A Heterogeneous Double Antibody Enzyme-Linked Immunoassay to Measure β-Galactosidase Fusion Protein

A Heterogeneous Double Antibody Enzyme-Linked Immunoassay to Measure β-Galactosidase Fusion Protein

A rapid and sensitive enzyme-linked immunoassay (ELISA) to quantitate recombinant fusion proteins encoded by cloned cDNA in the bacteriophage λgt111 described. Since the fusion protein is expressed in an equimolar ratio to β-galactosidase, the assay derives the concentration of the recombinant prote...

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Veröffentlicht in:Journal of immunoassay (Monticello, N.Y.) N.Y.), 1990-01, Vol.11 (1), p.89-95
Hauptverfasser: Hayashibe, Kazuhito, Sassano, Daniela, Ziai, M. Reza
Format: Artikel
Sprache:eng
Schlagworte:
beta-Galactosidase - analysis
Biological and medical sciences
ELISA
Enzyme-Linked Immunosorbent Assay
Fundamental and applied biological sciences. Psychology
Fundamental immunology
fusion protein
Galactosidases - analysis
Immunoenzyme Techniques
Molecular immunology
Radioimmunoassay
Recombinant Fusion Proteins - analysis
Techniques
λgt11
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Beschreibung
Zusammenfassung:A rapid and sensitive enzyme-linked immunoassay (ELISA) to quantitate recombinant fusion proteins encoded by cloned cDNA in the bacteriophage λgt111 described. Since the fusion protein is expressed in an equimolar ratio to β-galactosidase, the assay derives the concentration of the recombinant protein in total bacterial lysates or pure preparations from the measurement of β-galactosidase with an enzyme-linked immunoassay. This assay is a useful technique to measure the recombinant proteins for subsequent immunological and biochemical characterization.
ISSN:0197-1522
2332-4090
DOI:10.1080/01971529008053260