Utilization of ELISA using thioredoxin peroxidase-1 and tandem repeat proteins for diagnosis of Schistosoma japonicum infection among water buffaloes
The presence of animal reservoirs in Schistosoma japonicum infection has been a major obstacle in the control of schistosomiasis. Previous studies have proven that the inclusion of control measures on animal reservoir hosts for schistosomiasis contributed to the decrease of human cases. Animal surve...
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| Veröffentlicht in: | PLoS neglected tropical diseases 2012-08, Vol.6 (8), p.e1800-e1800 |
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| creator | Angeles, Jose Ma M Goto, Yasuyuki Kirinoki, Masashi Asada, Masahito Leonardo, Lydia R Rivera, Pilarita T Villacorte, Elena A Inoue, Noboru Chigusa, Yuichi Kawazu, Shin-ichiro |
| description | The presence of animal reservoirs in Schistosoma japonicum infection has been a major obstacle in the control of schistosomiasis. Previous studies have proven that the inclusion of control measures on animal reservoir hosts for schistosomiasis contributed to the decrease of human cases. Animal surveillance should therefore be included to strengthen and improve the capabilities of current serological tests.
Thioredoxin peroxidase-1 (SjTPx-1) and four tandem repeat proteins (Sj1TR, Sj2TR, Sj4TR, Sj7TR) were initially evaluated against human sera. The previous test showed high sensitivity and specificity for antibody detection against SjTPx-1 and Sj7TR. In this study, the immunodiagnostic potential of these recombinant proteins was evaluated using enzyme-linked immunoassay on 50 water buffalo serum samples collected in Cagayan, the Philippines as compared with the soluble egg antigen (SEA). For specificity, 3 goat serum samples positive with Fasciola hepatica were used and among the antigens used, only SEA showed cross-reaction. Stool PCR targeting the S. japonicum 82 bp mitochondrial NAD 1 gene was done to confirm the true positives and served as the standard test. Twenty three samples were positive for stool PCR. SjTPx-1 and Sj1TR gave the highest sensitivity among the recombinant proteins tested for water buffalo samples with 82.61% and 78.26% respectively which were higher than that of SEA (69.57%).
These results prove that SjTPx-1 works both for humans and water buffaloes making it a good candidate antigen for zoonotic diagnosis. Sj1TR showed good results for water buffaloes and therefore can also be used as a possible candidate for detecting animal schistosome infection. |
| doi_str_mv | 10.1371/journal.pntd.0001800 |
| format | Article |
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Thioredoxin peroxidase-1 (SjTPx-1) and four tandem repeat proteins (Sj1TR, Sj2TR, Sj4TR, Sj7TR) were initially evaluated against human sera. The previous test showed high sensitivity and specificity for antibody detection against SjTPx-1 and Sj7TR. In this study, the immunodiagnostic potential of these recombinant proteins was evaluated using enzyme-linked immunoassay on 50 water buffalo serum samples collected in Cagayan, the Philippines as compared with the soluble egg antigen (SEA). For specificity, 3 goat serum samples positive with Fasciola hepatica were used and among the antigens used, only SEA showed cross-reaction. Stool PCR targeting the S. japonicum 82 bp mitochondrial NAD 1 gene was done to confirm the true positives and served as the standard test. Twenty three samples were positive for stool PCR. SjTPx-1 and Sj1TR gave the highest sensitivity among the recombinant proteins tested for water buffalo samples with 82.61% and 78.26% respectively which were higher than that of SEA (69.57%).
These results prove that SjTPx-1 works both for humans and water buffaloes making it a good candidate antigen for zoonotic diagnosis. Sj1TR showed good results for water buffaloes and therefore can also be used as a possible candidate for detecting animal schistosome infection.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0001800</identifier><identifier>PMID: 22953018</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Antibodies, Helminth - blood ; Antigens ; Antigens, Helminth ; Buffaloes ; Clinical Laboratory Techniques - methods ; Cross Reactions ; Diagnosis ; Diagnostic tests ; Disease ; Enzyme-linked immunosorbent assay ; Enzyme-Linked Immunosorbent Assay - methods ; Enzymes ; Health aspects ; Humans ; Infections ; Medicine ; Parasitology - methods ; Peroxiredoxins ; Philippines ; Polymerase chain reaction ; Proteins ; Public health ; Recombinant Proteins ; Schistosomiasis ; Schistosomiasis japonica - diagnosis ; Schistosomiasis japonica - veterinary ; Sensitivity and Specificity ; Serology ; Thioredoxin ; Tropical diseases ; Veterinary Medicine - methods ; Veterinary Science ; Water buffalo ; Zoonoses</subject><ispartof>PLoS neglected tropical diseases, 2012-08, Vol.6 (8), p.e1800-e1800</ispartof><rights>COPYRIGHT 2012 Public Library of Science</rights><rights>Angeles et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Angeles JMM, Goto Y, Kirinoki M, Asada M, Leonardo LR, et al. (2012) Utilization of ELISA Using Thioredoxin Peroxidase-1 and Tandem Repeat Proteins for Diagnosis of Schistosoma japonicum Infection among Water Buffaloes. PLoS Negl Trop Dis 6(8): e1800. doi:10.1371/journal.pntd.0001800</rights><rights>2012 Angeles et al 2012 Angeles et al</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c690t-d5c437758473d9ca83d158ee6d51bf759fb255d514a2fe774c1928fa90a7b1623</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3429387/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3429387/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79343,79344</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22953018$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Mutapi, Francisca</contributor><creatorcontrib>Angeles, Jose Ma M</creatorcontrib><creatorcontrib>Goto, Yasuyuki</creatorcontrib><creatorcontrib>Kirinoki, Masashi</creatorcontrib><creatorcontrib>Asada, Masahito</creatorcontrib><creatorcontrib>Leonardo, Lydia R</creatorcontrib><creatorcontrib>Rivera, Pilarita T</creatorcontrib><creatorcontrib>Villacorte, Elena A</creatorcontrib><creatorcontrib>Inoue, Noboru</creatorcontrib><creatorcontrib>Chigusa, Yuichi</creatorcontrib><creatorcontrib>Kawazu, Shin-ichiro</creatorcontrib><title>Utilization of ELISA using thioredoxin peroxidase-1 and tandem repeat proteins for diagnosis of Schistosoma japonicum infection among water buffaloes</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>The presence of animal reservoirs in Schistosoma japonicum infection has been a major obstacle in the control of schistosomiasis. Previous studies have proven that the inclusion of control measures on animal reservoir hosts for schistosomiasis contributed to the decrease of human cases. Animal surveillance should therefore be included to strengthen and improve the capabilities of current serological tests.
Thioredoxin peroxidase-1 (SjTPx-1) and four tandem repeat proteins (Sj1TR, Sj2TR, Sj4TR, Sj7TR) were initially evaluated against human sera. The previous test showed high sensitivity and specificity for antibody detection against SjTPx-1 and Sj7TR. In this study, the immunodiagnostic potential of these recombinant proteins was evaluated using enzyme-linked immunoassay on 50 water buffalo serum samples collected in Cagayan, the Philippines as compared with the soluble egg antigen (SEA). For specificity, 3 goat serum samples positive with Fasciola hepatica were used and among the antigens used, only SEA showed cross-reaction. Stool PCR targeting the S. japonicum 82 bp mitochondrial NAD 1 gene was done to confirm the true positives and served as the standard test. Twenty three samples were positive for stool PCR. SjTPx-1 and Sj1TR gave the highest sensitivity among the recombinant proteins tested for water buffalo samples with 82.61% and 78.26% respectively which were higher than that of SEA (69.57%).
These results prove that SjTPx-1 works both for humans and water buffaloes making it a good candidate antigen for zoonotic diagnosis. Sj1TR showed good results for water buffaloes and therefore can also be used as a possible candidate for detecting animal schistosome infection.</description><subject>Animals</subject><subject>Antibodies, Helminth - blood</subject><subject>Antigens</subject><subject>Antigens, Helminth</subject><subject>Buffaloes</subject><subject>Clinical Laboratory Techniques - methods</subject><subject>Cross Reactions</subject><subject>Diagnosis</subject><subject>Diagnostic tests</subject><subject>Disease</subject><subject>Enzyme-linked immunosorbent assay</subject><subject>Enzyme-Linked Immunosorbent Assay - methods</subject><subject>Enzymes</subject><subject>Health aspects</subject><subject>Humans</subject><subject>Infections</subject><subject>Medicine</subject><subject>Parasitology - methods</subject><subject>Peroxiredoxins</subject><subject>Philippines</subject><subject>Polymerase chain reaction</subject><subject>Proteins</subject><subject>Public health</subject><subject>Recombinant Proteins</subject><subject>Schistosomiasis</subject><subject>Schistosomiasis japonica - diagnosis</subject><subject>Schistosomiasis japonica - veterinary</subject><subject>Sensitivity and Specificity</subject><subject>Serology</subject><subject>Thioredoxin</subject><subject>Tropical diseases</subject><subject>Veterinary Medicine - methods</subject><subject>Veterinary Science</subject><subject>Water buffalo</subject><subject>Zoonoses</subject><issn>1935-2735</issn><issn>1935-2727</issn><issn>1935-2735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNptUltrFDEYHUSxtfoPRAOC-LJrMplsMi9CKVULBR9qn8M3mS-7WWaSMcl4-x_-X7PttnRFArmec75LTlW9ZHTJuGTvt2GOHobl5HO_pJQyRemj6pi1XCxqycXjB_uj6llKW0pFKxR7Wh3VdSt4YRxXf66zG9xvyC54Eiw5v7y4OiVzcn5N8saFiH346TyZMJa1h4QLRsD3JJcJRxJxQshkiiGj84nYEEnvYO1DcmkneGU2LuWQwghkC1Pwzswjcd6iuYkJYyihfkDGSLrZWhgCpufVk7JJ-GK_nlTXH8-_nn1eXH75dHF2erkwq5bmRS9Mw6UUqpG8bw0o3jOhEFe9YJ2VorVdLUQ5NFBblLIxrK2VhZaC7Niq5ifV61vdaQhJ7zuaNKuVYlSumlVBXNwi-gBbPUU3QvylAzh9cxHiWkPMzgyogXOJTHGrOmw4taBYZ0Bg2_QMTWOK1od9tLkbsTfoc4ThQPTwxbuNXofvmjd1y5UsAu_2AjF8mzFlPbpkcBjAY5hL3pSrFW2l2uX95h_o_6vbo9ZQCiifEkpcsxPVp5yWIqSkbUEt_4MqoxjAmeDRunJ_QHj7gLBBGPImhWHefXg6BDa3QBNDShHtfTMY1TuX32Wtdy7Xe5cX2quHjbwn3dma_wWv5fvx</recordid><startdate>20120801</startdate><enddate>20120801</enddate><creator>Angeles, Jose Ma M</creator><creator>Goto, Yasuyuki</creator><creator>Kirinoki, Masashi</creator><creator>Asada, Masahito</creator><creator>Leonardo, Lydia R</creator><creator>Rivera, Pilarita T</creator><creator>Villacorte, Elena A</creator><creator>Inoue, Noboru</creator><creator>Chigusa, Yuichi</creator><creator>Kawazu, Shin-ichiro</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7T2</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20120801</creationdate><title>Utilization of ELISA using thioredoxin peroxidase-1 and tandem repeat proteins for diagnosis of Schistosoma japonicum infection among water buffaloes</title><author>Angeles, Jose Ma M ; Goto, Yasuyuki ; Kirinoki, Masashi ; Asada, Masahito ; Leonardo, Lydia R ; Rivera, Pilarita T ; Villacorte, Elena A ; Inoue, Noboru ; Chigusa, Yuichi ; Kawazu, Shin-ichiro</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c690t-d5c437758473d9ca83d158ee6d51bf759fb255d514a2fe774c1928fa90a7b1623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Animals</topic><topic>Antibodies, Helminth - blood</topic><topic>Antigens</topic><topic>Antigens, Helminth</topic><topic>Buffaloes</topic><topic>Clinical Laboratory Techniques - methods</topic><topic>Cross Reactions</topic><topic>Diagnosis</topic><topic>Diagnostic tests</topic><topic>Disease</topic><topic>Enzyme-linked immunosorbent assay</topic><topic>Enzyme-Linked Immunosorbent Assay - methods</topic><topic>Enzymes</topic><topic>Health aspects</topic><topic>Humans</topic><topic>Infections</topic><topic>Medicine</topic><topic>Parasitology - methods</topic><topic>Peroxiredoxins</topic><topic>Philippines</topic><topic>Polymerase chain reaction</topic><topic>Proteins</topic><topic>Public health</topic><topic>Recombinant Proteins</topic><topic>Schistosomiasis</topic><topic>Schistosomiasis japonica - diagnosis</topic><topic>Schistosomiasis japonica - veterinary</topic><topic>Sensitivity and Specificity</topic><topic>Serology</topic><topic>Thioredoxin</topic><topic>Tropical diseases</topic><topic>Veterinary Medicine - methods</topic><topic>Veterinary Science</topic><topic>Water buffalo</topic><topic>Zoonoses</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Angeles, Jose Ma M</creatorcontrib><creatorcontrib>Goto, Yasuyuki</creatorcontrib><creatorcontrib>Kirinoki, Masashi</creatorcontrib><creatorcontrib>Asada, Masahito</creatorcontrib><creatorcontrib>Leonardo, Lydia R</creatorcontrib><creatorcontrib>Rivera, Pilarita T</creatorcontrib><creatorcontrib>Villacorte, Elena A</creatorcontrib><creatorcontrib>Inoue, Noboru</creatorcontrib><creatorcontrib>Chigusa, Yuichi</creatorcontrib><creatorcontrib>Kawazu, Shin-ichiro</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><collection>DOAJ Directory of Open Access Journals</collection><jtitle>PLoS neglected tropical diseases</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Angeles, Jose Ma M</au><au>Goto, Yasuyuki</au><au>Kirinoki, Masashi</au><au>Asada, Masahito</au><au>Leonardo, Lydia R</au><au>Rivera, Pilarita T</au><au>Villacorte, Elena A</au><au>Inoue, Noboru</au><au>Chigusa, Yuichi</au><au>Kawazu, Shin-ichiro</au><au>Mutapi, Francisca</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Utilization of ELISA using thioredoxin peroxidase-1 and tandem repeat proteins for diagnosis of Schistosoma japonicum infection among water buffaloes</atitle><jtitle>PLoS neglected tropical diseases</jtitle><addtitle>PLoS Negl Trop Dis</addtitle><date>2012-08-01</date><risdate>2012</risdate><volume>6</volume><issue>8</issue><spage>e1800</spage><epage>e1800</epage><pages>e1800-e1800</pages><issn>1935-2735</issn><issn>1935-2727</issn><eissn>1935-2735</eissn><abstract>The presence of animal reservoirs in Schistosoma japonicum infection has been a major obstacle in the control of schistosomiasis. Previous studies have proven that the inclusion of control measures on animal reservoir hosts for schistosomiasis contributed to the decrease of human cases. Animal surveillance should therefore be included to strengthen and improve the capabilities of current serological tests.
Thioredoxin peroxidase-1 (SjTPx-1) and four tandem repeat proteins (Sj1TR, Sj2TR, Sj4TR, Sj7TR) were initially evaluated against human sera. The previous test showed high sensitivity and specificity for antibody detection against SjTPx-1 and Sj7TR. In this study, the immunodiagnostic potential of these recombinant proteins was evaluated using enzyme-linked immunoassay on 50 water buffalo serum samples collected in Cagayan, the Philippines as compared with the soluble egg antigen (SEA). For specificity, 3 goat serum samples positive with Fasciola hepatica were used and among the antigens used, only SEA showed cross-reaction. Stool PCR targeting the S. japonicum 82 bp mitochondrial NAD 1 gene was done to confirm the true positives and served as the standard test. Twenty three samples were positive for stool PCR. SjTPx-1 and Sj1TR gave the highest sensitivity among the recombinant proteins tested for water buffalo samples with 82.61% and 78.26% respectively which were higher than that of SEA (69.57%).
These results prove that SjTPx-1 works both for humans and water buffaloes making it a good candidate antigen for zoonotic diagnosis. Sj1TR showed good results for water buffaloes and therefore can also be used as a possible candidate for detecting animal schistosome infection.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>22953018</pmid><doi>10.1371/journal.pntd.0001800</doi><oa>free_for_read</oa></addata></record> |
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| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Animals Antibodies, Helminth - blood Antigens Antigens, Helminth Buffaloes Clinical Laboratory Techniques - methods Cross Reactions Diagnosis Diagnostic tests Disease Enzyme-linked immunosorbent assay Enzyme-Linked Immunosorbent Assay - methods Enzymes Health aspects Humans Infections Medicine Parasitology - methods Peroxiredoxins Philippines Polymerase chain reaction Proteins Public health Recombinant Proteins Schistosomiasis Schistosomiasis japonica - diagnosis Schistosomiasis japonica - veterinary Sensitivity and Specificity Serology Thioredoxin Tropical diseases Veterinary Medicine - methods Veterinary Science Water buffalo Zoonoses |
| title | Utilization of ELISA using thioredoxin peroxidase-1 and tandem repeat proteins for diagnosis of Schistosoma japonicum infection among water buffaloes |
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