probes Genetic aspects Genome, Viral Genomes Global Health Homo (Human) Humans Infections Infectious Diseases Microbiology Molecular Sequence Data Polymerase Chain Reaction Polyomavirus - genetics Polyomavirus - isolation & purification Proteins Respiratory tract infections Respiratory Tract Infections - virology Viral infections Viral Proteins Virology Viruses |
| title | Identification of a novel polyomavirus from patients with acute respiratory tract infections |
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