High-performance liquid chromatography of amino acids and peptides on silica coated with ammonium tungstophosphate : I. Characteristics of the stationary phase

High-performance liquid chromatography of amino acids and peptides on silica coated with ammonium tungstophosphate : I. Characteristics of the stationary phase

Silica coated with ammonium tungstophosphate is proposed as a stationary phase for high-performance liquid chromatography. The chromatographic characteristics of columns packed with this material were investigated. The retention of compounds containing an NH + 4 group, such as amino acids and peptid...

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Veröffentlicht in:Journal of Chromatography A 1986, Vol.370 (1), p.75-82
Hauptverfasser: Desideri, P.G., Lepri, L., Merlini, L., Checchini, L.
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Sprache:eng
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Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Other chromatographic methods
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container_end_page 82
container_issue 1
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container_title Journal of Chromatography A
container_volume 370
creator Desideri, P.G.
Lepri, L.
Merlini, L.
Checchini, L.
description Silica coated with ammonium tungstophosphate is proposed as a stationary phase for high-performance liquid chromatography. The chromatographic characteristics of columns packed with this material were investigated. The retention of compounds containing an NH + 4 group, such as amino acids and peptides, is determined by an ion-exchange mechanism; the silica coated with ammonium tungstophosphate behaves as a reversed phase in the case of aliphatic and aromatic organic acids when eluting with aqueous buffer solutions. The new stationary phase shows a high selectivity towards glycine and tyrosine oligomers.
doi_str_mv 10.1016/S0021-9673(00)94675-2
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subjects Analytical chemistry
Chemistry
Chromatographic methods and physical methods associated with chromatography
Exact sciences and technology
Other chromatographic methods
title High-performance liquid chromatography of amino acids and peptides on silica coated with ammonium tungstophosphate : I. Characteristics of the stationary phase
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