Use of Site-Directed Mutagenesis to Enhance the Epitope-Shielding Effect of Covalent Modification of Proteins with Polyethylene Glycol

Modification by covalent attachment of polyethylene glycol (PEG) can reduce the immunogenicity and prolong the circulating life of proteins, but the utility of this approach for any protein is restricted by the number and distribution of PEG attachment sites (e.g., ε-amino groups of lysine residues)...

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Veröffentlicht in:	Proceedings of the National Academy of Sciences - PNAS 1991-08, Vol.88 (16), p.7185-7189
Hauptverfasser:	Hershfield, Michael S., Chaffee, Sara, Koro-Johnson, Lillian, Mary, Ann, Smith, Albert A., Short, Steven A.
Format:	Artikel
Sprache:	eng
Schlagworte:	Amino Acid Sequence Animals Antibodies Antibody Formation Antigen-Antibody Complex Antigens Base Sequence Biological and medical sciences Codons Enzyme linked immunosorbent assay Enzymes Epitopes Epitopes - genetics Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics General pharmacology Glycols Half lives Immune response Medical sciences Mice Mice, Inbred BALB C - immunology Molecular Sequence Data Mutagenesis, Site-Directed Pharmaceutical technology. Pharmaceutical industry Pharmacology. Drug treatments Polyethylene Glycols - pharmacokinetics Polyethylene Glycols - pharmacology Purine-Nucleoside Phosphorylase - genetics Purine-Nucleoside Phosphorylase - immunology Purine-Nucleoside Phosphorylase - pharmacokinetics Recombinant Proteins - immunology Recombinant Proteins - pharmacokinetics Site directed mutagenesis Succinimides - pharmacokinetics Succinimides - pharmacology
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