Interleukin 6: A Functional and Structural in Vitro Modulator of Beta-Cells from Islets of Langerhans
The direct in vitro effect of interleukin-6 (IL-6) on pancreatic beta-cells was studied using isolated Lewis rat islets (25/ml/well) precultured for 7 days and then incubated with or without human recombinant 1L-6 (rIL-6) or purified human natural IL-6 (nlL-6). Both sources of IL-6 stimulated insuli...
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| Veröffentlicht in: | Autoimmunity (Chur, Switzerland) Switzerland), 1990, Vol.5 (3), p.185-194 |
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| creator | Buschard, Karsten Aaen, Kim Horn, Thomas Damme, Jo Van Bendtzen, Klaus |
| description | The direct in vitro effect of interleukin-6 (IL-6) on pancreatic beta-cells was studied using isolated Lewis rat islets (25/ml/well) precultured for 7 days and then incubated with or without human recombinant 1L-6 (rIL-6) or purified human natural IL-6 (nlL-6). Both sources of IL-6 stimulated insulin secretion over a period of 6 days (P < 0.01). whereas the levels of insulin within the islets were unaffected. At concentrations above 1.5ng/ml, rIL-6 almost doubled the content of insulin in the supernatants. At an intermediate concentration, 0.5ng/ml, rIL-6 preserved insulin secretion by islets cocultured with 2ng/ml of human recombinant interleukin lβ (rIL-Iβ) which otherwise inhibited insulin secretion to 60% of islets cultured in medium alone. Electron microscopic studies showed that rIL-6, 1.5ng/ml, caused beta-cell specific degenerative changes similar to those previously described after treatment with IL-1β; i.e. appearance of opaque intracytoplasmic bodies, autophage vacuoles and signs of mitochondrial degeneration. We conclude that human IL-6 stimulates insulin production and secretion in virro and induces similar ultrastructural changes in beta-cells as does IL-Iβ. IL-6 may be an endogenous mediator of some of the effects on beta-cells ascribed to IL-1. |
| doi_str_mv | 10.3109/08916939009002977 |
| format | Article |
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Both sources of IL-6 stimulated insulin secretion over a period of 6 days (P < 0.01). whereas the levels of insulin within the islets were unaffected. At concentrations above 1.5ng/ml, rIL-6 almost doubled the content of insulin in the supernatants. At an intermediate concentration, 0.5ng/ml, rIL-6 preserved insulin secretion by islets cocultured with 2ng/ml of human recombinant interleukin lβ (rIL-Iβ) which otherwise inhibited insulin secretion to 60% of islets cultured in medium alone. Electron microscopic studies showed that rIL-6, 1.5ng/ml, caused beta-cell specific degenerative changes similar to those previously described after treatment with IL-1β; i.e. appearance of opaque intracytoplasmic bodies, autophage vacuoles and signs of mitochondrial degeneration. We conclude that human IL-6 stimulates insulin production and secretion in virro and induces similar ultrastructural changes in beta-cells as does IL-Iβ. IL-6 may be an endogenous mediator of some of the effects on beta-cells ascribed to IL-1.</description><identifier>ISSN: 0891-6934</identifier><identifier>EISSN: 1607-842X</identifier><identifier>DOI: 10.3109/08916939009002977</identifier><identifier>PMID: 2129751</identifier><language>eng</language><publisher>Abingdon: Informa UK Ltd</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Animals ; Biological and medical sciences ; Diabetes Mellitus, Type 1 - etiology ; electron microscopy ; Fundamental and applied biological sciences. Psychology ; Fundamental immunology ; Immunobiology ; In Vitro Techniques ; Insulin - biosynthesis ; Insulin - metabolism ; Insulin Secretion ; insulin-dependent diabetes mellitus ; Interleukin 1 ; interleukin 6 ; Interleukin-1 - pharmacology ; Interleukin-1 - physiology ; Interleukin-6 - pharmacology ; Interleukin-6 - physiology ; Islets of Langerhans - drug effects ; Islets of Langerhans - physiology ; Islets of Langerhans - ultrastructure ; Lymphokines, interleukins ( function, expression) ; Male ; Microscopy, Electron ; pancreatic islets of Langerhans ; Rats ; Rats, Inbred Lew ; Regulatory factors and their cellular receptors ; ultrastructure</subject><ispartof>Autoimmunity (Chur, Switzerland), 1990, Vol.5 (3), p.185-194</ispartof><rights>1990 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1990</rights><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c345t-d38c8000d4ece23b2512b2568c05f61201ca63d5c6c4be51a807f15e110c0d743</citedby><cites>FETCH-LOGICAL-c345t-d38c8000d4ece23b2512b2568c05f61201ca63d5c6c4be51a807f15e110c0d743</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/08916939009002977$$EPDF$$P50$$Ginformahealthcare$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/08916939009002977$$EHTML$$P50$$Ginformahealthcare$$H</linktohtml><link.rule.ids>314,776,780,4010,27900,27901,27902,59620,60409,61194,61375</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6932125$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/2129751$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Buschard, Karsten</creatorcontrib><creatorcontrib>Aaen, Kim</creatorcontrib><creatorcontrib>Horn, Thomas</creatorcontrib><creatorcontrib>Damme, Jo Van</creatorcontrib><creatorcontrib>Bendtzen, Klaus</creatorcontrib><title>Interleukin 6: A Functional and Structural in Vitro Modulator of Beta-Cells from Islets of Langerhans</title><title>Autoimmunity (Chur, Switzerland)</title><addtitle>Autoimmunity</addtitle><description>The direct in vitro effect of interleukin-6 (IL-6) on pancreatic beta-cells was studied using isolated Lewis rat islets (25/ml/well) precultured for 7 days and then incubated with or without human recombinant 1L-6 (rIL-6) or purified human natural IL-6 (nlL-6). Both sources of IL-6 stimulated insulin secretion over a period of 6 days (P < 0.01). whereas the levels of insulin within the islets were unaffected. At concentrations above 1.5ng/ml, rIL-6 almost doubled the content of insulin in the supernatants. At an intermediate concentration, 0.5ng/ml, rIL-6 preserved insulin secretion by islets cocultured with 2ng/ml of human recombinant interleukin lβ (rIL-Iβ) which otherwise inhibited insulin secretion to 60% of islets cultured in medium alone. Electron microscopic studies showed that rIL-6, 1.5ng/ml, caused beta-cell specific degenerative changes similar to those previously described after treatment with IL-1β; i.e. appearance of opaque intracytoplasmic bodies, autophage vacuoles and signs of mitochondrial degeneration. We conclude that human IL-6 stimulates insulin production and secretion in virro and induces similar ultrastructural changes in beta-cells as does IL-Iβ. IL-6 may be an endogenous mediator of some of the effects on beta-cells ascribed to IL-1.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Diabetes Mellitus, Type 1 - etiology</subject><subject>electron microscopy</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Immunobiology</subject><subject>In Vitro Techniques</subject><subject>Insulin - biosynthesis</subject><subject>Insulin - metabolism</subject><subject>Insulin Secretion</subject><subject>insulin-dependent diabetes mellitus</subject><subject>Interleukin 1</subject><subject>interleukin 6</subject><subject>Interleukin-1 - pharmacology</subject><subject>Interleukin-1 - physiology</subject><subject>Interleukin-6 - pharmacology</subject><subject>Interleukin-6 - physiology</subject><subject>Islets of Langerhans - drug effects</subject><subject>Islets of Langerhans - physiology</subject><subject>Islets of Langerhans - ultrastructure</subject><subject>Lymphokines, interleukins ( function, expression)</subject><subject>Male</subject><subject>Microscopy, Electron</subject><subject>pancreatic islets of Langerhans</subject><subject>Rats</subject><subject>Rats, Inbred Lew</subject><subject>Regulatory factors and their cellular receptors</subject><subject>ultrastructure</subject><issn>0891-6934</issn><issn>1607-842X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1990</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kV9rFDEUxYModa1-AB-EPIhvo8nMJDOjfWkXaxdWfPAPvg13M3fcqZmk3iSUfnuz7LZQCoWQEM7vXE5OGHstxftKiu6DaDupu6oTIq-ya5onbCG1aIq2Ln8_ZYudXmSgfs5ehHApMtTo-ogdlTLTSi4YrlxEspj-To7rj_yUnydn4uQdWA5u4N8jJRMT5Wsmfk2RPP_qh2QheuJ-5GcYoViitYGP5Ge-ChZj2ClrcH-QtuDCS_ZsBBvw1eE8Zj_PP_9YXhTrb19Wy9N1YapaxWKoWtPmkEONBstqUypZ5k23RqhRy1JIA7oalNGm3qCS0IpmlAqlFEYMTV0ds3f7uVfk_yUMsZ-nYHI2cOhT6FtRtVqVXQblHjTkQyAc-yuaZqCbXop-V23_oNrseXMYnjYzDneOQ5dZf3vQIRiwI4EzU7jD8rBMqoyd7LHJjZ5muPZkhz7CjfV066keS_Hpnn2LYOPWAGF_6RPlbwuPvOE_Rt2lEw</recordid><startdate>1990</startdate><enddate>1990</enddate><creator>Buschard, Karsten</creator><creator>Aaen, Kim</creator><creator>Horn, Thomas</creator><creator>Damme, Jo Van</creator><creator>Bendtzen, Klaus</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Taylor and Francis</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1990</creationdate><title>Interleukin 6: A Functional and Structural in Vitro Modulator of Beta-Cells from Islets of Langerhans</title><author>Buschard, Karsten ; Aaen, Kim ; Horn, Thomas ; Damme, Jo Van ; Bendtzen, Klaus</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c345t-d38c8000d4ece23b2512b2568c05f61201ca63d5c6c4be51a807f15e110c0d743</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1990</creationdate><topic>Analysis of the immune response. Humoral and cellular immunity</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Diabetes Mellitus, Type 1 - etiology</topic><topic>electron microscopy</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Immunobiology</topic><topic>In Vitro Techniques</topic><topic>Insulin - biosynthesis</topic><topic>Insulin - metabolism</topic><topic>Insulin Secretion</topic><topic>insulin-dependent diabetes mellitus</topic><topic>Interleukin 1</topic><topic>interleukin 6</topic><topic>Interleukin-1 - pharmacology</topic><topic>Interleukin-1 - physiology</topic><topic>Interleukin-6 - pharmacology</topic><topic>Interleukin-6 - physiology</topic><topic>Islets of Langerhans - drug effects</topic><topic>Islets of Langerhans - physiology</topic><topic>Islets of Langerhans - ultrastructure</topic><topic>Lymphokines, interleukins ( function, expression)</topic><topic>Male</topic><topic>Microscopy, Electron</topic><topic>pancreatic islets of Langerhans</topic><topic>Rats</topic><topic>Rats, Inbred Lew</topic><topic>Regulatory factors and their cellular receptors</topic><topic>ultrastructure</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Buschard, Karsten</creatorcontrib><creatorcontrib>Aaen, Kim</creatorcontrib><creatorcontrib>Horn, Thomas</creatorcontrib><creatorcontrib>Damme, Jo Van</creatorcontrib><creatorcontrib>Bendtzen, Klaus</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Autoimmunity (Chur, Switzerland)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Buschard, Karsten</au><au>Aaen, Kim</au><au>Horn, Thomas</au><au>Damme, Jo Van</au><au>Bendtzen, Klaus</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Interleukin 6: A Functional and Structural in Vitro Modulator of Beta-Cells from Islets of Langerhans</atitle><jtitle>Autoimmunity (Chur, Switzerland)</jtitle><addtitle>Autoimmunity</addtitle><date>1990</date><risdate>1990</risdate><volume>5</volume><issue>3</issue><spage>185</spage><epage>194</epage><pages>185-194</pages><issn>0891-6934</issn><eissn>1607-842X</eissn><abstract>The direct in vitro effect of interleukin-6 (IL-6) on pancreatic beta-cells was studied using isolated Lewis rat islets (25/ml/well) precultured for 7 days and then incubated with or without human recombinant 1L-6 (rIL-6) or purified human natural IL-6 (nlL-6). Both sources of IL-6 stimulated insulin secretion over a period of 6 days (P < 0.01). whereas the levels of insulin within the islets were unaffected. At concentrations above 1.5ng/ml, rIL-6 almost doubled the content of insulin in the supernatants. At an intermediate concentration, 0.5ng/ml, rIL-6 preserved insulin secretion by islets cocultured with 2ng/ml of human recombinant interleukin lβ (rIL-Iβ) which otherwise inhibited insulin secretion to 60% of islets cultured in medium alone. Electron microscopic studies showed that rIL-6, 1.5ng/ml, caused beta-cell specific degenerative changes similar to those previously described after treatment with IL-1β; i.e. appearance of opaque intracytoplasmic bodies, autophage vacuoles and signs of mitochondrial degeneration. We conclude that human IL-6 stimulates insulin production and secretion in virro and induces similar ultrastructural changes in beta-cells as does IL-Iβ. IL-6 may be an endogenous mediator of some of the effects on beta-cells ascribed to IL-1.</abstract><cop>Abingdon</cop><pub>Informa UK Ltd</pub><pmid>2129751</pmid><doi>10.3109/08916939009002977</doi><tpages>10</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0891-6934 |
| ispartof | Autoimmunity (Chur, Switzerland), 1990, Vol.5 (3), p.185-194 |
| issn | 0891-6934 1607-842X |
| language | eng |
| recordid | cdi_pascalfrancis_primary_6932125 |
| source | Taylor & Francis:Master (3349 titles); MEDLINE |
| subjects | Analysis of the immune response. Humoral and cellular immunity Animals Biological and medical sciences Diabetes Mellitus, Type 1 - etiology electron microscopy Fundamental and applied biological sciences. Psychology Fundamental immunology Immunobiology In Vitro Techniques Insulin - biosynthesis Insulin - metabolism Insulin Secretion insulin-dependent diabetes mellitus Interleukin 1 interleukin 6 Interleukin-1 - pharmacology Interleukin-1 - physiology Interleukin-6 - pharmacology Interleukin-6 - physiology Islets of Langerhans - drug effects Islets of Langerhans - physiology Islets of Langerhans - ultrastructure Lymphokines, interleukins ( function, expression) Male Microscopy, Electron pancreatic islets of Langerhans Rats Rats, Inbred Lew Regulatory factors and their cellular receptors ultrastructure |
| title | Interleukin 6: A Functional and Structural in Vitro Modulator of Beta-Cells from Islets of Langerhans |
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