Human Follicular Fluid Adverses Hamster Spermatozoa Motility
To determine the optimal conditions for in vitro spermatozoa vitality, human and hamster spermatozoa were incubated at 37 d C in T6 medium supplemented with different biologic fluids (10% v/v). The fluids tested were human serum (HUS), hamster serum (HAS), and human follicular fluid (HUF). After inc...
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| Veröffentlicht in: | Archives of andrology 1989, Vol.23 (2), p.113-117 |
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| creator | Wetzels, A. Goverde, H. J. M. Bastiaans, L. A. Rolland, R. |
| description | To determine the optimal conditions for in vitro spermatozoa vitality, human and hamster spermatozoa were incubated at 37 d C in T6 medium supplemented with different biologic fluids (10% v/v). The fluids tested were human serum (HUS), hamster serum (HAS), and human follicular fluid (HUF). After incubation the spermatozoa were investigated for their qualitative and quantitative motility. Human spermatozoa maintained a good vitality in all fluids tested (approximately 25% motility after 18-h incubation). The hamster spermatozoa had after an incubation of 4 h a motility of 28.4% in HUS, 14.2% in HAS, and 2.2% in HUF. The quality of the motility was also extremely low in HUF, whereas it was adequate in HUS and in HAS. The presence of species-specific substances in mammalian follicular fluid is discussed. |
| doi_str_mv | 10.3109/01485018908986832 |
| format | Article |
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J. M. ; Bastiaans, L. A. ; Rolland, R.</creator><creatorcontrib>Wetzels, A. ; Goverde, H. J. M. ; Bastiaans, L. A. ; Rolland, R.</creatorcontrib><description>To determine the optimal conditions for in vitro spermatozoa vitality, human and hamster spermatozoa were incubated at 37 d C in T6 medium supplemented with different biologic fluids (10% v/v). The fluids tested were human serum (HUS), hamster serum (HAS), and human follicular fluid (HUF). After incubation the spermatozoa were investigated for their qualitative and quantitative motility. Human spermatozoa maintained a good vitality in all fluids tested (approximately 25% motility after 18-h incubation). The hamster spermatozoa had after an incubation of 4 h a motility of 28.4% in HUS, 14.2% in HAS, and 2.2% in HUF. The quality of the motility was also extremely low in HUF, whereas it was adequate in HUS and in HAS. The presence of species-specific substances in mammalian follicular fluid is discussed.</description><identifier>ISSN: 0148-5016</identifier><identifier>EISSN: 1521-0375</identifier><identifier>DOI: 10.3109/01485018908986832</identifier><identifier>PMID: 2589906</identifier><identifier>CODEN: ARANDR</identifier><language>eng</language><publisher>Philadelphia, PA: Informa UK Ltd</publisher><subject>Animals ; Biological and medical sciences ; Blood Physiological Phenomena ; Cricetinae ; Female ; Follicle ; Follicular Fluid - physiology ; Fundamental and applied biological sciences. Psychology ; Hamster ; Human ; Humans ; Male ; Mammalian male genital system ; Mesocricetus ; Morphology. 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J. M.</creatorcontrib><creatorcontrib>Bastiaans, L. A.</creatorcontrib><creatorcontrib>Rolland, R.</creatorcontrib><title>Human Follicular Fluid Adverses Hamster Spermatozoa Motility</title><title>Archives of andrology</title><addtitle>Arch Androl</addtitle><description>To determine the optimal conditions for in vitro spermatozoa vitality, human and hamster spermatozoa were incubated at 37 d C in T6 medium supplemented with different biologic fluids (10% v/v). The fluids tested were human serum (HUS), hamster serum (HAS), and human follicular fluid (HUF). After incubation the spermatozoa were investigated for their qualitative and quantitative motility. Human spermatozoa maintained a good vitality in all fluids tested (approximately 25% motility after 18-h incubation). The hamster spermatozoa had after an incubation of 4 h a motility of 28.4% in HUS, 14.2% in HAS, and 2.2% in HUF. The quality of the motility was also extremely low in HUF, whereas it was adequate in HUS and in HAS. The presence of species-specific substances in mammalian follicular fluid is discussed.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Blood Physiological Phenomena</subject><subject>Cricetinae</subject><subject>Female</subject><subject>Follicle</subject><subject>Follicular Fluid - physiology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Hamster</subject><subject>Human</subject><subject>Humans</subject><subject>Male</subject><subject>Mammalian male genital system</subject><subject>Mesocricetus</subject><subject>Morphology. Physiology</subject><subject>Sperm</subject><subject>Sperm Motility - physiology</subject><subject>Vertebrates: reproduction</subject><issn>0148-5016</issn><issn>1521-0375</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1989</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMFKJDEQhoOsuLPqA3gQ-rB4a610OpkEvQyysyMoHtRzU6Yr2JLujEm3Mj69PcyssAie6vB_f1H1MXbE4VRwMGfASy2BawPaaKVFscMmXBY8BzGVP9hknecjoH6yXyk9A0AhFeyxvUJqY0BN2MViaLHL5sH7xg4eYzb3Q1Nns_qVYqKULbBNPcXsbkmxxT68B8xuQt_4pl8dsF2HPtHhdu6zh_mf-8tFfn379-pydp1bCdDnpaOSlCMNBQrJQTmtTYlagCkea7Bm6pyua7LkQHBJBq0mgWrqtNPCcrHPTjZ7lzG8DJT6qm2SJe-xozCkamqE1NwUI8g3oI0hpUiuWsamxbiqOFRrY9UXY2PneLt8eGyp_mxsFY35722OyaJ3ETvbpE9MGSVKs8YuNljTuTCaegvR11WPKx_iv4747orz_-pPhL5_shipeg5D7Ea93_zwASWdmDg</recordid><startdate>1989</startdate><enddate>1989</enddate><creator>Wetzels, A.</creator><creator>Goverde, H. J. M.</creator><creator>Bastiaans, L. A.</creator><creator>Rolland, R.</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1989</creationdate><title>Human Follicular Fluid Adverses Hamster Spermatozoa Motility</title><author>Wetzels, A. ; Goverde, H. J. M. ; Bastiaans, L. A. ; Rolland, R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c500t-4fe4e6fe802a35106f8894a83092bd0c97ff8ddecef0315e9ac8e3a67f8f83c13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1989</creationdate><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Blood Physiological Phenomena</topic><topic>Cricetinae</topic><topic>Female</topic><topic>Follicle</topic><topic>Follicular Fluid - physiology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Hamster</topic><topic>Human</topic><topic>Humans</topic><topic>Male</topic><topic>Mammalian male genital system</topic><topic>Mesocricetus</topic><topic>Morphology. Physiology</topic><topic>Sperm</topic><topic>Sperm Motility - physiology</topic><topic>Vertebrates: reproduction</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Wetzels, A.</creatorcontrib><creatorcontrib>Goverde, H. J. M.</creatorcontrib><creatorcontrib>Bastiaans, L. A.</creatorcontrib><creatorcontrib>Rolland, R.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of andrology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Wetzels, A.</au><au>Goverde, H. J. M.</au><au>Bastiaans, L. A.</au><au>Rolland, R.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Human Follicular Fluid Adverses Hamster Spermatozoa Motility</atitle><jtitle>Archives of andrology</jtitle><addtitle>Arch Androl</addtitle><date>1989</date><risdate>1989</risdate><volume>23</volume><issue>2</issue><spage>113</spage><epage>117</epage><pages>113-117</pages><issn>0148-5016</issn><eissn>1521-0375</eissn><coden>ARANDR</coden><abstract>To determine the optimal conditions for in vitro spermatozoa vitality, human and hamster spermatozoa were incubated at 37 d C in T6 medium supplemented with different biologic fluids (10% v/v). The fluids tested were human serum (HUS), hamster serum (HAS), and human follicular fluid (HUF). After incubation the spermatozoa were investigated for their qualitative and quantitative motility. Human spermatozoa maintained a good vitality in all fluids tested (approximately 25% motility after 18-h incubation). The hamster spermatozoa had after an incubation of 4 h a motility of 28.4% in HUS, 14.2% in HAS, and 2.2% in HUF. The quality of the motility was also extremely low in HUF, whereas it was adequate in HUS and in HAS. The presence of species-specific substances in mammalian follicular fluid is discussed.</abstract><cop>Philadelphia, PA</cop><pub>Informa UK Ltd</pub><pmid>2589906</pmid><doi>10.3109/01485018908986832</doi><tpages>5</tpages></addata></record> |
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| ispartof | Archives of andrology, 1989, Vol.23 (2), p.113-117 |
| issn | 0148-5016 1521-0375 |
| language | eng |
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| source | MEDLINE; Taylor & Francis Journals; Taylor & Francis Medical Library - CRKN |
| subjects | Animals Biological and medical sciences Blood Physiological Phenomena Cricetinae Female Follicle Follicular Fluid - physiology Fundamental and applied biological sciences. Psychology Hamster Human Humans Male Mammalian male genital system Mesocricetus Morphology. Physiology Sperm Sperm Motility - physiology Vertebrates: reproduction |
| title | Human Follicular Fluid Adverses Hamster Spermatozoa Motility |
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