Luciferase-expressing Leishmania infantum allows the monitoring of amastigote population size, in vivo, ex vivo and in vitro
Here we engineered transgenic Leishmania infantum that express luciferase, the objectives being to more easily monitor in real time their establishment either in BALB/c mice--the liver and spleen being mainly studied-or in vitro. Whatever stationary phase L. infantum promastigotes population--wild t...
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| Veröffentlicht in: | PLoS neglected tropical diseases 2011-09, Vol.5 (9), p.e1323-e1323 |
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| creator | Michel, Grégory Ferrua, Bernard Lang, Thierry Maddugoda, Madhavi P Munro, Patrick Pomares, Christelle Lemichez, Emmanuel Marty, Pierre |
| description | Here we engineered transgenic Leishmania infantum that express luciferase, the objectives being to more easily monitor in real time their establishment either in BALB/c mice--the liver and spleen being mainly studied-or in vitro. Whatever stationary phase L. infantum promastigotes population--wild type or engineered to express luciferase-the parasite burden was similar in the liver and the spleen at day 30 post the intravenous inoculation of BALB/c mice. Imaging of L. infantum hosting BALB/C mice provided sensitivity in the range of 20,000 to 40,000 amastigotes/mg tissue, two tissues-liver and spleen-being monitored. Once sampled and processed ex vivo for their luciferin-dependent bioluminescence the threshold sensitivity was shown to range from 1,000 to 6,000 amastigotes/mg tissue. This model further proved to be valuable for in vivo measurement of the efficiency of drugs such as miltefosine and may, therefore, additionally be used to evaluate vaccine-induced protection. |
| doi_str_mv | 10.1371/journal.pntd.0001323 |
| format | Article |
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Whatever stationary phase L. infantum promastigotes population--wild type or engineered to express luciferase-the parasite burden was similar in the liver and the spleen at day 30 post the intravenous inoculation of BALB/c mice. Imaging of L. infantum hosting BALB/C mice provided sensitivity in the range of 20,000 to 40,000 amastigotes/mg tissue, two tissues-liver and spleen-being monitored. Once sampled and processed ex vivo for their luciferin-dependent bioluminescence the threshold sensitivity was shown to range from 1,000 to 6,000 amastigotes/mg tissue. This model further proved to be valuable for in vivo measurement of the efficiency of drugs such as miltefosine and may, therefore, additionally be used to evaluate vaccine-induced protection.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0001323</identifier><identifier>PMID: 21931877</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Antiprotozoal Agents ; Antiprotozoal Agents - pharmacology ; Biology ; Bioluminescence ; Care and treatment ; Drug Evaluation, Preclinical ; Drug Evaluation, Preclinical - methods ; Drugs ; Efficiency ; Female ; Genes ; Health aspects ; Human health and pathology ; Immunization ; Infectious diseases ; Laboratory animals ; Leishmania infantum ; Leishmania infantum - enzymology ; Leishmania infantum - genetics ; Leishmania infantum - isolation & purification ; Leishmaniasis ; Leishmaniasis, Visceral ; Leishmaniasis, Visceral - drug therapy ; Leishmaniasis, Visceral - parasitology ; Life Cycle Stages ; Life Sciences ; Liver ; Liver - parasitology ; Luciferase ; Luciferases ; Luciferases - analysis ; Luciferases - biosynthesis ; Luciferases - chemistry ; Luciferases - genetics ; Luciferin ; Luminescent Measurements ; Mice ; Mice, Inbred BALB C ; Organisms, Genetically Modified ; Parasite Load ; Parasite Load - methods ; Parasites ; Parasitic diseases ; Phosphorylcholine ; Phosphorylcholine - analogs & derivatives ; Phosphorylcholine - pharmacology ; Population number ; Protozoa ; Protozoan Proteins ; Reproducibility of Results ; Sensitivity and Specificity ; Spectrum Analysis ; Spectrum Analysis - methods ; Spleen ; Spleen - parasitology ; Statistics, Nonparametric ; Tropical diseases ; Vaccines ; Whole Body Imaging</subject><ispartof>PLoS neglected tropical diseases, 2011-09, Vol.5 (9), p.e1323-e1323</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Michel et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Michel G, Ferrua B, Lang T, Maddugoda MP, Munro P, et al. (2011) Luciferase-Expressing Leishmania infantum Allows the Monitoring of Amastigote Population Size, In Vivo, Ex Vivo and In Vitro. PLoS Negl Trop Dis 5(9): e1323. doi:10.1371/journal.pntd.0001323</rights><rights>Distributed under a Creative Commons Attribution 4.0 International License</rights><rights>Michel et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c660t-c0eeed0447d439ae429bb8a65b414fbf22c9c234828978a6778716db12e93613</citedby><cites>FETCH-LOGICAL-c660t-c0eeed0447d439ae429bb8a65b414fbf22c9c234828978a6778716db12e93613</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172198/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3172198/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,724,777,781,861,882,2096,2915,23847,27905,27906,53772,53774,79349,79350</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21931877$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://inserm.hal.science/inserm-00691453$$DView record in HAL$$Hfree_for_read</backlink></links><search><contributor>Milon, Genevieve</contributor><creatorcontrib>Michel, Grégory</creatorcontrib><creatorcontrib>Ferrua, Bernard</creatorcontrib><creatorcontrib>Lang, Thierry</creatorcontrib><creatorcontrib>Maddugoda, Madhavi P</creatorcontrib><creatorcontrib>Munro, Patrick</creatorcontrib><creatorcontrib>Pomares, Christelle</creatorcontrib><creatorcontrib>Lemichez, Emmanuel</creatorcontrib><creatorcontrib>Marty, Pierre</creatorcontrib><title>Luciferase-expressing Leishmania infantum allows the monitoring of amastigote population size, in vivo, ex vivo and in vitro</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Here we engineered transgenic Leishmania infantum that express luciferase, the objectives being to more easily monitor in real time their establishment either in BALB/c mice--the liver and spleen being mainly studied-or in vitro. Whatever stationary phase L. infantum promastigotes population--wild type or engineered to express luciferase-the parasite burden was similar in the liver and the spleen at day 30 post the intravenous inoculation of BALB/c mice. Imaging of L. infantum hosting BALB/C mice provided sensitivity in the range of 20,000 to 40,000 amastigotes/mg tissue, two tissues-liver and spleen-being monitored. Once sampled and processed ex vivo for their luciferin-dependent bioluminescence the threshold sensitivity was shown to range from 1,000 to 6,000 amastigotes/mg tissue. This model further proved to be valuable for in vivo measurement of the efficiency of drugs such as miltefosine and may, therefore, additionally be used to evaluate vaccine-induced protection.</description><subject>Animals</subject><subject>Antiprotozoal Agents</subject><subject>Antiprotozoal Agents - pharmacology</subject><subject>Biology</subject><subject>Bioluminescence</subject><subject>Care and treatment</subject><subject>Drug Evaluation, Preclinical</subject><subject>Drug Evaluation, Preclinical - methods</subject><subject>Drugs</subject><subject>Efficiency</subject><subject>Female</subject><subject>Genes</subject><subject>Health aspects</subject><subject>Human health and pathology</subject><subject>Immunization</subject><subject>Infectious diseases</subject><subject>Laboratory animals</subject><subject>Leishmania infantum</subject><subject>Leishmania infantum - enzymology</subject><subject>Leishmania infantum - genetics</subject><subject>Leishmania infantum - isolation & purification</subject><subject>Leishmaniasis</subject><subject>Leishmaniasis, Visceral</subject><subject>Leishmaniasis, Visceral - drug therapy</subject><subject>Leishmaniasis, Visceral - parasitology</subject><subject>Life Cycle Stages</subject><subject>Life Sciences</subject><subject>Liver</subject><subject>Liver - parasitology</subject><subject>Luciferase</subject><subject>Luciferases</subject><subject>Luciferases - analysis</subject><subject>Luciferases - biosynthesis</subject><subject>Luciferases - chemistry</subject><subject>Luciferases - genetics</subject><subject>Luciferin</subject><subject>Luminescent Measurements</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Organisms, Genetically Modified</subject><subject>Parasite Load</subject><subject>Parasite Load - methods</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Phosphorylcholine</subject><subject>Phosphorylcholine - analogs & derivatives</subject><subject>Phosphorylcholine - pharmacology</subject><subject>Population number</subject><subject>Protozoa</subject><subject>Protozoan Proteins</subject><subject>Reproducibility of Results</subject><subject>Sensitivity and Specificity</subject><subject>Spectrum Analysis</subject><subject>Spectrum Analysis - methods</subject><subject>Spleen</subject><subject>Spleen - parasitology</subject><subject>Statistics, Nonparametric</subject><subject>Tropical diseases</subject><subject>Vaccines</subject><subject>Whole Body Imaging</subject><issn>1935-2735</issn><issn>1935-2727</issn><issn>1935-2735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNptUk1v1DAQjRCIlsI_QBAJCS67iz_ixL4grSqglVbi0rvlJJONV4kdbGdbED8ep5tWu1Xlg0fj9954Zl6SvMdohWmBv-7s6IzqVoMJ9QohhCmhL5JzLChbkoKyl0fxWfLG-x1CTDCOXydnJL5gXhTnyb_NWOkGnPKwhLvBgffabNMNaN_2ymiVatMoE8Y-VV1nb30aWkh7a3SwbkLaJlW98kFvbYB0sMPYqaCtSb3-C4vITvd6bxcp3N0HqTL1IRmcfZu8alTn4d18XyQ3P77fXF4tN79-Xl-uN8sqz1FYVggAapRlRZ1RoSAjoiy5ylmZ4awpG0IqURGaccJFEfNFwQuc1yUmIGiO6UXy8SA7dNbLeW5eYsI5xpgwGhHXB0Rt1U4OTvfK_ZFWaXmfsG4rlQu66kASgKrBjOQ5LzNFas64ahChdComVBa1vs3VxrKHugITnOpORE9fjG7l1u4lxUXcC48Ci4NA-4R2td5IbTy4XiKUC5wxup-6-zLXc_b3CD7IXvsKuk4ZsKOXXFAiEGcsIj89QT4_ixm1VbHduH0bf1lNmnJNcsEopvnU5eoZVDw19LqyBhod8yeEz0eEFlQXWm-7cfKKPwVmB2DlrPcOmscZYCQn5z_8Wk7Ol7PzI-3D8dgfSQ9Wp_8BZ0EAUg</recordid><startdate>20110901</startdate><enddate>20110901</enddate><creator>Michel, Grégory</creator><creator>Ferrua, Bernard</creator><creator>Lang, Thierry</creator><creator>Maddugoda, Madhavi P</creator><creator>Munro, Patrick</creator><creator>Pomares, Christelle</creator><creator>Lemichez, Emmanuel</creator><creator>Marty, Pierre</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7T2</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>1XC</scope><scope>VOOES</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20110901</creationdate><title>Luciferase-expressing Leishmania infantum allows the monitoring of amastigote population size, in vivo, ex vivo and in vitro</title><author>Michel, Grégory ; Ferrua, Bernard ; Lang, Thierry ; Maddugoda, Madhavi P ; Munro, Patrick ; Pomares, Christelle ; Lemichez, Emmanuel ; Marty, Pierre</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c660t-c0eeed0447d439ae429bb8a65b414fbf22c9c234828978a6778716db12e93613</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Animals</topic><topic>Antiprotozoal Agents</topic><topic>Antiprotozoal Agents - pharmacology</topic><topic>Biology</topic><topic>Bioluminescence</topic><topic>Care and treatment</topic><topic>Drug Evaluation, Preclinical</topic><topic>Drug Evaluation, Preclinical - methods</topic><topic>Drugs</topic><topic>Efficiency</topic><topic>Female</topic><topic>Genes</topic><topic>Health aspects</topic><topic>Human health and pathology</topic><topic>Immunization</topic><topic>Infectious diseases</topic><topic>Laboratory animals</topic><topic>Leishmania infantum</topic><topic>Leishmania infantum - enzymology</topic><topic>Leishmania infantum - genetics</topic><topic>Leishmania infantum - isolation & purification</topic><topic>Leishmaniasis</topic><topic>Leishmaniasis, Visceral</topic><topic>Leishmaniasis, Visceral - drug therapy</topic><topic>Leishmaniasis, Visceral - parasitology</topic><topic>Life Cycle Stages</topic><topic>Life Sciences</topic><topic>Liver</topic><topic>Liver - parasitology</topic><topic>Luciferase</topic><topic>Luciferases</topic><topic>Luciferases - analysis</topic><topic>Luciferases - 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| fulltext | fulltext |
| identifier | ISSN: 1935-2735 |
| ispartof | PLoS neglected tropical diseases, 2011-09, Vol.5 (9), p.e1323-e1323 |
| issn | 1935-2735 1935-2727 1935-2735 |
| language | eng |
| recordid | cdi_plos_journals_1288111253 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Antiprotozoal Agents Antiprotozoal Agents - pharmacology Biology Bioluminescence Care and treatment Drug Evaluation, Preclinical Drug Evaluation, Preclinical - methods Drugs Efficiency Female Genes Health aspects Human health and pathology Immunization Infectious diseases Laboratory animals Leishmania infantum Leishmania infantum - enzymology Leishmania infantum - genetics Leishmania infantum - isolation & purification Leishmaniasis Leishmaniasis, Visceral Leishmaniasis, Visceral - drug therapy Leishmaniasis, Visceral - parasitology Life Cycle Stages Life Sciences Liver Liver - parasitology Luciferase Luciferases Luciferases - analysis Luciferases - biosynthesis Luciferases - chemistry Luciferases - genetics Luciferin Luminescent Measurements Mice Mice, Inbred BALB C Organisms, Genetically Modified Parasite Load Parasite Load - methods Parasites Parasitic diseases Phosphorylcholine Phosphorylcholine - analogs & derivatives Phosphorylcholine - pharmacology Population number Protozoa Protozoan Proteins Reproducibility of Results Sensitivity and Specificity Spectrum Analysis Spectrum Analysis - methods Spleen Spleen - parasitology Statistics, Nonparametric Tropical diseases Vaccines Whole Body Imaging |
| title | Luciferase-expressing Leishmania infantum allows the monitoring of amastigote population size, in vivo, ex vivo and in vitro |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-18T00%3A14%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Luciferase-expressing%20Leishmania%20infantum%20allows%20the%20monitoring%20of%20amastigote%20population%20size,%20in%20vivo,%20ex%20vivo%20and%20in%20vitro&rft.jtitle=PLoS%20neglected%20tropical%20diseases&rft.au=Michel,%20Gr%C3%A9gory&rft.date=2011-09-01&rft.volume=5&rft.issue=9&rft.spage=e1323&rft.epage=e1323&rft.pages=e1323-e1323&rft.issn=1935-2735&rft.eissn=1935-2735&rft_id=info:doi/10.1371/journal.pntd.0001323&rft_dat=%3Cgale_plos_%3EA269531364%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1288111253&rft_id=info:pmid/21931877&rft_galeid=A269531364&rft_doaj_id=oai_doaj_org_article_2eecf152668b4a2d858af02332e939a4&rfr_iscdi=true |