The Effect of Melittin on Na+ and Rb+ Transport in Cultured Skin Fibroblasts of the Spontaneously Hypertensive Rat
Melittin effect on transport of Na+ and Rb+(K+ analog) was examined in cultured skin fibroblasts originating from the Spontaneously Hypertensive, Wistar Kyoto and Wistar rats. Melittin increased both Na+ (22Na++) uptake and 86Rb+ efflux as well as the activity of the Na+-pump (ouabain sensitive 86Rb...
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| Veröffentlicht in: | Clinical and experimental hypertension (1993) 1985, Vol.A7 (9), p.1283-1299 |
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| container_issue | 9 |
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| container_title | Clinical and experimental hypertension (1993) |
| container_volume | A7 |
| creator | Kino, Minoru Tamura, Hirokazu Hopp, Laszlo Tokushige, Aijiroh Searle, Bernard M. Aviv, Abraham |
| description | Melittin effect on transport of Na+ and Rb+(K+ analog) was examined in cultured skin fibroblasts originating from the Spontaneously Hypertensive, Wistar Kyoto and Wistar rats. Melittin increased both Na+ (22Na++) uptake and 86Rb+ efflux as well as the activity of the Na+-pump (ouabain sensitive 86Rb+ uptake) in all three preparations. The effect of the toxin was maximal at a dose of 160-240ng/105 cells/ml. At this does, fibroblasts of the Spontaneously Hypertensive rat demonstrated the greatest response to melittin with respect to the increase in Na+ and Rb+ fluxes and increase in the intracellular Na+ concentrations. It is concluded that melittin can be utilized as a probe to delineate subtle differences in the cellular regulation of Na+ and K+ in the Spontaneously Hypertensive rat as compared with its normotensive controls. |
| doi_str_mv | 10.3109/10641968509073591 |
| format | Article |
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Melittin increased both Na+ (22Na++) uptake and 86Rb+ efflux as well as the activity of the Na+-pump (ouabain sensitive 86Rb+ uptake) in all three preparations. The effect of the toxin was maximal at a dose of 160-240ng/105 cells/ml. At this does, fibroblasts of the Spontaneously Hypertensive rat demonstrated the greatest response to melittin with respect to the increase in Na+ and Rb+ fluxes and increase in the intracellular Na+ concentrations. It is concluded that melittin can be utilized as a probe to delineate subtle differences in the cellular regulation of Na+ and K+ in the Spontaneously Hypertensive rat as compared with its normotensive controls.</description><identifier>ISSN: 1064-1963</identifier><identifier>ISSN: 0730-0077</identifier><identifier>EISSN: 1525-6006</identifier><identifier>DOI: 10.3109/10641968509073591</identifier><identifier>PMID: 4075546</identifier><identifier>CODEN: CEHADM</identifier><language>eng</language><publisher>Basel: Informa UK Ltd</publisher><subject>Animals ; Arterial hypertension. Arterial hypotension ; Bee Venoms - pharmacology ; Biological and medical sciences ; Biological Transport, Active - drug effects ; Blood and lymphatic vessels ; Cardiology. Vascular system ; Cells, Cultured ; Experimental diseases ; Fibroblasts - metabolism ; genetic hypertension ; Hypertension - metabolism ; Medical sciences ; Melitten - pharmacology ; Ouabain - pharmacology ; passive transport ; pump ; Rats ; Rats, Inbred SHR ; Rats, Inbred Strains ; Rubidium - metabolism ; Skin - metabolism ; Sodium - metabolism</subject><ispartof>Clinical and experimental hypertension (1993), 1985, Vol.A7 (9), p.1283-1299</ispartof><rights>1985 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1985</rights><rights>1987 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><cites>FETCH-LOGICAL-c297t-bb5f6cbd5be66fbc0d967f6913b62b32b60a2b757b1469b207607372f72f5b2e3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/10641968509073591$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/10641968509073591$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,4010,27900,27901,27902,59620,60409,61194,61375</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=7932040$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/4075546$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kino, Minoru</creatorcontrib><creatorcontrib>Tamura, Hirokazu</creatorcontrib><creatorcontrib>Hopp, Laszlo</creatorcontrib><creatorcontrib>Tokushige, Aijiroh</creatorcontrib><creatorcontrib>Searle, Bernard M.</creatorcontrib><creatorcontrib>Aviv, Abraham</creatorcontrib><title>The Effect of Melittin on Na+ and Rb+ Transport in Cultured Skin Fibroblasts of the Spontaneously Hypertensive Rat</title><title>Clinical and experimental hypertension (1993)</title><addtitle>Clin Exp Hypertens A</addtitle><description>Melittin effect on transport of Na+ and Rb+(K+ analog) was examined in cultured skin fibroblasts originating from the Spontaneously Hypertensive, Wistar Kyoto and Wistar rats. Melittin increased both Na+ (22Na++) uptake and 86Rb+ efflux as well as the activity of the Na+-pump (ouabain sensitive 86Rb+ uptake) in all three preparations. The effect of the toxin was maximal at a dose of 160-240ng/105 cells/ml. At this does, fibroblasts of the Spontaneously Hypertensive rat demonstrated the greatest response to melittin with respect to the increase in Na+ and Rb+ fluxes and increase in the intracellular Na+ concentrations. It is concluded that melittin can be utilized as a probe to delineate subtle differences in the cellular regulation of Na+ and K+ in the Spontaneously Hypertensive rat as compared with its normotensive controls.</description><subject>Animals</subject><subject>Arterial hypertension. Arterial hypotension</subject><subject>Bee Venoms - pharmacology</subject><subject>Biological and medical sciences</subject><subject>Biological Transport, Active - drug effects</subject><subject>Blood and lymphatic vessels</subject><subject>Cardiology. Vascular system</subject><subject>Cells, Cultured</subject><subject>Experimental diseases</subject><subject>Fibroblasts - metabolism</subject><subject>genetic hypertension</subject><subject>Hypertension - metabolism</subject><subject>Medical sciences</subject><subject>Melitten - pharmacology</subject><subject>Ouabain - pharmacology</subject><subject>passive transport</subject><subject>pump</subject><subject>Rats</subject><subject>Rats, Inbred SHR</subject><subject>Rats, Inbred Strains</subject><subject>Rubidium - metabolism</subject><subject>Skin - metabolism</subject><subject>Sodium - metabolism</subject><issn>1064-1963</issn><issn>0730-0077</issn><issn>1525-6006</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1985</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kF1rFDEUhoMota3-AC-EXEhvymiSmSQb9KYsrRVahXa9HpLMCTs1m4xJRtl_35RdC1IoBJLwfnDOg9A7Sj62lKhPlIiOKrHgRBHZckVfoEPKGW8EIeJlfVe9qYb2NTrK-Y4Q2gm-OEAHHZGcd-IQpdUa8LlzYAuODl-DH0sZA44Bf9enWIcB35hTvEo65Cmmgqu2nH2ZEwz49lf9XYwmReN1LvmhodS-2ymGogPEOfstvtxOkAqEPP4BfKPLG_TKaZ_h7f4-Rj8vzlfLy-bqx9dvy7OrxjIlS2MMd8KagRsQwhlLBiWkE4q2RjDTMiOIZkZyaepSyjAiRWUgmauHGwbtMTrZ9U4p_p4hl34zZgve7ybrpeCso3RRjXRntCnmnMD1Uxo3Om17SvoHzv0TzjXzfl8-mw0Mj4k92Kp_2Os6W-1d5WfH_GiTqmWkI9X2ZWcbg4tpo__G5Ie-6K2P6V-mfW6Kz__F16B9WVudoL-LcwoV7zM73ANPC6oT</recordid><startdate>1985</startdate><enddate>1985</enddate><creator>Kino, Minoru</creator><creator>Tamura, Hirokazu</creator><creator>Hopp, Laszlo</creator><creator>Tokushige, Aijiroh</creator><creator>Searle, Bernard M.</creator><creator>Aviv, Abraham</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Dekker</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1985</creationdate><title>The Effect of Melittin on Na+ and Rb+ Transport in Cultured Skin Fibroblasts of the Spontaneously Hypertensive Rat</title><author>Kino, Minoru ; Tamura, Hirokazu ; Hopp, Laszlo ; Tokushige, Aijiroh ; Searle, Bernard M. ; Aviv, Abraham</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c297t-bb5f6cbd5be66fbc0d967f6913b62b32b60a2b757b1469b207607372f72f5b2e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1985</creationdate><topic>Animals</topic><topic>Arterial hypertension. Arterial hypotension</topic><topic>Bee Venoms - pharmacology</topic><topic>Biological and medical sciences</topic><topic>Biological Transport, Active - drug effects</topic><topic>Blood and lymphatic vessels</topic><topic>Cardiology. Vascular system</topic><topic>Cells, Cultured</topic><topic>Experimental diseases</topic><topic>Fibroblasts - metabolism</topic><topic>genetic hypertension</topic><topic>Hypertension - metabolism</topic><topic>Medical sciences</topic><topic>Melitten - pharmacology</topic><topic>Ouabain - pharmacology</topic><topic>passive transport</topic><topic>pump</topic><topic>Rats</topic><topic>Rats, Inbred SHR</topic><topic>Rats, Inbred Strains</topic><topic>Rubidium - metabolism</topic><topic>Skin - metabolism</topic><topic>Sodium - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kino, Minoru</creatorcontrib><creatorcontrib>Tamura, Hirokazu</creatorcontrib><creatorcontrib>Hopp, Laszlo</creatorcontrib><creatorcontrib>Tokushige, Aijiroh</creatorcontrib><creatorcontrib>Searle, Bernard M.</creatorcontrib><creatorcontrib>Aviv, Abraham</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Clinical and experimental hypertension (1993)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kino, Minoru</au><au>Tamura, Hirokazu</au><au>Hopp, Laszlo</au><au>Tokushige, Aijiroh</au><au>Searle, Bernard M.</au><au>Aviv, Abraham</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The Effect of Melittin on Na+ and Rb+ Transport in Cultured Skin Fibroblasts of the Spontaneously Hypertensive Rat</atitle><jtitle>Clinical and experimental hypertension (1993)</jtitle><addtitle>Clin Exp Hypertens A</addtitle><date>1985</date><risdate>1985</risdate><volume>A7</volume><issue>9</issue><spage>1283</spage><epage>1299</epage><pages>1283-1299</pages><issn>1064-1963</issn><issn>0730-0077</issn><eissn>1525-6006</eissn><coden>CEHADM</coden><abstract>Melittin effect on transport of Na+ and Rb+(K+ analog) was examined in cultured skin fibroblasts originating from the Spontaneously Hypertensive, Wistar Kyoto and Wistar rats. Melittin increased both Na+ (22Na++) uptake and 86Rb+ efflux as well as the activity of the Na+-pump (ouabain sensitive 86Rb+ uptake) in all three preparations. The effect of the toxin was maximal at a dose of 160-240ng/105 cells/ml. At this does, fibroblasts of the Spontaneously Hypertensive rat demonstrated the greatest response to melittin with respect to the increase in Na+ and Rb+ fluxes and increase in the intracellular Na+ concentrations. It is concluded that melittin can be utilized as a probe to delineate subtle differences in the cellular regulation of Na+ and K+ in the Spontaneously Hypertensive rat as compared with its normotensive controls.</abstract><cop>Basel</cop><cop>Hong Kong</cop><cop>New York, NY</cop><pub>Informa UK Ltd</pub><pmid>4075546</pmid><doi>10.3109/10641968509073591</doi><tpages>17</tpages></addata></record> |
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| ispartof | Clinical and experimental hypertension (1993), 1985, Vol.A7 (9), p.1283-1299 |
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| language | eng |
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| source | MEDLINE; Taylor & Francis Journals Complete |
| subjects | Animals Arterial hypertension. Arterial hypotension Bee Venoms - pharmacology Biological and medical sciences Biological Transport, Active - drug effects Blood and lymphatic vessels Cardiology. Vascular system Cells, Cultured Experimental diseases Fibroblasts - metabolism genetic hypertension Hypertension - metabolism Medical sciences Melitten - pharmacology Ouabain - pharmacology passive transport pump Rats Rats, Inbred SHR Rats, Inbred Strains Rubidium - metabolism Skin - metabolism Sodium - metabolism |
| title | The Effect of Melittin on Na+ and Rb+ Transport in Cultured Skin Fibroblasts of the Spontaneously Hypertensive Rat |
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