Two Insulin-Like Growth Factor I Messenger RNAs are Expressed in Human Liver
Through use of a synthetic oligonucleotide probe, human insulin-like growth factor I (IGF-I) cDNA clones were isolated from a liver library. Two types of cDNAs were defined by restriction enzyme analysis and DNA sequencing. Both encode IGF-I precursors of either 195 or 153 amino acids. The two predi...
Gespeichert in:
| Veröffentlicht in: | Proc. Natl. Acad. Sci. U.S.A.; (United States) 1986-01, Vol.83 (1), p.77-81 |
|---|---|
| 1. Verfasser: | |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 81 |
|---|---|
| container_issue | 1 |
| container_start_page | 77 |
| container_title | Proc. Natl. Acad. Sci. U.S.A.; (United States) |
| container_volume | 83 |
| creator | Rotwein, Peter |
| description | Through use of a synthetic oligonucleotide probe, human insulin-like growth factor I (IGF-I) cDNA clones were isolated from a liver library. Two types of cDNAs were defined by restriction enzyme analysis and DNA sequencing. Both encode IGF-I precursors of either 195 or 153 amino acids. The two predicted protein precursors are identical from their amino terminus to a lysine residue 16 codons beyond the IGF-I sequence, and then they diverge. Both cDNAs predict additional unique carboxyl-terminal extension peptides. Since there is only one IGF-I gene in the human genome, the finding of two different cDNAs suggests that alternative RNA processing plays a role in IGF-I gene expression. The functions of the different extension peptides remain to be elucidated. |
| doi_str_mv | 10.1073/pnas.83.1.77 |
| format | Article |
| fullrecord | <record><control><sourceid>jstor_pasca</sourceid><recordid>TN_cdi_pascalfrancis_primary_8677910</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>26800</jstor_id><sourcerecordid>26800</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4837-f7576451429f48181d5dba896c574041ee40550b8b358fc232d81cf69919a0e93</originalsourceid><addsrcrecordid>eNp1kUuLFDEURoMoYzu6c6UIQcSV1d5UUnksXAzDPBpKBRnXIZ1OTWesTtqkah7_fqqophgXrgI5597vwofQWwJLAoJ-3QeTl5IuyVKIZ2hBQJGCMwXP0QKgFIVkJXuJXuV8AwCqknCEjiirKsFhgeqru4hXIfetD0Xt_zh8keJdt8XnxnYx4RX-7nJ24dol_OvHScYmOXx2v0_j7wb7gC_7nQm49rcuvUYvGtNm9-bwHqPf52dXp5dF_fNidXpSF5ZJKopGDNmsIqxUDZNEkk21WRupuK0EA0acY1BVsJZrWsnGlrTcSGIbrhRRBpyix-jbtHffr3duY13okmn1PvmdSQ86Gq__JcFv9XW81bQshWLD_MdpPubO62x95-zWxhCc7TRnpOLAB-nzISTFv73Lnd75bF3bmuBin7XgXAggo_hlEm2KOSfXzIcQ0GNDemxIS6qJFmLQPzw9fpYPlQz804GbbE3bJBOsz7Mmh1RFnmrj8plOIbrp27Zz992gvf-_NtB3E73JQ9kzLrkEoI_s1Lb0</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>76677016</pqid></control><display><type>article</type><title>Two Insulin-Like Growth Factor I Messenger RNAs are Expressed in Human Liver</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>Rotwein, Peter</creator><creatorcontrib>Rotwein, Peter ; Washington Univ. School of Medicine, St. Louis</creatorcontrib><description>Through use of a synthetic oligonucleotide probe, human insulin-like growth factor I (IGF-I) cDNA clones were isolated from a liver library. Two types of cDNAs were defined by restriction enzyme analysis and DNA sequencing. Both encode IGF-I precursors of either 195 or 153 amino acids. The two predicted protein precursors are identical from their amino terminus to a lysine residue 16 codons beyond the IGF-I sequence, and then they diverge. Both cDNAs predict additional unique carboxyl-terminal extension peptides. Since there is only one IGF-I gene in the human genome, the finding of two different cDNAs suggests that alternative RNA processing plays a role in IGF-I gene expression. The functions of the different extension peptides remain to be elucidated.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.83.1.77</identifier><identifier>PMID: 3455760</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>550201 - Biochemistry- Tracer Techniques ; Amino acids ; ANIMAL GROWTH ; ANIMALS ; ATP ; Base Sequence ; BASIC BIOLOGICAL SCIENCES ; BETA DECAY RADIOISOTOPES ; BETA-MINUS DECAY RADIOISOTOPES ; Biological and medical sciences ; BIOLOGY ; Biotechnology ; BODY ; Codons ; Complementary DNA ; DAYS LIVING RADIOISOTOPES ; DIGESTIVE SYSTEM ; DNA ; DNA - genetics ; DNA SEQUENCING ; ENZYMES ; ESTERASES ; Fundamental and applied biological sciences. Psychology ; Genetic engineering ; Genetic technics ; GENETICS ; GLANDS ; GROWTH ; HORMONES ; Humans ; HYDROLASES ; INSULIN ; Insulin-Like Growth Factor I - genetics ; ISOTOPES ; LABELLING ; LIGASES ; LIGHT NUCLEI ; LIVER ; Liver - metabolism ; MAMMALS ; MAN ; MESSENGER-RNA ; Methods. Procedures. Technologies ; NUCLEASES ; NUCLEI ; Nucleic Acid Hybridization ; NUCLEIC ACIDS ; Nucleotide sequences ; NUCLEOTIDES ; ODD-ODD NUCLEI ; ORGANIC COMPOUNDS ; ORGANS ; PEPTIDE HORMONES ; PHOSPHODIESTERASES ; PHOSPHORUS 32 ; PHOSPHORUS ISOTOPES ; PHOSPHORUS-GROUP TRANSFERASES ; PHOSPHOTRANSFERASES ; PITUITARY HORMONES ; PRIMATES ; Protein precursors ; Protein Precursors - genetics ; RADIOISOTOPES ; RECOMBINANT DNA ; RNA ; RNA, Messenger - genetics ; Somatomedins - genetics ; STH ; STRUCTURAL CHEMICAL ANALYSIS ; TRANSFERASES ; VERTEBRATES</subject><ispartof>Proc. Natl. Acad. Sci. U.S.A.; (United States), 1986-01, Vol.83 (1), p.77-81</ispartof><rights>1986 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4837-f7576451429f48181d5dba896c574041ee40550b8b358fc232d81cf69919a0e93</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/83/1.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/26800$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/26800$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,4022,27921,27922,27923,53789,53791,58015,58248</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=8677910$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3455760$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink><backlink>$$Uhttps://www.osti.gov/biblio/6415606$$D View this record in Osti.gov$$Hfree_for_read</backlink></links><search><creatorcontrib>Rotwein, Peter</creatorcontrib><creatorcontrib>Washington Univ. School of Medicine, St. Louis</creatorcontrib><title>Two Insulin-Like Growth Factor I Messenger RNAs are Expressed in Human Liver</title><title>Proc. Natl. Acad. Sci. U.S.A.; (United States)</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Through use of a synthetic oligonucleotide probe, human insulin-like growth factor I (IGF-I) cDNA clones were isolated from a liver library. Two types of cDNAs were defined by restriction enzyme analysis and DNA sequencing. Both encode IGF-I precursors of either 195 or 153 amino acids. The two predicted protein precursors are identical from their amino terminus to a lysine residue 16 codons beyond the IGF-I sequence, and then they diverge. Both cDNAs predict additional unique carboxyl-terminal extension peptides. Since there is only one IGF-I gene in the human genome, the finding of two different cDNAs suggests that alternative RNA processing plays a role in IGF-I gene expression. The functions of the different extension peptides remain to be elucidated.</description><subject>550201 - Biochemistry- Tracer Techniques</subject><subject>Amino acids</subject><subject>ANIMAL GROWTH</subject><subject>ANIMALS</subject><subject>ATP</subject><subject>Base Sequence</subject><subject>BASIC BIOLOGICAL SCIENCES</subject><subject>BETA DECAY RADIOISOTOPES</subject><subject>BETA-MINUS DECAY RADIOISOTOPES</subject><subject>Biological and medical sciences</subject><subject>BIOLOGY</subject><subject>Biotechnology</subject><subject>BODY</subject><subject>Codons</subject><subject>Complementary DNA</subject><subject>DAYS LIVING RADIOISOTOPES</subject><subject>DIGESTIVE SYSTEM</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>DNA SEQUENCING</subject><subject>ENZYMES</subject><subject>ESTERASES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>GENETICS</subject><subject>GLANDS</subject><subject>GROWTH</subject><subject>HORMONES</subject><subject>Humans</subject><subject>HYDROLASES</subject><subject>INSULIN</subject><subject>Insulin-Like Growth Factor I - genetics</subject><subject>ISOTOPES</subject><subject>LABELLING</subject><subject>LIGASES</subject><subject>LIGHT NUCLEI</subject><subject>LIVER</subject><subject>Liver - metabolism</subject><subject>MAMMALS</subject><subject>MAN</subject><subject>MESSENGER-RNA</subject><subject>Methods. Procedures. Technologies</subject><subject>NUCLEASES</subject><subject>NUCLEI</subject><subject>Nucleic Acid Hybridization</subject><subject>NUCLEIC ACIDS</subject><subject>Nucleotide sequences</subject><subject>NUCLEOTIDES</subject><subject>ODD-ODD NUCLEI</subject><subject>ORGANIC COMPOUNDS</subject><subject>ORGANS</subject><subject>PEPTIDE HORMONES</subject><subject>PHOSPHODIESTERASES</subject><subject>PHOSPHORUS 32</subject><subject>PHOSPHORUS ISOTOPES</subject><subject>PHOSPHORUS-GROUP TRANSFERASES</subject><subject>PHOSPHOTRANSFERASES</subject><subject>PITUITARY HORMONES</subject><subject>PRIMATES</subject><subject>Protein precursors</subject><subject>Protein Precursors - genetics</subject><subject>RADIOISOTOPES</subject><subject>RECOMBINANT DNA</subject><subject>RNA</subject><subject>RNA, Messenger - genetics</subject><subject>Somatomedins - genetics</subject><subject>STH</subject><subject>STRUCTURAL CHEMICAL ANALYSIS</subject><subject>TRANSFERASES</subject><subject>VERTEBRATES</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1986</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kUuLFDEURoMoYzu6c6UIQcSV1d5UUnksXAzDPBpKBRnXIZ1OTWesTtqkah7_fqqophgXrgI5597vwofQWwJLAoJ-3QeTl5IuyVKIZ2hBQJGCMwXP0QKgFIVkJXuJXuV8AwCqknCEjiirKsFhgeqru4hXIfetD0Xt_zh8keJdt8XnxnYx4RX-7nJ24dol_OvHScYmOXx2v0_j7wb7gC_7nQm49rcuvUYvGtNm9-bwHqPf52dXp5dF_fNidXpSF5ZJKopGDNmsIqxUDZNEkk21WRupuK0EA0acY1BVsJZrWsnGlrTcSGIbrhRRBpyix-jbtHffr3duY13okmn1PvmdSQ86Gq__JcFv9XW81bQshWLD_MdpPubO62x95-zWxhCc7TRnpOLAB-nzISTFv73Lnd75bF3bmuBin7XgXAggo_hlEm2KOSfXzIcQ0GNDemxIS6qJFmLQPzw9fpYPlQz804GbbE3bJBOsz7Mmh1RFnmrj8plOIbrp27Zz992gvf-_NtB3E73JQ9kzLrkEoI_s1Lb0</recordid><startdate>19860101</startdate><enddate>19860101</enddate><creator>Rotwein, Peter</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>OTOTI</scope><scope>5PM</scope></search><sort><creationdate>19860101</creationdate><title>Two Insulin-Like Growth Factor I Messenger RNAs are Expressed in Human Liver</title><author>Rotwein, Peter</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4837-f7576451429f48181d5dba896c574041ee40550b8b358fc232d81cf69919a0e93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1986</creationdate><topic>550201 - Biochemistry- Tracer Techniques</topic><topic>Amino acids</topic><topic>ANIMAL GROWTH</topic><topic>ANIMALS</topic><topic>ATP</topic><topic>Base Sequence</topic><topic>BASIC BIOLOGICAL SCIENCES</topic><topic>BETA DECAY RADIOISOTOPES</topic><topic>BETA-MINUS DECAY RADIOISOTOPES</topic><topic>Biological and medical sciences</topic><topic>BIOLOGY</topic><topic>Biotechnology</topic><topic>BODY</topic><topic>Codons</topic><topic>Complementary DNA</topic><topic>DAYS LIVING RADIOISOTOPES</topic><topic>DIGESTIVE SYSTEM</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>DNA SEQUENCING</topic><topic>ENZYMES</topic><topic>ESTERASES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>GENETICS</topic><topic>GLANDS</topic><topic>GROWTH</topic><topic>HORMONES</topic><topic>Humans</topic><topic>HYDROLASES</topic><topic>INSULIN</topic><topic>Insulin-Like Growth Factor I - genetics</topic><topic>ISOTOPES</topic><topic>LABELLING</topic><topic>LIGASES</topic><topic>LIGHT NUCLEI</topic><topic>LIVER</topic><topic>Liver - metabolism</topic><topic>MAMMALS</topic><topic>MAN</topic><topic>MESSENGER-RNA</topic><topic>Methods. Procedures. Technologies</topic><topic>NUCLEASES</topic><topic>NUCLEI</topic><topic>Nucleic Acid Hybridization</topic><topic>NUCLEIC ACIDS</topic><topic>Nucleotide sequences</topic><topic>NUCLEOTIDES</topic><topic>ODD-ODD NUCLEI</topic><topic>ORGANIC COMPOUNDS</topic><topic>ORGANS</topic><topic>PEPTIDE HORMONES</topic><topic>PHOSPHODIESTERASES</topic><topic>PHOSPHORUS 32</topic><topic>PHOSPHORUS ISOTOPES</topic><topic>PHOSPHORUS-GROUP TRANSFERASES</topic><topic>PHOSPHOTRANSFERASES</topic><topic>PITUITARY HORMONES</topic><topic>PRIMATES</topic><topic>Protein precursors</topic><topic>Protein Precursors - genetics</topic><topic>RADIOISOTOPES</topic><topic>RECOMBINANT DNA</topic><topic>RNA</topic><topic>RNA, Messenger - genetics</topic><topic>Somatomedins - genetics</topic><topic>STH</topic><topic>STRUCTURAL CHEMICAL ANALYSIS</topic><topic>TRANSFERASES</topic><topic>VERTEBRATES</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Rotwein, Peter</creatorcontrib><creatorcontrib>Washington Univ. School of Medicine, St. Louis</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>OSTI.GOV</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Rotwein, Peter</au><aucorp>Washington Univ. School of Medicine, St. Louis</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Two Insulin-Like Growth Factor I Messenger RNAs are Expressed in Human Liver</atitle><jtitle>Proc. Natl. Acad. Sci. U.S.A.; (United States)</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1986-01-01</date><risdate>1986</risdate><volume>83</volume><issue>1</issue><spage>77</spage><epage>81</epage><pages>77-81</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Through use of a synthetic oligonucleotide probe, human insulin-like growth factor I (IGF-I) cDNA clones were isolated from a liver library. Two types of cDNAs were defined by restriction enzyme analysis and DNA sequencing. Both encode IGF-I precursors of either 195 or 153 amino acids. The two predicted protein precursors are identical from their amino terminus to a lysine residue 16 codons beyond the IGF-I sequence, and then they diverge. Both cDNAs predict additional unique carboxyl-terminal extension peptides. Since there is only one IGF-I gene in the human genome, the finding of two different cDNAs suggests that alternative RNA processing plays a role in IGF-I gene expression. The functions of the different extension peptides remain to be elucidated.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3455760</pmid><doi>10.1073/pnas.83.1.77</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0027-8424 |
| ispartof | Proc. Natl. Acad. Sci. U.S.A.; (United States), 1986-01, Vol.83 (1), p.77-81 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_8677910 |
| source | MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | 550201 - Biochemistry- Tracer Techniques Amino acids ANIMAL GROWTH ANIMALS ATP Base Sequence BASIC BIOLOGICAL SCIENCES BETA DECAY RADIOISOTOPES BETA-MINUS DECAY RADIOISOTOPES Biological and medical sciences BIOLOGY Biotechnology BODY Codons Complementary DNA DAYS LIVING RADIOISOTOPES DIGESTIVE SYSTEM DNA DNA - genetics DNA SEQUENCING ENZYMES ESTERASES Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics GENETICS GLANDS GROWTH HORMONES Humans HYDROLASES INSULIN Insulin-Like Growth Factor I - genetics ISOTOPES LABELLING LIGASES LIGHT NUCLEI LIVER Liver - metabolism MAMMALS MAN MESSENGER-RNA Methods. Procedures. Technologies NUCLEASES NUCLEI Nucleic Acid Hybridization NUCLEIC ACIDS Nucleotide sequences NUCLEOTIDES ODD-ODD NUCLEI ORGANIC COMPOUNDS ORGANS PEPTIDE HORMONES PHOSPHODIESTERASES PHOSPHORUS 32 PHOSPHORUS ISOTOPES PHOSPHORUS-GROUP TRANSFERASES PHOSPHOTRANSFERASES PITUITARY HORMONES PRIMATES Protein precursors Protein Precursors - genetics RADIOISOTOPES RECOMBINANT DNA RNA RNA, Messenger - genetics Somatomedins - genetics STH STRUCTURAL CHEMICAL ANALYSIS TRANSFERASES VERTEBRATES |
| title | Two Insulin-Like Growth Factor I Messenger RNAs are Expressed in Human Liver |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T18%3A14%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Two%20Insulin-Like%20Growth%20Factor%20I%20Messenger%20RNAs%20are%20Expressed%20in%20Human%20Liver&rft.jtitle=Proc.%20Natl.%20Acad.%20Sci.%20U.S.A.;%20(United%20States)&rft.au=Rotwein,%20Peter&rft.aucorp=Washington%20Univ.%20School%20of%20Medicine,%20St.%20Louis&rft.date=1986-01-01&rft.volume=83&rft.issue=1&rft.spage=77&rft.epage=81&rft.pages=77-81&rft.issn=0027-8424&rft.eissn=1091-6490&rft.coden=PNASA6&rft_id=info:doi/10.1073/pnas.83.1.77&rft_dat=%3Cjstor_pasca%3E26800%3C/jstor_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=76677016&rft_id=info:pmid/3455760&rft_jstor_id=26800&rfr_iscdi=true |