An oral recombinant vaccine in dogs against Echinococcus granulosus, the causative agent of human hydatid disease: a pilot study
Dogs are the main source of human cystic echinococcosis. An oral vaccine would be an important contribution to control programs in endemic countries. We conducted two parallel experimental trials in Morocco and Tunisia of a new oral vaccine candidate against Echinococcus granulosus in 28 dogs. The v...
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| Veröffentlicht in: | PLoS neglected tropical diseases 2008-01, Vol.2 (1), p.e125-e125 |
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| creator | Petavy, Anne-Francoise Hormaeche, Carlos Lahmar, Samia Ouhelli, Hammou Chabalgoity, Alejandro Marchal, Thierry Azzouz, Samira Schreiber, Fernanda Alvite, Gabriela Sarciron, Marie-Elisabeth Maskell, Duncan Esteves, Adriana Bosquet, Georges |
| description | Dogs are the main source of human cystic echinococcosis. An oral vaccine would be an important contribution to control programs in endemic countries. We conducted two parallel experimental trials in Morocco and Tunisia of a new oral vaccine candidate against Echinococcus granulosus in 28 dogs. The vaccine was prepared using two recombinant proteins from adult worms, a tropomyosin (EgTrp) and a fibrillar protein similar to paramyosin (EgA31), cloned and expressed in a live attenuated strain of Salmonella enterica serovar typhimurium.In each country, five dogs were vaccinated with the associated EgA31 and EgTrp; three dogs received only the vector Salmonella; and six dogs were used as different controls. The vaccinated dogs received two oral doses of the vaccine 21 d apart, and were challenged 20 d later with 75,000 living protoscoleces. The controls were challenged under the same conditions. All dogs were sacrificed 26-29 d postchallenge, before the appearance of eggs, for safety reasons.We studied the histological responses to both the vaccine and control at the level of the duodenum, the natural localization of the cestode. Here we show a significant decrease of parasite burden in vaccinated dogs (70% to 80%) and a slower development rate in all remaining worms. The Salmonella vaccine EgA31-EgTrp demonstrated a high efficacy against E. granulosus promoting its potential role in reducing transmission to humans and animals. |
| doi_str_mv | 10.1371/journal.pntd.0000125 |
| format | Article |
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An oral vaccine would be an important contribution to control programs in endemic countries. We conducted two parallel experimental trials in Morocco and Tunisia of a new oral vaccine candidate against Echinococcus granulosus in 28 dogs. The vaccine was prepared using two recombinant proteins from adult worms, a tropomyosin (EgTrp) and a fibrillar protein similar to paramyosin (EgA31), cloned and expressed in a live attenuated strain of Salmonella enterica serovar typhimurium.In each country, five dogs were vaccinated with the associated EgA31 and EgTrp; three dogs received only the vector Salmonella; and six dogs were used as different controls. The vaccinated dogs received two oral doses of the vaccine 21 d apart, and were challenged 20 d later with 75,000 living protoscoleces. The controls were challenged under the same conditions. All dogs were sacrificed 26-29 d postchallenge, before the appearance of eggs, for safety reasons.We studied the histological responses to both the vaccine and control at the level of the duodenum, the natural localization of the cestode. Here we show a significant decrease of parasite burden in vaccinated dogs (70% to 80%) and a slower development rate in all remaining worms. The Salmonella vaccine EgA31-EgTrp demonstrated a high efficacy against E. granulosus promoting its potential role in reducing transmission to humans and animals.</description><identifier>ISSN: 1935-2735</identifier><identifier>ISSN: 1935-2727</identifier><identifier>EISSN: 1935-2735</identifier><identifier>DOI: 10.1371/journal.pntd.0000125</identifier><identifier>PMID: 18235847</identifier><language>eng</language><publisher>United States: Public Library of Science</publisher><subject>Animals ; Antigens, Helminth - genetics ; Antigens, Helminth - immunology ; Antigens, Helminth - metabolism ; Developing countries ; Dogs ; Echinococcosis - immunology ; Echinococcosis - parasitology ; Echinococcosis - prevention & control ; Echinococcus granulosus - genetics ; Echinococcus granulosus - immunology ; Echinococcus granulosus - pathogenicity ; Enzyme-Linked Immunosorbent Assay ; Experiments ; Female ; Humans ; Immunoglobulin A - immunology ; Immunoglobulin E - immunology ; Immunoglobulin G - immunology ; Immunology/Immune Response ; In Vitro Techniques ; Intestinal Mucosa - metabolism ; Intestines - parasitology ; Intestines - ultrastructure ; LDCs ; Livestock ; Lymphocytes ; Male ; Microscopy, Electron, Transmission ; Morocco ; Parasites ; Proteins ; Public health ; Public Health and Epidemiology/Environmental Health ; Recombinant Proteins - genetics ; Recombinant Proteins - immunology ; Recombinant Proteins - metabolism ; Salmonella ; Salmonella typhimurium - genetics ; Salmonella typhimurium - metabolism ; Salmonella Vaccines - immunology ; Sheep ; Tropical diseases ; Tropomyosin - genetics ; Tropomyosin - immunology ; Tropomyosin - metabolism ; Tunisia ; Vaccines ; Vaccines - administration & dosage ; Vaccines - biosynthesis ; Vaccines - genetics ; Vaccines - immunology ; Vaccines, Synthetic - administration & dosage ; Vaccines, Synthetic - biosynthesis ; Vaccines, Synthetic - genetics ; Vaccines, Synthetic - immunology ; Worms</subject><ispartof>PLoS neglected tropical diseases, 2008-01, Vol.2 (1), p.e125-e125</ispartof><rights>2008 Petavy et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited: Petavy A-F, Hormaeche C, Lahmar S, Ouhelli H, Chabalgoity A, et al. (2008) An Oral Recombinant Vaccine in Dogs against Echinococcus granulosus, the Causative Agent of Human Hydatid Disease: A Pilot Study. PLoS Negl Trop Dis 2(1): e125. doi:10.1371/journal.pntd.0000125</rights><rights>Petavy et al. 2008</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c591t-b1d799e6646a1eb57faff6d92794c925dc46a28640aca936f8fadcd0734854a43</citedby><cites>FETCH-LOGICAL-c591t-b1d799e6646a1eb57faff6d92794c925dc46a28640aca936f8fadcd0734854a43</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2217674/pdf/$$EPDF$$P50$$Gpubmedcentral$$Hfree_for_read</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC2217674/$$EHTML$$P50$$Gpubmedcentral$$Hfree_for_read</linktohtml><link.rule.ids>230,314,727,780,784,864,885,2102,2928,23866,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/18235847$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><contributor>Fujiwara, Ricardo</contributor><creatorcontrib>Petavy, Anne-Francoise</creatorcontrib><creatorcontrib>Hormaeche, Carlos</creatorcontrib><creatorcontrib>Lahmar, Samia</creatorcontrib><creatorcontrib>Ouhelli, Hammou</creatorcontrib><creatorcontrib>Chabalgoity, Alejandro</creatorcontrib><creatorcontrib>Marchal, Thierry</creatorcontrib><creatorcontrib>Azzouz, Samira</creatorcontrib><creatorcontrib>Schreiber, Fernanda</creatorcontrib><creatorcontrib>Alvite, Gabriela</creatorcontrib><creatorcontrib>Sarciron, Marie-Elisabeth</creatorcontrib><creatorcontrib>Maskell, Duncan</creatorcontrib><creatorcontrib>Esteves, Adriana</creatorcontrib><creatorcontrib>Bosquet, Georges</creatorcontrib><title>An oral recombinant vaccine in dogs against Echinococcus granulosus, the causative agent of human hydatid disease: a pilot study</title><title>PLoS neglected tropical diseases</title><addtitle>PLoS Negl Trop Dis</addtitle><description>Dogs are the main source of human cystic echinococcosis. An oral vaccine would be an important contribution to control programs in endemic countries. We conducted two parallel experimental trials in Morocco and Tunisia of a new oral vaccine candidate against Echinococcus granulosus in 28 dogs. The vaccine was prepared using two recombinant proteins from adult worms, a tropomyosin (EgTrp) and a fibrillar protein similar to paramyosin (EgA31), cloned and expressed in a live attenuated strain of Salmonella enterica serovar typhimurium.In each country, five dogs were vaccinated with the associated EgA31 and EgTrp; three dogs received only the vector Salmonella; and six dogs were used as different controls. The vaccinated dogs received two oral doses of the vaccine 21 d apart, and were challenged 20 d later with 75,000 living protoscoleces. The controls were challenged under the same conditions. All dogs were sacrificed 26-29 d postchallenge, before the appearance of eggs, for safety reasons.We studied the histological responses to both the vaccine and control at the level of the duodenum, the natural localization of the cestode. Here we show a significant decrease of parasite burden in vaccinated dogs (70% to 80%) and a slower development rate in all remaining worms. The Salmonella vaccine EgA31-EgTrp demonstrated a high efficacy against E. granulosus promoting its potential role in reducing transmission to humans and animals.</description><subject>Animals</subject><subject>Antigens, Helminth - genetics</subject><subject>Antigens, Helminth - immunology</subject><subject>Antigens, Helminth - metabolism</subject><subject>Developing countries</subject><subject>Dogs</subject><subject>Echinococcosis - immunology</subject><subject>Echinococcosis - parasitology</subject><subject>Echinococcosis - prevention & control</subject><subject>Echinococcus granulosus - genetics</subject><subject>Echinococcus granulosus - immunology</subject><subject>Echinococcus granulosus - pathogenicity</subject><subject>Enzyme-Linked Immunosorbent Assay</subject><subject>Experiments</subject><subject>Female</subject><subject>Humans</subject><subject>Immunoglobulin A - immunology</subject><subject>Immunoglobulin E - immunology</subject><subject>Immunoglobulin G - immunology</subject><subject>Immunology/Immune Response</subject><subject>In Vitro Techniques</subject><subject>Intestinal Mucosa - metabolism</subject><subject>Intestines - parasitology</subject><subject>Intestines - ultrastructure</subject><subject>LDCs</subject><subject>Livestock</subject><subject>Lymphocytes</subject><subject>Male</subject><subject>Microscopy, Electron, Transmission</subject><subject>Morocco</subject><subject>Parasites</subject><subject>Proteins</subject><subject>Public health</subject><subject>Public Health and Epidemiology/Environmental Health</subject><subject>Recombinant Proteins - genetics</subject><subject>Recombinant Proteins - immunology</subject><subject>Recombinant Proteins - metabolism</subject><subject>Salmonella</subject><subject>Salmonella typhimurium - genetics</subject><subject>Salmonella typhimurium - metabolism</subject><subject>Salmonella Vaccines - immunology</subject><subject>Sheep</subject><subject>Tropical diseases</subject><subject>Tropomyosin - genetics</subject><subject>Tropomyosin - immunology</subject><subject>Tropomyosin - metabolism</subject><subject>Tunisia</subject><subject>Vaccines</subject><subject>Vaccines - administration & dosage</subject><subject>Vaccines - biosynthesis</subject><subject>Vaccines - genetics</subject><subject>Vaccines - immunology</subject><subject>Vaccines, Synthetic - administration & dosage</subject><subject>Vaccines, Synthetic - biosynthesis</subject><subject>Vaccines, Synthetic - genetics</subject><subject>Vaccines, Synthetic - immunology</subject><subject>Worms</subject><issn>1935-2735</issn><issn>1935-2727</issn><issn>1935-2735</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2008</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><sourceid>ABUWG</sourceid><sourceid>AFKRA</sourceid><sourceid>AZQEC</sourceid><sourceid>BENPR</sourceid><sourceid>CCPQU</sourceid><sourceid>DWQXO</sourceid><sourceid>DOA</sourceid><recordid>eNptkk1v1DAQhiMEoqXwDxBY4sCFXeKv2O4BqaoKVKrEBc7WxHYSr7L2Yicr7Y2fjpcN0CJ8sTXzzOuZ0VtVL3G9xlTg95s4pwDjehcmu67LwYQ_qs6xonxFBOWP773Pqmc5b-qaKy7x0-oMS0K5ZOK8-nEVUEwwouRM3LY-QJjQHozxwSEfkI19RtCDD3lCN2bwIZpozJxRnyDMY8xzfoemwSEDc4bJ713BXRGJHRrmLQQ0HGyJW2R9dpDdJQK082OcUJ5me3hePelgzO7Fcl9U3z7efL3-vLr78un2-upuZbjC06rFVijlmoY1gF3LRQdd11hFhGJGEW5NSRDZsBoMKNp0sgNrbC0ok5wBoxfV65PurvSsl-VljYmUtZKM14W4PRE2wkbvkt9COugIXv8KxNRrSJM3o9Nt13KqjFTCCkZMqyhtqbKStNY0HSNF68Py29xunTVlIWXJD0QfZoIfdB_3mhAsGnFs9-0ikOL32eVJb302bhwhuDhnXeYiNStwId_8Q_5_OHaiTIo5J9f96QXX-uin31X66Ce9-KmUvbo_x9-ixUD0JwnXy4E</recordid><startdate>20080116</startdate><enddate>20080116</enddate><creator>Petavy, Anne-Francoise</creator><creator>Hormaeche, Carlos</creator><creator>Lahmar, Samia</creator><creator>Ouhelli, Hammou</creator><creator>Chabalgoity, Alejandro</creator><creator>Marchal, Thierry</creator><creator>Azzouz, Samira</creator><creator>Schreiber, Fernanda</creator><creator>Alvite, Gabriela</creator><creator>Sarciron, Marie-Elisabeth</creator><creator>Maskell, Duncan</creator><creator>Esteves, Adriana</creator><creator>Bosquet, Georges</creator><general>Public Library of Science</general><general>Public Library of Science (PLoS)</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7SS</scope><scope>7T2</scope><scope>7T7</scope><scope>7U9</scope><scope>7X7</scope><scope>7XB</scope><scope>88E</scope><scope>8C1</scope><scope>8FD</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BENPR</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>F1W</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>H94</scope><scope>H95</scope><scope>H97</scope><scope>K9.</scope><scope>L.G</scope><scope>M0S</scope><scope>M1P</scope><scope>M7N</scope><scope>P64</scope><scope>PIMPY</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>7X8</scope><scope>5PM</scope><scope>DOA</scope></search><sort><creationdate>20080116</creationdate><title>An oral recombinant vaccine in dogs against Echinococcus granulosus, the causative agent of human hydatid disease: a pilot study</title><author>Petavy, Anne-Francoise ; Hormaeche, Carlos ; Lahmar, Samia ; Ouhelli, Hammou ; Chabalgoity, Alejandro ; Marchal, Thierry ; Azzouz, Samira ; Schreiber, Fernanda ; Alvite, Gabriela ; Sarciron, Marie-Elisabeth ; Maskell, Duncan ; Esteves, Adriana ; Bosquet, Georges</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c591t-b1d799e6646a1eb57faff6d92794c925dc46a28640aca936f8fadcd0734854a43</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2008</creationdate><topic>Animals</topic><topic>Antigens, Helminth - 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genetics</topic><topic>Recombinant Proteins - immunology</topic><topic>Recombinant Proteins - metabolism</topic><topic>Salmonella</topic><topic>Salmonella typhimurium - genetics</topic><topic>Salmonella typhimurium - metabolism</topic><topic>Salmonella Vaccines - immunology</topic><topic>Sheep</topic><topic>Tropical diseases</topic><topic>Tropomyosin - genetics</topic><topic>Tropomyosin - immunology</topic><topic>Tropomyosin - metabolism</topic><topic>Tunisia</topic><topic>Vaccines</topic><topic>Vaccines - administration & dosage</topic><topic>Vaccines - biosynthesis</topic><topic>Vaccines - genetics</topic><topic>Vaccines - immunology</topic><topic>Vaccines, Synthetic - administration & dosage</topic><topic>Vaccines, Synthetic - biosynthesis</topic><topic>Vaccines, Synthetic - genetics</topic><topic>Vaccines, Synthetic - immunology</topic><topic>Worms</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Petavy, Anne-Francoise</creatorcontrib><creatorcontrib>Hormaeche, Carlos</creatorcontrib><creatorcontrib>Lahmar, Samia</creatorcontrib><creatorcontrib>Ouhelli, Hammou</creatorcontrib><creatorcontrib>Chabalgoity, Alejandro</creatorcontrib><creatorcontrib>Marchal, Thierry</creatorcontrib><creatorcontrib>Azzouz, Samira</creatorcontrib><creatorcontrib>Schreiber, Fernanda</creatorcontrib><creatorcontrib>Alvite, Gabriela</creatorcontrib><creatorcontrib>Sarciron, Marie-Elisabeth</creatorcontrib><creatorcontrib>Maskell, Duncan</creatorcontrib><creatorcontrib>Esteves, Adriana</creatorcontrib><creatorcontrib>Bosquet, Georges</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>ProQuest Central (Corporate)</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Health and Safety Science Abstracts (Full archive)</collection><collection>Industrial and Applied Microbiology Abstracts (Microbiology A)</collection><collection>Virology and AIDS Abstracts</collection><collection>Health & Medical Collection</collection><collection>ProQuest Central (purchase pre-March 2016)</collection><collection>Medical Database (Alumni Edition)</collection><collection>Public Health Database</collection><collection>Technology Research Database</collection><collection>Hospital Premium Collection</collection><collection>Hospital Premium Collection (Alumni Edition)</collection><collection>ProQuest Central (Alumni) (purchase pre-March 2016)</collection><collection>ProQuest Central (Alumni Edition)</collection><collection>ProQuest Central UK/Ireland</collection><collection>ProQuest Central Essentials</collection><collection>ProQuest Central</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ProQuest One Community College</collection><collection>ProQuest Central Korea</collection><collection>ASFA: Aquatic Sciences and Fisheries Abstracts</collection><collection>Engineering Research Database</collection><collection>Health Research Premium Collection</collection><collection>Health Research Premium Collection (Alumni)</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 1: Biological Sciences & Living Resources</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) 3: Aquatic Pollution & Environmental Quality</collection><collection>ProQuest Health & Medical Complete (Alumni)</collection><collection>Aquatic Science & Fisheries Abstracts (ASFA) Professional</collection><collection>Health & Medical Collection (Alumni Edition)</collection><collection>Medical Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Access via ProQuest (Open Access)</collection><collection>ProQuest One Academic Eastern Edition (DO NOT USE)</collection><collection>ProQuest One Academic</collection><collection>ProQuest One Academic UKI Edition</collection><collection>MEDLINE - 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An oral vaccine would be an important contribution to control programs in endemic countries. We conducted two parallel experimental trials in Morocco and Tunisia of a new oral vaccine candidate against Echinococcus granulosus in 28 dogs. The vaccine was prepared using two recombinant proteins from adult worms, a tropomyosin (EgTrp) and a fibrillar protein similar to paramyosin (EgA31), cloned and expressed in a live attenuated strain of Salmonella enterica serovar typhimurium.In each country, five dogs were vaccinated with the associated EgA31 and EgTrp; three dogs received only the vector Salmonella; and six dogs were used as different controls. The vaccinated dogs received two oral doses of the vaccine 21 d apart, and were challenged 20 d later with 75,000 living protoscoleces. The controls were challenged under the same conditions. All dogs were sacrificed 26-29 d postchallenge, before the appearance of eggs, for safety reasons.We studied the histological responses to both the vaccine and control at the level of the duodenum, the natural localization of the cestode. Here we show a significant decrease of parasite burden in vaccinated dogs (70% to 80%) and a slower development rate in all remaining worms. The Salmonella vaccine EgA31-EgTrp demonstrated a high efficacy against E. granulosus promoting its potential role in reducing transmission to humans and animals.</abstract><cop>United States</cop><pub>Public Library of Science</pub><pmid>18235847</pmid><doi>10.1371/journal.pntd.0000125</doi><oa>free_for_read</oa></addata></record> |
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| recordid | cdi_plos_journals_1288098450 |
| source | MEDLINE; DOAJ Directory of Open Access Journals; PubMed Central Open Access; Public Library of Science (PLoS) Journals Open Access; EZB-FREE-00999 freely available EZB journals; PubMed Central |
| subjects | Animals Antigens, Helminth - genetics Antigens, Helminth - immunology Antigens, Helminth - metabolism Developing countries Dogs Echinococcosis - immunology Echinococcosis - parasitology Echinococcosis - prevention & control Echinococcus granulosus - genetics Echinococcus granulosus - immunology Echinococcus granulosus - pathogenicity Enzyme-Linked Immunosorbent Assay Experiments Female Humans Immunoglobulin A - immunology Immunoglobulin E - immunology Immunoglobulin G - immunology Immunology/Immune Response In Vitro Techniques Intestinal Mucosa - metabolism Intestines - parasitology Intestines - ultrastructure LDCs Livestock Lymphocytes Male Microscopy, Electron, Transmission Morocco Parasites Proteins Public health Public Health and Epidemiology/Environmental Health Recombinant Proteins - genetics Recombinant Proteins - immunology Recombinant Proteins - metabolism Salmonella Salmonella typhimurium - genetics Salmonella typhimurium - metabolism Salmonella Vaccines - immunology Sheep Tropical diseases Tropomyosin - genetics Tropomyosin - immunology Tropomyosin - metabolism Tunisia Vaccines Vaccines - administration & dosage Vaccines - biosynthesis Vaccines - genetics Vaccines - immunology Vaccines, Synthetic - administration & dosage Vaccines, Synthetic - biosynthesis Vaccines, Synthetic - genetics Vaccines, Synthetic - immunology Worms |
| title | An oral recombinant vaccine in dogs against Echinococcus granulosus, the causative agent of human hydatid disease: a pilot study |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-18T17%3A20%3A42IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_plos_&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=An%20oral%20recombinant%20vaccine%20in%20dogs%20against%20Echinococcus%20granulosus,%20the%20causative%20agent%20of%20human%20hydatid%20disease:%20a%20pilot%20study&rft.jtitle=PLoS%20neglected%20tropical%20diseases&rft.au=Petavy,%20Anne-Francoise&rft.date=2008-01-16&rft.volume=2&rft.issue=1&rft.spage=e125&rft.epage=e125&rft.pages=e125-e125&rft.issn=1935-2735&rft.eissn=1935-2735&rft_id=info:doi/10.1371/journal.pntd.0000125&rft_dat=%3Cproquest_plos_%3E734204217%3C/proquest_plos_%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=1288098450&rft_id=info:pmid/18235847&rft_doaj_id=oai_doaj_org_article_bfb539c897d742cb933b39d82bdc6f42&rfr_iscdi=true |