Evaluation of the human IgG antibody response to Aedes albopictus saliva as a new specific biomarker of exposure to vector bites
The spread of Aedes albopictus, a vector for re-emergent arbovirus diseases like chikungunya and dengue, points up the need for better control strategies and new tools to evaluate transmission risk. Human antibody (Ab) responses to mosquito salivary proteins could represent a reliable biomarker for...
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| Veröffentlicht in: | PLoS neglected tropical diseases 2012-02, Vol.6 (2), p.e1487 |
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| creator | Doucoure, Souleymane Mouchet, François Cornelie, Sylvie DeHecq, Jean Sébastien Rutee, Abdul Hamid Roca, Yelin Walter, Annie Hervé, Jean Pierre Misse, Dorothée Favier, François Gasque, Philippe Remoue, Franck |
| description | The spread of Aedes albopictus, a vector for re-emergent arbovirus diseases like chikungunya and dengue, points up the need for better control strategies and new tools to evaluate transmission risk. Human antibody (Ab) responses to mosquito salivary proteins could represent a reliable biomarker for evaluating human-vector contact and the efficacy of control programs.
We used ELISA tests to evaluate specific immunoglobulin G (IgG) responses to salivary gland extracts (SGE) in adults exposed to Aedes albopictus in Reunion Island. The percentage of immune responders (88%) and levels of anti-SGE IgG Abs were high in exposed individuals. At an individual level, our results indicate heterogeneity of the exposure to Aedes albopictus bites. In addition, low-level immune cross-reactivity between Aedes albopictus and Aedes aegypti SGEs was observed, mainly in the highest responders.
Ab responses to saliva could be used as an immuno-epidemiological tool for evaluating exposure to Aedes albopictus bites. Combined with entomological and epidemiological methods, a "salivary" biomarker of exposure to Aedes albopictus could enhance surveillance of its spread and the risk of arbovirus transmission, and could be used as a direct tool for the evaluation of Aedes albopictus control strategies. |
| doi_str_mv | 10.1371/journal.pntd.0001487 |
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We used ELISA tests to evaluate specific immunoglobulin G (IgG) responses to salivary gland extracts (SGE) in adults exposed to Aedes albopictus in Reunion Island. The percentage of immune responders (88%) and levels of anti-SGE IgG Abs were high in exposed individuals. At an individual level, our results indicate heterogeneity of the exposure to Aedes albopictus bites. In addition, low-level immune cross-reactivity between Aedes albopictus and Aedes aegypti SGEs was observed, mainly in the highest responders.
Ab responses to saliva could be used as an immuno-epidemiological tool for evaluating exposure to Aedes albopictus bites. Combined with entomological and epidemiological methods, a "salivary" biomarker of exposure to Aedes albopictus could enhance surveillance of its spread and the risk of arbovirus transmission, and could be used as a direct tool for the evaluation of Aedes albopictus control strategies.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0001487</identifier><identifier>PMID: 22363823</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Adolescent ; Adult ; Aedes - immunology ; Aedes albopictus ; Animals ; Antibody Formation ; Biological markers ; Biology ; Biomarkers - blood ; Clinical Laboratory Techniques - methods ; Emerging diseases ; Enzyme-Linked Immunosorbent Assay - methods ; Female ; Genetic vectors ; Health aspects ; Human health and pathology ; Humans ; Identification and classification ; Immune response ; Immunoglobulin G ; Immunoglobulin G - blood ; Immunology ; Infectious diseases ; Insect Bites and Stings - diagnosis ; Life Sciences ; Medicine ; Properties ; Reunion ; Saliva - immunology ; Salivary Proteins and Peptides - immunology ; Santé publique et épidémiologie ; Young Adult</subject><ispartof>PLoS neglected tropical diseases, 2012-02, Vol.6 (2), p.e1487</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Attribution</rights><rights>Doucoure et al. 2012</rights><rights>2012 Doucoure et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Doucoure S, Mouchet F, Cornelie S, DeHecq JS, Rutee AH, et al. (2012) Evaluation of the Human IgG Antibody Response to Aedes albopictus Saliva as a New Specific Biomarker of Exposure to Vector Bites. PLoS Negl Trop Dis 6(2): e1487. doi:10.1371/journal.pntd.0001487</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c629t-642dfb7d04e4ec6ff2c4cbd9f8035f8ca699299aab9e66ee80944b2fed20296b3</citedby><cites>FETCH-LOGICAL-c629t-642dfb7d04e4ec6ff2c4cbd9f8035f8ca699299aab9e66ee80944b2fed20296b3</cites><orcidid>0000-0002-6485-3841 ; 0000-0003-3662-1450 ; 0000-0003-1837-8063 ; 0000-0003-3220-5660</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283547/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3283547/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22363823$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://hal.univ-reunion.fr/hal-01274594$$DView record in HAL$$Hfree_for_read</backlink></links><search><creatorcontrib>Doucoure, Souleymane</creatorcontrib><creatorcontrib>Mouchet, François</creatorcontrib><creatorcontrib>Cornelie, Sylvie</creatorcontrib><creatorcontrib>DeHecq, Jean Sébastien</creatorcontrib><creatorcontrib>Rutee, Abdul Hamid</creatorcontrib><creatorcontrib>Roca, Yelin</creatorcontrib><creatorcontrib>Walter, Annie</creatorcontrib><creatorcontrib>Hervé, Jean Pierre</creatorcontrib><creatorcontrib>Misse, Dorothée</creatorcontrib><creatorcontrib>Favier, François</creatorcontrib><creatorcontrib>Gasque, Philippe</creatorcontrib><creatorcontrib>Remoue, Franck</creatorcontrib><title>Evaluation of the human IgG antibody response to Aedes albopictus saliva as a new specific biomarker of exposure to vector bites</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>The spread of Aedes albopictus, a vector for re-emergent arbovirus diseases like chikungunya and dengue, points up the need for better control strategies and new tools to evaluate transmission risk. Human antibody (Ab) responses to mosquito salivary proteins could represent a reliable biomarker for evaluating human-vector contact and the efficacy of control programs.
We used ELISA tests to evaluate specific immunoglobulin G (IgG) responses to salivary gland extracts (SGE) in adults exposed to Aedes albopictus in Reunion Island. The percentage of immune responders (88%) and levels of anti-SGE IgG Abs were high in exposed individuals. At an individual level, our results indicate heterogeneity of the exposure to Aedes albopictus bites. In addition, low-level immune cross-reactivity between Aedes albopictus and Aedes aegypti SGEs was observed, mainly in the highest responders.
Ab responses to saliva could be used as an immuno-epidemiological tool for evaluating exposure to Aedes albopictus bites. 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Human antibody (Ab) responses to mosquito salivary proteins could represent a reliable biomarker for evaluating human-vector contact and the efficacy of control programs.
We used ELISA tests to evaluate specific immunoglobulin G (IgG) responses to salivary gland extracts (SGE) in adults exposed to Aedes albopictus in Reunion Island. The percentage of immune responders (88%) and levels of anti-SGE IgG Abs were high in exposed individuals. At an individual level, our results indicate heterogeneity of the exposure to Aedes albopictus bites. In addition, low-level immune cross-reactivity between Aedes albopictus and Aedes aegypti SGEs was observed, mainly in the highest responders.
Ab responses to saliva could be used as an immuno-epidemiological tool for evaluating exposure to Aedes albopictus bites. Combined with entomological and epidemiological methods, a "salivary" biomarker of exposure to Aedes albopictus could enhance surveillance of its spread and the risk of arbovirus transmission, and could be used as a direct tool for the evaluation of Aedes albopictus control strategies.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22363823</pmid><doi>10.1371/journal.pntd.0001487</doi><orcidid>https://orcid.org/0000-0002-6485-3841</orcidid><orcidid>https://orcid.org/0000-0003-3662-1450</orcidid><orcidid>https://orcid.org/0000-0003-1837-8063</orcidid><orcidid>https://orcid.org/0000-0003-3220-5660</orcidid><oa>free_for_read</oa></addata></record> |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Adolescent Adult Aedes - immunology Aedes albopictus Animals Antibody Formation Biological markers Biology Biomarkers - blood Clinical Laboratory Techniques - methods Emerging diseases Enzyme-Linked Immunosorbent Assay - methods Female Genetic vectors Health aspects Human health and pathology Humans Identification and classification Immune response Immunoglobulin G Immunoglobulin G - blood Immunology Infectious diseases Insect Bites and Stings - diagnosis Life Sciences Medicine Properties Reunion Saliva - immunology Salivary Proteins and Peptides - immunology Santé publique et épidémiologie Young Adult |
| title | Evaluation of the human IgG antibody response to Aedes albopictus saliva as a new specific biomarker of exposure to vector bites |
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