Transformation of the cryobehavior of rye protoplasts by modification of the plasma membrane lipid composition
The freezing tolerance of protoplasts isolated from nonacclimated rye leaves (Secale cereale L. cv Puma) was significantly altered by using a pH-induced protoplast-liposome fusion technique to modify the lipid composition of the plasma membrane. The increase in freezing tolerance was elicited by fus...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1988-12, Vol.85 (23), p.9026-9030 |
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| creator | Steponkus, P.L Uemura, M Balsamo, R.A Arvinte, T Lynch, D.V |
| description | The freezing tolerance of protoplasts isolated from nonacclimated rye leaves (Secale cereale L. cv Puma) was significantly altered by using a pH-induced protoplast-liposome fusion technique to modify the lipid composition of the plasma membrane. The increase in freezing tolerance was elicited by fusion with liposomes composed of either the total phospholipid fraction isolated from the plasma membrane of cold-acclimated leaves or single mono- or diunsaturated species of phosphatidylcholine (PtdCho). Of the PtdCho species tested, dilinoleoylphosphatidylcholine ([Lin2]PtdCho) and dilinolenoylphosphatidylcholine ([Lnn2]PtdCho) liposomes were the most effective; 1-palmitoyl-2-oleoylphosphatidylcholine, 1-palmitoyl-2-linoleoylphosphatidylcholine, or dioleoylphosphatidylcholine liposomes were somewhat less effective; dimyristoylphosphatidylcholine or dipalmitoylphosphatidylcholine liposomes had no effect. The increased freezing tolerance was the result of a transformation in the cryobehavior of the plasma membrane during freeze-induced osmotic contraction. In control nonacclimated protoplasts, osmotic contraction resulted in endocytotic vesiculation of the plasma membrane which was irreversible and resulted in lysis during osmotic expansion after melting of the suspending medium. In nonacclimated protoplasts fused with mono- or diunsaturated species of PtdCho, osmotic contraction resulted in the reversible formation of exocytotic extrusions of the plasma membrane--as normally occurs in protoplasts isolated from cold-acclimated leaves (acclimated protoplasts). In scanning electron micrographs, the morphology of the extrusions of nonacclimated protoplasts fused with [Lin2]PtdCho was virtually indistinguishable from that of the extrusions formed in acclimated protoplasts. These studies provide direct evidence that changes in the lipid composition of the plasma membrane are causally related to one facet of the cold-acclimation process. |
| doi_str_mv | 10.1073/pnas.85.23.9026 |
| format | Article |
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The increase in freezing tolerance was elicited by fusion with liposomes composed of either the total phospholipid fraction isolated from the plasma membrane of cold-acclimated leaves or single mono- or diunsaturated species of phosphatidylcholine (PtdCho). Of the PtdCho species tested, dilinoleoylphosphatidylcholine ([Lin2]PtdCho) and dilinolenoylphosphatidylcholine ([Lnn2]PtdCho) liposomes were the most effective; 1-palmitoyl-2-oleoylphosphatidylcholine, 1-palmitoyl-2-linoleoylphosphatidylcholine, or dioleoylphosphatidylcholine liposomes were somewhat less effective; dimyristoylphosphatidylcholine or dipalmitoylphosphatidylcholine liposomes had no effect. The increased freezing tolerance was the result of a transformation in the cryobehavior of the plasma membrane during freeze-induced osmotic contraction. In control nonacclimated protoplasts, osmotic contraction resulted in endocytotic vesiculation of the plasma membrane which was irreversible and resulted in lysis during osmotic expansion after melting of the suspending medium. In nonacclimated protoplasts fused with mono- or diunsaturated species of PtdCho, osmotic contraction resulted in the reversible formation of exocytotic extrusions of the plasma membrane--as normally occurs in protoplasts isolated from cold-acclimated leaves (acclimated protoplasts). In scanning electron micrographs, the morphology of the extrusions of nonacclimated protoplasts fused with [Lin2]PtdCho was virtually indistinguishable from that of the extrusions formed in acclimated protoplasts. These studies provide direct evidence that changes in the lipid composition of the plasma membrane are causally related to one facet of the cold-acclimation process.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.85.23.9026</identifier><identifier>PMID: 16594000</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>Acclimatization ; Biological and medical sciences ; Biological Sciences: Botany ; Cell membranes ; Cell nucleus ; CELL STRUCTURE ; Cell structures and functions ; Chemical composition ; COLD ; CONGELACION ; CONGELATION ; ESTRUCTURA CELULAR ; FEUILLE ; FREEZING ; FRIO ; FROID ; Fundamental and applied biological sciences. Psychology ; HOJAS ; LEAVES ; LIPIDE ; LIPIDOS ; LIPIDS ; Liposomes ; Molecular and cellular biology ; OSMOSE ; OSMOSIS ; Phospholipids ; Plasma physics ; PROTOPLASTE ; PROTOPLASTO ; PROTOPLASTS ; RESISTANCE A LA TEMPERATURE ; RESISTENCIA A LA TEMPERATURA ; Rye ; SECALE CEREALE ; STRUCTURE CELLULAIRE ; TEMPERATURE RESISTANCE</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1988-12, Vol.85 (23), p.9026-9030</ispartof><rights>1990 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c509t-c95ba6088c410bf2d7fe0ce3996c20f6e2cd9cb937d0043a50b4e3a1531043f03</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/85/23.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/32879$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/32879$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=6848632$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/16594000$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Steponkus, P.L</creatorcontrib><creatorcontrib>Uemura, M</creatorcontrib><creatorcontrib>Balsamo, R.A</creatorcontrib><creatorcontrib>Arvinte, T</creatorcontrib><creatorcontrib>Lynch, D.V</creatorcontrib><title>Transformation of the cryobehavior of rye protoplasts by modification of the plasma membrane lipid composition</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>The freezing tolerance of protoplasts isolated from nonacclimated rye leaves (Secale cereale L. cv Puma) was significantly altered by using a pH-induced protoplast-liposome fusion technique to modify the lipid composition of the plasma membrane. The increase in freezing tolerance was elicited by fusion with liposomes composed of either the total phospholipid fraction isolated from the plasma membrane of cold-acclimated leaves or single mono- or diunsaturated species of phosphatidylcholine (PtdCho). Of the PtdCho species tested, dilinoleoylphosphatidylcholine ([Lin2]PtdCho) and dilinolenoylphosphatidylcholine ([Lnn2]PtdCho) liposomes were the most effective; 1-palmitoyl-2-oleoylphosphatidylcholine, 1-palmitoyl-2-linoleoylphosphatidylcholine, or dioleoylphosphatidylcholine liposomes were somewhat less effective; dimyristoylphosphatidylcholine or dipalmitoylphosphatidylcholine liposomes had no effect. The increased freezing tolerance was the result of a transformation in the cryobehavior of the plasma membrane during freeze-induced osmotic contraction. In control nonacclimated protoplasts, osmotic contraction resulted in endocytotic vesiculation of the plasma membrane which was irreversible and resulted in lysis during osmotic expansion after melting of the suspending medium. In nonacclimated protoplasts fused with mono- or diunsaturated species of PtdCho, osmotic contraction resulted in the reversible formation of exocytotic extrusions of the plasma membrane--as normally occurs in protoplasts isolated from cold-acclimated leaves (acclimated protoplasts). In scanning electron micrographs, the morphology of the extrusions of nonacclimated protoplasts fused with [Lin2]PtdCho was virtually indistinguishable from that of the extrusions formed in acclimated protoplasts. These studies provide direct evidence that changes in the lipid composition of the plasma membrane are causally related to one facet of the cold-acclimation process.</description><subject>Acclimatization</subject><subject>Biological and medical sciences</subject><subject>Biological Sciences: Botany</subject><subject>Cell membranes</subject><subject>Cell nucleus</subject><subject>CELL STRUCTURE</subject><subject>Cell structures and functions</subject><subject>Chemical composition</subject><subject>COLD</subject><subject>CONGELACION</subject><subject>CONGELATION</subject><subject>ESTRUCTURA CELULAR</subject><subject>FEUILLE</subject><subject>FREEZING</subject><subject>FRIO</subject><subject>FROID</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>HOJAS</subject><subject>LEAVES</subject><subject>LIPIDE</subject><subject>LIPIDOS</subject><subject>LIPIDS</subject><subject>Liposomes</subject><subject>Molecular and cellular biology</subject><subject>OSMOSE</subject><subject>OSMOSIS</subject><subject>Phospholipids</subject><subject>Plasma physics</subject><subject>PROTOPLASTE</subject><subject>PROTOPLASTO</subject><subject>PROTOPLASTS</subject><subject>RESISTANCE A LA TEMPERATURE</subject><subject>RESISTENCIA A LA TEMPERATURA</subject><subject>Rye</subject><subject>SECALE CEREALE</subject><subject>STRUCTURE CELLULAIRE</subject><subject>TEMPERATURE RESISTANCE</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNp9kc1v3CAQxVHVqtmmPVfqofKhUk7eDGAwHHqIon5JkXpockYYQ5bINi6wUfe_L9ZuN9lLT2h4vzcz8BB6j2GNoaWX86TTWrA1oWsJhL9AKwwS17yR8BKtAEhbi4Y0Z-hNSg8AIJmA1-gMcyabUq7QdBv1lFyIo84-TFVwVd7YysRd6OxGP_oQl7u4s9UcQw7zoFNOVberxtB7582JbVFHXY127EpbWw1-9n1lwjiH5BfwLXrl9JDsu8N5ju6-frm9_l7f_Pz24_rqpjYMZK6NZJ3mIIRpMHSO9K2zYCyVkhsCjltiemk6SdseoKGaQddYqjGjuJQO6Dn6vO87b7vR9sZOOepBzdGPOu5U0F6dKpPfqPvwqIggnLHivzj4Y_i9tSmr0Sdjh6G8KmyTaillgEnDC3m5J00MKUXrjkMwqCUjtWSkBFOEqiWj4vj4fLcn_hBKAT4dAJ2MHlz5SuPTkeOiEZySZ32WAf_Uk0EX_wWU2w5Dtn9yIT_syYeUQzyilIhWPolOB6XvY1nl7pcQLaFF_AuZ5sa-</recordid><startdate>19881201</startdate><enddate>19881201</enddate><creator>Steponkus, P.L</creator><creator>Uemura, M</creator><creator>Balsamo, R.A</creator><creator>Arvinte, T</creator><creator>Lynch, D.V</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19881201</creationdate><title>Transformation of the cryobehavior of rye protoplasts by modification of the plasma membrane lipid composition</title><author>Steponkus, P.L ; Uemura, M ; Balsamo, R.A ; Arvinte, T ; Lynch, D.V</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c509t-c95ba6088c410bf2d7fe0ce3996c20f6e2cd9cb937d0043a50b4e3a1531043f03</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Acclimatization</topic><topic>Biological and medical sciences</topic><topic>Biological Sciences: Botany</topic><topic>Cell membranes</topic><topic>Cell nucleus</topic><topic>CELL STRUCTURE</topic><topic>Cell structures and functions</topic><topic>Chemical composition</topic><topic>COLD</topic><topic>CONGELACION</topic><topic>CONGELATION</topic><topic>ESTRUCTURA CELULAR</topic><topic>FEUILLE</topic><topic>FREEZING</topic><topic>FRIO</topic><topic>FROID</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>HOJAS</topic><topic>LEAVES</topic><topic>LIPIDE</topic><topic>LIPIDOS</topic><topic>LIPIDS</topic><topic>Liposomes</topic><topic>Molecular and cellular biology</topic><topic>OSMOSE</topic><topic>OSMOSIS</topic><topic>Phospholipids</topic><topic>Plasma physics</topic><topic>PROTOPLASTE</topic><topic>PROTOPLASTO</topic><topic>PROTOPLASTS</topic><topic>RESISTANCE A LA TEMPERATURE</topic><topic>RESISTENCIA A LA TEMPERATURA</topic><topic>Rye</topic><topic>SECALE CEREALE</topic><topic>STRUCTURE CELLULAIRE</topic><topic>TEMPERATURE RESISTANCE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Steponkus, P.L</creatorcontrib><creatorcontrib>Uemura, M</creatorcontrib><creatorcontrib>Balsamo, R.A</creatorcontrib><creatorcontrib>Arvinte, T</creatorcontrib><creatorcontrib>Lynch, D.V</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Steponkus, P.L</au><au>Uemura, M</au><au>Balsamo, R.A</au><au>Arvinte, T</au><au>Lynch, D.V</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Transformation of the cryobehavior of rye protoplasts by modification of the plasma membrane lipid composition</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1988-12-01</date><risdate>1988</risdate><volume>85</volume><issue>23</issue><spage>9026</spage><epage>9030</epage><pages>9026-9030</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>The freezing tolerance of protoplasts isolated from nonacclimated rye leaves (Secale cereale L. cv Puma) was significantly altered by using a pH-induced protoplast-liposome fusion technique to modify the lipid composition of the plasma membrane. The increase in freezing tolerance was elicited by fusion with liposomes composed of either the total phospholipid fraction isolated from the plasma membrane of cold-acclimated leaves or single mono- or diunsaturated species of phosphatidylcholine (PtdCho). Of the PtdCho species tested, dilinoleoylphosphatidylcholine ([Lin2]PtdCho) and dilinolenoylphosphatidylcholine ([Lnn2]PtdCho) liposomes were the most effective; 1-palmitoyl-2-oleoylphosphatidylcholine, 1-palmitoyl-2-linoleoylphosphatidylcholine, or dioleoylphosphatidylcholine liposomes were somewhat less effective; dimyristoylphosphatidylcholine or dipalmitoylphosphatidylcholine liposomes had no effect. The increased freezing tolerance was the result of a transformation in the cryobehavior of the plasma membrane during freeze-induced osmotic contraction. In control nonacclimated protoplasts, osmotic contraction resulted in endocytotic vesiculation of the plasma membrane which was irreversible and resulted in lysis during osmotic expansion after melting of the suspending medium. In nonacclimated protoplasts fused with mono- or diunsaturated species of PtdCho, osmotic contraction resulted in the reversible formation of exocytotic extrusions of the plasma membrane--as normally occurs in protoplasts isolated from cold-acclimated leaves (acclimated protoplasts). In scanning electron micrographs, the morphology of the extrusions of nonacclimated protoplasts fused with [Lin2]PtdCho was virtually indistinguishable from that of the extrusions formed in acclimated protoplasts. These studies provide direct evidence that changes in the lipid composition of the plasma membrane are causally related to one facet of the cold-acclimation process.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>16594000</pmid><doi>10.1073/pnas.85.23.9026</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Proceedings of the National Academy of Sciences - PNAS, 1988-12, Vol.85 (23), p.9026-9030 |
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| source | JSTOR Archive Collection A-Z Listing; PubMed Central; Alma/SFX Local Collection; Free Full-Text Journals in Chemistry |
| subjects | Acclimatization Biological and medical sciences Biological Sciences: Botany Cell membranes Cell nucleus CELL STRUCTURE Cell structures and functions Chemical composition COLD CONGELACION CONGELATION ESTRUCTURA CELULAR FEUILLE FREEZING FRIO FROID Fundamental and applied biological sciences. Psychology HOJAS LEAVES LIPIDE LIPIDOS LIPIDS Liposomes Molecular and cellular biology OSMOSE OSMOSIS Phospholipids Plasma physics PROTOPLASTE PROTOPLASTO PROTOPLASTS RESISTANCE A LA TEMPERATURE RESISTENCIA A LA TEMPERATURA Rye SECALE CEREALE STRUCTURE CELLULAIRE TEMPERATURE RESISTANCE |
| title | Transformation of the cryobehavior of rye protoplasts by modification of the plasma membrane lipid composition |
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