Viruses in Fungi: Infection of Yeast with the K1 and K2 Killer Viruses

We demonstrate here that yeast killer viruses, previously thought to be transmitted only by cytoplasmic mixing during division, mating, or other induced forms of cell fusion, are capable of extracellular transmission. Viral particles from standard K1 and K2 killer strains were used to inoculate sens...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Proceedings of the National Academy of Sciences - PNAS 1987-06, Vol.84 (12), p.4293-4297
Hauptverfasser: El-Sherbeini, Mohamed, Bostian, Keith A.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
container_end_page 4297
container_issue 12
container_start_page 4293
container_title Proceedings of the National Academy of Sciences - PNAS
container_volume 84
creator El-Sherbeini, Mohamed
Bostian, Keith A.
description We demonstrate here that yeast killer viruses, previously thought to be transmitted only by cytoplasmic mixing during division, mating, or other induced forms of cell fusion, are capable of extracellular transmission. Viral particles from standard K1 and K2 killer strains were used to inoculate sensitive cells of Saccharomyces cerevisiae, rendered competent by spheroplasting, lithium acetate treatment, or by natural mating. Extracellular transmission of the killer viruses was judged by the following criteria and controls. (i) Filter-sterilized virus inocula were shown to be free of viable yeast cells, and host cells treated in the absence of added virus did not yield killer progeny. (ii) Infected clones originating from spheroplasts or lithium acetate-treated cells were shown to possess the genotype of the host strain and the killer phenotype of the infecting virus. (iii) Infected clones derived from complementary mating pairs were found to be wild-type diploids, whose meiotic segregants exhibited 2:2 segregation for unlinked nutritional markers and 4:0 segregation for the killer phenotype. This technique is generally applicable to the study of interactions between yeast viruses and different hosts and suggests that extracellular transmission may be a natural route for the inheritance and dissemination of mycoviruses.
doi_str_mv 10.1073/pnas.84.12.4293
format Article
fullrecord <record><control><sourceid>jstor_pasca</sourceid><recordid>TN_cdi_pascalfrancis_primary_8318759</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><jstor_id>29966</jstor_id><sourcerecordid>29966</sourcerecordid><originalsourceid>FETCH-LOGICAL-c521t-573cd465c9c0890efcad07a8c401808d1d05694a27b95ab275b9a99485c7109b3</originalsourceid><addsrcrecordid>eNqFkc9rFDEUx4Moda2eBUHJQeppti-ZZJIIHqS4bdmCFxU8hUwm002ZzaxJxh__vTPsMNRLPb3A9_N9L1--CL0ksCYgyvNDMGkt2ZrQNaOqfIRWBBQpKqbgMVoBUFFIRtlT9CylOwBQXMIJOinp-JCwQptvPg7JJewD3gzh1r_H16F1Nvs-4L7F351JGf_yeYfzzuEtwSY0eEvx1nedi3i2P0dPWtMl92Kep-jr5tOXi6vi5vPl9cXHm8JySnLBRWkbVnGrLEgFrrWmAWGkZUAkyIY0wCvFDBW14qamgtfKKMUkt2LMVZen6MNx72Go966xLuRoOn2Ifm_iH90br_9Vgt_p2_6nLoGDIKP_bPbH_sfgUtZ7n6zrOhNcPyQtBK-Aquq_IGGCc1pN4PkRtLFPKbp2-QwBPVWkp4q0ZJpQPVU0Ol7fz7Dwcyej_nbWTbKma6MJ1qcFkyWRgqsRezNj0_5FvX_n3YOAboeuy-53HslXR_Iu5T4uKFVqDPgXI0a5MQ</addsrcrecordid><sourcetype>Open Access Repository</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>14755266</pqid></control><display><type>article</type><title>Viruses in Fungi: Infection of Yeast with the K1 and K2 Killer Viruses</title><source>MEDLINE</source><source>JSTOR Archive Collection A-Z Listing</source><source>PubMed Central</source><source>Alma/SFX Local Collection</source><source>Free Full-Text Journals in Chemistry</source><creator>El-Sherbeini, Mohamed ; Bostian, Keith A.</creator><creatorcontrib>El-Sherbeini, Mohamed ; Bostian, Keith A.</creatorcontrib><description>We demonstrate here that yeast killer viruses, previously thought to be transmitted only by cytoplasmic mixing during division, mating, or other induced forms of cell fusion, are capable of extracellular transmission. Viral particles from standard K1 and K2 killer strains were used to inoculate sensitive cells of Saccharomyces cerevisiae, rendered competent by spheroplasting, lithium acetate treatment, or by natural mating. Extracellular transmission of the killer viruses was judged by the following criteria and controls. (i) Filter-sterilized virus inocula were shown to be free of viable yeast cells, and host cells treated in the absence of added virus did not yield killer progeny. (ii) Infected clones originating from spheroplasts or lithium acetate-treated cells were shown to possess the genotype of the host strain and the killer phenotype of the infecting virus. (iii) Infected clones derived from complementary mating pairs were found to be wild-type diploids, whose meiotic segregants exhibited 2:2 segregation for unlinked nutritional markers and 4:0 segregation for the killer phenotype. This technique is generally applicable to the study of interactions between yeast viruses and different hosts and suggests that extracellular transmission may be a natural route for the inheritance and dissemination of mycoviruses.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.84.12.4293</identifier><identifier>PMID: 3295880</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Biological and medical sciences ; Cell separation ; Cell walls ; Crosses, Genetic ; Diploidy ; Double stranded RNA ; Fundamental and applied biological sciences. Psychology ; Genotype ; Genotypes ; Infections ; Microbiology ; Morphology, structure, chemical composition, physicochemical properties ; Phenotype ; Phenotypes ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae - physiology ; Spheroplasts ; Spheroplasts - physiology ; Virology ; Virus Physiological Phenomena ; Viruses ; Viruses - genetics ; Yeasts</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1987-06, Vol.84 (12), p.4293-4297</ispartof><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c521t-573cd465c9c0890efcad07a8c401808d1d05694a27b95ab275b9a99485c7109b3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/84/12.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/29966$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/29966$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,315,729,782,786,805,887,27931,27932,53798,53800,58024,58257</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&amp;idt=8318759$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/3295880$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>El-Sherbeini, Mohamed</creatorcontrib><creatorcontrib>Bostian, Keith A.</creatorcontrib><title>Viruses in Fungi: Infection of Yeast with the K1 and K2 Killer Viruses</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>We demonstrate here that yeast killer viruses, previously thought to be transmitted only by cytoplasmic mixing during division, mating, or other induced forms of cell fusion, are capable of extracellular transmission. Viral particles from standard K1 and K2 killer strains were used to inoculate sensitive cells of Saccharomyces cerevisiae, rendered competent by spheroplasting, lithium acetate treatment, or by natural mating. Extracellular transmission of the killer viruses was judged by the following criteria and controls. (i) Filter-sterilized virus inocula were shown to be free of viable yeast cells, and host cells treated in the absence of added virus did not yield killer progeny. (ii) Infected clones originating from spheroplasts or lithium acetate-treated cells were shown to possess the genotype of the host strain and the killer phenotype of the infecting virus. (iii) Infected clones derived from complementary mating pairs were found to be wild-type diploids, whose meiotic segregants exhibited 2:2 segregation for unlinked nutritional markers and 4:0 segregation for the killer phenotype. This technique is generally applicable to the study of interactions between yeast viruses and different hosts and suggests that extracellular transmission may be a natural route for the inheritance and dissemination of mycoviruses.</description><subject>Biological and medical sciences</subject><subject>Cell separation</subject><subject>Cell walls</subject><subject>Crosses, Genetic</subject><subject>Diploidy</subject><subject>Double stranded RNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Infections</subject><subject>Microbiology</subject><subject>Morphology, structure, chemical composition, physicochemical properties</subject><subject>Phenotype</subject><subject>Phenotypes</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae - physiology</subject><subject>Spheroplasts</subject><subject>Spheroplasts - physiology</subject><subject>Virology</subject><subject>Virus Physiological Phenomena</subject><subject>Viruses</subject><subject>Viruses - genetics</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkc9rFDEUx4Moda2eBUHJQeppti-ZZJIIHqS4bdmCFxU8hUwm002ZzaxJxh__vTPsMNRLPb3A9_N9L1--CL0ksCYgyvNDMGkt2ZrQNaOqfIRWBBQpKqbgMVoBUFFIRtlT9CylOwBQXMIJOinp-JCwQptvPg7JJewD3gzh1r_H16F1Nvs-4L7F351JGf_yeYfzzuEtwSY0eEvx1nedi3i2P0dPWtMl92Kep-jr5tOXi6vi5vPl9cXHm8JySnLBRWkbVnGrLEgFrrWmAWGkZUAkyIY0wCvFDBW14qamgtfKKMUkt2LMVZen6MNx72Go966xLuRoOn2Ifm_iH90br_9Vgt_p2_6nLoGDIKP_bPbH_sfgUtZ7n6zrOhNcPyQtBK-Aquq_IGGCc1pN4PkRtLFPKbp2-QwBPVWkp4q0ZJpQPVU0Ol7fz7Dwcyej_nbWTbKma6MJ1qcFkyWRgqsRezNj0_5FvX_n3YOAboeuy-53HslXR_Iu5T4uKFVqDPgXI0a5MQ</recordid><startdate>19870601</startdate><enddate>19870601</enddate><creator>El-Sherbeini, Mohamed</creator><creator>Bostian, Keith A.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7U9</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19870601</creationdate><title>Viruses in Fungi: Infection of Yeast with the K1 and K2 Killer Viruses</title><author>El-Sherbeini, Mohamed ; Bostian, Keith A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c521t-573cd465c9c0890efcad07a8c401808d1d05694a27b95ab275b9a99485c7109b3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Biological and medical sciences</topic><topic>Cell separation</topic><topic>Cell walls</topic><topic>Crosses, Genetic</topic><topic>Diploidy</topic><topic>Double stranded RNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Infections</topic><topic>Microbiology</topic><topic>Morphology, structure, chemical composition, physicochemical properties</topic><topic>Phenotype</topic><topic>Phenotypes</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae - physiology</topic><topic>Spheroplasts</topic><topic>Spheroplasts - physiology</topic><topic>Virology</topic><topic>Virus Physiological Phenomena</topic><topic>Viruses</topic><topic>Viruses - genetics</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>El-Sherbeini, Mohamed</creatorcontrib><creatorcontrib>Bostian, Keith A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>El-Sherbeini, Mohamed</au><au>Bostian, Keith A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Viruses in Fungi: Infection of Yeast with the K1 and K2 Killer Viruses</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1987-06-01</date><risdate>1987</risdate><volume>84</volume><issue>12</issue><spage>4293</spage><epage>4297</epage><pages>4293-4297</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>We demonstrate here that yeast killer viruses, previously thought to be transmitted only by cytoplasmic mixing during division, mating, or other induced forms of cell fusion, are capable of extracellular transmission. Viral particles from standard K1 and K2 killer strains were used to inoculate sensitive cells of Saccharomyces cerevisiae, rendered competent by spheroplasting, lithium acetate treatment, or by natural mating. Extracellular transmission of the killer viruses was judged by the following criteria and controls. (i) Filter-sterilized virus inocula were shown to be free of viable yeast cells, and host cells treated in the absence of added virus did not yield killer progeny. (ii) Infected clones originating from spheroplasts or lithium acetate-treated cells were shown to possess the genotype of the host strain and the killer phenotype of the infecting virus. (iii) Infected clones derived from complementary mating pairs were found to be wild-type diploids, whose meiotic segregants exhibited 2:2 segregation for unlinked nutritional markers and 4:0 segregation for the killer phenotype. This technique is generally applicable to the study of interactions between yeast viruses and different hosts and suggests that extracellular transmission may be a natural route for the inheritance and dissemination of mycoviruses.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>3295880</pmid><doi>10.1073/pnas.84.12.4293</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record>
fulltext fulltext
identifier ISSN: 0027-8424
ispartof Proceedings of the National Academy of Sciences - PNAS, 1987-06, Vol.84 (12), p.4293-4297
issn 0027-8424
1091-6490
language eng
recordid cdi_pascalfrancis_primary_8318759
source MEDLINE; JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry
subjects Biological and medical sciences
Cell separation
Cell walls
Crosses, Genetic
Diploidy
Double stranded RNA
Fundamental and applied biological sciences. Psychology
Genotype
Genotypes
Infections
Microbiology
Morphology, structure, chemical composition, physicochemical properties
Phenotype
Phenotypes
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - physiology
Spheroplasts
Spheroplasts - physiology
Virology
Virus Physiological Phenomena
Viruses
Viruses - genetics
Yeasts
title Viruses in Fungi: Infection of Yeast with the K1 and K2 Killer Viruses
url https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-06T00%3A30%3A12IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-jstor_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Viruses%20in%20Fungi:%20Infection%20of%20Yeast%20with%20the%20K1%20and%20K2%20Killer%20Viruses&rft.jtitle=Proceedings%20of%20the%20National%20Academy%20of%20Sciences%20-%20PNAS&rft.au=El-Sherbeini,%20Mohamed&rft.date=1987-06-01&rft.volume=84&rft.issue=12&rft.spage=4293&rft.epage=4297&rft.pages=4293-4297&rft.issn=0027-8424&rft.eissn=1091-6490&rft.coden=PNASA6&rft_id=info:doi/10.1073/pnas.84.12.4293&rft_dat=%3Cjstor_pasca%3E29966%3C/jstor_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=14755266&rft_id=info:pmid/3295880&rft_jstor_id=29966&rfr_iscdi=true