Molecular comparison of the structural proteins encoding gene clusters of two related Lactobacillus delbrueckii bacteriophages
Virulent phage LL-H and temperate phage mv4 are two related bacteriophages of Lactobacillus delbrueckii. The gene clusters encoding structural proteins of these two phages have been sequenced and further analyzed. Six open reading frames (ORF-1 to ORF-6) were detected. Protein sequencing and Western...
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| Veröffentlicht in: | Journal of Virology 1993-06, Vol.67 (6), p.3061-3068 |
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| creator | Vasala, A. (University of Oulu, Oulu, Finland) Dupont, L Baumann, M Ritzenthaler, P Alatossava, T |
| description | Virulent phage LL-H and temperate phage mv4 are two related bacteriophages of Lactobacillus delbrueckii. The gene clusters encoding structural proteins of these two phages have been sequenced and further analyzed. Six open reading frames (ORF-1 to ORF-6) were detected. Protein sequencing and Western immunoblotting experiments confirmed that ORF-3 (g34) encoded the main capsid protein Gp34. The presence of a putative late promoter in front of the phage LL-H g34 gene was suggested by primer extension experiments. Comparative sequence analysis between phage LL-H and phage mv4 revealed striking similarities in the structure and organization of this gene cluster, suggesting that the genes encoding phage structural proteins belong to a highly conservative module |
| doi_str_mv | 10.1128/jvi.67.6.3061-3068.1993 |
| format | Article |
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(University of Oulu, Oulu, Finland) ; Dupont, L ; Baumann, M ; Ritzenthaler, P ; Alatossava, T</creator><creatorcontrib>Vasala, A. (University of Oulu, Oulu, Finland) ; Dupont, L ; Baumann, M ; Ritzenthaler, P ; Alatossava, T</creatorcontrib><description>Virulent phage LL-H and temperate phage mv4 are two related bacteriophages of Lactobacillus delbrueckii. The gene clusters encoding structural proteins of these two phages have been sequenced and further analyzed. Six open reading frames (ORF-1 to ORF-6) were detected. Protein sequencing and Western immunoblotting experiments confirmed that ORF-3 (g34) encoded the main capsid protein Gp34. The presence of a putative late promoter in front of the phage LL-H g34 gene was suggested by primer extension experiments. Comparative sequence analysis between phage LL-H and phage mv4 revealed striking similarities in the structure and organization of this gene cluster, suggesting that the genes encoding phage structural proteins belong to a highly conservative module</description><identifier>ISSN: 0022-538X</identifier><identifier>EISSN: 1098-5514</identifier><identifier>DOI: 10.1128/jvi.67.6.3061-3068.1993</identifier><identifier>PMID: 8497043</identifier><language>eng</language><publisher>Washington, DC: American Society for Microbiology</publisher><subject>Amino Acid Sequence ; BACTERIOFAGOS ; BACTERIOPHAGE ; Bacteriophages - genetics ; Base Sequence ; Biological and medical sciences ; Fundamental and applied biological sciences. Psychology ; GENE ; GENES ; Genes, Viral - genetics ; Genetics ; LACTOBACILLUS ; lactobacillus delbrueckii ; Microbiology ; Molecular Sequence Data ; Multigene Family - genetics ; Open Reading Frames - genetics ; Promoter Regions, Genetic - genetics ; PROTEINAS ; PROTEINE ; Regulatory Sequences, Nucleic Acid - genetics ; Restriction Mapping ; SECUENCIA NUCLEICA ; Sequence Homology, Amino Acid ; SEQUENCE NUCLEIQUE ; Viral Structural Proteins - genetics ; Virology</subject><ispartof>Journal of Virology, 1993-06, Vol.67 (6), p.3061-3068</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c5513-a0d08478ebb0818bbf6afa672ca56a9a37fb8c083c0532330c7a162977d2aa623</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC237642/pdf/$$EPDF$$P50$$Gpubmedcentral$$H</linktopdf><linktohtml>$$Uhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC237642/$$EHTML$$P50$$Gpubmedcentral$$H</linktohtml><link.rule.ids>230,314,727,780,784,885,27924,27925,53791,53793</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4832169$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8497043$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Vasala, A. (University of Oulu, Oulu, Finland)</creatorcontrib><creatorcontrib>Dupont, L</creatorcontrib><creatorcontrib>Baumann, M</creatorcontrib><creatorcontrib>Ritzenthaler, P</creatorcontrib><creatorcontrib>Alatossava, T</creatorcontrib><title>Molecular comparison of the structural proteins encoding gene clusters of two related Lactobacillus delbrueckii bacteriophages</title><title>Journal of Virology</title><addtitle>J Virol</addtitle><description>Virulent phage LL-H and temperate phage mv4 are two related bacteriophages of Lactobacillus delbrueckii. The gene clusters encoding structural proteins of these two phages have been sequenced and further analyzed. Six open reading frames (ORF-1 to ORF-6) were detected. Protein sequencing and Western immunoblotting experiments confirmed that ORF-3 (g34) encoded the main capsid protein Gp34. The presence of a putative late promoter in front of the phage LL-H g34 gene was suggested by primer extension experiments. Comparative sequence analysis between phage LL-H and phage mv4 revealed striking similarities in the structure and organization of this gene cluster, suggesting that the genes encoding phage structural proteins belong to a highly conservative module</description><subject>Amino Acid Sequence</subject><subject>BACTERIOFAGOS</subject><subject>BACTERIOPHAGE</subject><subject>Bacteriophages - genetics</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE</subject><subject>GENES</subject><subject>Genes, Viral - genetics</subject><subject>Genetics</subject><subject>LACTOBACILLUS</subject><subject>lactobacillus delbrueckii</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family - genetics</subject><subject>Open Reading Frames - genetics</subject><subject>Promoter Regions, Genetic - genetics</subject><subject>PROTEINAS</subject><subject>PROTEINE</subject><subject>Regulatory Sequences, Nucleic Acid - genetics</subject><subject>Restriction Mapping</subject><subject>SECUENCIA NUCLEICA</subject><subject>Sequence Homology, Amino Acid</subject><subject>SEQUENCE NUCLEIQUE</subject><subject>Viral Structural Proteins - genetics</subject><subject>Virology</subject><issn>0022-538X</issn><issn>1098-5514</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkkuLFDEUhQtRxnb0DwhiBHFXbV6VpBYuZPAFIy50wF24lbpVnTFdaZOqGdz4203bTeOs3NxAzneSHE6q6jmja8a4eX1949dKr9VaUMXqMsyata24V60YbU3dNEzer1aUcl43wnx_WD3K-ZpSJqWSZ9WZka2mUqyq359jQLcESMTF7Q6Sz3EicSDzBkme0-LmJUEguxRn9FMmOLnY-2kkI05IXFjyjCn_ddxGkjDAjD25BDfHDpwPBSA9hi4t6H54T8pmMfi428CI-XH1YICQ8clxPa-u3r_7dvGxvvzy4dPF28valSSiBtpTI7XBrqOGma4bFAygNHfQKGhB6KEzjhrhaCO4ENRpYIq3WvccQHFxXr05nLtbui32Dqe5pLK75LeQftkI3t5VJr-xY7yxXGgl9_5XR3-KPxfMs9367DAEmDAu2epGK8E1-y_IVMnTtrKA-gC6FHNOOJwew6jdV2xLxVZpq-y-4v0wdl9xcT77N8vJd-y06C-POmQHYUgwOZ9PmDSCM9UW7MUB2_hxc-sTWsjbu5cW5umBGSBaGMvnsFdfW8kkpVr8Ae0dx8Q</recordid><startdate>19930601</startdate><enddate>19930601</enddate><creator>Vasala, A. (University of Oulu, Oulu, Finland)</creator><creator>Dupont, L</creator><creator>Baumann, M</creator><creator>Ritzenthaler, P</creator><creator>Alatossava, T</creator><general>American Society for Microbiology</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>7U9</scope><scope>C1K</scope><scope>H94</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19930601</creationdate><title>Molecular comparison of the structural proteins encoding gene clusters of two related Lactobacillus delbrueckii bacteriophages</title><author>Vasala, A. (University of Oulu, Oulu, Finland) ; Dupont, L ; Baumann, M ; Ritzenthaler, P ; Alatossava, T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c5513-a0d08478ebb0818bbf6afa672ca56a9a37fb8c083c0532330c7a162977d2aa623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>BACTERIOFAGOS</topic><topic>BACTERIOPHAGE</topic><topic>Bacteriophages - genetics</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE</topic><topic>GENES</topic><topic>Genes, Viral - genetics</topic><topic>Genetics</topic><topic>LACTOBACILLUS</topic><topic>lactobacillus delbrueckii</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family - genetics</topic><topic>Open Reading Frames - genetics</topic><topic>Promoter Regions, Genetic - genetics</topic><topic>PROTEINAS</topic><topic>PROTEINE</topic><topic>Regulatory Sequences, Nucleic Acid - genetics</topic><topic>Restriction Mapping</topic><topic>SECUENCIA NUCLEICA</topic><topic>Sequence Homology, Amino Acid</topic><topic>SEQUENCE NUCLEIQUE</topic><topic>Viral Structural Proteins - genetics</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vasala, A. (University of Oulu, Oulu, Finland)</creatorcontrib><creatorcontrib>Dupont, L</creatorcontrib><creatorcontrib>Baumann, M</creatorcontrib><creatorcontrib>Ritzenthaler, P</creatorcontrib><creatorcontrib>Alatossava, T</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Virology and AIDS Abstracts</collection><collection>Environmental Sciences and Pollution Management</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Journal of Virology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vasala, A. (University of Oulu, Oulu, Finland)</au><au>Dupont, L</au><au>Baumann, M</au><au>Ritzenthaler, P</au><au>Alatossava, T</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular comparison of the structural proteins encoding gene clusters of two related Lactobacillus delbrueckii bacteriophages</atitle><jtitle>Journal of Virology</jtitle><addtitle>J Virol</addtitle><date>1993-06-01</date><risdate>1993</risdate><volume>67</volume><issue>6</issue><spage>3061</spage><epage>3068</epage><pages>3061-3068</pages><issn>0022-538X</issn><eissn>1098-5514</eissn><abstract>Virulent phage LL-H and temperate phage mv4 are two related bacteriophages of Lactobacillus delbrueckii. The gene clusters encoding structural proteins of these two phages have been sequenced and further analyzed. Six open reading frames (ORF-1 to ORF-6) were detected. Protein sequencing and Western immunoblotting experiments confirmed that ORF-3 (g34) encoded the main capsid protein Gp34. The presence of a putative late promoter in front of the phage LL-H g34 gene was suggested by primer extension experiments. Comparative sequence analysis between phage LL-H and phage mv4 revealed striking similarities in the structure and organization of this gene cluster, suggesting that the genes encoding phage structural proteins belong to a highly conservative module</abstract><cop>Washington, DC</cop><pub>American Society for Microbiology</pub><pmid>8497043</pmid><doi>10.1128/jvi.67.6.3061-3068.1993</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Amino Acid Sequence BACTERIOFAGOS BACTERIOPHAGE Bacteriophages - genetics Base Sequence Biological and medical sciences Fundamental and applied biological sciences. Psychology GENE GENES Genes, Viral - genetics Genetics LACTOBACILLUS lactobacillus delbrueckii Microbiology Molecular Sequence Data Multigene Family - genetics Open Reading Frames - genetics Promoter Regions, Genetic - genetics PROTEINAS PROTEINE Regulatory Sequences, Nucleic Acid - genetics Restriction Mapping SECUENCIA NUCLEICA Sequence Homology, Amino Acid SEQUENCE NUCLEIQUE Viral Structural Proteins - genetics Virology |
| title | Molecular comparison of the structural proteins encoding gene clusters of two related Lactobacillus delbrueckii bacteriophages |
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