Expression of a Set of Synthetic Suppressor tRNAPhe Genes in Saccharomyces cerevisiae
Synthetic ochre and amber tRNA suppressor genes derived from the yeast tRNAGAAPhe sequence have been constructed. They were efficiently transcribed in vitro and expressed in vivo via a synthetic expression cassette. tRNAUUAPhe and tRNAUUAPheΔ IVS (IVS = intervening sequence) are relatively inefficie...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1987-10, Vol.84 (19), p.6815-6819 |
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| container_issue | 19 |
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| container_title | Proceedings of the National Academy of Sciences - PNAS |
| container_volume | 84 |
| creator | Masson, Jean-Michel Meuris, Pierre Grunstein, Michael Abelson, John Miller, Jeffrey H. |
| description | Synthetic ochre and amber tRNA suppressor genes derived from the yeast tRNAGAAPhe sequence have been constructed. They were efficiently transcribed in vitro and expressed in vivo via a synthetic expression cassette. tRNAUUAPhe and tRNAUUAPheΔ IVS (IVS = intervening sequence) are relatively inefficient ochre suppressors. They are toxic to the cell when expressed on a multicopy plasmid, and they do not suppress at all when present as single copies. The intron does not seem to have any effect on suppression. In contrast, the amber suppressor tRNACUAPheΔ IVS is efficient when expressed from a single-copy plasmid, while its efficiency is reduced on a multicopy vector. |
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They were efficiently transcribed in vitro and expressed in vivo via a synthetic expression cassette. tRNAUUAPhe and tRNAUUAPheΔ IVS (IVS = intervening sequence) are relatively inefficient ochre suppressors. They are toxic to the cell when expressed on a multicopy plasmid, and they do not suppress at all when present as single copies. The intron does not seem to have any effect on suppression. In contrast, the amber suppressor tRNACUAPheΔ IVS is efficient when expressed from a single-copy plasmid, while its efficiency is reduced on a multicopy vector.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Anticodon ; Biological and medical sciences ; Biotechnology ; Cell growth ; Fundamental and applied biological sciences. 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They were efficiently transcribed in vitro and expressed in vivo via a synthetic expression cassette. tRNAUUAPhe and tRNAUUAPheΔ IVS (IVS = intervening sequence) are relatively inefficient ochre suppressors. They are toxic to the cell when expressed on a multicopy plasmid, and they do not suppress at all when present as single copies. The intron does not seem to have any effect on suppression. In contrast, the amber suppressor tRNACUAPheΔ IVS is efficient when expressed from a single-copy plasmid, while its efficiency is reduced on a multicopy vector.</description><subject>Anticodon</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cell growth</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Genetic vectors</subject><subject>Introns</subject><subject>Methods. Procedures. Technologies</subject><subject>Molecular cloning</subject><subject>Oligonucleotides</subject><subject>Plasmids</subject><subject>Suppressor genes</subject><subject>Transfer RNA</subject><subject>Yeasts</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1987</creationdate><recordtype>article</recordtype><recordid>eNo9jE1Lw0AURQdRsFb_gKtZuA3MZ2ZmWUqtQlExdV1eJi9kQpuEmSjm31tbcXUv9xzuBZlx5niWK8cuyYwxYTKrhLomNym1jDGnLZuRj9X3EDGl0He0rynQAsffUkzd2OAYPC0-h5PRRzq-vyzeGqRr7DDR0NECvG8g9ofJHwePEb9CCoC35KqGfcK7v5yT7eNqu3zKNq_r5-Vik7XKuowLU1eyNEJZ1JwbAFmVzlclKG2MLhWI2mEOUEmhpEahK86Ztig88wYrOScP59sBkod9HaHzIe2GGA4Qp52Rjlnhjtr9WWvT2Md_LFluuPwBLmdXQA</recordid><startdate>19871001</startdate><enddate>19871001</enddate><creator>Masson, Jean-Michel</creator><creator>Meuris, Pierre</creator><creator>Grunstein, Michael</creator><creator>Abelson, John</creator><creator>Miller, Jeffrey H.</creator><general>National Academy of Sciences of the United States of America</general><scope>IQODW</scope></search><sort><creationdate>19871001</creationdate><title>Expression of a Set of Synthetic Suppressor tRNAPhe Genes in Saccharomyces cerevisiae</title><author>Masson, Jean-Michel ; Meuris, Pierre ; Grunstein, Michael ; Abelson, John ; Miller, Jeffrey H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j489-127fd3b7248e5117aa3db9cdba45775b4a2f9e6aad32435e25d11058e2c0c7ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1987</creationdate><topic>Anticodon</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cell growth</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genetic engineering</topic><topic>Genetic technics</topic><topic>Genetic vectors</topic><topic>Introns</topic><topic>Methods. Procedures. Technologies</topic><topic>Molecular cloning</topic><topic>Oligonucleotides</topic><topic>Plasmids</topic><topic>Suppressor genes</topic><topic>Transfer RNA</topic><topic>Yeasts</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Masson, Jean-Michel</creatorcontrib><creatorcontrib>Meuris, Pierre</creatorcontrib><creatorcontrib>Grunstein, Michael</creatorcontrib><creatorcontrib>Abelson, John</creatorcontrib><creatorcontrib>Miller, Jeffrey H.</creatorcontrib><collection>Pascal-Francis</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Masson, Jean-Michel</au><au>Meuris, Pierre</au><au>Grunstein, Michael</au><au>Abelson, John</au><au>Miller, Jeffrey H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Expression of a Set of Synthetic Suppressor tRNAPhe Genes in Saccharomyces cerevisiae</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><date>1987-10-01</date><risdate>1987</risdate><volume>84</volume><issue>19</issue><spage>6815</spage><epage>6819</epage><pages>6815-6819</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Synthetic ochre and amber tRNA suppressor genes derived from the yeast tRNAGAAPhe sequence have been constructed. They were efficiently transcribed in vitro and expressed in vivo via a synthetic expression cassette. tRNAUUAPhe and tRNAUUAPheΔ IVS (IVS = intervening sequence) are relatively inefficient ochre suppressors. They are toxic to the cell when expressed on a multicopy plasmid, and they do not suppress at all when present as single copies. The intron does not seem to have any effect on suppression. In contrast, the amber suppressor tRNACUAPheΔ IVS is efficient when expressed from a single-copy plasmid, while its efficiency is reduced on a multicopy vector.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0027-8424 |
| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1987-10, Vol.84 (19), p.6815-6819 |
| issn | 0027-8424 1091-6490 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_7390829 |
| source | Jstor Complete Legacy; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Anticodon Biological and medical sciences Biotechnology Cell growth Fundamental and applied biological sciences. Psychology Genes Genetic engineering Genetic technics Genetic vectors Introns Methods. Procedures. Technologies Molecular cloning Oligonucleotides Plasmids Suppressor genes Transfer RNA Yeasts |
| title | Expression of a Set of Synthetic Suppressor tRNAPhe Genes in Saccharomyces cerevisiae |
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