EFFECTS OF WASHING ON POSITIVE STAINING

EFFECTS OF WASHING ON POSITIVE STAINING

When sections embedded in epoxy resin were conventionally stained with lead citrate and washed very briefly, about 1 sec, a very high staining intensity was attained. However, the staining intensity was quickly reduced by washing 10 min. When uranyl acetate-stained sections were washed for 1 sec, a...

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Veröffentlicht in:ACTA HISTOCHEMICA ET CYTOCHEMICA 1992, Vol.25(1-2), pp.143-146
1. Verfasser: TOKUYASU, KIYOTERU
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Biological and medical sciences
Diverse techniques
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
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description When sections embedded in epoxy resin were conventionally stained with lead citrate and washed very briefly, about 1 sec, a very high staining intensity was attained. However, the staining intensity was quickly reduced by washing 10 min. When uranyl acetate-stained sections were washed for 1 sec, a much lower intensity was obtained. Nevertheless, a significant reduction in staining intensity occurred when washing was prolonged to 10 min. These observations indicated that not only in staining of frozen sections or sections embedded in hydrophilic resins but also those embedded in more hydrophobic resins, the staining intensity is closely related to the degree of washing, particularly in lead-staining.
doi_str_mv 10.1267/ahc.25.143
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source J-STAGE Free; Open Access Titles of Japan; EZB-FREE-00999 freely available EZB journals; Free Full-Text Journals in Chemistry
subjects Biological and medical sciences
Diverse techniques
Fundamental and applied biological sciences. Psychology
Molecular and cellular biology
title EFFECTS OF WASHING ON POSITIVE STAINING
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