Molecular epidemiology of cross-species Giardia duodenalis transmission in western Uganda
Giardia duodenalis is prevalent in tropical settings where diverse opportunities exist for transmission between people and animals. We conducted a cross-sectional study of G. duodenalis in people, livestock, and wild primates near Kibale National Park, Uganda, where human-livestock-wildlife interact...
Gespeichert in:
| Veröffentlicht in: | PLoS neglected tropical diseases 2010-05, Vol.4 (5), p.e683-e683 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | e683 |
|---|---|
| container_issue | 5 |
| container_start_page | e683 |
| container_title | PLoS neglected tropical diseases |
| container_volume | 4 |
| creator | Johnston, Amanda R Gillespie, Thomas R Rwego, Innocent B McLachlan, Traci L Tranby Kent, Angela D Goldberg, Tony L |
| description | Giardia duodenalis is prevalent in tropical settings where diverse opportunities exist for transmission between people and animals. We conducted a cross-sectional study of G. duodenalis in people, livestock, and wild primates near Kibale National Park, Uganda, where human-livestock-wildlife interaction is high due to habitat disturbance. Our goal was to infer the cross-species transmission potential of G. duodenalis using molecular methods and to investigate clinical consequences of infection.
Real-time PCR on DNA extracted from fecal samples revealed a combined prevalence of G. duodenalis in people from three villages of 44/108 (40.7%), with prevalence reaching 67.5% in one village. Prevalence rates in livestock and primates were 12.4% and 11.1%, respectively. Age was associated with G. duodenalis infection in people (higher prevalence in individuals |
| doi_str_mv | 10.1371/journal.pntd.0000683 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_plos_</sourceid><recordid>TN_cdi_plos_journals_1288111306</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><doaj_id>oai_doaj_org_article_7f29ab759c574c2a867b72c727c71ca2</doaj_id><sourcerecordid>733548975</sourcerecordid><originalsourceid>FETCH-LOGICAL-c557t-24cbfa0f2c972be9f9669407a47f41282306f2270a84e4733ae82fd5a08164dc3</originalsourceid><addsrcrecordid>eNqFUk1rFTEUHUSxtfoPRAdcuJpnPieTjSBFa6Hixi5chTv5eOaRl4zJjNJ_b9o3La0IZpOQe865uSenaV5itMFU4He7tOQIYTPF2WxQXf1AHzXHWFLeEUH543vno-ZZKTuEuOQDftocEcQGziQ7br5_ScHqJUBu7eSN3fsU0vaqTa7VOZXSlclqb0t75iEbD61ZkrG1ry_tnCGWvS_Fp9j62P62ZbY5tpdbiAaeN08chGJfrPtJc_np47fTz93F17Pz0w8XneZczB1henSAHNFSkNFKJ_teMiSACccwGQhFvSNEIBiYZYJSsANxhgMacM-MpifN64PuFFJRqytFVeqAMa7sijg_IEyCnZqy30O-Ugm8urlIeasgz14Hq4QjEkbBpeaCaQJDL0ZBtCBCC6yBVK33a7dl3FujbawuhAeiDyvR_1Db9EuRKiUZqwJvV4Gcfi7VMVUd1DYEiDYtRQnWU8QZp_9HUsrZIAWvyDd_If9tAzugbj42W3f3aozUdaRuWeo6UmqNVKW9uj_xHek2Q_QPaVzKQA</addsrcrecordid><sourcetype>Open Website</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1288111306</pqid></control><display><type>article</type><title>Molecular epidemiology of cross-species Giardia duodenalis transmission in western Uganda</title><source>MEDLINE</source><source>DOAJ Directory of Open Access Journals</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><source>PubMed Central Open Access</source><source>Public Library of Science (PLoS)</source><creator>Johnston, Amanda R ; Gillespie, Thomas R ; Rwego, Innocent B ; McLachlan, Traci L Tranby ; Kent, Angela D ; Goldberg, Tony L</creator><creatorcontrib>Johnston, Amanda R ; Gillespie, Thomas R ; Rwego, Innocent B ; McLachlan, Traci L Tranby ; Kent, Angela D ; Goldberg, Tony L</creatorcontrib><description>Giardia duodenalis is prevalent in tropical settings where diverse opportunities exist for transmission between people and animals. We conducted a cross-sectional study of G. duodenalis in people, livestock, and wild primates near Kibale National Park, Uganda, where human-livestock-wildlife interaction is high due to habitat disturbance. Our goal was to infer the cross-species transmission potential of G. duodenalis using molecular methods and to investigate clinical consequences of infection.
Real-time PCR on DNA extracted from fecal samples revealed a combined prevalence of G. duodenalis in people from three villages of 44/108 (40.7%), with prevalence reaching 67.5% in one village. Prevalence rates in livestock and primates were 12.4% and 11.1%, respectively. Age was associated with G. duodenalis infection in people (higher prevalence in individuals <or=15 years) and livestock (higher prevalence in subadult versus adult animals), but other potential risk factors in people (gender, contact with domestic animals, working in fields, working in forests, source of drinking water, and medication use) were not. G. duodenalis infection was not associated with gastrointestinal symptoms in people, nor was clinical disease noted in livestock or primates. Sequence analysis of four G. duodenalis genes identified assemblage AII in humans, assemblage BIV in humans and endangered red colobus monkeys, and assemblage E in livestock and red colobus, representing the first documentation of assemblage E in a non-human primate. In addition, genetic relationships within the BIV assemblage revealed sub-clades of identical G. duodenalis sequences from humans and red colobus.
Our finding of G. duodenalis in people and primates (assemblage BIV) and livestock and primates (assemblage E) underscores that cross-species transmission of multiple G. duodenalis assemblages may occur in locations such as western Uganda where people, livestock, and primates overlap in their use of habitat. Our data also demonstrate a high but locally variable prevalence of G. duodenalis in people from western Uganda, but little evidence of associated clinical disease. Reverse zoonotic G. duodenalis transmission may be particularly frequent in tropical settings where anthropogenic habitat disturbance forces people and livestock to interact at high rates with wildlife, and this could have negative consequences for wildlife conservation.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0000683</identifier><identifier>PMID: 20485494</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Aged ; Animal diseases ; Animals ; Animals, Domestic - parasitology ; Anthropogenic factors ; Cattle ; Child ; Child, Preschool ; Cluster Analysis ; Colobus ; Cross-Sectional Studies ; Disease transmission ; DNA, Protozoan - chemistry ; DNA, Protozoan - genetics ; Domestic animals ; Drinking water ; Ecology ; Environmental protection ; Epidemiology ; Feces - parasitology ; Female ; Genotype ; Giardia duodenalis ; Giardia lamblia - classification ; Giardia lamblia - genetics ; Giardia lamblia - isolation & purification ; Giardiasis - epidemiology ; Giardiasis - parasitology ; Giardiasis - transmission ; Giardiasis - veterinary ; Habitat utilization ; Habitats ; Haplorhini - parasitology ; Humans ; Infant ; Infant, Newborn ; Infections ; Infectious Diseases/Epidemiology and Control of Infectious Diseases ; Livestock ; Male ; Middle Aged ; Molecular Sequence Data ; Monkeys & apes ; National parks ; Parasites ; Phylogenetics ; Polymerase Chain Reaction ; Prevalence ; Primates ; Risk Factors ; Sequence Analysis, DNA ; Sequence Homology ; Studies ; Towns ; Tropical diseases ; Uganda - epidemiology ; Wildlife conservation ; Young Adult</subject><ispartof>PLoS neglected tropical diseases, 2010-05, Vol.4 (5), p.e683-e683</ispartof><rights>2010 Johnston et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Johnston AR, Gillespie TR, Rwego IB, Tranby McLachlan TL, Kent AD, et al. (2010) Molecular Epidemiology of Cross-Species Giardia duodenalis Transmission in Western Uganda. PLoS Negl Trop Dis 4(5): e683. doi:10.1371/journal.pntd.0000683</rights><rights>Johnston et al. 2010</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c557t-24cbfa0f2c972be9f9669407a47f41282306f2270a84e4733ae82fd5a08164dc3</citedby><cites>FETCH-LOGICAL-c557t-24cbfa0f2c972be9f9669407a47f41282306f2270a84e4733ae82fd5a08164dc3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2867944/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2867944/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20485494$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Johnston, Amanda R</creatorcontrib><creatorcontrib>Gillespie, Thomas R</creatorcontrib><creatorcontrib>Rwego, Innocent B</creatorcontrib><creatorcontrib>McLachlan, Traci L Tranby</creatorcontrib><creatorcontrib>Kent, Angela D</creatorcontrib><creatorcontrib>Goldberg, Tony L</creatorcontrib><title>Molecular epidemiology of cross-species Giardia duodenalis transmission in western Uganda</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Giardia duodenalis is prevalent in tropical settings where diverse opportunities exist for transmission between people and animals. We conducted a cross-sectional study of G. duodenalis in people, livestock, and wild primates near Kibale National Park, Uganda, where human-livestock-wildlife interaction is high due to habitat disturbance. Our goal was to infer the cross-species transmission potential of G. duodenalis using molecular methods and to investigate clinical consequences of infection.
Real-time PCR on DNA extracted from fecal samples revealed a combined prevalence of G. duodenalis in people from three villages of 44/108 (40.7%), with prevalence reaching 67.5% in one village. Prevalence rates in livestock and primates were 12.4% and 11.1%, respectively. Age was associated with G. duodenalis infection in people (higher prevalence in individuals <or=15 years) and livestock (higher prevalence in subadult versus adult animals), but other potential risk factors in people (gender, contact with domestic animals, working in fields, working in forests, source of drinking water, and medication use) were not. G. duodenalis infection was not associated with gastrointestinal symptoms in people, nor was clinical disease noted in livestock or primates. Sequence analysis of four G. duodenalis genes identified assemblage AII in humans, assemblage BIV in humans and endangered red colobus monkeys, and assemblage E in livestock and red colobus, representing the first documentation of assemblage E in a non-human primate. In addition, genetic relationships within the BIV assemblage revealed sub-clades of identical G. duodenalis sequences from humans and red colobus.
Our finding of G. duodenalis in people and primates (assemblage BIV) and livestock and primates (assemblage E) underscores that cross-species transmission of multiple G. duodenalis assemblages may occur in locations such as western Uganda where people, livestock, and primates overlap in their use of habitat. Our data also demonstrate a high but locally variable prevalence of G. duodenalis in people from western Uganda, but little evidence of associated clinical disease. Reverse zoonotic G. duodenalis transmission may be particularly frequent in tropical settings where anthropogenic habitat disturbance forces people and livestock to interact at high rates with wildlife, and this could have negative consequences for wildlife conservation.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Animal diseases</subject><subject>Animals</subject><subject>Animals, Domestic - parasitology</subject><subject>Anthropogenic factors</subject><subject>Cattle</subject><subject>Child</subject><subject>Child, Preschool</subject><subject>Cluster Analysis</subject><subject>Colobus</subject><subject>Cross-Sectional Studies</subject><subject>Disease transmission</subject><subject>DNA, Protozoan - chemistry</subject><subject>DNA, Protozoan - genetics</subject><subject>Domestic animals</subject><subject>Drinking water</subject><subject>Ecology</subject><subject>Environmental protection</subject><subject>Epidemiology</subject><subject>Feces - parasitology</subject><subject>Female</subject><subject>Genotype</subject><subject>Giardia duodenalis</subject><subject>Giardia lamblia - classification</subject><subject>Giardia lamblia - genetics</subject><subject>Giardia lamblia - isolation & purification</subject><subject>Giardiasis - epidemiology</subject><subject>Giardiasis - parasitology</subject><subject>Giardiasis - transmission</subject><subject>Giardiasis - veterinary</subject><subject>Habitat utilization</subject><subject>Habitats</subject><subject>Haplorhini - parasitology</subject><subject>Humans</subject><subject>Infant</subject><subject>Infant, Newborn</subject><subject>Infections</subject><subject>Infectious Diseases/Epidemiology and Control of Infectious Diseases</subject><subject>Livestock</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Molecular Sequence Data</subject><subject>Monkeys & apes</subject><subject>National parks</subject><subject>Parasites</subject><subject>Phylogenetics</subject><subject>Polymerase Chain Reaction</subject><subject>Prevalence</subject><subject>Primates</subject><subject>Risk Factors</subject><subject>Sequence Analysis, DNA</subject><subject>Sequence Homology</subject><subject>Studies</subject><subject>Towns</subject><subject>Tropical diseases</subject><subject>Uganda - epidemiology</subject><subject>Wildlife conservation</subject><subject>Young Adult</subject><issn>1935-2735</issn><issn>1935-2727</issn><issn>1935-2735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqFUk1rFTEUHUSxtfoPRAdcuJpnPieTjSBFa6Hixi5chTv5eOaRl4zJjNJ_b9o3La0IZpOQe865uSenaV5itMFU4He7tOQIYTPF2WxQXf1AHzXHWFLeEUH543vno-ZZKTuEuOQDftocEcQGziQ7br5_ScHqJUBu7eSN3fsU0vaqTa7VOZXSlclqb0t75iEbD61ZkrG1ry_tnCGWvS_Fp9j62P62ZbY5tpdbiAaeN08chGJfrPtJc_np47fTz93F17Pz0w8XneZczB1henSAHNFSkNFKJ_teMiSACccwGQhFvSNEIBiYZYJSsANxhgMacM-MpifN64PuFFJRqytFVeqAMa7sijg_IEyCnZqy30O-Ugm8urlIeasgz14Hq4QjEkbBpeaCaQJDL0ZBtCBCC6yBVK33a7dl3FujbawuhAeiDyvR_1Db9EuRKiUZqwJvV4Gcfi7VMVUd1DYEiDYtRQnWU8QZp_9HUsrZIAWvyDd_If9tAzugbj42W3f3aozUdaRuWeo6UmqNVKW9uj_xHek2Q_QPaVzKQA</recordid><startdate>20100511</startdate><enddate>20100511</enddate><creator>Johnston, Amanda R</creator><creator>Gillespie, Thomas R</creator><creator>Rwego, Innocent B</creator><creator>McLachlan, Traci L Tranby</creator><creator>Kent, Angela D</creator><creator>Goldberg, Tony L</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7T2</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20100511</creationdate><title>Molecular epidemiology of cross-species Giardia duodenalis transmission in western Uganda</title><author>Johnston, Amanda R ; Gillespie, Thomas R ; Rwego, Innocent B ; McLachlan, Traci L Tranby ; Kent, Angela D ; Goldberg, Tony L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c557t-24cbfa0f2c972be9f9669407a47f41282306f2270a84e4733ae82fd5a08164dc3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Animal diseases</topic><topic>Animals</topic><topic>Animals, Domestic - parasitology</topic><topic>Anthropogenic factors</topic><topic>Cattle</topic><topic>Child</topic><topic>Child, Preschool</topic><topic>Cluster Analysis</topic><topic>Colobus</topic><topic>Cross-Sectional Studies</topic><topic>Disease transmission</topic><topic>DNA, Protozoan - chemistry</topic><topic>DNA, Protozoan - genetics</topic><topic>Domestic animals</topic><topic>Drinking water</topic><topic>Ecology</topic><topic>Environmental protection</topic><topic>Epidemiology</topic><topic>Feces - parasitology</topic><topic>Female</topic><topic>Genotype</topic><topic>Giardia duodenalis</topic><topic>Giardia lamblia - classification</topic><topic>Giardia lamblia - genetics</topic><topic>Giardia lamblia - isolation & purification</topic><topic>Giardiasis - epidemiology</topic><topic>Giardiasis - parasitology</topic><topic>Giardiasis - transmission</topic><topic>Giardiasis - veterinary</topic><topic>Habitat utilization</topic><topic>Habitats</topic><topic>Haplorhini - parasitology</topic><topic>Humans</topic><topic>Infant</topic><topic>Infant, Newborn</topic><topic>Infections</topic><topic>Infectious Diseases/Epidemiology and Control of Infectious Diseases</topic><topic>Livestock</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Molecular Sequence Data</topic><topic>Monkeys & apes</topic><topic>National parks</topic><topic>Parasites</topic><topic>Phylogenetics</topic><topic>Polymerase Chain Reaction</topic><topic>Prevalence</topic><topic>Primates</topic><topic>Risk Factors</topic><topic>Sequence Analysis, DNA</topic><topic>Sequence Homology</topic><topic>Studies</topic><topic>Towns</topic><topic>Tropical diseases</topic><topic>Uganda - epidemiology</topic><topic>Wildlife conservation</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Johnston, Amanda R</creatorcontrib><creatorcontrib>Gillespie, Thomas R</creatorcontrib><creatorcontrib>Rwego, Innocent B</creatorcontrib><creatorcontrib>McLachlan, Traci L Tranby</creatorcontrib><creatorcontrib>Kent, Angela D</creatorcontrib><creatorcontrib>Goldberg, Tony L</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PLoS neglected tropical diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Johnston, Amanda R</au><au>Gillespie, Thomas R</au><au>Rwego, Innocent B</au><au>McLachlan, Traci L Tranby</au><au>Kent, Angela D</au><au>Goldberg, Tony L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular epidemiology of cross-species Giardia duodenalis transmission in western Uganda</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2010-05-11</date><risdate>2010</risdate><volume>4</volume><issue>5</issue><spage>e683</spage><epage>e683</epage><pages>e683-e683</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>Giardia duodenalis is prevalent in tropical settings where diverse opportunities exist for transmission between people and animals. We conducted a cross-sectional study of G. duodenalis in people, livestock, and wild primates near Kibale National Park, Uganda, where human-livestock-wildlife interaction is high due to habitat disturbance. Our goal was to infer the cross-species transmission potential of G. duodenalis using molecular methods and to investigate clinical consequences of infection.
Real-time PCR on DNA extracted from fecal samples revealed a combined prevalence of G. duodenalis in people from three villages of 44/108 (40.7%), with prevalence reaching 67.5% in one village. Prevalence rates in livestock and primates were 12.4% and 11.1%, respectively. Age was associated with G. duodenalis infection in people (higher prevalence in individuals <or=15 years) and livestock (higher prevalence in subadult versus adult animals), but other potential risk factors in people (gender, contact with domestic animals, working in fields, working in forests, source of drinking water, and medication use) were not. G. duodenalis infection was not associated with gastrointestinal symptoms in people, nor was clinical disease noted in livestock or primates. Sequence analysis of four G. duodenalis genes identified assemblage AII in humans, assemblage BIV in humans and endangered red colobus monkeys, and assemblage E in livestock and red colobus, representing the first documentation of assemblage E in a non-human primate. In addition, genetic relationships within the BIV assemblage revealed sub-clades of identical G. duodenalis sequences from humans and red colobus.
Our finding of G. duodenalis in people and primates (assemblage BIV) and livestock and primates (assemblage E) underscores that cross-species transmission of multiple G. duodenalis assemblages may occur in locations such as western Uganda where people, livestock, and primates overlap in their use of habitat. Our data also demonstrate a high but locally variable prevalence of G. duodenalis in people from western Uganda, but little evidence of associated clinical disease. Reverse zoonotic G. duodenalis transmission may be particularly frequent in tropical settings where anthropogenic habitat disturbance forces people and livestock to interact at high rates with wildlife, and this could have negative consequences for wildlife conservation.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>20485494</pmid><doi>10.1371/journal.pntd.0000683</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1935-2735 |
| ispartof | PLoS neglected tropical diseases, 2010-05, Vol.4 (5), p.e683-e683 |
| issn | 1935-2735 1935-2727 1935-2735 |
| language | eng |
| recordid | cdi_plos_journals_1288111306 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central; PubMed Central Open Access; Public Library of Science (PLoS) |
| subjects | Adolescent Adult Aged Animal diseases Animals Animals, Domestic - parasitology Anthropogenic factors Cattle Child Child, Preschool Cluster Analysis Colobus Cross-Sectional Studies Disease transmission DNA, Protozoan - chemistry DNA, Protozoan - genetics Domestic animals Drinking water Ecology Environmental protection Epidemiology Feces - parasitology Female Genotype Giardia duodenalis Giardia lamblia - classification Giardia lamblia - genetics Giardia lamblia - isolation & purification Giardiasis - epidemiology Giardiasis - parasitology Giardiasis - transmission Giardiasis - veterinary Habitat utilization Habitats Haplorhini - parasitology Humans Infant Infant, Newborn Infections Infectious Diseases/Epidemiology and Control of Infectious Diseases Livestock Male Middle Aged Molecular Sequence Data Monkeys & apes National parks Parasites Phylogenetics Polymerase Chain Reaction Prevalence Primates Risk Factors Sequence Analysis, DNA Sequence Homology Studies Towns Tropical diseases Uganda - epidemiology Wildlife conservation Young Adult |
| title | Molecular epidemiology of cross-species Giardia duodenalis transmission in western Uganda |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-02T19%3A56%3A53IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Molecular%20epidemiology%20of%20cross-species%20Giardia%20duodenalis%20transmission%20in%20western%20Uganda&rft.jtitle=PLoS%20neglected%20tropical%20diseases&rft.au=Johnston,%20Amanda%20R&rft.date=2010-05-11&rft.volume=4&rft.issue=5&rft.spage=e683&rft.epage=e683&rft.pages=e683-e683&rft.issn=1935-2735&rft.eissn=1935-2735&rft_id=info:doi/10.1371/journal.pntd.0000683&rft_dat=%3Cproquest_plos_%3E733548975%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1288111306&rft_id=info:pmid/20485494&rft_doaj_id=oai_doaj_org_article_7f29ab759c574c2a867b72c727c71ca2&rfr_iscdi=true |