Chemical Synthesis of Oligonucleotides Containing N6-Methyladenine Residues in the GATC Site

To investigate by NMR the effects of adenine methylation, a set of oligodeoxynucleotides has been synthesized by phosphotriester or phosphoramidite procedures on silica‐gel support. The preparation of fully protected 2′‐deoxy‐N6‐methyladenosine 3′‐phosphate 4 and (2′‐deoxy‐N6‐methyladenosin‐3′‐O‐yl)...

Ausführliche Beschreibung

Gespeichert in:

Bibliographische Detailangaben
Veröffentlicht in:	Helvetica chimica acta 1986-07, Vol.69 (5), p.1034-1040
Hauptverfasser:	Guy, André, Molko, Didier, Wagrez, Laurence, Téoule, Robert
Format:	Artikel
Sprache:	eng
Schlagworte:	Biological and medical sciences Biotechnology Carbohydrates. Nucleosides and nucleotides Chemistry Exact sciences and technology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Methods. Procedures. Technologies Nucleosides, nucleotides and oligonucleotides Organic chemistry Preparations and properties Synthetic digonucleotides and genes. Sequencing
Online-Zugang:	Volltext
Tags:	Tag hinzufügen Keine Tags, Fügen Sie den ersten Tag hinzu!

Beschreibung
Zusammenfassung:	To investigate by NMR the effects of adenine methylation, a set of oligodeoxynucleotides has been synthesized by phosphotriester or phosphoramidite procedures on silica‐gel support. The preparation of fully protected 2′‐deoxy‐N6‐methyladenosine 3′‐phosphate 4 and (2′‐deoxy‐N6‐methyladenosin‐3′‐O‐yl)(methoxy)morpholinophosphine 5 is described. The large‐scale chemical synthesis, purification by HPLC, and characterization by MS of these molecules containing N6‐methyladenine is reported.
ISSN:	0018-019X 1522-2675
DOI:	10.1002/hlca.19860690511