β-Oxidation of 12( S)-hydroxy-5,8,10,14-eicosatetraenoic acid by MOLT-4 Lymphocytes
MOLT-4 lymphocytes metabolize 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12( S)-HETE via β-oxidation with retention of the hydroxyl group at the ω9 carbon atom. The isolation of 6-hydroxy-4,8-tetradecadienoic acid documents that these cells have the capacity to catabolize the conjugated diene syste...
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| Veröffentlicht in: | Archives of biochemistry and biophysics 1992, Vol.292 (1), p.112-120 |
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| creator | Hadjiagapiou, Christos Travers, Jeffrey Fertel, Richard Sprecher, Howard |
| description | MOLT-4 lymphocytes metabolize 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12(
S)-HETE via β-oxidation with retention of the hydroxyl group at the ω9 carbon atom. The isolation of 6-hydroxy-4,8-tetradecadienoic acid documents that these cells have the capacity to catabolize the conjugated diene system. 12(
S)-HETE was also metabolized to 3,12-dihydroxy-8,10,14-eicosatrienoic acid and 1,9-dihydroxy-5,7,11-heptadecatriene as well as to 17- and 19-carbon aldehydes. When MOLT-4 cells were incubated with the β-oxidation product, 10-hydroxy-6,8,12-octadecatrienoic acid, it was in part further catabolized but in addition it served as an anabolic precursor as defined by the accumulation 3,12-dihydroxy-8,10,14-eicosatrienoic acid as well as 1,11-dihydroxy-7,9,13-nonadecatriene. Neither 10-hydroxy-6,8,12-octadecatrienoic acid nor 13-hydroxy-5,8,11-octadecatrienic acid was as potent in inhibiting phytohemagglutin-induced lymphocyte mitogenesis as were their parent compounds—i.e., 12(
S)- and 15(
S)-HETE. These findings argue against the hypothesis that β-oxidation products of 12(
S)- and 15(
S)-HETE are the potential modulators of lymphocyte function. However, neither the pathway for synthesis, nor the role of odd chain aldehydes and diols as potential lipid mediators was determined in this study. |
| doi_str_mv | 10.1016/0003-9861(92)90058-5 |
| format | Article |
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S)-HETE via β-oxidation with retention of the hydroxyl group at the ω9 carbon atom. The isolation of 6-hydroxy-4,8-tetradecadienoic acid documents that these cells have the capacity to catabolize the conjugated diene system. 12(
S)-HETE was also metabolized to 3,12-dihydroxy-8,10,14-eicosatrienoic acid and 1,9-dihydroxy-5,7,11-heptadecatriene as well as to 17- and 19-carbon aldehydes. When MOLT-4 cells were incubated with the β-oxidation product, 10-hydroxy-6,8,12-octadecatrienoic acid, it was in part further catabolized but in addition it served as an anabolic precursor as defined by the accumulation 3,12-dihydroxy-8,10,14-eicosatrienoic acid as well as 1,11-dihydroxy-7,9,13-nonadecatriene. Neither 10-hydroxy-6,8,12-octadecatrienoic acid nor 13-hydroxy-5,8,11-octadecatrienic acid was as potent in inhibiting phytohemagglutin-induced lymphocyte mitogenesis as were their parent compounds—i.e., 12(
S)- and 15(
S)-HETE. These findings argue against the hypothesis that β-oxidation products of 12(
S)- and 15(
S)-HETE are the potential modulators of lymphocyte function. However, neither the pathway for synthesis, nor the role of odd chain aldehydes and diols as potential lipid mediators was determined in this study.</description><identifier>ISSN: 0003-9861</identifier><identifier>EISSN: 1096-0384</identifier><identifier>DOI: 10.1016/0003-9861(92)90058-5</identifier><identifier>PMID: 1727629</identifier><identifier>CODEN: ABBIA4</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>12-Hydroxy-5,8,10,14-eicosatetraenoic Acid ; Adult ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Cell Line ; Fatty acids ; Fundamental and applied biological sciences. Psychology ; Humans ; Hydroxyeicosatetraenoic Acids - chemistry ; Hydroxyeicosatetraenoic Acids - metabolism ; Hydroxyeicosatetraenoic Acids - pharmacology ; Lipids ; Lymphocyte Activation - drug effects ; lymphocytes T ; MOLT-4 cells ; Other biological molecules ; Oxidation-Reduction ; T-Lymphocytes - drug effects ; T-Lymphocytes - metabolism</subject><ispartof>Archives of biochemistry and biophysics, 1992, Vol.292 (1), p.112-120</ispartof><rights>1992</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-ada40d8c862781d3f00b175923932f12444ebc37d95c84f461735368af573143</citedby><cites>FETCH-LOGICAL-c417t-ada40d8c862781d3f00b175923932f12444ebc37d95c84f461735368af573143</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0003-9861(92)90058-5$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,4010,27904,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5159733$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1727629$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Hadjiagapiou, Christos</creatorcontrib><creatorcontrib>Travers, Jeffrey</creatorcontrib><creatorcontrib>Fertel, Richard</creatorcontrib><creatorcontrib>Sprecher, Howard</creatorcontrib><title>β-Oxidation of 12( S)-hydroxy-5,8,10,14-eicosatetraenoic acid by MOLT-4 Lymphocytes</title><title>Archives of biochemistry and biophysics</title><addtitle>Arch Biochem Biophys</addtitle><description>MOLT-4 lymphocytes metabolize 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12(
S)-HETE via β-oxidation with retention of the hydroxyl group at the ω9 carbon atom. The isolation of 6-hydroxy-4,8-tetradecadienoic acid documents that these cells have the capacity to catabolize the conjugated diene system. 12(
S)-HETE was also metabolized to 3,12-dihydroxy-8,10,14-eicosatrienoic acid and 1,9-dihydroxy-5,7,11-heptadecatriene as well as to 17- and 19-carbon aldehydes. When MOLT-4 cells were incubated with the β-oxidation product, 10-hydroxy-6,8,12-octadecatrienoic acid, it was in part further catabolized but in addition it served as an anabolic precursor as defined by the accumulation 3,12-dihydroxy-8,10,14-eicosatrienoic acid as well as 1,11-dihydroxy-7,9,13-nonadecatriene. Neither 10-hydroxy-6,8,12-octadecatrienoic acid nor 13-hydroxy-5,8,11-octadecatrienic acid was as potent in inhibiting phytohemagglutin-induced lymphocyte mitogenesis as were their parent compounds—i.e., 12(
S)- and 15(
S)-HETE. These findings argue against the hypothesis that β-oxidation products of 12(
S)- and 15(
S)-HETE are the potential modulators of lymphocyte function. However, neither the pathway for synthesis, nor the role of odd chain aldehydes and diols as potential lipid mediators was determined in this study.</description><subject>12-Hydroxy-5,8,10,14-eicosatetraenoic Acid</subject><subject>Adult</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Cell Line</subject><subject>Fatty acids</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Hydroxyeicosatetraenoic Acids - chemistry</subject><subject>Hydroxyeicosatetraenoic Acids - metabolism</subject><subject>Hydroxyeicosatetraenoic Acids - pharmacology</subject><subject>Lipids</subject><subject>Lymphocyte Activation - drug effects</subject><subject>lymphocytes T</subject><subject>MOLT-4 cells</subject><subject>Other biological molecules</subject><subject>Oxidation-Reduction</subject><subject>T-Lymphocytes - drug effects</subject><subject>T-Lymphocytes - metabolism</subject><issn>0003-9861</issn><issn>1096-0384</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1992</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1uEzEURi1EVULgDUCaBapaKQZf_3uDVFVAkYKyIHvLsT2qUTIO9gR1XosH6TN1hkRlB6u7uOf7dO9B6A2Q90BAfiCEMGy0hEtDrwwhQmPxDM2AGIkJ0_w5mj0hL9DLWn8QAsAlPUfnoKiS1MzQ-uE3Xt2n4PqUuya3DdDL5vsVvhtCyfcDFgu9ALIAjmPyubo-9sXFLiffOJ9Csxmab6vlGvNmOez2d9kPfayv0FnrtjW-Ps05Wn_-tL65xcvVl68310vsOageu-A4CdprSZWGwFpCNqCEocww2gLlnMeNZyoY4TVvuQTFBJPatUIx4GyOLo61-5J_HmLt7S5VH7db18V8qHb8kWtJ2H9BmJrpSM4RP4K-5FpLbO2-pJ0rgwViJ-l2Mmono9ZQ-0e6FWPs7an_sNnF8Dd0tDzu3532rnq3bYvrfKpPmABhFJvO_HjE4ujsV4rFVp9i52NIJfrehpz-fccjGv-ZJA</recordid><startdate>1992</startdate><enddate>1992</enddate><creator>Hadjiagapiou, Christos</creator><creator>Travers, Jeffrey</creator><creator>Fertel, Richard</creator><creator>Sprecher, Howard</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T5</scope><scope>8FD</scope><scope>FR3</scope><scope>H94</scope><scope>M7Z</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>1992</creationdate><title>β-Oxidation of 12( S)-hydroxy-5,8,10,14-eicosatetraenoic acid by MOLT-4 Lymphocytes</title><author>Hadjiagapiou, Christos ; Travers, Jeffrey ; Fertel, Richard ; Sprecher, Howard</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-ada40d8c862781d3f00b175923932f12444ebc37d95c84f461735368af573143</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1992</creationdate><topic>12-Hydroxy-5,8,10,14-eicosatetraenoic Acid</topic><topic>Adult</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Cell Line</topic><topic>Fatty acids</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Humans</topic><topic>Hydroxyeicosatetraenoic Acids - chemistry</topic><topic>Hydroxyeicosatetraenoic Acids - metabolism</topic><topic>Hydroxyeicosatetraenoic Acids - pharmacology</topic><topic>Lipids</topic><topic>Lymphocyte Activation - drug effects</topic><topic>lymphocytes T</topic><topic>MOLT-4 cells</topic><topic>Other biological molecules</topic><topic>Oxidation-Reduction</topic><topic>T-Lymphocytes - drug effects</topic><topic>T-Lymphocytes - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Hadjiagapiou, Christos</creatorcontrib><creatorcontrib>Travers, Jeffrey</creatorcontrib><creatorcontrib>Fertel, Richard</creatorcontrib><creatorcontrib>Sprecher, Howard</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Immunology Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Biochemistry Abstracts 1</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Archives of biochemistry and biophysics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Hadjiagapiou, Christos</au><au>Travers, Jeffrey</au><au>Fertel, Richard</au><au>Sprecher, Howard</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>β-Oxidation of 12( S)-hydroxy-5,8,10,14-eicosatetraenoic acid by MOLT-4 Lymphocytes</atitle><jtitle>Archives of biochemistry and biophysics</jtitle><addtitle>Arch Biochem Biophys</addtitle><date>1992</date><risdate>1992</risdate><volume>292</volume><issue>1</issue><spage>112</spage><epage>120</epage><pages>112-120</pages><issn>0003-9861</issn><eissn>1096-0384</eissn><coden>ABBIA4</coden><abstract>MOLT-4 lymphocytes metabolize 12-hydroxy-5,8,10,14-eicosatetraenoic acid (12(
S)-HETE via β-oxidation with retention of the hydroxyl group at the ω9 carbon atom. The isolation of 6-hydroxy-4,8-tetradecadienoic acid documents that these cells have the capacity to catabolize the conjugated diene system. 12(
S)-HETE was also metabolized to 3,12-dihydroxy-8,10,14-eicosatrienoic acid and 1,9-dihydroxy-5,7,11-heptadecatriene as well as to 17- and 19-carbon aldehydes. When MOLT-4 cells were incubated with the β-oxidation product, 10-hydroxy-6,8,12-octadecatrienoic acid, it was in part further catabolized but in addition it served as an anabolic precursor as defined by the accumulation 3,12-dihydroxy-8,10,14-eicosatrienoic acid as well as 1,11-dihydroxy-7,9,13-nonadecatriene. Neither 10-hydroxy-6,8,12-octadecatrienoic acid nor 13-hydroxy-5,8,11-octadecatrienic acid was as potent in inhibiting phytohemagglutin-induced lymphocyte mitogenesis as were their parent compounds—i.e., 12(
S)- and 15(
S)-HETE. These findings argue against the hypothesis that β-oxidation products of 12(
S)- and 15(
S)-HETE are the potential modulators of lymphocyte function. However, neither the pathway for synthesis, nor the role of odd chain aldehydes and diols as potential lipid mediators was determined in this study.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>1727629</pmid><doi>10.1016/0003-9861(92)90058-5</doi><tpages>9</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0003-9861 |
| ispartof | Archives of biochemistry and biophysics, 1992, Vol.292 (1), p.112-120 |
| issn | 0003-9861 1096-0384 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_5159733 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | 12-Hydroxy-5,8,10,14-eicosatetraenoic Acid Adult Analytical, structural and metabolic biochemistry Biological and medical sciences Cell Line Fatty acids Fundamental and applied biological sciences. Psychology Humans Hydroxyeicosatetraenoic Acids - chemistry Hydroxyeicosatetraenoic Acids - metabolism Hydroxyeicosatetraenoic Acids - pharmacology Lipids Lymphocyte Activation - drug effects lymphocytes T MOLT-4 cells Other biological molecules Oxidation-Reduction T-Lymphocytes - drug effects T-Lymphocytes - metabolism |
| title | β-Oxidation of 12( S)-hydroxy-5,8,10,14-eicosatetraenoic acid by MOLT-4 Lymphocytes |
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