On the Nature of the 5-lipoxygenase Reaction in Human Leukocytes: Enzyme Purification and Requirement for Multiple Stimulatory Factors

Arachidonate 5-lipoxygenase was purified 400-fold from homogenates of human peripheral blood leukocytes by a combination of ammonium sulfate fractionation, gel filtration chromatography, and HPLC on anion exchange and hydroxylapatite columns. NaDodSO4/polyacrylamide gel electrophoresis of the purifi...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1985-09, Vol.82 (18), p.6040-6044
Hauptverfasser:	Rouzer, Carol A., Samuelsson, Bengt
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical, structural and metabolic biochemistry Arachidonate Lipoxygenases Biological and medical sciences Cell Fractionation - methods Centrifugation Elution Enzyme Activation Enzymes Enzymes and enzyme inhibitors Ethers Flow velocity Fundamental and applied biological sciences. Psychology Gels Homogenization Humans Leukocytes Leukocytes - enzymology Lipoxygenase - isolation & purification Lipoxygenase - metabolism Microsomes - enzymology Molecular Weight Oxidoreductases Quaternary ammonium compounds Sulfates
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