Production of Antibody Associated with non-A, non-B Hepatitis in a Chimpanzee Lymphoblastoid Cell Line Established by in vitro Transformation with Epstein-Barr Virus
A continuous cell line of chimpanzee lymphocytes producing an antibody specifically associated with non-A, non-B hepatitis (NANB) was established. Peripheral blood lymphocytes of a chimpanzee convalescent from experimental infection with NANB hepatitis were transformed in vitro by Epstein-Barr virus...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1985-04, Vol.82 (7), p.2138-2142 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
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| creator | Shimizu, Yohko K. Oomura, Masashi Abe, Kengi Uno, Masatune Yamada, Ei Ono, Yasushi Shikata, Toshio |
| description | A continuous cell line of chimpanzee lymphocytes producing an antibody specifically associated with non-A, non-B hepatitis (NANB) was established. Peripheral blood lymphocytes of a chimpanzee convalescent from experimental infection with NANB hepatitis were transformed in vitro by Epstein-Barr virus infection into lymphoblastoid cell lines. Supernatants of the cell cultures were screened by immunofluorescence for antibody activity against the liver tissue of a chimpanzee with NANB hepatitis. Nineteen of the 1402 cultures were found to be positive for the activity. Ten of these 19 gave cytoplasmic reactions and the remaining 9 gave nuclear reactions in hepatocytes. One culture (48-1) stably producing the antibody was further characterized. The antibody produced in 48-1 was IgM and gave granular cytoplasmic reactions in hepatocytes. Cloning of 48-1 was performed by the soft agar method and cloned cell lines stably producing the antibody were obtained. The 48-1 antibody reacted with liver biopsy specimens from 12 chimpanzees obtained during the acute or chronic phase of hepatitis caused by five different NANB strains, but not with biopsy specimens from chimpanzees with hepatitis A or B or from normal chimpanzees. In addition, examinations of serial liver biopsy specimens obtained from 2 chimpanzees experimentally infected with NANB hepatitis demonstrated that the antibody reacted with the biopsies obtained during the preacute, acute, and chronic hepatitis, but not with those obtained before inoculation, early incubation period, or during convalescence. The present results indicate the specific association of the antibody with NANB hepatitis. Immunoelectron microscopy revealed that the antibody reacted with the microtubular aggregates identical to those previously described in a patient and chimpanzees with NANB hepatitis. |
| doi_str_mv | 10.1073/pnas.82.7.2138 |
| format | Article |
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Peripheral blood lymphocytes of a chimpanzee convalescent from experimental infection with NANB hepatitis were transformed in vitro by Epstein-Barr virus infection into lymphoblastoid cell lines. Supernatants of the cell cultures were screened by immunofluorescence for antibody activity against the liver tissue of a chimpanzee with NANB hepatitis. Nineteen of the 1402 cultures were found to be positive for the activity. Ten of these 19 gave cytoplasmic reactions and the remaining 9 gave nuclear reactions in hepatocytes. One culture (48-1) stably producing the antibody was further characterized. The antibody produced in 48-1 was IgM and gave granular cytoplasmic reactions in hepatocytes. Cloning of 48-1 was performed by the soft agar method and cloned cell lines stably producing the antibody were obtained. The 48-1 antibody reacted with liver biopsy specimens from 12 chimpanzees obtained during the acute or chronic phase of hepatitis caused by five different NANB strains, but not with biopsy specimens from chimpanzees with hepatitis A or B or from normal chimpanzees. In addition, examinations of serial liver biopsy specimens obtained from 2 chimpanzees experimentally infected with NANB hepatitis demonstrated that the antibody reacted with the biopsies obtained during the preacute, acute, and chronic hepatitis, but not with those obtained before inoculation, early incubation period, or during convalescence. The present results indicate the specific association of the antibody with NANB hepatitis. Immunoelectron microscopy revealed that the antibody reacted with the microtubular aggregates identical to those previously described in a patient and chimpanzees with NANB hepatitis.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.82.7.2138</identifier><identifier>PMID: 2984683</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Animal cells ; Animals ; Antibodies ; Antibodies, Viral - analysis ; Biological and medical sciences ; Biopsies ; Biotechnology ; Cell Line ; Cell Transformation, Viral ; Epstein Barr virus infections ; Epstein-Barr virus ; Establishment of new cell lines, improvement of cultural methods, mass cultures ; Eukaryotic cell cultures ; Fundamental and applied biological sciences. Psychology ; Hepatitis ; Hepatitis A ; Hepatitis C - immunology ; Hepatitis C - pathology ; Hepatitis, Viral, Human - immunology ; Hepatocytes ; Herpesvirus 4, Human ; Inoculation ; Liver ; Lymphocytes ; Lymphocytes - immunology ; Methods. Procedures. Technologies ; Microbiology ; Pan troglodytes ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Transformed cell line ; Virology</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1985-04, Vol.82 (7), p.2138-2142</ispartof><rights>1985 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4318-81d08f186fba7eabef81600434ffe21df66f8664383ed3647b083e2eadd5da333</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/82/7.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/25051$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/25051$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27923,27924,53790,53792,58016,58249</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=9197703$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2984683$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shimizu, Yohko K.</creatorcontrib><creatorcontrib>Oomura, Masashi</creatorcontrib><creatorcontrib>Abe, Kengi</creatorcontrib><creatorcontrib>Uno, Masatune</creatorcontrib><creatorcontrib>Yamada, Ei</creatorcontrib><creatorcontrib>Ono, Yasushi</creatorcontrib><creatorcontrib>Shikata, Toshio</creatorcontrib><title>Production of Antibody Associated with non-A, non-B Hepatitis in a Chimpanzee Lymphoblastoid Cell Line Established by in vitro Transformation with Epstein-Barr Virus</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>A continuous cell line of chimpanzee lymphocytes producing an antibody specifically associated with non-A, non-B hepatitis (NANB) was established. Peripheral blood lymphocytes of a chimpanzee convalescent from experimental infection with NANB hepatitis were transformed in vitro by Epstein-Barr virus infection into lymphoblastoid cell lines. Supernatants of the cell cultures were screened by immunofluorescence for antibody activity against the liver tissue of a chimpanzee with NANB hepatitis. Nineteen of the 1402 cultures were found to be positive for the activity. Ten of these 19 gave cytoplasmic reactions and the remaining 9 gave nuclear reactions in hepatocytes. One culture (48-1) stably producing the antibody was further characterized. The antibody produced in 48-1 was IgM and gave granular cytoplasmic reactions in hepatocytes. Cloning of 48-1 was performed by the soft agar method and cloned cell lines stably producing the antibody were obtained. The 48-1 antibody reacted with liver biopsy specimens from 12 chimpanzees obtained during the acute or chronic phase of hepatitis caused by five different NANB strains, but not with biopsy specimens from chimpanzees with hepatitis A or B or from normal chimpanzees. In addition, examinations of serial liver biopsy specimens obtained from 2 chimpanzees experimentally infected with NANB hepatitis demonstrated that the antibody reacted with the biopsies obtained during the preacute, acute, and chronic hepatitis, but not with those obtained before inoculation, early incubation period, or during convalescence. The present results indicate the specific association of the antibody with NANB hepatitis. Immunoelectron microscopy revealed that the antibody reacted with the microtubular aggregates identical to those previously described in a patient and chimpanzees with NANB hepatitis.</description><subject>Animal cells</subject><subject>Animals</subject><subject>Antibodies</subject><subject>Antibodies, Viral - analysis</subject><subject>Biological and medical sciences</subject><subject>Biopsies</subject><subject>Biotechnology</subject><subject>Cell Line</subject><subject>Cell Transformation, Viral</subject><subject>Epstein Barr virus infections</subject><subject>Epstein-Barr virus</subject><subject>Establishment of new cell lines, improvement of cultural methods, mass cultures</subject><subject>Eukaryotic cell cultures</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hepatitis</subject><subject>Hepatitis A</subject><subject>Hepatitis C - immunology</subject><subject>Hepatitis C - pathology</subject><subject>Hepatitis, Viral, Human - immunology</subject><subject>Hepatocytes</subject><subject>Herpesvirus 4, Human</subject><subject>Inoculation</subject><subject>Liver</subject><subject>Lymphocytes</subject><subject>Lymphocytes - immunology</subject><subject>Methods. Procedures. Technologies</subject><subject>Microbiology</subject><subject>Pan troglodytes</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Transformed cell line</subject><subject>Virology</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFUsGO0zAUtBBo6RauHJCQfEB7IsWOncQ5cChVYZEqwWHhajmxTb1K7GA7y5b_4T9x2lLKBU7P0sybN-95AHiG0QKjirwerAgLli-qRY4JewBmGNU4K2mNHoIZQnmVMZrTx-AyhFuEUF0wdAEu8prRkpEZ-PnJOzm20TgLnYZLG03j5A4uQ3CtEVFJ-N3ELbTOZstX-_IWXqtBRBNNgMZCAVdb0w_C_lAKbnb9sHVNJ0J0RsKV6jq4MVbBdYii6UzYJsFmN_XdmegdvPHCBu18L_YW9rPWQ4jKpEHCe_jF-DE8AY-06IJ6eqxz8Pnd-mZ1nW0-vv-wWm6ylhLMMoYlYhqzUjeiUqJRmuESIUqo1irHUpelZmVJCSNKkpJWDUqvXAkpCykIIXPw5qA7jE2vZKts9KLjgze98DvuhOF_I9Zs-Vd3x0ldFUlrDq6O_d59G1WIvDehTUcQVrkx8KpEBcpr-l8ipqSok8NEXByIrXcheKVPZjDiUwD4FADOcl7xKQCp4cX5Cif68ccT_vKIi9CKTqf7tyacaDWuqwqRM5lJ_jd6PubqXzjXY9dFdR8T8fmBeJsi4f_YKVCByS8Uut1q</recordid><startdate>19850401</startdate><enddate>19850401</enddate><creator>Shimizu, Yohko K.</creator><creator>Oomura, Masashi</creator><creator>Abe, Kengi</creator><creator>Uno, Masatune</creator><creator>Yamada, Ei</creator><creator>Ono, Yasushi</creator><creator>Shikata, Toshio</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>7U9</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19850401</creationdate><title>Production of Antibody Associated with non-A, non-B Hepatitis in a Chimpanzee Lymphoblastoid Cell Line Established by in vitro Transformation with Epstein-Barr Virus</title><author>Shimizu, Yohko K. ; Oomura, Masashi ; Abe, Kengi ; Uno, Masatune ; Yamada, Ei ; Ono, Yasushi ; Shikata, Toshio</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4318-81d08f186fba7eabef81600434ffe21df66f8664383ed3647b083e2eadd5da333</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animal cells</topic><topic>Animals</topic><topic>Antibodies</topic><topic>Antibodies, Viral - analysis</topic><topic>Biological and medical sciences</topic><topic>Biopsies</topic><topic>Biotechnology</topic><topic>Cell Line</topic><topic>Cell Transformation, Viral</topic><topic>Epstein Barr virus infections</topic><topic>Epstein-Barr virus</topic><topic>Establishment of new cell lines, improvement of cultural methods, mass cultures</topic><topic>Eukaryotic cell cultures</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hepatitis</topic><topic>Hepatitis A</topic><topic>Hepatitis C - immunology</topic><topic>Hepatitis C - pathology</topic><topic>Hepatitis, Viral, Human - immunology</topic><topic>Hepatocytes</topic><topic>Herpesvirus 4, Human</topic><topic>Inoculation</topic><topic>Liver</topic><topic>Lymphocytes</topic><topic>Lymphocytes - immunology</topic><topic>Methods. Procedures. Technologies</topic><topic>Microbiology</topic><topic>Pan troglodytes</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Transformed cell line</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shimizu, Yohko K.</creatorcontrib><creatorcontrib>Oomura, Masashi</creatorcontrib><creatorcontrib>Abe, Kengi</creatorcontrib><creatorcontrib>Uno, Masatune</creatorcontrib><creatorcontrib>Yamada, Ei</creatorcontrib><creatorcontrib>Ono, Yasushi</creatorcontrib><creatorcontrib>Shikata, Toshio</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shimizu, Yohko K.</au><au>Oomura, Masashi</au><au>Abe, Kengi</au><au>Uno, Masatune</au><au>Yamada, Ei</au><au>Ono, Yasushi</au><au>Shikata, Toshio</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Production of Antibody Associated with non-A, non-B Hepatitis in a Chimpanzee Lymphoblastoid Cell Line Established by in vitro Transformation with Epstein-Barr Virus</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1985-04-01</date><risdate>1985</risdate><volume>82</volume><issue>7</issue><spage>2138</spage><epage>2142</epage><pages>2138-2142</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>A continuous cell line of chimpanzee lymphocytes producing an antibody specifically associated with non-A, non-B hepatitis (NANB) was established. Peripheral blood lymphocytes of a chimpanzee convalescent from experimental infection with NANB hepatitis were transformed in vitro by Epstein-Barr virus infection into lymphoblastoid cell lines. Supernatants of the cell cultures were screened by immunofluorescence for antibody activity against the liver tissue of a chimpanzee with NANB hepatitis. Nineteen of the 1402 cultures were found to be positive for the activity. Ten of these 19 gave cytoplasmic reactions and the remaining 9 gave nuclear reactions in hepatocytes. One culture (48-1) stably producing the antibody was further characterized. The antibody produced in 48-1 was IgM and gave granular cytoplasmic reactions in hepatocytes. Cloning of 48-1 was performed by the soft agar method and cloned cell lines stably producing the antibody were obtained. The 48-1 antibody reacted with liver biopsy specimens from 12 chimpanzees obtained during the acute or chronic phase of hepatitis caused by five different NANB strains, but not with biopsy specimens from chimpanzees with hepatitis A or B or from normal chimpanzees. In addition, examinations of serial liver biopsy specimens obtained from 2 chimpanzees experimentally infected with NANB hepatitis demonstrated that the antibody reacted with the biopsies obtained during the preacute, acute, and chronic hepatitis, but not with those obtained before inoculation, early incubation period, or during convalescence. The present results indicate the specific association of the antibody with NANB hepatitis. Immunoelectron microscopy revealed that the antibody reacted with the microtubular aggregates identical to those previously described in a patient and chimpanzees with NANB hepatitis.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>2984683</pmid><doi>10.1073/pnas.82.7.2138</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1985-04, Vol.82 (7), p.2138-2142 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_9197703 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Animal cells Animals Antibodies Antibodies, Viral - analysis Biological and medical sciences Biopsies Biotechnology Cell Line Cell Transformation, Viral Epstein Barr virus infections Epstein-Barr virus Establishment of new cell lines, improvement of cultural methods, mass cultures Eukaryotic cell cultures Fundamental and applied biological sciences. Psychology Hepatitis Hepatitis A Hepatitis C - immunology Hepatitis C - pathology Hepatitis, Viral, Human - immunology Hepatocytes Herpesvirus 4, Human Inoculation Liver Lymphocytes Lymphocytes - immunology Methods. Procedures. Technologies Microbiology Pan troglodytes Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Transformed cell line Virology |
| title | Production of Antibody Associated with non-A, non-B Hepatitis in a Chimpanzee Lymphoblastoid Cell Line Established by in vitro Transformation with Epstein-Barr Virus |
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