Identification of Individual Interferon-Producing Cells by in situ Hybridization

Individual interferon (IFN)-producing cells were identified by hybridization in situ followed by autoradiography. cDNAs corresponding to murine IFN-α and murine IFN-β labeled by nick-translation to high specific activity (2-4 × 108dpm/μ g) with α -35S-labeled dATP were used as probes for hybridizati...

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Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1985-02, Vol.82 (4), p.1136-1140
Hauptverfasser: Zawatzky, Rainer, De Maeyer, Edward, De Maeyer-Guignard, Jaqueline
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Sprache:eng
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