Diagnostic accuracy of the Leishmania OligoC-TesT and NASBA-Oligochromatography for diagnosis of leishmaniasis in Sudan
The Leishmania OligoC-TesT and NASBA-Oligochromatography (OC) were recently developed for simplified and standardised molecular detection of Leishmania parasites in clinical specimens. We here present the phase II evaluation of both tests for diagnosis of visceral leishmaniasis (VL), cutaneous leish...
Gespeichert in:
| Veröffentlicht in: | PLoS neglected tropical diseases 2010-08, Vol.4 (8), p.e776-e776 |
|---|---|
| Hauptverfasser: | , , , , , , , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e776 |
|---|---|
| container_issue | 8 |
| container_start_page | e776 |
| container_title | PLoS neglected tropical diseases |
| container_volume | 4 |
| creator | Saad, Alfarazdeg A Ahmed, Nuha G Osman, Osman S Al-Basheer, Ahmed Almustafa Hamad, Awad Deborggraeve, Stijn Büscher, Philippe Schoone, Gerard J Schallig, Henk D Laurent, Thierry Haleem, Ahmed Osman, Omran F Eltom, Ahmed Mohamedain Elbashir, Mustafa I El-Safi, Sayda |
| description | The Leishmania OligoC-TesT and NASBA-Oligochromatography (OC) were recently developed for simplified and standardised molecular detection of Leishmania parasites in clinical specimens. We here present the phase II evaluation of both tests for diagnosis of visceral leishmaniasis (VL), cutaneous leishmaniasis (CL) and post kala-azar dermal leishmaniasis (PKDL) in Sudan.
The diagnostic accuracy of the tests was evaluated on 90 confirmed and 90 suspected VL cases, 7 confirmed and 8 suspected CL cases, 2 confirmed PKDL cases and 50 healthy endemic controls from Gedarif state and Khartoum state in Sudan.
The OligoC-TesT as well as the NASBA-OC showed a sensitivity of 96.8% (95% CI: 83.8%-99.4%) on lymph node aspirates and of 96.2% (95% CI: 89.4%-98.7%) on blood from the confirmed VL cases. The sensitivity on bone marrow was 96.9% (95% CI: 89.3%-99.1%) and 95.3% (95% CI: 87.1%-98.4%) for the OligoC-TesT and NASBA-OC, respectively. All confirmed CL and PKDL cases were positive with both tests. On the suspected VL cases, we observed a positive OligoC-TesT and NASBA-OC result in 37.1% (95% CI: 23.2%-53.7%) and 34.3% (95% CI: 20.8%-50.9%) on lymph, in 72.7% (95% CI: 55.8%-84.9%) and 63.6% (95% CI: 46.6%-77.8%) on bone marrow and in 76.9% (95% CI: 49.7%-91.8%) and 69.2% (95% CI: 42.4%-87.3%) on blood. Seven out of 8 CL suspected cases were positive with both tests. The specificity on the healthy endemic controls was 90% (95% CI: 78.6%-95.7%) for the OligoC-TesT and 100% (95% CI: 92.9%-100.0%) for the NASBA-OC test.
Both tests showed high sensitivity on lymph, blood and tissue scrapings for diagnosis of VL, CL and PKDL in Sudan, but the specificity for clinical VL was significantly higher with NASBA-OC. |
| doi_str_mv | 10.1371/journal.pntd.0000776 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1288113988</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_b322c31d3d834f9db74b0f53d060a5ee</doaj_id><sourcerecordid>748934394</sourcerecordid><originalsourceid>FETCH-LOGICAL-c557t-eceb17d0c35e2a455c86759340f0bb3efd67b40f253d903d996c5e5c962360973</originalsourceid><addsrcrecordid>eNqFkl1v2yAUhq1p09p1-wfThrSLXjnjwxi4mZSm-6gUrRfNrhEGbBPZJgN7U_79SONG7TRpSAg4vOcBDm-WvUVwgQhDH7d-CoPqFrthNAuYGmPls-wcCUJzzAh9_mh-lr2KcQshFZSjl9kZhiUXHOPz7Pe1U83g4-g0UFpPQek98DUYWwvW1sW2V4NT4LZzjV_lGxs3QA0GfF_eXS3z-6hug-_V6Jugdu0e1D4Ac2S6eCB1J8oh4AZwNxk1vM5e1KqL9s08XmQ_vnzerL7l69uvN6vlOteUsjG32laIGagJtVgVlGpeMipIAWtYVcTWpmRVWmBKjICpi1JTS7UoMSmhYOQie3_k7jof5VyzKBHmHCEiOE-Km6PCeLWVu-B6FfbSKyfvAz40UoVUns7KimCsCTLEcFLUwlSsqGCdjoYlVNTaxPo0nzZVvTXaDmNQ3RPo053BtbLxvyQWqGAcJ8DlDAj-52TjKHsXte06NVg_RckoTZ-PWfl_ZcFTmYgokvLDX8p_l6E4qnTwMQZbn26NoDwY7iFLHgwnZ8OltHePX3xKenAY-QMFRNSh</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1288113988</pqid></control><display><type>article</type><title>Diagnostic accuracy of the Leishmania OligoC-TesT and NASBA-Oligochromatography for diagnosis of leishmaniasis in Sudan</title><source>PubMed (Medline)</source><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>EZB-FREE-00999 freely available EZB journals</source><source>PubMed Central Open Access</source><source>Public Library of Science (PLoS)</source><creator>Saad, Alfarazdeg A ; Ahmed, Nuha G ; Osman, Osman S ; Al-Basheer, Ahmed Almustafa ; Hamad, Awad ; Deborggraeve, Stijn ; Büscher, Philippe ; Schoone, Gerard J ; Schallig, Henk D ; Laurent, Thierry ; Haleem, Ahmed ; Osman, Omran F ; Eltom, Ahmed Mohamedain ; Elbashir, Mustafa I ; El-Safi, Sayda</creator><contributor>Bates, Paul Andrew</contributor><creatorcontrib>Saad, Alfarazdeg A ; Ahmed, Nuha G ; Osman, Osman S ; Al-Basheer, Ahmed Almustafa ; Hamad, Awad ; Deborggraeve, Stijn ; Büscher, Philippe ; Schoone, Gerard J ; Schallig, Henk D ; Laurent, Thierry ; Haleem, Ahmed ; Osman, Omran F ; Eltom, Ahmed Mohamedain ; Elbashir, Mustafa I ; El-Safi, Sayda ; Bates, Paul Andrew</creatorcontrib><description>The Leishmania OligoC-TesT and NASBA-Oligochromatography (OC) were recently developed for simplified and standardised molecular detection of Leishmania parasites in clinical specimens. We here present the phase II evaluation of both tests for diagnosis of visceral leishmaniasis (VL), cutaneous leishmaniasis (CL) and post kala-azar dermal leishmaniasis (PKDL) in Sudan.
The diagnostic accuracy of the tests was evaluated on 90 confirmed and 90 suspected VL cases, 7 confirmed and 8 suspected CL cases, 2 confirmed PKDL cases and 50 healthy endemic controls from Gedarif state and Khartoum state in Sudan.
The OligoC-TesT as well as the NASBA-OC showed a sensitivity of 96.8% (95% CI: 83.8%-99.4%) on lymph node aspirates and of 96.2% (95% CI: 89.4%-98.7%) on blood from the confirmed VL cases. The sensitivity on bone marrow was 96.9% (95% CI: 89.3%-99.1%) and 95.3% (95% CI: 87.1%-98.4%) for the OligoC-TesT and NASBA-OC, respectively. All confirmed CL and PKDL cases were positive with both tests. On the suspected VL cases, we observed a positive OligoC-TesT and NASBA-OC result in 37.1% (95% CI: 23.2%-53.7%) and 34.3% (95% CI: 20.8%-50.9%) on lymph, in 72.7% (95% CI: 55.8%-84.9%) and 63.6% (95% CI: 46.6%-77.8%) on bone marrow and in 76.9% (95% CI: 49.7%-91.8%) and 69.2% (95% CI: 42.4%-87.3%) on blood. Seven out of 8 CL suspected cases were positive with both tests. The specificity on the healthy endemic controls was 90% (95% CI: 78.6%-95.7%) for the OligoC-TesT and 100% (95% CI: 92.9%-100.0%) for the NASBA-OC test.
Both tests showed high sensitivity on lymph, blood and tissue scrapings for diagnosis of VL, CL and PKDL in Sudan, but the specificity for clinical VL was significantly higher with NASBA-OC.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0000776</identifier><identifier>PMID: 20689822</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Accuracy ; Animals ; Blood ; Blood - parasitology ; Bone marrow ; Bone Marrow - parasitology ; Disease ; Health facilities ; Humans ; Infectious Diseases/Protozoal Infections ; Leishmania ; Leishmania - isolation & purification ; Leishmaniasis - diagnosis ; Lymph Nodes - parasitology ; Molecular Diagnostic Techniques - methods ; Nucleic Acid Amplification Techniques - methods ; Parasites ; Parasitic diseases ; Parasitology - methods ; Polymerase chain reaction ; Primary care ; Reagent Kits, Diagnostic ; Sensitivity and Specificity ; Sudan ; Tropical diseases ; Vector-borne diseases</subject><ispartof>PLoS neglected tropical diseases, 2010-08, Vol.4 (8), p.e776-e776</ispartof><rights>2010 Saad et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Saad AA, Ahmed NG, Osman OS, Al-Basheer AA, Hamad A, et al. (2010) Diagnostic Accuracy of the Leishmania OligoC-TesT and NASBA-Oligochromatography for Diagnosis of Leishmaniasis in Sudan. PLoS Negl Trop Dis 4(8): e776. doi:10.1371/journal.pntd.0000776</rights><rights>Saad et al. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-eceb17d0c35e2a455c86759340f0bb3efd67b40f253d903d996c5e5c962360973</citedby><cites>FETCH-LOGICAL-c557t-eceb17d0c35e2a455c86759340f0bb3efd67b40f253d903d996c5e5c962360973</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914782/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2914782/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793,79600,79601</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20689822$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Bates, Paul Andrew</contributor><creatorcontrib>Saad, Alfarazdeg A</creatorcontrib><creatorcontrib>Ahmed, Nuha G</creatorcontrib><creatorcontrib>Osman, Osman S</creatorcontrib><creatorcontrib>Al-Basheer, Ahmed Almustafa</creatorcontrib><creatorcontrib>Hamad, Awad</creatorcontrib><creatorcontrib>Deborggraeve, Stijn</creatorcontrib><creatorcontrib>Büscher, Philippe</creatorcontrib><creatorcontrib>Schoone, Gerard J</creatorcontrib><creatorcontrib>Schallig, Henk D</creatorcontrib><creatorcontrib>Laurent, Thierry</creatorcontrib><creatorcontrib>Haleem, Ahmed</creatorcontrib><creatorcontrib>Osman, Omran F</creatorcontrib><creatorcontrib>Eltom, Ahmed Mohamedain</creatorcontrib><creatorcontrib>Elbashir, Mustafa I</creatorcontrib><creatorcontrib>El-Safi, Sayda</creatorcontrib><title>Diagnostic accuracy of the Leishmania OligoC-TesT and NASBA-Oligochromatography for diagnosis of leishmaniasis in Sudan</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>The Leishmania OligoC-TesT and NASBA-Oligochromatography (OC) were recently developed for simplified and standardised molecular detection of Leishmania parasites in clinical specimens. We here present the phase II evaluation of both tests for diagnosis of visceral leishmaniasis (VL), cutaneous leishmaniasis (CL) and post kala-azar dermal leishmaniasis (PKDL) in Sudan.
The diagnostic accuracy of the tests was evaluated on 90 confirmed and 90 suspected VL cases, 7 confirmed and 8 suspected CL cases, 2 confirmed PKDL cases and 50 healthy endemic controls from Gedarif state and Khartoum state in Sudan.
The OligoC-TesT as well as the NASBA-OC showed a sensitivity of 96.8% (95% CI: 83.8%-99.4%) on lymph node aspirates and of 96.2% (95% CI: 89.4%-98.7%) on blood from the confirmed VL cases. The sensitivity on bone marrow was 96.9% (95% CI: 89.3%-99.1%) and 95.3% (95% CI: 87.1%-98.4%) for the OligoC-TesT and NASBA-OC, respectively. All confirmed CL and PKDL cases were positive with both tests. On the suspected VL cases, we observed a positive OligoC-TesT and NASBA-OC result in 37.1% (95% CI: 23.2%-53.7%) and 34.3% (95% CI: 20.8%-50.9%) on lymph, in 72.7% (95% CI: 55.8%-84.9%) and 63.6% (95% CI: 46.6%-77.8%) on bone marrow and in 76.9% (95% CI: 49.7%-91.8%) and 69.2% (95% CI: 42.4%-87.3%) on blood. Seven out of 8 CL suspected cases were positive with both tests. The specificity on the healthy endemic controls was 90% (95% CI: 78.6%-95.7%) for the OligoC-TesT and 100% (95% CI: 92.9%-100.0%) for the NASBA-OC test.
Both tests showed high sensitivity on lymph, blood and tissue scrapings for diagnosis of VL, CL and PKDL in Sudan, but the specificity for clinical VL was significantly higher with NASBA-OC.</description><subject>Accuracy</subject><subject>Animals</subject><subject>Blood</subject><subject>Blood - parasitology</subject><subject>Bone marrow</subject><subject>Bone Marrow - parasitology</subject><subject>Disease</subject><subject>Health facilities</subject><subject>Humans</subject><subject>Infectious Diseases/Protozoal Infections</subject><subject>Leishmania</subject><subject>Leishmania - isolation & purification</subject><subject>Leishmaniasis - diagnosis</subject><subject>Lymph Nodes - parasitology</subject><subject>Molecular Diagnostic Techniques - methods</subject><subject>Nucleic Acid Amplification Techniques - methods</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Parasitology - methods</subject><subject>Polymerase chain reaction</subject><subject>Primary care</subject><subject>Reagent Kits, Diagnostic</subject><subject>Sensitivity and Specificity</subject><subject>Sudan</subject><subject>Tropical diseases</subject><subject>Vector-borne diseases</subject><issn>1935-2735</issn><issn>1935-2727</issn><issn>1935-2735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNqFkl1v2yAUhq1p09p1-wfThrSLXjnjwxi4mZSm-6gUrRfNrhEGbBPZJgN7U_79SONG7TRpSAg4vOcBDm-WvUVwgQhDH7d-CoPqFrthNAuYGmPls-wcCUJzzAh9_mh-lr2KcQshFZSjl9kZhiUXHOPz7Pe1U83g4-g0UFpPQek98DUYWwvW1sW2V4NT4LZzjV_lGxs3QA0GfF_eXS3z-6hug-_V6Jugdu0e1D4Ac2S6eCB1J8oh4AZwNxk1vM5e1KqL9s08XmQ_vnzerL7l69uvN6vlOteUsjG32laIGagJtVgVlGpeMipIAWtYVcTWpmRVWmBKjICpi1JTS7UoMSmhYOQie3_k7jof5VyzKBHmHCEiOE-Km6PCeLWVu-B6FfbSKyfvAz40UoVUns7KimCsCTLEcFLUwlSsqGCdjoYlVNTaxPo0nzZVvTXaDmNQ3RPo053BtbLxvyQWqGAcJ8DlDAj-52TjKHsXte06NVg_RckoTZ-PWfl_ZcFTmYgokvLDX8p_l6E4qnTwMQZbn26NoDwY7iFLHgwnZ8OltHePX3xKenAY-QMFRNSh</recordid><startdate>20100803</startdate><enddate>20100803</enddate><creator>Saad, Alfarazdeg A</creator><creator>Ahmed, Nuha G</creator><creator>Osman, Osman S</creator><creator>Al-Basheer, Ahmed Almustafa</creator><creator>Hamad, Awad</creator><creator>Deborggraeve, Stijn</creator><creator>Büscher, Philippe</creator><creator>Schoone, Gerard J</creator><creator>Schallig, Henk D</creator><creator>Laurent, Thierry</creator><creator>Haleem, Ahmed</creator><creator>Osman, Omran F</creator><creator>Eltom, Ahmed Mohamedain</creator><creator>Elbashir, Mustafa I</creator><creator>El-Safi, Sayda</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7T2</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20100803</creationdate><title>Diagnostic accuracy of the Leishmania OligoC-TesT and NASBA-Oligochromatography for diagnosis of leishmaniasis in Sudan</title><author>Saad, Alfarazdeg A ; Ahmed, Nuha G ; Osman, Osman S ; Al-Basheer, Ahmed Almustafa ; Hamad, Awad ; Deborggraeve, Stijn ; Büscher, Philippe ; Schoone, Gerard J ; Schallig, Henk D ; Laurent, Thierry ; Haleem, Ahmed ; Osman, Omran F ; Eltom, Ahmed Mohamedain ; Elbashir, Mustafa I ; El-Safi, Sayda</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-eceb17d0c35e2a455c86759340f0bb3efd67b40f253d903d996c5e5c962360973</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Accuracy</topic><topic>Animals</topic><topic>Blood</topic><topic>Blood - parasitology</topic><topic>Bone marrow</topic><topic>Bone Marrow - parasitology</topic><topic>Disease</topic><topic>Health facilities</topic><topic>Humans</topic><topic>Infectious Diseases/Protozoal Infections</topic><topic>Leishmania</topic><topic>Leishmania - isolation & purification</topic><topic>Leishmaniasis - diagnosis</topic><topic>Lymph Nodes - parasitology</topic><topic>Molecular Diagnostic Techniques - methods</topic><topic>Nucleic Acid Amplification Techniques - methods</topic><topic>Parasites</topic><topic>Parasitic diseases</topic><topic>Parasitology - methods</topic><topic>Polymerase chain reaction</topic><topic>Primary care</topic><topic>Reagent Kits, Diagnostic</topic><topic>Sensitivity and Specificity</topic><topic>Sudan</topic><topic>Tropical diseases</topic><topic>Vector-borne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Saad, Alfarazdeg A</creatorcontrib><creatorcontrib>Ahmed, Nuha G</creatorcontrib><creatorcontrib>Osman, Osman S</creatorcontrib><creatorcontrib>Al-Basheer, Ahmed Almustafa</creatorcontrib><creatorcontrib>Hamad, Awad</creatorcontrib><creatorcontrib>Deborggraeve, Stijn</creatorcontrib><creatorcontrib>Büscher, Philippe</creatorcontrib><creatorcontrib>Schoone, Gerard J</creatorcontrib><creatorcontrib>Schallig, Henk D</creatorcontrib><creatorcontrib>Laurent, Thierry</creatorcontrib><creatorcontrib>Haleem, Ahmed</creatorcontrib><creatorcontrib>Osman, Omran F</creatorcontrib><creatorcontrib>Eltom, Ahmed Mohamedain</creatorcontrib><creatorcontrib>Elbashir, Mustafa I</creatorcontrib><creatorcontrib>El-Safi, Sayda</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>ProQuest Health and Medical</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PLoS neglected tropical diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Saad, Alfarazdeg A</au><au>Ahmed, Nuha G</au><au>Osman, Osman S</au><au>Al-Basheer, Ahmed Almustafa</au><au>Hamad, Awad</au><au>Deborggraeve, Stijn</au><au>Büscher, Philippe</au><au>Schoone, Gerard J</au><au>Schallig, Henk D</au><au>Laurent, Thierry</au><au>Haleem, Ahmed</au><au>Osman, Omran F</au><au>Eltom, Ahmed Mohamedain</au><au>Elbashir, Mustafa I</au><au>El-Safi, Sayda</au><au>Bates, Paul Andrew</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Diagnostic accuracy of the Leishmania OligoC-TesT and NASBA-Oligochromatography for diagnosis of leishmaniasis in Sudan</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2010-08-03</date><risdate>2010</risdate><volume>4</volume><issue>8</issue><spage>e776</spage><epage>e776</epage><pages>e776-e776</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>The Leishmania OligoC-TesT and NASBA-Oligochromatography (OC) were recently developed for simplified and standardised molecular detection of Leishmania parasites in clinical specimens. We here present the phase II evaluation of both tests for diagnosis of visceral leishmaniasis (VL), cutaneous leishmaniasis (CL) and post kala-azar dermal leishmaniasis (PKDL) in Sudan.
The diagnostic accuracy of the tests was evaluated on 90 confirmed and 90 suspected VL cases, 7 confirmed and 8 suspected CL cases, 2 confirmed PKDL cases and 50 healthy endemic controls from Gedarif state and Khartoum state in Sudan.
The OligoC-TesT as well as the NASBA-OC showed a sensitivity of 96.8% (95% CI: 83.8%-99.4%) on lymph node aspirates and of 96.2% (95% CI: 89.4%-98.7%) on blood from the confirmed VL cases. The sensitivity on bone marrow was 96.9% (95% CI: 89.3%-99.1%) and 95.3% (95% CI: 87.1%-98.4%) for the OligoC-TesT and NASBA-OC, respectively. All confirmed CL and PKDL cases were positive with both tests. On the suspected VL cases, we observed a positive OligoC-TesT and NASBA-OC result in 37.1% (95% CI: 23.2%-53.7%) and 34.3% (95% CI: 20.8%-50.9%) on lymph, in 72.7% (95% CI: 55.8%-84.9%) and 63.6% (95% CI: 46.6%-77.8%) on bone marrow and in 76.9% (95% CI: 49.7%-91.8%) and 69.2% (95% CI: 42.4%-87.3%) on blood. Seven out of 8 CL suspected cases were positive with both tests. The specificity on the healthy endemic controls was 90% (95% CI: 78.6%-95.7%) for the OligoC-TesT and 100% (95% CI: 92.9%-100.0%) for the NASBA-OC test.
Both tests showed high sensitivity on lymph, blood and tissue scrapings for diagnosis of VL, CL and PKDL in Sudan, but the specificity for clinical VL was significantly higher with NASBA-OC.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>20689822</pmid><doi>10.1371/journal.pntd.0000776</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1935-2735 |
| ispartof | PLoS neglected tropical diseases, 2010-08, Vol.4 (8), p.e776-e776 |
| issn | 1935-2735 1935-2727 1935-2735 |
| language | eng |
| recordid | cdi_plos_journals_1288113988 |
| source | PubMed (Medline); MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central Open Access; Public Library of Science (PLoS) |
| subjects | Accuracy Animals Blood Blood - parasitology Bone marrow Bone Marrow - parasitology Disease Health facilities Humans Infectious Diseases/Protozoal Infections Leishmania Leishmania - isolation & purification Leishmaniasis - diagnosis Lymph Nodes - parasitology Molecular Diagnostic Techniques - methods Nucleic Acid Amplification Techniques - methods Parasites Parasitic diseases Parasitology - methods Polymerase chain reaction Primary care Reagent Kits, Diagnostic Sensitivity and Specificity Sudan Tropical diseases Vector-borne diseases |
| title | Diagnostic accuracy of the Leishmania OligoC-TesT and NASBA-Oligochromatography for diagnosis of leishmaniasis in Sudan |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-25T14%3A40%3A49IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Diagnostic%20accuracy%20of%20the%20Leishmania%20OligoC-TesT%20and%20NASBA-Oligochromatography%20for%20diagnosis%20of%20leishmaniasis%20in%20Sudan&rft.jtitle=PLoS%20neglected%20tropical%20diseases&rft.au=Saad,%20Alfarazdeg%20A&rft.date=2010-08-03&rft.volume=4&rft.issue=8&rft.spage=e776&rft.epage=e776&rft.pages=e776-e776&rft.issn=1935-2735&rft.eissn=1935-2735&rft_id=info:doi/10.1371/journal.pntd.0000776&rft_dat=%3Cproquest_plos_%3E748934394%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1288113988&rft_id=info:pmid/20689822&rft_doaj_id=oai_doaj_org_article_b322c31d3d834f9db74b0f53d060a5ee&rfr_iscdi=true |