Coupling of Nonpolymerizable Monomeric Actin to the F-Actin Binding Region of the Myosin Head

Polymerizations of skeletal G-actin induced by salt and myosin subfragment 1 (S-1) were suppressed by reaction of G-actin with m-maleimidobenzoyl-N-hydroxysuccinimide ester. The G-actin derivative, containing few intramolecular crosslinks and a free maleimide group, was covalently coupled in solutio...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1989-08, Vol.86 (16), p.6028-6032
Hauptverfasser:	Bettache, Nadir, Bertrand, Raoul, Kassab, Ridha
Format:	Artikel
Sprache:	eng
Schlagworte:	Actins Actins - metabolism Analytical, structural and metabolic biochemistry Animals Biochemistry Biological and medical sciences Contractile proteins Crosslinking Electrophoresis Electrophoresis, Polyacrylamide Gel Esters Fundamental and applied biological sciences. Psychology Gels Holoproteins Kinetics Life Sciences Molecular Weight Muscles - metabolism myosin Myosins - metabolism Nucleotides Peptide Fragments - analysis Polymerization Protein Binding Proteins Rabbits rats skeletal muscle Teeth Thiols Trypsin
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