Separation of carbohydrate-mediated microheterogeneity of recombinant human erythropoietin by free solution capillary electrophoresis : Effects of pH, buffer type and organic additives
Free solution capillary electrophoresis has been investigated as an alternative to isoelectric focusing for the separation of the glycoforms of recombinant human erythropoietin (r-HuEPO), a primary regulator of erythropoiesis. A systematic approach was used to study the effect of pH, buffer type and...
Gespeichert in:
| Veröffentlicht in: | Journal of Chromatography A 1991-04, Vol.542 (2), p.459-471 |
|---|---|
| Hauptverfasser: | , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 471 |
|---|---|
| container_issue | 2 |
| container_start_page | 459 |
| container_title | Journal of Chromatography A |
| container_volume | 542 |
| creator | Tran, An D. Park, Sungae Lisi, Peter J. Huynh, Oanh T. Ryall, Ralph R. Lane, Philip A. |
| description | Free solution capillary electrophoresis has been investigated as an alternative to isoelectric focusing for the separation of the glycoforms of recombinant human erythropoietin (r-HuEPO), a primary regulator of erythropoiesis. A systematic approach was used to study the effect of pH, buffer type and organic modifiers on the resolution of the microheterogeneity of erythropoietin. The main factors for improving the resolution were the regulation of the electroosmotic flow of the running buffer and the reduction of solute-wall interaction. The best resolution of the glycoforms of r-HuEPO was obtained with a mixed buffer pH 4.0 (100 mM acetate phosphate, 10 h preequilibration time). |
| doi_str_mv | 10.1016/S0021-9673(01)88781-1 |
| format | Article |
| fullrecord | <record><control><sourceid>pubmed_pasca</sourceid><recordid>TN_cdi_pascalfrancis_primary_5041553</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S0021967301887811</els_id><sourcerecordid>1880188</sourcerecordid><originalsourceid>FETCH-LOGICAL-e260t-fda51b921d627eabc7dfca229c5df61c5cad451c84cd24ebdcb1ca19afc06103</originalsourceid><addsrcrecordid>eNo9kc9q3DAQxnVoSdO0jxDQoYcW6lbjXf_ZXkoJaRMI9JDcxXg0ilVsyUjagN8sj1ftZslBDPrmx4z0fUJcgvoGCtrv90rVUO3abvNZwZe-73qo4I04f5Xfifcp_VMKOtXVZ-IM-l6Vcy6e73nBiNkFL4OVhHEI42qKwtXMxpVq5OwohpEzx_DInl1eD2xkCvPgPPosx_2MXnJc8xjDEhxn5-WwShuZZQrT_riAcHHThHGVPDHlQo4hcnJJ_pDX1hYpHQYvN1_lsC_3KPO6sERvZIiP6B1JNMZl98Tpg3hrcUr88VQvxMPv64erm-ru75_bq193FdetypU12MCwq8G0dcc4UGcsYV3vqDG2BWoIzbYB6rdk6i0PhgYghB1aUi2ozYW4fBm77Ifih16im8sH9MnA0v906mMinGxETy69Yo3aQtNsCvbzBePy1CfHUSdy7Kk4XGzM2gSnQelDmPoYpj6kphXoY5gaNv8BoR6Z1A</addsrcrecordid><sourcetype>Index Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Separation of carbohydrate-mediated microheterogeneity of recombinant human erythropoietin by free solution capillary electrophoresis : Effects of pH, buffer type and organic additives</title><source>MEDLINE</source><source>Elsevier ScienceDirect Journals</source><creator>Tran, An D. ; Park, Sungae ; Lisi, Peter J. ; Huynh, Oanh T. ; Ryall, Ralph R. ; Lane, Philip A.</creator><creatorcontrib>Tran, An D. ; Park, Sungae ; Lisi, Peter J. ; Huynh, Oanh T. ; Ryall, Ralph R. ; Lane, Philip A.</creatorcontrib><description>Free solution capillary electrophoresis has been investigated as an alternative to isoelectric focusing for the separation of the glycoforms of recombinant human erythropoietin (r-HuEPO), a primary regulator of erythropoiesis. A systematic approach was used to study the effect of pH, buffer type and organic modifiers on the resolution of the microheterogeneity of erythropoietin. The main factors for improving the resolution were the regulation of the electroosmotic flow of the running buffer and the reduction of solute-wall interaction. The best resolution of the glycoforms of r-HuEPO was obtained with a mixed buffer pH 4.0 (100 mM acetate phosphate, 10 h preequilibration time).</description><identifier>ISSN: 0021-9673</identifier><identifier>DOI: 10.1016/S0021-9673(01)88781-1</identifier><identifier>PMID: 1880188</identifier><identifier>CODEN: JOCRAM</identifier><language>eng</language><publisher>Amsterdam: Elsevier B.V</publisher><subject>Acetates - pharmacology ; Analytical, structural and metabolic biochemistry ; Biological and medical sciences ; Buffers ; Capillary Action ; Chemical Phenomena ; Chemistry, Physical ; Electrophoresis - methods ; Erythropoietin - isolation & purification ; Fundamental and applied biological sciences. Psychology ; General aspects, investigation methods ; Humans ; Hydrogen-Ion Concentration ; Phosphates - pharmacology ; Proteins ; Recombinant Proteins - isolation & purification ; Solvents ; Sulfates - pharmacology</subject><ispartof>Journal of Chromatography A, 1991-04, Vol.542 (2), p.459-471</ispartof><rights>1991</rights><rights>1992 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/S0021-9673(01)88781-1$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=5041553$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/1880188$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tran, An D.</creatorcontrib><creatorcontrib>Park, Sungae</creatorcontrib><creatorcontrib>Lisi, Peter J.</creatorcontrib><creatorcontrib>Huynh, Oanh T.</creatorcontrib><creatorcontrib>Ryall, Ralph R.</creatorcontrib><creatorcontrib>Lane, Philip A.</creatorcontrib><title>Separation of carbohydrate-mediated microheterogeneity of recombinant human erythropoietin by free solution capillary electrophoresis : Effects of pH, buffer type and organic additives</title><title>Journal of Chromatography A</title><addtitle>J Chromatogr</addtitle><description>Free solution capillary electrophoresis has been investigated as an alternative to isoelectric focusing for the separation of the glycoforms of recombinant human erythropoietin (r-HuEPO), a primary regulator of erythropoiesis. A systematic approach was used to study the effect of pH, buffer type and organic modifiers on the resolution of the microheterogeneity of erythropoietin. The main factors for improving the resolution were the regulation of the electroosmotic flow of the running buffer and the reduction of solute-wall interaction. The best resolution of the glycoforms of r-HuEPO was obtained with a mixed buffer pH 4.0 (100 mM acetate phosphate, 10 h preequilibration time).</description><subject>Acetates - pharmacology</subject><subject>Analytical, structural and metabolic biochemistry</subject><subject>Biological and medical sciences</subject><subject>Buffers</subject><subject>Capillary Action</subject><subject>Chemical Phenomena</subject><subject>Chemistry, Physical</subject><subject>Electrophoresis - methods</subject><subject>Erythropoietin - isolation & purification</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>General aspects, investigation methods</subject><subject>Humans</subject><subject>Hydrogen-Ion Concentration</subject><subject>Phosphates - pharmacology</subject><subject>Proteins</subject><subject>Recombinant Proteins - isolation & purification</subject><subject>Solvents</subject><subject>Sulfates - pharmacology</subject><issn>0021-9673</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1991</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kc9q3DAQxnVoSdO0jxDQoYcW6lbjXf_ZXkoJaRMI9JDcxXg0ilVsyUjagN8sj1ftZslBDPrmx4z0fUJcgvoGCtrv90rVUO3abvNZwZe-73qo4I04f5Xfifcp_VMKOtXVZ-IM-l6Vcy6e73nBiNkFL4OVhHEI42qKwtXMxpVq5OwohpEzx_DInl1eD2xkCvPgPPosx_2MXnJc8xjDEhxn5-WwShuZZQrT_riAcHHThHGVPDHlQo4hcnJJ_pDX1hYpHQYvN1_lsC_3KPO6sERvZIiP6B1JNMZl98Tpg3hrcUr88VQvxMPv64erm-ru75_bq193FdetypU12MCwq8G0dcc4UGcsYV3vqDG2BWoIzbYB6rdk6i0PhgYghB1aUi2ozYW4fBm77Ifih16im8sH9MnA0v906mMinGxETy69Yo3aQtNsCvbzBePy1CfHUSdy7Kk4XGzM2gSnQelDmPoYpj6kphXoY5gaNv8BoR6Z1A</recordid><startdate>19910412</startdate><enddate>19910412</enddate><creator>Tran, An D.</creator><creator>Park, Sungae</creator><creator>Lisi, Peter J.</creator><creator>Huynh, Oanh T.</creator><creator>Ryall, Ralph R.</creator><creator>Lane, Philip A.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope></search><sort><creationdate>19910412</creationdate><title>Separation of carbohydrate-mediated microheterogeneity of recombinant human erythropoietin by free solution capillary electrophoresis : Effects of pH, buffer type and organic additives</title><author>Tran, An D. ; Park, Sungae ; Lisi, Peter J. ; Huynh, Oanh T. ; Ryall, Ralph R. ; Lane, Philip A.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-e260t-fda51b921d627eabc7dfca229c5df61c5cad451c84cd24ebdcb1ca19afc06103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1991</creationdate><topic>Acetates - pharmacology</topic><topic>Analytical, structural and metabolic biochemistry</topic><topic>Biological and medical sciences</topic><topic>Buffers</topic><topic>Capillary Action</topic><topic>Chemical Phenomena</topic><topic>Chemistry, Physical</topic><topic>Electrophoresis - methods</topic><topic>Erythropoietin - isolation & purification</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>General aspects, investigation methods</topic><topic>Humans</topic><topic>Hydrogen-Ion Concentration</topic><topic>Phosphates - pharmacology</topic><topic>Proteins</topic><topic>Recombinant Proteins - isolation & purification</topic><topic>Solvents</topic><topic>Sulfates - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tran, An D.</creatorcontrib><creatorcontrib>Park, Sungae</creatorcontrib><creatorcontrib>Lisi, Peter J.</creatorcontrib><creatorcontrib>Huynh, Oanh T.</creatorcontrib><creatorcontrib>Ryall, Ralph R.</creatorcontrib><creatorcontrib>Lane, Philip A.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><jtitle>Journal of Chromatography A</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tran, An D.</au><au>Park, Sungae</au><au>Lisi, Peter J.</au><au>Huynh, Oanh T.</au><au>Ryall, Ralph R.</au><au>Lane, Philip A.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Separation of carbohydrate-mediated microheterogeneity of recombinant human erythropoietin by free solution capillary electrophoresis : Effects of pH, buffer type and organic additives</atitle><jtitle>Journal of Chromatography A</jtitle><addtitle>J Chromatogr</addtitle><date>1991-04-12</date><risdate>1991</risdate><volume>542</volume><issue>2</issue><spage>459</spage><epage>471</epage><pages>459-471</pages><issn>0021-9673</issn><coden>JOCRAM</coden><abstract>Free solution capillary electrophoresis has been investigated as an alternative to isoelectric focusing for the separation of the glycoforms of recombinant human erythropoietin (r-HuEPO), a primary regulator of erythropoiesis. A systematic approach was used to study the effect of pH, buffer type and organic modifiers on the resolution of the microheterogeneity of erythropoietin. The main factors for improving the resolution were the regulation of the electroosmotic flow of the running buffer and the reduction of solute-wall interaction. The best resolution of the glycoforms of r-HuEPO was obtained with a mixed buffer pH 4.0 (100 mM acetate phosphate, 10 h preequilibration time).</abstract><cop>Amsterdam</cop><pub>Elsevier B.V</pub><pmid>1880188</pmid><doi>10.1016/S0021-9673(01)88781-1</doi><tpages>13</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0021-9673 |
| ispartof | Journal of Chromatography A, 1991-04, Vol.542 (2), p.459-471 |
| issn | 0021-9673 |
| language | eng |
| recordid | cdi_pascalfrancis_primary_5041553 |
| source | MEDLINE; Elsevier ScienceDirect Journals |
| subjects | Acetates - pharmacology Analytical, structural and metabolic biochemistry Biological and medical sciences Buffers Capillary Action Chemical Phenomena Chemistry, Physical Electrophoresis - methods Erythropoietin - isolation & purification Fundamental and applied biological sciences. Psychology General aspects, investigation methods Humans Hydrogen-Ion Concentration Phosphates - pharmacology Proteins Recombinant Proteins - isolation & purification Solvents Sulfates - pharmacology |
| title | Separation of carbohydrate-mediated microheterogeneity of recombinant human erythropoietin by free solution capillary electrophoresis : Effects of pH, buffer type and organic additives |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-20T02%3A54%3A33IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-pubmed_pasca&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Separation%20of%20carbohydrate-mediated%20microheterogeneity%20of%20recombinant%20human%20erythropoietin%20by%20free%20solution%20capillary%20electrophoresis%20:%20Effects%20of%20pH,%20buffer%20type%20and%20organic%20additives&rft.jtitle=Journal%20of%20Chromatography%20A&rft.au=Tran,%20An%20D.&rft.date=1991-04-12&rft.volume=542&rft.issue=2&rft.spage=459&rft.epage=471&rft.pages=459-471&rft.issn=0021-9673&rft.coden=JOCRAM&rft_id=info:doi/10.1016/S0021-9673(01)88781-1&rft_dat=%3Cpubmed_pasca%3E1880188%3C/pubmed_pasca%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_id=info:pmid/1880188&rft_els_id=S0021967301887811&rfr_iscdi=true |