Comparative microsatellite typing of new world leishmania infantum reveals low heterogeneity among populations and its recent old world origin
Leishmania infantum (syn. L. chagasi) is the causative agent of visceral leishmaniasis (VL) in the New World (NW) with endemic regions extending from southern USA to northern Argentina. The two hypotheses about the origin of VL in the NW suggest (1) recent importation of L. infantum from the Old Wor...
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| creator | Kuhls, Katrin Alam, Mohammad Zahangir Cupolillo, Elisa Ferreira, Gabriel Eduardo M Mauricio, Isabel L Oddone, Rolando Feliciangeli, M Dora Wirth, Thierry Miles, Michael A Schönian, Gabriele |
| description | Leishmania infantum (syn. L. chagasi) is the causative agent of visceral leishmaniasis (VL) in the New World (NW) with endemic regions extending from southern USA to northern Argentina. The two hypotheses about the origin of VL in the NW suggest (1) recent importation of L. infantum from the Old World (OW), or (2) an indigenous origin and a distinct taxonomic rank for the NW parasite. Multilocus microsatellite typing was applied in a survey of 98 L. infantum isolates from different NW foci. The microsatellite profiles obtained were compared to those of 308 L. infantum and 20 L. donovani strains from OW countries previously assigned to well-defined populations. Two main populations were identified for both NW and OW L. infantum. Most of the NW strains belonged to population 1, which corresponded to the OW MON-1 population. However, the NW population was much more homogeneous. A second, more heterogeneous, population comprised most Caribbean strains and corresponded to the OW non-MON-1 population. All Brazilian L. infantum strains belonged to population 1, although they represented 61% of the sample and originated from 9 states. Population analysis including the OW L. infantum populations indicated that the NW strains were more similar to MON-1 and non-MON-1 sub-populations of L. infantum from southwest Europe, than to any other OW sub-population. Moreover, similarity between NW and Southwest European L. infantum was higher than between OW L. infantum from distinct parts of the Mediterranean region, Middle East and Central Asia. No correlation was found between NW L. infantum genotypes and clinical picture or host background. This study represents the first continent-wide analysis of NW L. infantum population structure. It confirmed that the agent of VL in the NW is L. infantum and that the parasite has been recently imported multiple times to the NW from southwest Europe. |
| doi_str_mv | 10.1371/journal.pntd.0001155 |
| format | Article |
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L. chagasi) is the causative agent of visceral leishmaniasis (VL) in the New World (NW) with endemic regions extending from southern USA to northern Argentina. The two hypotheses about the origin of VL in the NW suggest (1) recent importation of L. infantum from the Old World (OW), or (2) an indigenous origin and a distinct taxonomic rank for the NW parasite. Multilocus microsatellite typing was applied in a survey of 98 L. infantum isolates from different NW foci. The microsatellite profiles obtained were compared to those of 308 L. infantum and 20 L. donovani strains from OW countries previously assigned to well-defined populations. Two main populations were identified for both NW and OW L. infantum. Most of the NW strains belonged to population 1, which corresponded to the OW MON-1 population. However, the NW population was much more homogeneous. A second, more heterogeneous, population comprised most Caribbean strains and corresponded to the OW non-MON-1 population. All Brazilian L. infantum strains belonged to population 1, although they represented 61% of the sample and originated from 9 states. Population analysis including the OW L. infantum populations indicated that the NW strains were more similar to MON-1 and non-MON-1 sub-populations of L. infantum from southwest Europe, than to any other OW sub-population. Moreover, similarity between NW and Southwest European L. infantum was higher than between OW L. infantum from distinct parts of the Mediterranean region, Middle East and Central Asia. No correlation was found between NW L. infantum genotypes and clinical picture or host background. This study represents the first continent-wide analysis of NW L. infantum population structure. It confirmed that the agent of VL in the NW is L. infantum and that the parasite has been recently imported multiple times to the NW from southwest Europe.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0001155</identifier><identifier>PMID: 21666787</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Americas - epidemiology ; Biology ; Cluster Analysis ; Evolution, Molecular ; Genetic aspects ; Genotype ; Genotypes ; Heterogeneity ; Humans ; Identification and classification ; Leishmania ; Leishmania donovani ; Leishmania donovani - classification ; Leishmania donovani - genetics ; Leishmania donovani - isolation & purification ; Leishmania infantum ; Leishmania infantum - classification ; Leishmania infantum - genetics ; Leishmania infantum - isolation & purification ; Leishmaniasis, Visceral - epidemiology ; Leishmaniasis, Visceral - parasitology ; Medical research ; Medicine ; Microsatellite Repeats ; Microsatellites (Genetics) ; Molecular Epidemiology ; Molecular Typing - methods ; Parasites ; Parasitic diseases ; Polymorphism, Genetic ; Population ; Population genetics ; Population structure ; Tropical diseases ; Vector-borne diseases</subject><ispartof>PLoS neglected tropical diseases, 2011-06, Vol.5 (6), p.e1155-e1155</ispartof><rights>COPYRIGHT 2011 Public Library of Science</rights><rights>2011 Kuhls et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Kuhls K, Alam MZ, Cupolillo E, Ferreira GEM, Mauricio IL, et al. (2011) Comparative Microsatellite Typing of New World Leishmania infantum Reveals Low Heterogeneity among Populations and Its Recent Old World Origin. PLoS Negl Trop Dis 5(6): e1155. doi:10.1371/journal.pntd.0001155</rights><rights>Kuhls et al. 2011</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c721t-338d44b124754165c4f7ef33401ca7af8b7680f1044f432617bac738f412cf343</citedby><cites>FETCH-LOGICAL-c721t-338d44b124754165c4f7ef33401ca7af8b7680f1044f432617bac738f412cf343</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110170/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3110170/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,723,776,780,860,881,2096,2915,23845,27901,27902,53766,53768,79342,79343</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/21666787$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kuhls, Katrin</creatorcontrib><creatorcontrib>Alam, Mohammad Zahangir</creatorcontrib><creatorcontrib>Cupolillo, Elisa</creatorcontrib><creatorcontrib>Ferreira, Gabriel Eduardo M</creatorcontrib><creatorcontrib>Mauricio, Isabel L</creatorcontrib><creatorcontrib>Oddone, Rolando</creatorcontrib><creatorcontrib>Feliciangeli, M Dora</creatorcontrib><creatorcontrib>Wirth, Thierry</creatorcontrib><creatorcontrib>Miles, Michael A</creatorcontrib><creatorcontrib>Schönian, Gabriele</creatorcontrib><title>Comparative microsatellite typing of new world leishmania infantum reveals low heterogeneity among populations and its recent old world origin</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Leishmania infantum (syn. L. chagasi) is the causative agent of visceral leishmaniasis (VL) in the New World (NW) with endemic regions extending from southern USA to northern Argentina. The two hypotheses about the origin of VL in the NW suggest (1) recent importation of L. infantum from the Old World (OW), or (2) an indigenous origin and a distinct taxonomic rank for the NW parasite. Multilocus microsatellite typing was applied in a survey of 98 L. infantum isolates from different NW foci. The microsatellite profiles obtained were compared to those of 308 L. infantum and 20 L. donovani strains from OW countries previously assigned to well-defined populations. Two main populations were identified for both NW and OW L. infantum. Most of the NW strains belonged to population 1, which corresponded to the OW MON-1 population. However, the NW population was much more homogeneous. A second, more heterogeneous, population comprised most Caribbean strains and corresponded to the OW non-MON-1 population. All Brazilian L. infantum strains belonged to population 1, although they represented 61% of the sample and originated from 9 states. Population analysis including the OW L. infantum populations indicated that the NW strains were more similar to MON-1 and non-MON-1 sub-populations of L. infantum from southwest Europe, than to any other OW sub-population. Moreover, similarity between NW and Southwest European L. infantum was higher than between OW L. infantum from distinct parts of the Mediterranean region, Middle East and Central Asia. No correlation was found between NW L. infantum genotypes and clinical picture or host background. This study represents the first continent-wide analysis of NW L. infantum population structure. It confirmed that the agent of VL in the NW is L. infantum and that the parasite has been recently imported multiple times to the NW from southwest Europe.</description><subject>Americas - epidemiology</subject><subject>Biology</subject><subject>Cluster Analysis</subject><subject>Evolution, Molecular</subject><subject>Genetic aspects</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Heterogeneity</subject><subject>Humans</subject><subject>Identification and classification</subject><subject>Leishmania</subject><subject>Leishmania donovani</subject><subject>Leishmania donovani - classification</subject><subject>Leishmania donovani - genetics</subject><subject>Leishmania donovani - isolation & purification</subject><subject>Leishmania infantum</subject><subject>Leishmania infantum - classification</subject><subject>Leishmania infantum - genetics</subject><subject>Leishmania infantum - isolation & purification</subject><subject>Leishmaniasis, Visceral - epidemiology</subject><subject>Leishmaniasis, Visceral - parasitology</subject><subject>Medical research</subject><subject>Medicine</subject><subject>Microsatellite Repeats</subject><subject>Microsatellites (Genetics)</subject><subject>Molecular Epidemiology</subject><subject>Molecular Typing - methods</subject><subject>Parasites</subject><subject>Parasitic diseases</subject><subject>Polymorphism, Genetic</subject><subject>Population</subject><subject>Population genetics</subject><subject>Population structure</subject><subject>Tropical diseases</subject><subject>Vector-borne diseases</subject><issn>1935-2735</issn><issn>1935-2727</issn><issn>1935-2735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2011</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>BENPR</sourceid><sourceid>DOA</sourceid><recordid>eNqFk11r2zAUhs3YWLtu_2BsgsF2lUyfln1TKGEfhcJutmsh25KjIEueJCfkT-w3T27SkozC0IWF_L7POTo6pyjeIrhEhKPPGz8FJ-1ydKlbQggRYuxZcYlqwhaYE_b8ZH9RvIpxAyGrWYVeFhcYlWXJK35Z_Fn5YZRBJrNVYDBt8FEmZa1JCqT9aFwPvAZO7cDOB9sBq0xcD9IZCYzT0qVpAEFtlbQRWL8Da5VU8L1yyqQ9kIPPgNGPk80RvItAug6YFLOnVS4Bn5EHsA-mN-518UJnlHpz_F4Vv75--bn6vrj78e12dXO3aDlGaUFI1VHaIEw5o6hkLdVcaUIoRK3kUlcNLyuoEaRUU4JLxBvZclJpinCrCSVXxfsDd7Q-imMpo0C4qrIJcpIVtwdF5-VGjMEMMuyFl0bcH_jQCxmSaa0SFZEKV0qrTta0rknFupK2ZccaWTcl55l1fYw2NYPq5psHac-g53-cWYvebwVBCCIOM-DTERD870nFJAYT2_xM0ik_RVHVNWI5Lv6_kmNKaoZn5Yd_lE-X4ajqZb5pfnKfE2xnprjBJSxrRNGc3_IJVV6dyj3lndImn58ZPp4Y1rl90jp6O903ybmQHoRzZ8ag9GPVEBTzHDxkLeY5EMc5yLZ3pxV_ND00PvkLpkEGzA</recordid><startdate>20110601</startdate><enddate>20110601</enddate><creator>Kuhls, Katrin</creator><creator>Alam, Mohammad Zahangir</creator><creator>Cupolillo, Elisa</creator><creator>Ferreira, Gabriel Eduardo M</creator><creator>Mauricio, Isabel L</creator><creator>Oddone, Rolando</creator><creator>Feliciangeli, M Dora</creator><creator>Wirth, Thierry</creator><creator>Miles, Michael A</creator><creator>Schönian, Gabriele</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7T2</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AEUYN</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20110601</creationdate><title>Comparative microsatellite typing of new world leishmania infantum reveals low heterogeneity among populations and its recent old world origin</title><author>Kuhls, Katrin ; Alam, Mohammad Zahangir ; Cupolillo, Elisa ; Ferreira, Gabriel Eduardo M ; Mauricio, Isabel L ; Oddone, Rolando ; Feliciangeli, M Dora ; Wirth, Thierry ; Miles, Michael A ; Schönian, Gabriele</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c721t-338d44b124754165c4f7ef33401ca7af8b7680f1044f432617bac738f412cf343</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2011</creationdate><topic>Americas - epidemiology</topic><topic>Biology</topic><topic>Cluster Analysis</topic><topic>Evolution, Molecular</topic><topic>Genetic aspects</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Heterogeneity</topic><topic>Humans</topic><topic>Identification and classification</topic><topic>Leishmania</topic><topic>Leishmania donovani</topic><topic>Leishmania donovani - classification</topic><topic>Leishmania donovani - genetics</topic><topic>Leishmania donovani - isolation & purification</topic><topic>Leishmania infantum</topic><topic>Leishmania infantum - classification</topic><topic>Leishmania infantum - genetics</topic><topic>Leishmania infantum - isolation & purification</topic><topic>Leishmaniasis, Visceral - epidemiology</topic><topic>Leishmaniasis, Visceral - parasitology</topic><topic>Medical research</topic><topic>Medicine</topic><topic>Microsatellite Repeats</topic><topic>Microsatellites (Genetics)</topic><topic>Molecular Epidemiology</topic><topic>Molecular Typing - methods</topic><topic>Parasites</topic><topic>Parasitic diseases</topic><topic>Polymorphism, Genetic</topic><topic>Population</topic><topic>Population genetics</topic><topic>Population structure</topic><topic>Tropical diseases</topic><topic>Vector-borne diseases</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kuhls, Katrin</creatorcontrib><creatorcontrib>Alam, Mohammad Zahangir</creatorcontrib><creatorcontrib>Cupolillo, Elisa</creatorcontrib><creatorcontrib>Ferreira, Gabriel Eduardo M</creatorcontrib><creatorcontrib>Mauricio, Isabel L</creatorcontrib><creatorcontrib>Oddone, Rolando</creatorcontrib><creatorcontrib>Feliciangeli, M Dora</creatorcontrib><creatorcontrib>Wirth, Thierry</creatorcontrib><creatorcontrib>Miles, Michael A</creatorcontrib><creatorcontrib>Schönian, Gabriele</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest One Sustainability</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Publicly Available Content Database</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - 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L. chagasi) is the causative agent of visceral leishmaniasis (VL) in the New World (NW) with endemic regions extending from southern USA to northern Argentina. The two hypotheses about the origin of VL in the NW suggest (1) recent importation of L. infantum from the Old World (OW), or (2) an indigenous origin and a distinct taxonomic rank for the NW parasite. Multilocus microsatellite typing was applied in a survey of 98 L. infantum isolates from different NW foci. The microsatellite profiles obtained were compared to those of 308 L. infantum and 20 L. donovani strains from OW countries previously assigned to well-defined populations. Two main populations were identified for both NW and OW L. infantum. Most of the NW strains belonged to population 1, which corresponded to the OW MON-1 population. However, the NW population was much more homogeneous. A second, more heterogeneous, population comprised most Caribbean strains and corresponded to the OW non-MON-1 population. All Brazilian L. infantum strains belonged to population 1, although they represented 61% of the sample and originated from 9 states. Population analysis including the OW L. infantum populations indicated that the NW strains were more similar to MON-1 and non-MON-1 sub-populations of L. infantum from southwest Europe, than to any other OW sub-population. Moreover, similarity between NW and Southwest European L. infantum was higher than between OW L. infantum from distinct parts of the Mediterranean region, Middle East and Central Asia. No correlation was found between NW L. infantum genotypes and clinical picture or host background. This study represents the first continent-wide analysis of NW L. infantum population structure. It confirmed that the agent of VL in the NW is L. infantum and that the parasite has been recently imported multiple times to the NW from southwest Europe.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>21666787</pmid><doi>10.1371/journal.pntd.0001155</doi><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1935-2735 |
| ispartof | PLoS neglected tropical diseases, 2011-06, Vol.5 (6), p.e1155-e1155 |
| issn | 1935-2735 1935-2727 1935-2735 |
| language | eng |
| recordid | cdi_plos_journals_1288104073 |
| source | Public Library of Science (PLoS) Journals Open Access; MEDLINE; DOAJ Directory of Open Access Journals; EZB-FREE-00999 freely available EZB journals; PubMed Central; PubMed Central Open Access |
| subjects | Americas - epidemiology Biology Cluster Analysis Evolution, Molecular Genetic aspects Genotype Genotypes Heterogeneity Humans Identification and classification Leishmania Leishmania donovani Leishmania donovani - classification Leishmania donovani - genetics Leishmania donovani - isolation & purification Leishmania infantum Leishmania infantum - classification Leishmania infantum - genetics Leishmania infantum - isolation & purification Leishmaniasis, Visceral - epidemiology Leishmaniasis, Visceral - parasitology Medical research Medicine Microsatellite Repeats Microsatellites (Genetics) Molecular Epidemiology Molecular Typing - methods Parasites Parasitic diseases Polymorphism, Genetic Population Population genetics Population structure Tropical diseases Vector-borne diseases |
| title | Comparative microsatellite typing of new world leishmania infantum reveals low heterogeneity among populations and its recent old world origin |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-05T15%3A31%3A35IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-gale_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Comparative%20microsatellite%20typing%20of%20new%20world%20leishmania%20infantum%20reveals%20low%20heterogeneity%20among%20populations%20and%20its%20recent%20old%20world%20origin&rft.jtitle=PLoS%20neglected%20tropical%20diseases&rft.au=Kuhls,%20Katrin&rft.date=2011-06-01&rft.volume=5&rft.issue=6&rft.spage=e1155&rft.epage=e1155&rft.pages=e1155-e1155&rft.issn=1935-2735&rft.eissn=1935-2735&rft_id=info:doi/10.1371/journal.pntd.0001155&rft_dat=%3Cgale_plos_%3EA260691410%3C/gale_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1288104073&rft_id=info:pmid/21666787&rft_galeid=A260691410&rft_doaj_id=oai_doaj_org_article_83ae28efeda9499385d64c6d5ba9b677&rfr_iscdi=true |