SEPARATION BY PULSED-FIELD GRADIENT GEL ELECTROPHORESIS OF GIANT LINEAR PLASMIDS FROM ANTIBIOTIC-PRODUCING STRAINS OF STREPTOMYCES AND NOCARDIA

Eleven strains belonging to either Streptomyces or Nocardia were examined for the presence of giant linear plasmid(s) by pulsed-field gel electrophoresis with a 'home-made' apparatus. Such a plasmid could be detected only in S. coelicolor (where its presence is already known) and in S. rim...

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Veröffentlicht in:	Current science (Bangalore) 1989-11, Vol.58 (22), p.1235-1239
Hauptverfasser:	RATHOS, MAGGIE J., VERMA, N. C., NOTANI, N. K.
Format:	Artikel
Sprache:	eng
Schlagworte:	Antibiotics Biological and medical sciences Biotechnology Cathodes Chromosomes Diodes DNA Electrodes Electrophoresis Fundamental and applied biological sciences. Psychology Gels Genetic engineering Genetic technics Methods. Procedures. Technologies Miscellaneous Plasmids Streptomyces Synthetic digonucleotides and genes. Sequencing
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container_title	Current science (Bangalore)
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creator	RATHOS, MAGGIE J. VERMA, N. C. NOTANI, N. K.
description	Eleven strains belonging to either Streptomyces or Nocardia were examined for the presence of giant linear plasmid(s) by pulsed-field gel electrophoresis with a 'home-made' apparatus. Such a plasmid could be detected only in S. coelicolor (where its presence is already known) and in S. rimosus. The size of the S. rimosus plasmid was estimated to be 255 kb, which makes it somewhat smaller than the S. coelicolor 320 kb plasmid. Nick-translated S. rimosus plasmid DNA hybridizes mainly with the plasmid and faintly with the chromosomal DNA. It does not hybridize with either the chromosomal or plasmid DNA of S. coelicolor, suggesting that the latter is an altogether different plasmid. Since S. rimosus is an antibiotic-producing strain, there is a possibility, if not the likelihood, that the genes for antibiotic production may be located on the giant plasmid detected.
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C. ; NOTANI, N. K.</creator><creatorcontrib>RATHOS, MAGGIE J. ; VERMA, N. C. ; NOTANI, N. K.</creatorcontrib><description>Eleven strains belonging to either Streptomyces or Nocardia were examined for the presence of giant linear plasmid(s) by pulsed-field gel electrophoresis with a 'home-made' apparatus. Such a plasmid could be detected only in S. coelicolor (where its presence is already known) and in S. rimosus. The size of the S. rimosus plasmid was estimated to be 255 kb, which makes it somewhat smaller than the S. coelicolor 320 kb plasmid. Nick-translated S. rimosus plasmid DNA hybridizes mainly with the plasmid and faintly with the chromosomal DNA. It does not hybridize with either the chromosomal or plasmid DNA of S. coelicolor, suggesting that the latter is an altogether different plasmid. Since S. rimosus is an antibiotic-producing strain, there is a possibility, if not the likelihood, that the genes for antibiotic production may be located on the giant plasmid detected.</description><identifier>ISSN: 0011-3891</identifier><identifier>CODEN: CUSCAM</identifier><language>eng</language><publisher>Bangalore: CURRENT SCIENCE ASSOCIATION</publisher><subject>Antibiotics ; Biological and medical sciences ; Biotechnology ; Cathodes ; Chromosomes ; Diodes ; DNA ; Electrodes ; Electrophoresis ; Fundamental and applied biological sciences. Psychology ; Gels ; Genetic engineering ; Genetic technics ; Methods. Procedures. Technologies ; Miscellaneous ; Plasmids ; Streptomyces ; Synthetic digonucleotides and genes. 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K.</creatorcontrib><title>SEPARATION BY PULSED-FIELD GRADIENT GEL ELECTROPHORESIS OF GIANT LINEAR PLASMIDS FROM ANTIBIOTIC-PRODUCING STRAINS OF STREPTOMYCES AND NOCARDIA</title><title>Current science (Bangalore)</title><description>Eleven strains belonging to either Streptomyces or Nocardia were examined for the presence of giant linear plasmid(s) by pulsed-field gel electrophoresis with a 'home-made' apparatus. Such a plasmid could be detected only in S. coelicolor (where its presence is already known) and in S. rimosus. The size of the S. rimosus plasmid was estimated to be 255 kb, which makes it somewhat smaller than the S. coelicolor 320 kb plasmid. Nick-translated S. rimosus plasmid DNA hybridizes mainly with the plasmid and faintly with the chromosomal DNA. It does not hybridize with either the chromosomal or plasmid DNA of S. coelicolor, suggesting that the latter is an altogether different plasmid. Since S. rimosus is an antibiotic-producing strain, there is a possibility, if not the likelihood, that the genes for antibiotic production may be located on the giant plasmid detected.</description><subject>Antibiotics</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cathodes</subject><subject>Chromosomes</subject><subject>Diodes</subject><subject>DNA</subject><subject>Electrodes</subject><subject>Electrophoresis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>Genetic engineering</subject><subject>Genetic technics</subject><subject>Methods. Procedures. Technologies</subject><subject>Miscellaneous</subject><subject>Plasmids</subject><subject>Streptomyces</subject><subject>Synthetic digonucleotides and genes. 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K.</creator><general>CURRENT SCIENCE ASSOCIATION</general><general>Current Science Association</general><general>Indian Academy of Sciences</general><scope>IQODW</scope></search><sort><creationdate>19891120</creationdate><title>SEPARATION BY PULSED-FIELD GRADIENT GEL ELECTROPHORESIS OF GIANT LINEAR PLASMIDS FROM ANTIBIOTIC-PRODUCING STRAINS OF STREPTOMYCES AND NOCARDIA</title><author>RATHOS, MAGGIE J. ; VERMA, N. C. ; NOTANI, N. 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Sequencing</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>RATHOS, MAGGIE J.</creatorcontrib><creatorcontrib>VERMA, N. C.</creatorcontrib><creatorcontrib>NOTANI, N. K.</creatorcontrib><collection>Pascal-Francis</collection><jtitle>Current science (Bangalore)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>RATHOS, MAGGIE J.</au><au>VERMA, N. C.</au><au>NOTANI, N. 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Nick-translated S. rimosus plasmid DNA hybridizes mainly with the plasmid and faintly with the chromosomal DNA. It does not hybridize with either the chromosomal or plasmid DNA of S. coelicolor, suggesting that the latter is an altogether different plasmid. Since S. rimosus is an antibiotic-producing strain, there is a possibility, if not the likelihood, that the genes for antibiotic production may be located on the giant plasmid detected.</abstract><cop>Bangalore</cop><pub>CURRENT SCIENCE ASSOCIATION</pub><tpages>5</tpages></addata></record>
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source	Jstor Complete Legacy; EZB-FREE-00999 freely available EZB journals
subjects	Antibiotics Biological and medical sciences Biotechnology Cathodes Chromosomes Diodes DNA Electrodes Electrophoresis Fundamental and applied biological sciences. Psychology Gels Genetic engineering Genetic technics Methods. Procedures. Technologies Miscellaneous Plasmids Streptomyces Synthetic digonucleotides and genes. Sequencing
title	SEPARATION BY PULSED-FIELD GRADIENT GEL ELECTROPHORESIS OF GIANT LINEAR PLASMIDS FROM ANTIBIOTIC-PRODUCING STRAINS OF STREPTOMYCES AND NOCARDIA
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