Frequency of dual infections of corneas with HIV-1 and HHV-6
In the current study, 35 pairs of corneas from asymptomatic carriers of HIV-1 and ten pairs from AIDS patients were analyzed for the presence of HIV-1 and HHV-6. The tissues were evaluated for viral antigens, transcripts, DNA sequences and intact and infectious virus. Three corneas from two asymptom...
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| Veröffentlicht in: | Current eye research 1992, Vol.11 (4), p.315-323 |
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| creator | Qavi, Hamida Q. Green, Mary T. SeGall, Gary K. Lewis, Dorothy E. Hollinger, F. Blaine |
| description | In the current study, 35 pairs of corneas from asymptomatic carriers of HIV-1 and ten pairs from AIDS patients were analyzed for the presence of HIV-1 and HHV-6. The tissues were evaluated for viral antigens, transcripts, DNA sequences and intact and infectious virus. Three corneas from two asymptomatic carriers of HIV-1 and three corneas from two AIDS patients were culture positive for HIV-1. One of the three HIV-1 positive corneas from an asymptomatic HIV-1 carrier also was culture positive for HHV-6. Two of the tissue culture positive corneas from asymptomatic HIV-1 carriers and two from AIDS patients also tested positive for HIV-1 transcriptional activity by in situ hybridization. The label denoting the transcriptional activity was limited to stromal keratocytes. Most significantly, we were able to demonstrate the presence of HIV-1 particle(s) in sections and cultured PBMC from one of the HIV-1 culture positive corneas. PBMC from the same cornea also contained herpes virus particles. This report strengthens our earlier findings that HIV-1 and HHV-6 can invade corneal tissue, which emphasizes the importance of vigorous screening of corneal donors, specifically donors with HIV-1 exposure. |
| doi_str_mv | 10.3109/02713689209001785 |
| format | Article |
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Blaine</creator><creatorcontrib>Qavi, Hamida Q. ; Green, Mary T. ; SeGall, Gary K. ; Lewis, Dorothy E. ; Hollinger, F. Blaine</creatorcontrib><description>In the current study, 35 pairs of corneas from asymptomatic carriers of HIV-1 and ten pairs from AIDS patients were analyzed for the presence of HIV-1 and HHV-6. The tissues were evaluated for viral antigens, transcripts, DNA sequences and intact and infectious virus. Three corneas from two asymptomatic carriers of HIV-1 and three corneas from two AIDS patients were culture positive for HIV-1. One of the three HIV-1 positive corneas from an asymptomatic HIV-1 carrier also was culture positive for HHV-6. Two of the tissue culture positive corneas from asymptomatic HIV-1 carriers and two from AIDS patients also tested positive for HIV-1 transcriptional activity by in situ hybridization. The label denoting the transcriptional activity was limited to stromal keratocytes. Most significantly, we were able to demonstrate the presence of HIV-1 particle(s) in sections and cultured PBMC from one of the HIV-1 culture positive corneas. PBMC from the same cornea also contained herpes virus particles. This report strengthens our earlier findings that HIV-1 and HHV-6 can invade corneal tissue, which emphasizes the importance of vigorous screening of corneal donors, specifically donors with HIV-1 exposure.</description><identifier>ISSN: 0271-3683</identifier><identifier>EISSN: 1460-2202</identifier><identifier>DOI: 10.3109/02713689209001785</identifier><identifier>PMID: 1326427</identifier><identifier>CODEN: CEYRDM</identifier><language>eng</language><publisher>Lisse: Informa UK Ltd</publisher><subject>Acquired Immunodeficiency Syndrome - diagnosis ; AIDS/HIV ; Antigens, Viral - analysis ; Autoradiography ; Base Sequence ; Biological and medical sciences ; Corneal Diseases - diagnosis ; Corneal Diseases - microbiology ; DNA, Viral - analysis ; Eye Infections, Viral - diagnosis ; Eye Infections, Viral - microbiology ; Gene Expression Regulation, Viral - genetics ; Herpesviridae Infections - diagnosis ; Herpesviridae Infections - microbiology ; Herpesvirus 6, Human - isolation & purification ; Herpesvirus 6, Human - ultrastructure ; HIV Infections - diagnosis ; HIV Infections - microbiology ; HIV-1 - isolation & purification ; HIV-1 - ultrastructure ; Human viral diseases ; Humans ; Infectious diseases ; Medical sciences ; Molecular Sequence Data ; Polymerase Chain Reaction ; Viral diseases ; Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</subject><ispartof>Current eye research, 1992, Vol.11 (4), p.315-323</ispartof><rights>1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted 1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c430t-ba9bb715ee2cf788b14661694e2d42688fca5bdc55a4369aef23512d391a466f3</citedby><cites>FETCH-LOGICAL-c430t-ba9bb715ee2cf788b14661694e2d42688fca5bdc55a4369aef23512d391a466f3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.tandfonline.com/doi/pdf/10.3109/02713689209001785$$EPDF$$P50$$Ginformaworld$$H</linktopdf><linktohtml>$$Uhttps://www.tandfonline.com/doi/full/10.3109/02713689209001785$$EHTML$$P50$$Ginformaworld$$H</linktohtml><link.rule.ids>314,776,780,4010,27900,27901,27902,59620,59726,60409,60515,61194,61229,61375,61410</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5613301$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1326427$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Qavi, Hamida Q.</creatorcontrib><creatorcontrib>Green, Mary T.</creatorcontrib><creatorcontrib>SeGall, Gary K.</creatorcontrib><creatorcontrib>Lewis, Dorothy E.</creatorcontrib><creatorcontrib>Hollinger, F. Blaine</creatorcontrib><title>Frequency of dual infections of corneas with HIV-1 and HHV-6</title><title>Current eye research</title><addtitle>Curr Eye Res</addtitle><description>In the current study, 35 pairs of corneas from asymptomatic carriers of HIV-1 and ten pairs from AIDS patients were analyzed for the presence of HIV-1 and HHV-6. The tissues were evaluated for viral antigens, transcripts, DNA sequences and intact and infectious virus. Three corneas from two asymptomatic carriers of HIV-1 and three corneas from two AIDS patients were culture positive for HIV-1. One of the three HIV-1 positive corneas from an asymptomatic HIV-1 carrier also was culture positive for HHV-6. Two of the tissue culture positive corneas from asymptomatic HIV-1 carriers and two from AIDS patients also tested positive for HIV-1 transcriptional activity by in situ hybridization. The label denoting the transcriptional activity was limited to stromal keratocytes. Most significantly, we were able to demonstrate the presence of HIV-1 particle(s) in sections and cultured PBMC from one of the HIV-1 culture positive corneas. PBMC from the same cornea also contained herpes virus particles. This report strengthens our earlier findings that HIV-1 and HHV-6 can invade corneal tissue, which emphasizes the importance of vigorous screening of corneal donors, specifically donors with HIV-1 exposure.</description><subject>Acquired Immunodeficiency Syndrome - diagnosis</subject><subject>AIDS/HIV</subject><subject>Antigens, Viral - analysis</subject><subject>Autoradiography</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Corneal Diseases - diagnosis</subject><subject>Corneal Diseases - microbiology</subject><subject>DNA, Viral - analysis</subject><subject>Eye Infections, Viral - diagnosis</subject><subject>Eye Infections, Viral - microbiology</subject><subject>Gene Expression Regulation, Viral - genetics</subject><subject>Herpesviridae Infections - diagnosis</subject><subject>Herpesviridae Infections - microbiology</subject><subject>Herpesvirus 6, Human - isolation & purification</subject><subject>Herpesvirus 6, Human - ultrastructure</subject><subject>HIV Infections - diagnosis</subject><subject>HIV Infections - microbiology</subject><subject>HIV-1 - isolation & purification</subject><subject>HIV-1 - ultrastructure</subject><subject>Human viral diseases</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Medical sciences</subject><subject>Molecular Sequence Data</subject><subject>Polymerase Chain Reaction</subject><subject>Viral diseases</subject><subject>Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</subject><issn>0271-3683</issn><issn>1460-2202</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEFLw0AQhRdRaq3-AA9CDuIturObbBL0IsXaQsGL9homm12akmbrbkLpv3dDqyJCTwPzvje8eYRcA73nQLMHyhLgIs0YzSiFJI1PyBAiQUPGKDslw14PPcDPyYVzK0r7RTQgA-BMRCwZkqeJVZ-dauQuMDooO6yDqtFKtpVpXL-SxjYKXbCt2mUwnS1CCLApg-l0EYpLcqaxdurqMEfkY_LyPp6G87fX2fh5HsqI0zYsMCuKBGKlmNRJmhY-ogCRRYqVERNpqiXGRSnjGCMuMlSa8RhYyTNAT2o-Inf7uxtrfFjX5uvKSVXX2CjTuTzhkDAGmQdhD0prnLNK5xtbrdHucqB531j-rzHvuTkc74q1Kn8d-4q8fnvQ0UmstcVGVu4HiwVwTsFjT3vM12fsGrfG1mXe4q429tvDj6V4_GNfKqzbpUSr8pXpbOPrPfLDF_oTlSo</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Qavi, Hamida Q.</creator><creator>Green, Mary T.</creator><creator>SeGall, Gary K.</creator><creator>Lewis, Dorothy E.</creator><creator>Hollinger, F. Blaine</creator><general>Informa UK Ltd</general><general>Taylor & Francis</general><general>Swets & Zeitlinger</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1992</creationdate><title>Frequency of dual infections of corneas with HIV-1 and HHV-6</title><author>Qavi, Hamida Q. ; Green, Mary T. ; SeGall, Gary K. ; Lewis, Dorothy E. ; Hollinger, F. Blaine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c430t-ba9bb715ee2cf788b14661694e2d42688fca5bdc55a4369aef23512d391a466f3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>Acquired Immunodeficiency Syndrome - diagnosis</topic><topic>AIDS/HIV</topic><topic>Antigens, Viral - analysis</topic><topic>Autoradiography</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Corneal Diseases - diagnosis</topic><topic>Corneal Diseases - microbiology</topic><topic>DNA, Viral - analysis</topic><topic>Eye Infections, Viral - diagnosis</topic><topic>Eye Infections, Viral - microbiology</topic><topic>Gene Expression Regulation, Viral - genetics</topic><topic>Herpesviridae Infections - diagnosis</topic><topic>Herpesviridae Infections - microbiology</topic><topic>Herpesvirus 6, Human - isolation & purification</topic><topic>Herpesvirus 6, Human - ultrastructure</topic><topic>HIV Infections - diagnosis</topic><topic>HIV Infections - microbiology</topic><topic>HIV-1 - isolation & purification</topic><topic>HIV-1 - ultrastructure</topic><topic>Human viral diseases</topic><topic>Humans</topic><topic>Infectious diseases</topic><topic>Medical sciences</topic><topic>Molecular Sequence Data</topic><topic>Polymerase Chain Reaction</topic><topic>Viral diseases</topic><topic>Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Qavi, Hamida Q.</creatorcontrib><creatorcontrib>Green, Mary T.</creatorcontrib><creatorcontrib>SeGall, Gary K.</creatorcontrib><creatorcontrib>Lewis, Dorothy E.</creatorcontrib><creatorcontrib>Hollinger, F. Blaine</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Current eye research</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Qavi, Hamida Q.</au><au>Green, Mary T.</au><au>SeGall, Gary K.</au><au>Lewis, Dorothy E.</au><au>Hollinger, F. Blaine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Frequency of dual infections of corneas with HIV-1 and HHV-6</atitle><jtitle>Current eye research</jtitle><addtitle>Curr Eye Res</addtitle><date>1992</date><risdate>1992</risdate><volume>11</volume><issue>4</issue><spage>315</spage><epage>323</epage><pages>315-323</pages><issn>0271-3683</issn><eissn>1460-2202</eissn><coden>CEYRDM</coden><abstract>In the current study, 35 pairs of corneas from asymptomatic carriers of HIV-1 and ten pairs from AIDS patients were analyzed for the presence of HIV-1 and HHV-6. The tissues were evaluated for viral antigens, transcripts, DNA sequences and intact and infectious virus. Three corneas from two asymptomatic carriers of HIV-1 and three corneas from two AIDS patients were culture positive for HIV-1. One of the three HIV-1 positive corneas from an asymptomatic HIV-1 carrier also was culture positive for HHV-6. Two of the tissue culture positive corneas from asymptomatic HIV-1 carriers and two from AIDS patients also tested positive for HIV-1 transcriptional activity by in situ hybridization. The label denoting the transcriptional activity was limited to stromal keratocytes. Most significantly, we were able to demonstrate the presence of HIV-1 particle(s) in sections and cultured PBMC from one of the HIV-1 culture positive corneas. PBMC from the same cornea also contained herpes virus particles. This report strengthens our earlier findings that HIV-1 and HHV-6 can invade corneal tissue, which emphasizes the importance of vigorous screening of corneal donors, specifically donors with HIV-1 exposure.</abstract><cop>Lisse</cop><pub>Informa UK Ltd</pub><pmid>1326427</pmid><doi>10.3109/02713689209001785</doi><tpages>9</tpages></addata></record> |
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| source | Taylor & Francis:Master (3349 titles); MEDLINE; Taylor & Francis Medical Library - CRKN |
| subjects | Acquired Immunodeficiency Syndrome - diagnosis AIDS/HIV Antigens, Viral - analysis Autoradiography Base Sequence Biological and medical sciences Corneal Diseases - diagnosis Corneal Diseases - microbiology DNA, Viral - analysis Eye Infections, Viral - diagnosis Eye Infections, Viral - microbiology Gene Expression Regulation, Viral - genetics Herpesviridae Infections - diagnosis Herpesviridae Infections - microbiology Herpesvirus 6, Human - isolation & purification Herpesvirus 6, Human - ultrastructure HIV Infections - diagnosis HIV Infections - microbiology HIV-1 - isolation & purification HIV-1 - ultrastructure Human viral diseases Humans Infectious diseases Medical sciences Molecular Sequence Data Polymerase Chain Reaction Viral diseases Viral diseases with cutaneous or mucosal lesions and viral diseases of the eye |
| title | Frequency of dual infections of corneas with HIV-1 and HHV-6 |
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